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Ding Y.-P.,Hainan Provincial Peoples Hospital | Yao H.-X.,Hainan Provincial Peoples Hospital | Tang X.-L.,Research Center for in Hainan Medical College | He H.-W.,Hainan Provincial Nongken Hospital | And 6 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2012

Objective: To investigate the prevalence and risk factors of bronchial asthma in the Li ethnic group in Hainan, China. Methods: This study employed a stratified random sample design using custom-designed questionnaires. Subjects with asthma-like symptoms were first identified by two rounds of surveys and then confirmed by respiratory physicians using pulmonary function test, bronchial dilation test and challenge test. Demographic data, information on family history of asthma, history of allergies, smoking habits, domestic cooking fuel and other potential risk factors were collected. Logistic regression was performed to evaluate risk factors for asthma. Results: The prevalence of asthma in the Li ethnic group was 3.38%, much higher than the national average level in China. Aging, agriculture industry (the rubber industry in particular), rural residence, family history of asthma, history of allergies, cold air, inhalation of dust and irritant gases, smoking, domestic cooking fuel and living environment were associated with increased risk of asthma. Conclusions: The high prevalence of asthma in the Li ethnic group highlights the importance of asthma prevention and treatment in this population. Risk factors indentified in this study warrant special attention. Elevating public awareness about asthma in local communities will benefit the prevention of the disease. © 2012 Hainan Medical College.


Huang F.,Sun Yat Sen University | Tang J.,Sun Yat Sen University | Zhuang X.,Hainan Provincial Nongken Hospital | Zhuang Y.,Sun Yat Sen University | And 4 more authors.
PLoS ONE | Year: 2014

Aberrant expression of miR-196a has been frequently reported in different cancers including pancreatic cancer. However, its function in pancreatic cancer has not been fully elucidated. Here, we investigated the expression pattern and the biological role of miR-196a in pancreatic cancer cell lines, as well as its interaction with a metastasis-related gene, nuclear factor-kappa-B-inhibitor alpha (NFKBIA). We demonstrated that miR-196a was up-regulated in human pancreatic cancer cell lines compared with immortalized pancreatic ductal epithelial cells by means of microRNAs microarray and qRT-PCR. Furthermore, down-regulation of miR-196a in PANC-1 suppressed its proliferation and migration with an increase in G0/G1 transition and decreased expression of Cyclin D1 and CDK4/6. Meanwhile, an increased expression in E-cadherin and decreased expression in N-cadherin and Vimentin were also observed. We identified a novel miR-196a target, NFKBIA, and down-regulation of miR-196a enhanced the expression of NFKBIA protein. Luciferase assay confirmed that NFKBIA was a direct and specific target of miR-196a. Silencing NFKBIA in PANC-1 cells enhanced its proliferation and migration. Taken together, our findings indicate that miR-196a is highly expressed in pancreatic cancer cell lines, and may play a crucial role in pancreatic cancer proliferation and migration, possibly through its downstream target, NFKBIA. Thus, miR-196a may serve as a potential therapeutic target for pancreatic cancer. © 2014 Huang et al.


Chen W.-B.,Sun Yat Sen University | Huang F.-T.,Sun Yat Sen University | Zhuang Y.-Y.,Sun Yat Sen University | Tang J.,Sun Yat Sen University | And 4 more authors.
Digestive Diseases and Sciences | Year: 2013

