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Song W.,Hainan Provincial Nongken General Hospital | Wu L.,Hainan Provincial Nongken General Hospital | Zhao H.,Hainan Provincial Nongken General Hospital
Journal of Practical Oncology | Year: 2017

Objective: To analyze the clinical value of carbohydrate antigen 19-9 (CA19-9), CA242 and carcinoembryonic antigen (CEA) combined detection in the diagnosis and clinical staging of pancreatic cancer. Methods: Ninety-five cases of pancreatic cancer patients were selected as the patient group, and 60 cases of healthy subjects were enrolled as the control group. The expression levels of serum CA19-9, CA242, and CEA were compared between the two groups. The sensitivity, specificity and accuracy of serum CA19-9, CA242 and CEA detection alone and combined detection in the diagnosis of pancreatic cancer were analyzed. Besides, the serum levels of the above biomarkers were compared in pancreatic cancer patients of different clinical stages. The role of these antigens in the clinical staging of pancreatic cancer was further evaluated. Results: The serum levels of CA19-9, CA242 and CEA in the patients were significantly higher than those in the control group (P<0.05). The sensitivity, specificity and accuracy of biomarkers combined detection in diagnosing pancreatic cancer were 96.8%, 66.7% and 85.2%, respectively, and both the sensitivity and accuracy were higher than those of a single test. As the pathological stages of pancreatic cancer patients increased, the serum levels of CA19-9, CA242 and CEA were all significantly increased (P<0.05). Moreover, at 6 months after treatment, the serum levels of CA19-9, CA242 and CEA were significantly decreased, compared with those before treatment (P<0.05). Furthermore, patients with tumors ≥5 cm in diameter, or tumors located in the pancreatic body or tail, showed significantly higher levels of serum CA19-9, CA242 and CEA, compared to those patients with tumors <5 cm in diameter or tumors in the pancreatic head or the entire pancreas (P<0.05). Conclusion: The combined detection of serum CA19-9, CA242 and CEA has important clinical value in the early diagnosis and staging of pancreatic cancer. © 2017, The Second Affiliated Hospital, College of Medicine, Zhejiang University. All right reserved.


PubMed | Nanjing Potomac Biotechnology Co., Hainan University and Hainan Provincial Nongken General Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2014

We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv genomic DNA were amplified by polymerase chain reaction, inserted into the expression vector pET-30a, and expressed in Escherichia coli. An enzyme-linked immunosorbent assay was used to detect the 4 M. tuberculosis antibodies. Engineered E. coli bacteria expressing TB16.3 and the 3 other proteins were constructed and found mainly to be soluble. For recombinant TB16.3 proteins, serum samples of 118 tuberculosis (TB) patients and 96 healthy controls were analyzed. Sensitivity, specificity, and adjusted concordance rate for the TB16.3 antibody were 72.9, 86.5, and 79.6%, respectively. The positive rate of Rv2626C antibody in TB patients (44.1%) was significantly lower than that in normal controls (75.0%, (2) = 20.8, P < 0.01). TB15.3 and TB16.3 were used for simultaneous detection and showed sensitivity, specificity, and repeatability rates of 69.4, 96.9, and 83.7%. The antibody positive rate and specificity for patients with lung disease was 9.6 and 90.4%, respectively. TB15.3 and TB16.3 were mixed and detected simultaneously. Combined with the results for TB15.3, the sensitivity, specificity, and concordance rates were 82.2, 95.9, and 88.9%, respectively. The concordance rate was the highest value observed. Target genes were cloned into a host strain and expressed successfully. The TB16.3 recombinant protein may be used as a new serological antigen for tuberculosis diagnosis.


Wang X.,Zhengzhou University | Hao J.,Hainan Provincial Nongken General Hospital | Zhang F.,Zhengzhou University | Li J.,Zhengzhou University | And 2 more authors.
Fertility and Sterility | Year: 2016

Objective: To investigate the combined effects of Chinese couples' body mass indices (BMIs) on the outcomes of IVF or intracytoplasmic sperm injection (ICSI) and the clinical characteristics of their neonates. Design: Retrospective cohort study. Setting: University-affiliated reproductive medicine center. Patient(s): A total of 12,061 first fresh IVF/ICSI cycles with autologous oocytes from September 1, 2009 to December 31, 2014. Intervention(s): None. Main Outcome Measure(s): The primary treatment outcome was live birth, and the primary outcome for the neonates was neonatal birth weight (NBW). Result(s): After adjusting for confounders, our study showed that in IVF cycles, couples with a female BMI ≥25 kg/m2 had a significantly higher odds of abortion and a significantly lower odds of live birth than couples with both male and female BMIs <25 kg/m2. Regarding ICSI cycles, no significant relationship was found between the couples' BMIs and the live birth rate. The NBWs of singletons conceived via IVF/ICSI cycles was significantly higher when their parents' BMIs were greater; however, no significant differences were observed in the NBWs of twins conceived via IVF/ICSI cycles. Conclusion(s): Increased female BMI negatively affected live births conceived via IVF. Regarding ICSI, no significant differences were found in the outcomes in terms of parental BMI. The singleton neonates' NBWs increased with parental BMI conceived via IVF/ICSI. However, parental BMI did not significantly affect the NBWs of twins conceived via IVF/ICSI. © 2016 American Society for Reproductive Medicine.


