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Chang S.-W.,Hainan Provincial Peoples Hospital
Journal of Practical Oncology

Objective: To investigate the clinical effect and side effects of paclitaxel combined with eapecitabine in the treatment for patients with advanced gastric cancer.Methods: Twenty patients with advanced gastric cancer recurrence after chemotherapy were treated with the combination of paclitaxel and capecitabine. The patients were administrated with oral capecitabine (1000 mg/m2, bid, d 1-14) and intravenous paclitaxel (135 mg/m2, d 1, d 8) in a 21-day cycle regimen.Results: A total of 5 cases showed PR (25.0%), 12 SD (60.0%) and 3 PD (15.0%). Meanwhile, the response rate (RR) was 25.0% and the disease control rate (DCR) was 85.0%. The median remission time and the median survival time were 5 months and 12 months, respectively. The main side effects were myelosuppression and hand-foot syndrome. The degree of these side reactions were only I or II level.Conclusion: The combination of paclitaxel and capecitabine is effective and well-tolerated in the treatment of advanced gastric cancer recurrence after chemotherapy. Source

Zhao Z.-Y.,Sun Yat Sen University | Luan P.,Shenzhen University | Huang S.-X.,Hainan Provincial Peoples Hospital | Xiao S.-H.,Sun Yat Sen University | And 5 more authors.
CNS Neuroscience and Therapeutics

Aims: Oxidative stress is frequently implicated in the pathology of neurodegenerative diseases. This study aimed to investigate the effects and their underlying mechanism(s) of edaravone upon hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in HT22 cells, a murine hippocampal neuronal model. Methods: HT22 cells were treated with H2O2 in the presence of various concentrations of edaravone or in its absence. A CCK-8 assay, Hoechst 33342 staining, and flow cytometry were used to detect cytotoxicity and apoptosis. In addition, the levels of reactive oxygen species (ROS) and the expression of Bcl-2, Bax, p-ERK 1/2, p-JNK, and p-P38 proteins in HT22 cells were examined. Results: Exogenous H2O2 decreased cell viability in a concentration-dependent manner and was associated with increased apoptosis and ROS production. Moreover, H2O2 significantly activated and upregulated the expression of p-ERK 1/2, p-JNK, and p-P38, while edaravon protected HT22 cells against H2O2-induced injury by inhibiting the production of ROS and activating the MAPK signaling pathway. Conclusions: Our results provide the first evidence that edaravone can protect H2O2-induced cell injury in HT22 neurons via its antioxidant action. These findings suggest that edaravone may be useful in the treatment of neurodegenerative disorders in which oxidative stress has been principally implicated. © 2012 Blackwell Publishing Ltd. Source

Chen P.,Chengdu Seventh Peoples Hospital | Zhu J.,University of Sichuan | Liu D.-Y.,Chengdu Seventh Peoples Hospital | Li H.-Y.,Hainan Provincial Peoples Hospital | And 2 more authors.
Medical Oncology

The expression of survivin, an inhibitor of apoptosis can be seen in most tumors and is correlated with the angiogenic factor vascular endothelial growth factor (VEGF). But little is known about their contribution in small-cell lung cancer (SCLC). This study was designed to investigate the expression of survivin and VEGF in SCLC, and to explore their correlation with clinical-pathological feature and prognosis. Forty-five patients with pathological histology of SCLC were entered into this study. Forty-five cases of matched adjacent non-tumor samples and 10 samples of operated patients with benign lung tumor were also included as control. The expression of survivin and VEGF was detected by immunohistochemistry (IHC, SP). These two sets of data were processed and tested for correlation with major patients' characteristics, and overall survival. The correlations between survivin and VEGF expressions and the clinical- pathological features were evaluated by chi-square test. The correlation between survivin and VEGF expressions was analyzed by Spearman's rank correlation test; the overall survival was analyzed by the Kaplan-Meier method; and the relationship between clinical and pathological features and overall survival was analyzed by the Cox proportional hazard models. Positive expression rate of survivin and VEGF was significantly higher in SCLC than those of adjacent non-tumor tissues and benign lung tumor tissues (73.3 vs. 15.6 vs. 0 %, P < 0.05) and (75.6 vs. 20 vs. 0 %, P < 0.05), respectively. Survivin and VEGF expressions were significantly associated with lymph node metastasis (P = 0.003, 0.011) and clinical stage (P = 0.006, 0.021). The expression of survivin was significantly coincident with the expression of VEGF (r = 0.644, P = 0.000). The median overall survival in survivin positive group and VEGF positive group was significantly shorter than those in survivin negative and VEGF negative group, respectively (log-rank P = 0.000). Moreover, multivariate analysis showed that survivin expression (HR 0.224; 95 % CI 0.074-0.675; P = 0.008) and VEGF expression (HR 0.172; 95 % CI 0.054-0.559; P = 0.003) were statistically independent predictive factors of poorer prognosis for SCLC patients. Our results indicated that survivin and VEGF were over-expressed in small-cell lung cancer, each of them may be an independent poor prognostic factor. © 2013 Springer Science+Business Media New York. Source

