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Zhang X.-P.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | Zhang X.-P.,Shenyang Pharmaceutical University | Pei Y.-H.,Shenyang Pharmaceutical University | Liu M.-S.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | And 5 more authors.
Chinese Traditional and Herbal Drugs | Year: 2010

Objective: To study the organic acids in the leaves of Cerbera manghas. Methods: The constituents were isolated by repeated column chromatography and their structures were elucidated on basis of physico-chemical constants and spectral data analyses. Results: Seven compounds were obtained and determined to be m-carboxyphenylacetic acid (1), p-hydroxybenzoic acid (2), protocatechuic acid (3), p-hydroxycin-namic acid (4), isophthalic acid (5), vanillic acid (6), and succinic acid (7). Conclusion: Compound 1 is a new natural product, compounds 2-6 are obtained from the plants of Cerbera L. for the first time.

Yuan Y.,Chengdu University of Traditional Chinese Medicine | Yuan Y.,Hainan Normal University | Tan Y.-F.,Hainan Medical University | Tan Y.-F.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | And 18 more authors.
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2014

Background: Alpinia oxyphylla (Zingiberaceae), an herbaceous perennial plant, its capsular fruit is commonly used in traditional Chinese medicine for the treatment of different urinary incontinence symptoms including frequency, urgency and nocturia. These symptoms are similar to the overactive bladder syndrome. In our lab, we found that the 95% ethanol extract of the capsular fruits exhibited significant anti-muscarinic activity. Some constituents in capsular fruits including flavonoids (e.g., izalpinin and tectochrysin), diarylheptanoids (e.g., yakuchinone A and yakuchinone B) and sesquiterpenes (e.g., nootkatone), are regarded as representative chemicals with putative pharmacological activities.Objective: This study aimed to evaluate the in vitro antagonistic actions of izalpinin on carbachol-induced contraction of the rat detrusor muscle. Materials and Methods: In vitro inhibition of rat detrusor contractile response to carbachol was used to study the functional activity of izalpinin. The isolated detrusor strips of rats were mounted in organ baths containing oxygenated Krebs’ solution. The cumulative consecutive concentration-response curves to carbachol-evoked contractions in strips of rat bladder were obtained. Results: Carbachol induced concentration-dependent contractions of isolated rat bladder detrusor strips. The vehicle DMSO had no impact on the contraction response. The contraction effects were concentration-dependently antagonized by izalpinin, with a mean EC50 value of 0.35 µM. The corresponding cumulative agonist concentration-response curves shifted right-ward. Conclusions: Izalpinin exhibits inhibitory role of muscarinic receptor-related detrusor contractile activity, and it may be a promising lead compound to treat overactive bladder. © 2014, African Ethnomedicines Network. All rights reserved.

Chen F.,Hainan Medical University | Chen F.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | Li H.-L.,Hainan Medical University | Li H.-L.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | And 13 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2014

Alpinia oxyphylla (Yizhi) capsularfruits are commonly used in traditional medicine. Pharmacological studies have demonstrated that A. oxyphylla capsularfruits have some beneficial roles. Besides volatile oil, sesquiterpenes, diarylheptanoids and flavonoids are main bioactive constituents occurring in the Yizhi capsularfruits. The representative constituents include tectochrysin, izalpinin, chrysin, apigenin-4',7-dimethylether, kaempferide, yakuchinone A, yakuchinone B, oxyphyllacinol and nootkatone. Their content levels in the fruit and its pharmaceutical preparations have been reported by our group. The nine phytochemicals are also the major components present in the Yizhi alcoholic extracts, which have anti-diarrheal activities. However, the fates of these constituents in the body after oral or intravenous administration remain largely unknown. In the present study, we focus on these phytochemicals albeit other concomitant compounds. The chemicals and their metabolites in rat plasma were identified using liquid chromatography/tandem mass spectrometry with selected reaction monitoring mode after orally administered Yizhi extract to rats. Rat plasma samples were treated by methanol precipitation, acidic or enzymatic hydrolysis. This target analysis study revealed that: (1) low or trace plasma levels of parent chemicals were measured after p.o. administration of Yizhi extract, Suoquan capsules and pills to rats; (2) flavonoids and diarylheptanoids formed mainly monoglucuronide metabolites; however, diglucuronide metabolites for chrysin, izalpinin and kaempferide were also detected; (3) metabolic reduction of Yizhi diarylheptanoids occurred in rats. Yakuchinone B was reduced to yakuchinone A and then to oxyphyllacinol in a stepwise manner and subsequently glucuronidated by UDP-glucuronosyl transferase. Further research is needed to characterize the UDP-glucuronosyl transferase and reductase involved in the biotransformation of Yizhi chemicals. © 2014 Elsevier B.V.

Liu M.-S.,Hainan Medical University | Liu M.-S.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | Liu C.,Shenyang Pharmaceutical University | Zhang X.-P.,Hainan Medical University | And 6 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2010

Two new drimane sesquiterpenoids, changweikangic acid A (1) and B (2), were isolated from Compound Changweikang. Their structures were established on basis of extensive spectroscopic analyses including two dimensional (2D) NMR and X-ray crystallographic data. The two compounds were evaluated for their inhibitory activity against nitric oxide production in lipopolysaccharide- activated macrophage cell line, RAW 264.7 cells. © 2010 Pharmaceutical Society of Japan.

Liu M.,Hainan Medical University | Liu M.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | Kang S.,Hainan Medical University | Kang S.,Hainan Provincial Key Laboratory of R and D of Tropical Herbs | And 3 more authors.
Natural Product Research | Year: 2010

A new arbutin derivative, 6'-(2,5-dihydroxycinnamoyl) arbutin (1), was isolated from the n-BuOH extract of leaves of Heliciopsis lobata (Merr.) Sleum. Its structure was characterised by extensive spectrometric methods, including 1-D and 2-D NMR experiments. © 2010 Taylor & Francis.

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