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Nishi-Tokyo-shi, Japan

Hori T.,Hakuju Institute for Health Science | Harakawa S.,Hakuju Institute for Health Science | Herbas S.M.,Obihiro University of Agriculture and Veterinary Medicine | Ueta Y.Y.,Obihiro University of Agriculture and Veterinary Medicine | And 2 more authors.
Lipids in Health and Disease | Year: 2012

Background: The effects of exposure to a 50 Hz electric field (EF) on plasma level of triacylglycerol, free fatty acids, total cholesterol and phospholipid and mRNA expression level of diacylglycerol acyltransferase (DGAT) 1 and 2 in liver and intestines from C57BL/6 J mice were studied. Methods: The test was based on comparison between mice post treated with 50 Hz EF of 45 kV/m intensity for 30 min per day for 11 days or without EF. DGATs mRNA expression was analyzed by real-time quantitative polymerase chain reaction. Results: There was no difference in the gene expression level of DGAT1 in liver and intestines. The DGAT2 gene expression level in liver derived from mice treated with EF was significantly lower than those in the control (P<0.001). Both plasma total cholesterol (P<0.01) and phospholipid (P<0.05) in the group exposed to EF were lower than those in the control, but there was no difference in triacylglycerol or free fatty acid levels. Conclusion: Exposure to 50 Hz EF decrease the plasma levels of total cholesterol and phospholipids, and downregulated DGAT2 mRNA expression in liver. The mechanisms for the effects of EF on lipid metabolism are not well understand yet, but altered DGAT2 activity may be involved. © 2012 Hori et al.; licensee BioMed Central Ltd. Source

Hori T.,Obihiro University of Agriculture and Veterinary Medicine | Hori T.,Hakuju Institute for Health Science | Inoue N.,Obihiro University of Agriculture and Veterinary Medicine | Suzuki H.,Obihiro University of Agriculture and Veterinary Medicine | And 2 more authors.
Bioelectromagnetics | Year: 2015

Electric fields (EFs) can reduce elevated levels of stress-related hormones in some organisms. In this study, endocrine effects of exposure to a 50Hz EF were investigated in male BALB/c mice. Specifically, plasma glucocorticoid (GC) levels were examined because GC is known to mediate the stress response in mice, including changes induced by immobilization. Mice were exposed to 50Hz EFs (at 2.5-200kV/m) for 60min. They were immobilized for the latter half (30min). At the end of exposure period, blood samples were collected and GC levels estimated by spectrofluorometry. GC levels were not influenced by EFs in absence of immobilization, but they were significantly higher in immobilized mice than in non-immobilized mice (P<0.01). Elevated GC levels induced by immobilization were significantly reduced by exposure to an EF at 10kV/m (P<0.05), and the effect of EFs at 0-10kV/m on GC levels increased in a kV/m-dependent manner (P<0.05). In contrast, following treatment with EFs at 50 and 200kV/m, GC levels were higher than those observed at 10kV/m. To assess the effect of EF treatment duration, mice were also exposed to 50Hz EFs (10kV/m) for 6, 20, or 60min. Immobilization-induced increase in GC levels was significantly suppressed by EF exposure for 20 and 60min. Therefore, our results demonstrate that extremely low-frequency EFs alter stress response of mice in a kV/m- and duration-dependent manner. © 2015 Wiley Periodicals, Inc. Source

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