Hainan UniversityHaikou

Hainan, China

Hainan UniversityHaikou

Hainan, China
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Liao W.,Hainan UniversityHaikou | Liu Y.,Hainan UniversityHaikou | Xia G.,Hainan UniversityHaikou | Shen X.R.,Hainan UniversityHaikou | And 2 more authors.
Journal of Food Biochemistry | Year: 2017

In current study, three-spot seahorse (Hippocampus trimaculatus) skin collagen (SC) was extracted for the first time, and its physicochemical properties were characterized. Nile tilapia-skin collagen (TC) and pig-skin collagen (PC) were used to the references. The yields of SC and TC were 40.41±1.23 and 52.6±0.97% (dry weight), respectively. The SDS-PAGE and FTIR confirmed that SC, TC, and PC were preliminarily identified as type I collagen with triple helical structure. The denaturation temperatures of SC, TC, and PC were 35.12, 38.66, and 42.56°C, respectively. Gel-forming studies indicated SC exhibited higher capability than TC and PC under the effect of NaCl. After cross-linking by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), SC showed a significant increase in thermal stability and mechanical strength, and electron microscopic observation corroborated this phenomenon. Therefore, the isolated collagen from three-spot seahorse skin can be used in food packaging and biomaterials as alternatives to mammalian collagen. Practical applications: In this study, we evaluated a new source of skin collagen from three-spot seahorse which has been large-scale artificially cultured in recent years. The finding of this study demonstrate that SC exhibited higher gel-forming capability under the effect of NaCl, which suggest that it may be used in photographic film, sausage casings, and food additive as alternatives to mammalian collagen. Furthermore, thermal stability and mechanical strength of SC gel have a significant increase after cross-linking by EDC, so it can be further used for biomaterials, such as food packaging, drug delivery, tissue engineering constructs, and wound dressing systems. © 2017 Wiley Periodicals, Inc.


Wang S.,Hainan UniversityHaikou | Pang S.,Hainan UniversityHaikou | Xu N.,Hainan UniversityHaikou | Pan L.,Hainan UniversityHaikou | Lin Q.,Hainan Normal University
Journal of Applied Polymer Science | Year: 2016

In situ compatibilized poly(lactic acid)/thermoplastic polyester elastomer (PLA/TPEE) (80/20) blends are prepared by using multifunctional epoxide oligomer (coded as ADR) as a reactive modifier. Experiments such as torque, melt mass flow rate (MFR), SEM, DSC and tensile test were conducted to characterize properties of the PLA/TPEE/ADR blends. In-situ reactions between PLA, TPEE and ADR were researched using a lab torque rheometer. It was proposed that ADR may initiate a variety of chain extension/branching reactions between PLA and TPEE under mixing process. In particular, the formed copolymer PLA-ADR-TPEE could be viewed as an in-situ compatibilizer to improve the compatibility of PLA and TPEE. As expected, the value of MFR decreased greatly with increasing the ADR addition. The morphology reveals that interface adhesion of PLA/TPEE blend was enhanced with the incorporation of ADR, which led to a reduction in TPEE domain size. Moreover, tensile ductility of PLA/TPEE (80/20) blend was improved greatly by addition of the reactive modifier, e.g. the elongation at break was increased from 53% to the maximum value of 213% with addition of 1.2 phr ADR. The toughening effect can be explained by crazing with shear yielding mechanism. © 2016 Wiley Periodicals, Inc.


PubMed | Chinese Academy of Agricultural Sciences and Hainan UniversityHaikou
Type: | Journal: Frontiers in plant science | Year: 2016

To investigate unintended effects in genetically modified crops (GMCs), a comparative proteomic analysis between the leaves of the phytase-transgenic maize and the non-transgenic plants was performed using two-dimensional gel electrophoresis and mass spectrometry. A total of 57 differentially expressed proteins (DEPs) were successfully identified, which represents 44 unique proteins. Functional classification of the identified proteins showed that these DEPs were predominantly involved in carbohydrate transport and metabolism category, followed by post-translational modification. KEGG pathway analysis revealed that most of the DEPs participated in carbon fixation in photosynthesis. Among them, 15 proteins were found to show protein-protein interactions with each other, and these proteins were mainly participated in glycolysis and carbon fixation. Comparison of the changes in the protein and tanscript levels of the identified proteins showed that most proteins had a similar pattern of changes between proteins and transcripts. Our results suggested that although some significant differences were observed, the proteomic patterns were not substantially different between the leaves of the phytase-transgenic maize and the non-transgenic isogenic type. Moreover, none of the DEPs was identified as a new toxic protein or an allergenic protein. The differences between the leaf proteome might be attributed to both genetic modification and hybrid influence.


PubMed | Justus Liebig University, Chinese Academy of Agricultural Sciences and Hainan UniversityHaikou
Type: | Journal: Frontiers in plant science | Year: 2016

The Palmae family contains 202 genera and approximately 2800 species. Except for


PubMed | Chinese Academy of Agricultural Sciences and Hainan UniversityHaikou
Type: | Journal: Frontiers in plant science | Year: 2016

Reverse transcription quantitative real-time polymerase chain reaction (real-time PCR, also referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput method used to study gene expression. Despite the numerous advantages of RT-qPCR, its accuracy is strongly influenced by the stability of internal reference genes used for normalizations. To date, few studies on the identification of reference genes have been performed on cassava (Manihot esculenta Crantz). Therefore, we selected 26 candidate reference genes mainly via the three following channels: reference genes used in previous studies on cassava, the orthologs of the most stable Arabidopsis genes, and the sequences obtained from 32 cassava transcriptome sequence data. Then, we employed ABI 7900 HT and SYBR Green PCR mix to assess the expression of these genes in 21 materials obtained from various cassava samples under different developmental and environmental conditions. The stability of gene expression was analyzed using two statistical algorithms, namely geNorm and NormFinder. geNorm software suggests the combination of cassava4.1_017977 and cassava4.1_006391 as sufficient reference genes for major cassava samples, the union of cassava4.1_014335 and cassava4.1_006884 as best choice for drought stressed samples, and the association of cassava4.1_012496 and cassava4.1_006391 as optimal choice for normally grown samples. NormFinder software recommends cassava4.1_006884 or cassava4.1_006776 as superior reference for qPCR analysis of different materials and organs of drought stressed or normally grown cassava, respectively. Results provide an important resource for cassava reference genes under specific conditions. The limitations of these findings were also discussed. Furthermore, we suggested some strategies that may be used to select candidate reference genes.

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