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Buenos Aires, Argentina

Quintans C.J.,Halitus Instituto Medico | Donaldson M.J.,Halitus Instituto Medico | Urquiza M.F.,Halitus Instituto Medico | Carretero I.,Halitus Instituto Medico | And 3 more authors.
Reproductive BioMedicine Online | Year: 2012

A 45-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved (slow freezing in a low-sodium medium) 11 years and 7 and a half months before, when she was 33 years old. From seven metaphase-II oocytes thawed, five survived, four were fertilized after ICSI and two cleaving embryos were transferred on day 3. A diamniotic dichorionic term pregnancy was achieved, ending with the delivery of two healthy girls. As far as is known, this case represents, to date, the longest storage period of cryopreserved human oocytes resulting in a live birth. © 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. Source

Fitzgerald J.S.,University Hospital of Jena | Abad C.,Venezuelan Institute for Scientific Research | Alvarez A.M.,University of Antioquia | Mehta R.B.,Linkoping University | And 18 more authors.
Advances in Neuroimmune Biology | Year: 2011

Pregnancy is personally special to every woman expecting a child, but is also interesting from the perspective of an immunologist. During a physiological pregnancy, the mother's immune system decides to tolerate and foster an incorporated, non-self, non-dangerous organism. Whether the maternal reaction stems from deciphering the foreigness or safeness of this new individual, it is the general consensus that there is a foeto-maternal, bidirectional "dialogue" occurring and that the "messages" that are "spoken" are relayed through signaling mediators, which are capable of transmitting a functional command to a target cell. Much information dedicated to this theme has been gleaned in the past decade; however, the complex nature of cytokine networks jeopardizes clarity. In this review, we touch upon a list of mediators that are vital for reproduction. These factors are divided according to their receptor family, because this elucidates the characteristic signal transducing pathway, which is expected to mediate their signal within the target cell. The target cells of interest are the trophoblast, upon which we focus for several reasons: 1. the trophoblast represent the foetal compartment while participating in foeto-maternal interplay (e.g. while invading the decidua, trophoblasts are in constant communication with uterine, maternal immunocytes, which check and contain this function), 2. trophoblasts are responsible for foetal well-being (e.g. nutrition, protection from the environment) and 3. dysfunctional trophoblast function results in several pregnancy complications (e.g. preeclampsia, intrauterine growth retardation, miscarriage, preterm delivery). We summarize what is described in the literature on how these mediators are distributed within the reproductive tract, which cells are expressing their respective receptors (especially which trophoblast subsets) and how they modify trophoblast function (namely invasion, proliferation, differentiation and apoptosis). Furthermore, we unearth for which mediator the signal transducing pathway is verifiably used in trophoblast (ic) cells. Finally, we correlate actual biological importance of the mediator for reproduction by comparing murine knockout phenotypes and known positive and negative associations of these mediators with human pregnancy pathologies (as listed above). We expect this concise review to be useful to both basic researchers and clinicians who wish to obtain an overview of the reproductive cytokine network in respect to the trophoblast. © 2011 - IOS Press and the authors. All rights reserved. Source

Fernanda Urquiza M.,Halitus Instituto Medico | Carretero I.,Halitus Instituto Medico | Quaini F.M.,Academia Internacional de Abogados Matrimoniales | Inciarte F.,Halitus Instituto Medico | And 2 more authors.
Medicina (Argentina) | Year: 2014

A woman aged 38 was referred to this center for surrogacy treatment, after subtotal ablation of her uterus due to a severe postpartum hemorrhage. Her hormonal profiles and ovarian structure were normal. The husband proved fertile and semen analysis was normal. The carrier, a woman 39 years old, fertile with two children of her own, and a long bonding friendship with the patient. After hormonal stimulation with gonadotropins and GnRH antagonist, six mature oocytes were obtained. These originated four embryos after in vitro fertilization, three of which were transferred to the carrier, achieving a singleton pregnancy which led to the birth of a normal child, now more than a year old. A lawsuit was filed after birth requesting the baby be registered with the biological parents name. The judge granted the request based on evidence and testimonies provided, international jurisprudence history and specification in Article 19 of the Argentine Constitution: “No inhabitant of the Nation shall be obliged to do what the law does not demand .nor be deprived of what it does not prohibit”. This is an almost ideal example of the proceedings in a case of subrogation .However, we must always bear in mind the fact that in our country there is as yet no regulatory framework for these treatments, therefore there is a high probability of conflict. © 2004, (publisher). All rights reserved. Source

Carretero I.,Halitus Instituto Medico | Urquiza M.F.,Halitus Instituto Medico | Donaldson M.J.,Halitus Instituto Medico | Alonso A.G.,Halitus Instituto Medico | And 2 more authors.
Jornal Brasileiro de Reproducao Assistida | Year: 2011

Objective: To compare the results of a program of in itro fertilization with embryos cryopreserved at blastocyst tage, either by vitrification or slow cooling. atients: transfer cycles at blastocyst stage. ere considered during the years 2009 and 2010. ethods: From our database we selected the cycles corresponding o the above characteristics. The results were nalyzed using Graph Pad InStat, using the Fisher exact test. esults: There were 133 cycles corresponding to the ransfer of cryopreserved embryos in blastocyst stage, 07 cycles corresponding to the slow technique (grL) and he remaining 26 blastocysts were vitrified (grV). In the rL group there were 3.28 ± 1.18 thawed blastocysts er cycle and the grV group had 2.96 ± 1.77 with an verage of 2.35 ± 0.90 (grL) and 2.30 ± 0 97 (grV), espectively. The difference in the recovery of viable lastocysts between the two groups was not significant P = 0.4315). In grL 11 were due to suspend transfers 10.3%) while the grV 3 (11.5%) due to lack of viability f embryos recovered. In grLthere were were 39 (40.6%) linical pregnancies versus 13 in the GRV 13 (56.5%). he observed difference was not significant (P = 0.2416). onclusions: Slow Cryopreservation at blastocyst stage s used at our institution with satisfactory results. In the last wo years we started to explore the possible advantages of itrification to determine whether it would justify changing he slow protocol in use. While the results show a tendency to be better with vitrification, the number of cases studied oes not yet allow statistically valid conclusions. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida. Source

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