Background/Aims: Pancreatic cancer has the worst prognosis of any gastrointestinal cancer with a mortality rate approaching its incidence. Previous studies have indicated that GATA6 plays a key role in organ development and function, and that abnormal expression of GATA6 may induce tumorigenesis. Meanwhile, it has been reported that generation of reactive oxygen species contributes to carcinogenesis. In this study, we set out to study the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species. Methods: Four target miRNA sequences against GATA6 mRNA were synthesized and used to transfect SW1990 cells. Then, GATA6 expression in SW1990 cells was examined by western blot and quantative real-time polymerase chain reaction. Cell proliferation was examined by WST-8 and colony formation assay. Cell cycle progression and apoptosis were measured by flow cytometry. We also measured the generation of reactive oxygen species by immunofluorescence and flow cytometry. Results: RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. Silencing of GATA6 by RNA interference inhibited cell proliferation and increased apoptosis of SW1990, and enhanced the expression of reactive oxygen species. Conclusions: These results suggest that the RNA interference approach against GATA6 may be an effective therapeutic approach for treatment of pancreatic cancer. © 2013 Springer Science+Business Media New York.


Sun Y.-C.,Sun Yat Sen University | Sun Y.-C.,Guangdong Medical College | Sun Y.-C.,Hainan Provincial Nongken Hospital | Wang J.,Sun Yat Sen University | And 10 more authors.
BMC Cancer | Year: 2014

Background: Although the incidence of glioma is relatively low, it is the most malignant tumor of the central nervous system. The prognosis of high-grade glioma patient is very poor due to the difficulties in complete resection and resistance to radio-/chemotherapy. Therefore, it is worth investigating the molecular mechanisms involved in glioma drug resistance. MicroRNAs have been found to play important roles in tumor progression and drug resistance. Our previous work showed that miR-181b is involved in the regulation of temozolomide resistance. In the current study, we investigated whether miR-181b also plays a role in antagonizing the effect of teniposide.Methods: MiR-181b expression was measured in 90 glioma patient tissues and its relationship to prognosis of these patients was analyzed. Cell sensitivity to teniposide was tested in 48 primary cultured glioma samples. Then miR-181b stably overexpressed U87 cells were generated. The candidate genes of miR-181b from our previous study were reanalyzed, and the interaction between miR-181b and target gene MDM2 was confirmed by dual luciferase assay. Cell sensitivity to teniposide was detected on miR-181b over expressed and MDM2 down regulated cells.Results: Our data confirmed the low expression levels of miR-181b in high-grade glioma tissues, which is related to teniposide resistance in primary cultured glioma cells. Overexpression of miR-181b increased glioma cell sensitivity to teniposide. Through target gene prediction, we found that MDM2 is a candidate target of miR-181b. MDM2 knockdown mimicked the sensitization effect of miR-181b. Further study revealed that miR-181b binds to the 3'-UTR region of MDM2 leading to the decrease in MDM2 levels and subsequent increase in teniposide sensitivity. Partial restoration of MDM2 attenuated the sensitivity enhancement by miR-181b.Conclusions: MiR-181b is an important positive regulator on glioma cell sensitivity to teniposide. It confers glioma cell sensitivity to teniposide through binding to the 3'-UTR region of MDM2 leading to its reduced expression. Our findings not only reveal the novel mechanism involved in teniposide resistance, but also shed light on the optimization of glioma treatment in the future. © 2014 Sun et al.; licensee BioMed Central Ltd.


PubMed | Hainan Provincial Nongken Hospital and Sun Yat Sen University
Type: Journal Article | Journal: PloS one | Year: 2014

Aberrant expression of miR-196a has been frequently reported in different cancers including pancreatic cancer. However, its function in pancreatic cancer has not been fully elucidated. Here, we investigated the expression pattern and the biological role of miR-196a in pancreatic cancer cell lines, as well as its interaction with a metastasis-related gene, nuclear factor-kappa-B-inhibitor alpha (NFKBIA). We demonstrated that miR-196a was up-regulated in human pancreatic cancer cell lines compared with immortalized pancreatic ductal epithelial cells by means of microRNAs microarray and qRT-PCR. Furthermore, down-regulation of miR-196a in PANC-1 suppressed its proliferation and migration with an increase in G0/G1 transition and decreased expression of Cyclin D1 and CDK4/6. Meanwhile, an increased expression in E-cadherin and decreased expression in N-cadherin and Vimentin were also observed. We identified a novel miR-196a target, NFKBIA, and down-regulation of miR-196a enhanced the expression of NFKBIA protein. Luciferase assay confirmed that NFKBIA was a direct and specific target of miR-196a. Silencing NFKBIA in PANC-1 cells enhanced its proliferation and migration. Taken together, our findings indicate that miR-196a is highly expressed in pancreatic cancer cell lines, and may play a crucial role in pancreatic cancer proliferation and migration, possibly through its downstream target, NFKBIA. Thus, miR-196a may serve as a potential therapeutic target for pancreatic cancer.