Hu X.,Hainan Provincial Nongken General Hospital | Che X.,Hainan Provincial Nongken General Hospital | Xiao J.,Hainan Provincial Nongken General Hospital | Wang Z.,Huazhong University of Science and Technology | Zhu H.,Case Western Reserve University
International Journal of Clinical and Experimental Medicine | Year: 2016

Primitive neuroectodermal tumor in adults is extremely rare. It belongs to the Ewing’s sarcoma family that usually occurs in children and adolescents with a predilection for the truncal and axial soft tissue. Primitive neuroectodermal tumor of the posterior urethra is even less common with no prior reported cases in the English-language literature. We reported here a case of primitive neuroectodermal tumor of the posterior urethra with local bladder wall invasion and distant pulmonary metastasis in a 49-year-old man. The patient’s treatment and clinical outcome were examined with literature review for the management of primitive neuroectodermal tumor in general with emphasis on its management in the genitourinary tract. © 2016, E-Century Publishing Corporation. All rights reserved.


Yao Z.J.,Hainan Provincial Nongken General Hospital
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To investigate the malignancy incidence among rubber manufacture and preliminary processing industrial workers in Hainan Province of China. A retrospective survey was conducted on 9 farms in Hainan covering workers who got malignant tumor between January 1, 2005 and October 31, 2010. On average there were 10 320 workers worked on the 9 farms each year. Among them, 167 cases of malignancy were found. The morbidity was 279.01 every a hundred thousand and standardized morbidity was 159.03 every a hundred thousand; In comparison, we also investigated 14228 non-rubber-industry-related workers. 94 malignancy cases were found. The morbidity was 113.91 every a hundred thousand and standardized morbidity was 64.93 every a hundred thousand. The difference was statistically significant (P<0.01) meaning that rubber industrial workers in Hainan Province had a higher possibility of getting malignancy. Top 5 types of cancer found in this study were lung cancer (17.96%), liver cancer (17.36%), stomach cancer (13.17%), rectal cancer and nasopharyngeal carcinoma. 116 cases of these five types of cancer took up 69.45% of the total. Rubber industrial workers in Hainan have a higher morbidity of malignancy than other local industries. But it is similar to other areas in China.


Wang B.-P.,Hainan Provincial Nongken General Hospital | Chen H.-C.,Hainan Provincial Nongken General Hospital
World Chinese Journal of Digestology | Year: 2015

Aim: To investigate the diagnostic value of mesenteric computed tomography (CT) angiography in elderly patients with primary small intestinal space-occupying lesions.Methods: A retrospective analysis was performed of 100 elderly patients with primary small intestinal lesions who underwent mesenteric CT angiography. Two radiologists localized and diagnosed the lesions using artery location method and Cole’s method. The diagnostic accuracy was compared between different methods and radiologists.Results: Of the 100 cases of small intestinal lesions detected by CT angiography, 28 showed arterial enlargement, 21 showed venous enlargement and tortuosity, 29 produced tumor vessels, and 47 had enlargement and thickening of the vasa recta on the mesenteric side. The accuracy of artery location method and Cole’s method was 99% and 85%, respectively, and both were accurate in venous localization in the duodenum and ileocecal regions. The accuracy of the artery location method was relatively low in the ileum and jejunum (76.6% and 55.6%). Two radiologists had high consistency (Kappa = 1.00, P = 0.000) when using the artery location method, and moderate consistency (Kappa = 0.54, P = 0.000) when using the Coles method. There was no significant difference in localization accuracy between the two physicians (P > 0.05).Conclusion: Mesenteric CT angiography can clearly show the blood supplying vessels of primary small intestinal lesions and therefore contribute to the localization and qualitative diagnosis of these lesions. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Ou-Yang Y.,Hainan Provincial Nongken General Hospital | Wang G.-M.,Hainan Provincial Nongken General Hospital
World Chinese Journal of Digestology | Year: 2015

AIM: To assess the effect of short- vs long-term non-lactose milk powder feeding on neonatal diarrhea. METHODS: Eighty infants with diarrhea were included and divided into an observation group and a control group. The observation group was given short-term non-lactose milk powder feeding, and the control group was given long-term non-lactose milk powder feeding. The gastrointestinal tolerance and weight gain were recorded and compared between the two groups. RESULTS: The total effectiveness in the observation group was higher than that in the control group, although there was no statistically significant difference (97.5% vs 95.0%, P > 0.05). Stool frequency and the percentages of patients with vomiting and abdominal distension were significantly lower in the observation group than in the control group (P < 0.05). The observation group had significantly greater total body fat (TBF) and percentage of body fat (F%) but significantly decreased lean body mass (LBM) vs the control group (P < 0.05). CONCLUSION: Short-term non-lactose milk powder feeding can reduce the incidence of gastrointestinal symptoms and conforms to the infant growth characteristics. © 2015 Baishideng Publishing Group Inc. All rights reserved.