Yang B.,Chongqing Medical University | Zhou X.,Chongqing Medical University | Lan C.,Hainan Provincial Peoples Hospital
BMC Gastroenterology

Background: Irritable bowel syndrome (IBS) is a highly prevalent functional gastrointestinal disorder. Post-infectious IBS (PI-IBS) is caused by an acute gastrointestinal infection preceding the onset of symptoms. However, the pathophysiology of PI-IBS is not clear, and the purpose of this study was to investigate the probable immune mechanisms of PI-IBS. Methods: C57BL/6 mice were randomly assigned to either an infection group or a control group. Mice in the infection group were infected with Trichinella spiralis to establish a model of PI-IBS (500 Trichinella), while control mice received only salt solution. Visceral sensitivity of colorectal distention in mice was evaluated by abdominal withdrawal reflex scores and intestinal inflammation was assessed using hematoxylin-eosin staining; at day 56 post-infection, the mRNA and protein levels of specific cytokines in the gut segments were detected using reverse-transcription polymerase chain reaction and enzyme-linked immunoabsorbent assay. Results: Levels of interferon γ and interleukin (IL)-17 in the PI-IBS group were significantly increased in the duodenum and ileum, and IL-10 was decreased in the jejunum, ileum, and colon compared with control mice. However, the expression level of IL-1β was not significantly different between the two groups. Conclusions: The present study suggests that the local low-grade inflammation and immune activation that are an important component of the pathophysiology of PI-IBS are primarily induced and maintained by specific cytokines. © Yang et al.; licensee BioMed Central. Source

Yang B.,Chongqing Medical University | Zhou X.-C.,Chongqing Medical University | Lan C.,Hainan Provincial Peoples Hospital
Molecular Medicine Reports

The interstitial cells of Cajal (ICC) are basic components of gastrointestinal motility. However, changes in ICC and their role in post-infection irritable bowel syndrome (PI-IBS) remain to be elucidated. To observe the impact of alterations in the ICC on intestinal motility in a PI-IBS mouse model, female C57BL\6 mice were infected by the oral administration of 400 Trichinella spiralis larvae. The abdominal withdrawal reflex, intestine transportation time (ITT), grain numbers, Bristol scores, wet/dry weights and the percentage water content of the mice feces every 2 h were used to assess changes in the intestinal motor function. The intestines were excised and sectioned for pathological and histochemical examination. These intestines were also used to quantify the protein and mRNA expression of c-kit. The C57BL\6 mouse can act as a PI-IBS model at day 56 post-infection. Compared with the control mice, the ITT was shorter, the grain numbers, Bristol scores, wet weights and water contents of the mice feces were higher and the dry weights were unchanged in the PI-IBS mice. The protein and mRNA expression levels of c-kit were upregulated in the entire PI-IBS mouse intestines. Following immunohistochemical staining, the increased number of c-kit-positive cells were detected predominantly in the submucosa and myenteron. These results suggested that the alterations of the ICC resulted in the changes of the intestinal motility patterns in the PI-IBS mouse models induced by Trichinella spiralis infection, which may be the main mechanism underlying intestinal motility disorders in PI-IBS. Source

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