Koo S.-B.-N.,Hallym |
Koo S.-B.-N.,Bio Research Center |
Cho J.-H.,Hallym |
Cho J.-H.,Bio Research Center |
And 8 more authors.
International Journal of Control and Automation | Year: 2015
The fabrication of microfluidic channels in previously proposed polymerase chain reaction (PCR) chips by using polydimethylsiloxane, polycarbonate, and poly(methyl methaaylate) is expensive. To reduce the cost of the disposable chips, a polymer film is employed on the polychlorinated biphenyl (PCB) substrate for the bottom, a carved double-sided tape is used for the wall, and a film with inlet and outlet holes is used for the ceiling of the reaction chamber The chip temperature is controlled by the heater pattern of the substrate and the thermistor on the opposite face. Commercial chip thermistors with a 1% resistance tolerance are adapted. In this paper, the effect of the thermistor calibration on PCR performance is investigated. A comparison ofresults on the band brightness of gel images indicated that the calibration seemed to deliver abetter performance. © 2015 SERSC.