PubMed | Zhengzhou University and Hainan Provincial Nongken General Hospital
Type: Journal Article | Journal: Fertility and sterility | Year: 2016

To investigate the combined effects of Chinese couples body mass indices (BMIs) on the outcomes of IVF or intracytoplasmic sperm injection (ICSI) and the clinical characteristics of their neonates.Retrospective cohort study.University-affiliated reproductive medicine center.A total of 12,061 first fresh IVF/ICSI cycles with autologous oocytes from September 1, 2009 to December 31,2014.None.The primary treatment outcome was live birth, and the primary outcome for the neonates was neonatal birth weight (NBW).After adjusting for confounders, our study showed that in IVF cycles, couples with a female BMI 25kg/m(2) had a significantly higher odds of abortion and a significantly lower odds of live birth than couples with both male and female BMIs <25kg/m(2). Regarding ICSI cycles, no significant relationship was found between the couples BMIs and the live birth rate. The NBWs of singletons conceived via IVF/ICSI cycles was significantly higher when their parents BMIs were greater; however, no significant differences were observed in the NBWs of twins conceived via IVF/ICSI cycles.Increased female BMI negatively affected live births conceived via IVF. Regarding ICSI, no significant differences werefound in the outcomes in terms of parental BMI. The singleton neonates NBWs increased with parental BMI conceived via IVF/ICSI. However, parental BMI did not significantly affect the NBWs of twins conceived via IVF/ICSI.


PubMed | Shenyang University, Hainan Medical University, Hainan Provincial Nongken General Hospital and Shenyang Medical College
Type: | Journal: Brain research | Year: 2015

Endophilin-1 (Endo1), a multifunctional protein, is essential for synaptic vesicle endocytosis. However, the role and mechanism of endophilin-1 in blood-brain barrier (BBB) function are still unclear. This study was performed to determine whether endophilin-1 regulated BBB permeability via the EGFR-JNK signaling pathway. In the present study, we found that endophilin-1 over-expression in human cerebral microvascular endothelial cell (hCMEC/D3) increased BBB permeability and meanwhile reduced the expression levels of epidermal growth factor receptor (EGFR), phosphorylated c-Jun N-terminal kinase (p-JNK). While endophilin-1 knockdown led to the contrary results. After JNK inhibitor SP600125 was administered to the endophilin-1 silenced hCMEC/D3 cells, the transendothelial electrical resistance (TEER) value was decreased and the permeability coefficient values to 4kDa and 40kDa FITC-dextran were increased. Results observed by Transmission electron microscopy (TEM) showed that tight junctions (TJs) were opened. Moreover, immunofluorescence and Western blot assays revealed the discontinuous distribution of TJ-associated proteins ZO-1, occludin on cell-cell boundaries and a significant decrease in protein expressing levels. Therefore, these results indicated that endophilin-1 positively regulated BBB permeability via the EGFR-JNK signaling pathway in hCMEC/D3 cells, which would provide an experimental basis for further research on endophilin-1 mediated the opening of BBB.


PubMed | Hainan Provincial Nongken General Hospital
Type: Journal Article | Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2012

To investigate the malignancy incidence among rubber manufacture and preliminary processing industrial workers in Hainan Province of China.A retrospective survey was conducted on 9 farms in Hainan covering workers who got malignant tumor between January 1, 2005 and October 31, 2010.On average there were 10 320 workers worked on the 9 farms each year. Among them, 167 cases of malignancy were found. The morbidity was 279.01 every a hundred thousand and standardized morbidity was 159.03 every a hundred thousand; In comparison, we also investigated 14228 non-rubber-industry-related workers. 94 malignancy cases were found. The morbidity was 113.91 every a hundred thousand and standardized morbidity was 64.93 every a hundred thousand. The difference was statistically significant (P<0.01) meaning that rubber industrial workers in Hainan Province had a higher possibility of getting malignancy. Top 5 types of cancer found in this study were lung cancer (17.96%), liver cancer (17.36%), stomach cancer (13.17%), rectal cancer and nasopharyngeal carcinoma. 116 cases of these five types of cancer took up 69.45% of the total.Rubber industrial workers in Hainan have a higher morbidity of malignancy than other local industries. But it is similar to other areas in China.

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