Wu J.,Wuhan University | Wu J.,Hainan Provincial Nongken Hospital | Cheng G.,Wuhan University | Lu Z.,Wuhan University | And 3 more authors.
Biological Trace Element Research | Year: 2015

The objective of this study is to investigate the impacts of Methyl Mercury Chloride (MMC) on cognitive functions and ultrastructural changes of hippocampus in Sprague Dawley (SD) rats. Thirty healthy 20-day-old male SD rats weighing 30–40 g were randomly divided into three groups to receive daily injections. Two different dose levels were used: 4 mg/kg as high dose (H-MMC) and 2 mg/kg as low dose (L-MMC).The control group received 4 mg/kg saline solution (N-NaCl). After daily subcutaneous injection for 50 days, 6-day Morris water maze tests were used to assess the learning and memory functions of the rats. After a 5-day continuous training, spatial probe tests were conducted of times and paths crossing to the target quadrant on the 6th day. After the rats were euthanized, their hippocampus sections were stained with hematoxylin and eosin and analyzed under bothoptical microscope and electron microscope. The time H-MMC group spent in finding platform was significantly longer as compared toN-NaCl group on day 2 to day 5 and L-MMC group on day 4 to day 5. The number of crossing times of H-MMC group to the target quadrant was 0.63 ± 0.74, which is much lower than C-NaCl group (3.13 ± 1.56) with P value <0.05. No statistically significant difference in crossing times was found between L-MMC and C-NaCl groups. For H-MMC group, decreasing number of neurons and disorganized nerve cells were examined under light microscope. Swelling and dissolution of Golgi complex were examined under electron microscope, along with endoplasmic reticulum expansion and cytoplasmic edema. Mild cytoplasmic edema was found in L-MMC group. MMC can cause cognitive impairment in terms of learning and memory in SD rats. Additionally, it can also cause changes in the ultrastructure of neurons and morphological changes in the hippocampus, causing significant damage. © 2015 The Author(s)


Su Q.,Hainan Provincial Nongken Hospital | Yuan K.,Hainan Provincial Nongken Hospital | Long F.,Hainan Provincial Nongken Hospital | Wan Z.,Hainan Provincial Nongken Hospital | And 13 more authors.
Vascular | Year: 2014

Survivors of ischemic stroke are still at a significant risk for recurrence. Numerous effective strategies for the secondary prevention of ischemic stroke have now been established; however, these guidelines are not widely known. In this retrospective, a multicenter study was conducted from January 2011 to February 2012 in 10 general hospitals, which included 1300 elderly patients who had previously been diagnosed with ischemic stroke and re-admitted to hospitals. Logistic regression models were fitted to determine the relationship between compliance with secondary prevention therapy and each variable of interest. The treatment rates of antihypertensive, antiplatelet and lipid-lowering therapy were only 56.3%, 48.9% and 19.6%, respectively. Multivariate analysis presented that cardiovascular risk factors would motivate patients with hypertension and hyperlipidemia to receive corresponding treatments. However, it is worth noting that they did not influence the use of antiplatelet therapy. In addition, high education, health education and insurance promote the use of secondary prevention in patients. In conclusion, the importance of antiplatelet therapy should not be ignored any more. Besides, health education will raise patients' attention to ischemic stroke. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.


Nie Z.-S.,Hainan Provincial Nongken Hospital | Li C.-Z.,Hainan Provincial Nongken Hospital | Xiao J.-Z.,Hainan Provincial Nongken Hospital | Che X.-P.,Hainan Provincial Nongken Hospital | Zhang G.-Q.,Hainan Provincial Nongken Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: In recent years, some researchers began to apply a relatively non-invasive multi-slice spiral computerized tomography (CT) to replace digital subtraction angiography (DSA) to evaluate renal anatomy and function of kidney transplantation, but the relevant reports mainly focused on that in the 16-slice (or below) spiral CT, few on the 64-slice spiral CT. OBJECTIVE: To evaluate the value of 64-slice spiral CT three-dimensional reconstruction in donor and recipient kidney for renal transplantation. METHODS: Seven patients who were voluntary to donate their one kidney to their relatives and 6 patients who were about to receive kidney from others were done plain CT scan, arterial phase, venous phase and delayed phase enhanced scanning, and enhanced scanning periods were processed with three-dimensional reconstruction techniques including multi planar reconstruction, volume rendering technique, maximumintensity projection and curved projection reconstruction. RESULTS AND CONCLUSION: In 7 donors, according to CT angiography, one renal artery in each side of the kidneys was presented in 4 cases, two renal arteries in the left kidney and one in the right in 1 case, three renal arteries in the left kidney and two renal arteries in the right in 2 cases; one renal vein and one set of renal pelvis and ureter were presented in both left and right kidney in all cases. MPR reconstruction showed left renal cyst in 1 case, kidney functions were normal in all cases according to CT urography. The above mentioned results shown by 64-slice spiral CT three-dimensional reconstruction were all proven by surgery. In recipients, according to CT angiography, renal artery, vein and ureter were no stricture and fistula in all 6 cases. CT scanning of 4 cases of the transplanted kidneys showed normal size, normal shape and density. Arterial phase enhanced scanning showed clear boundaries in renal cortex and renal medulla, and CT values were greater than 150 HU. Venous phase enhanced scanning showed the renal parenchyma uniform, CT urography showed urinary function normal. Artery enhanced scanning of 2 cases of the transplanted kidney showed unclear boundaries in renal cortex and renal medulla, CT values less than 100 HU. Venous phase enhanced scanning showed the kidney parenchyma were weak, delayed 10-minute scanning showed only a small amount of contrast medium excretion, which confirmed rejection by renal biopsy. 64 slice spiral CT three dimensional reconstruction can basically replace other imaging examinations before and after renal transplantation, and can be the best option for renal transplantation.


Yang W.-Z.,Hainan Provincial Nongken Hospital | Wen B.-S.,Hainan Provincial Nongken Hospital | Lai X.-Z.,Hainan Provincial Nongken Hospital | Pang H.-L.,Hainan Provincial Nongken Hospital | And 2 more authors.
World Chinese Journal of Digestology | Year: 2010

AIM: To evaluate the feasibility, efficacy and safety of endoscopic ultrasound-assisted endoscopic submucosal resection (ESR) of tumors originating from the muscularis propria of the esophagus. METHODS: Twelve patients with esophageal submucosal tumors revealed by gastroscopy were included in this study. Endoscopic ultrasound revealed that all tumors were located in the muscularis propria of the esophagus. Endoscopic submucosal resection was performed in all patients. The entire operation was performed under general anesthesia in the operating room. Gastroscopy was performed again one and three months postoperatively. RESULTS: The operation duration ranged from 90 to 150 min, with an average value of 100 min. The size of tumors ranged from 1.1 to 2.2 cm, with an average value of 1.5 cm. All the tumors were resected en bloc. Postoperative pathological examination and immunohistochemical staining revealed leiomyoma in 11 patients and interstitialoma in one patient. CONCLUSION: Endoscopic submucosal resection is safe and effective in the management of tumors originating from the muscularis propria of the esophagus.

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