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Lan C.,Hainan Provincial General Hospital | Sun X.-N.,Hainan Provincial General Hospital | Zhou X.-C.,Hainan Provincial General Hospital | Yang B.,Chongqing Medical University | And 4 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2016

Objective: To investigate the impact of the preinduced intestinal heat shock protein 70 (HSP70) on the visceral hypersensitivity and abnormal intestinal motility in a post-infectious irritable bowel syndrome (PI-IBS) mouse model. Methods: Eighty-four female C57BL/6 mice were randomly assigned to four groups: control group (n = 21) and induction + PI-IBS group (n = 21), PI-IBS group (n = 21) and induction group (n = 21). The mice in PI-IBS group were infected in vivo with Trichinella spiralis by oral administration. The visceral hypersensitivity and intestinal motility were evaluated respectively with abdominal withdrawal reflex and colon transportation test. The intestinal HSP70 protein and mRNA level was measured by Western blot and real-time PCR. Meanwhile, the intestinal proinflammatory cytokines IL-10 and TNF-α level was detected by ELISA. Results: Compared with their counterparts in PI-IBS group, the animals in the Induction + PI-IBS group show significantly increased intestinal level of HSP70 and obviously ameliorative clinical figures, including abdominal withdrawal reflex score, intestine transportation time and Bristol scores (P < 0.05). Meanwhile, the intestinal post-inflammatory cytokines remarkably changed, including increased IL-10 level and decreased TNF-α level (P < 0.05). Conclusions: Intestinal HSP70 may play a potential protective role through improving the imbalance between the intestinal post-inflammatory and anti-inflammatory cytokines in PI-IBS. © 2016 Hainan Medical College.

Zhou X.,Chongqing Medical University | Dong L.,Hainan Provincial General Hospital | Yang B.,Chongqing Medical University | He Z.,Hainan Provincial General Hospital | And 4 more authors.
Amino Acids | Year: 2015

This study aimed to investigate the protective effects of preinduction of heat shock protein 70 (HSP70) on Trichinella spiralis infection-induced post-infectious irritable bowel syndrome (PI-IBS) in mice. Trichinella spiralis infection significantly reduced HSP70 abundance, ileal villus height and crypt depth, expression of tight junctions, serum lysine and arginine concentrations, and ileal SCL7A6 and SCL7A7 mRNA levels, induced inflammatory response, and activated NF-κB signaling pathway. Meanwhile, the heat treatment upregulated HSP70 expression, and then reversed intestinal dysfunction and inflammatory response. Preinduction of HSP70 enhanced serum arginine and intestinal SCL7A7 expression and inhibited NF-κB activation compared with PI-IBS model. Treatment with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and N-nitro-l-arginine methyl ester hydrochloride (l-NAME, a nitric oxide synthase inhibitor, NOS) further demonstrated that preinduction of HSP70 might inhibit NF-κB and activated NOS/nitric oxide (NO) signaling pathways. In conclusion, preinduction of HSP70 by heat treatment may confer beneficial effects on Trichinella spiralis infection-induced PI-IBS in mice, and the protective effect of HSP70 may be associated with inhibition of NF-κB and stimulation of NOS/NO signaling pathways. © 2015 Springer-Verlag Wien

Zhou X.-C.,Chongqing Medical University | Sun X.-N.,Hainan Provincial General Hospital | Yang B.,Chongqing Medical University | Huang B.-L.,Hainan Provincial General Hospital | And 4 more authors.
World Chinese Journal of Digestology | Year: 2015

AIM: To detect the expression of heat shock protein 70 (HSP70) in intestinal tissue of mice with post-infectious irritable bowel syndrome (PI-IBS). METHODS: A mouse model for PI-IBS was established by infection of mice with trichinella spiralis in vivo. The inflammatory score of the intestine of PI-IBS mice was calculated, and abdominal withdrawal reflex (AWR) and colon transportation test (CTT) were used to evaluate the clinical features of the PI-IBS mice. Intestinal expression of HSP70 was measured by immunohistochemistry. Spearman correlation analysis was carried out to assess the relationship between the expression of HSP70 and the clinical features of PI-IBS. RESULTS: The PI-IBS mouse model was established successfully. On the 8th week, there was no visible inflammation in the intestine (ileal inflammation score: P > 0.05), whereas the clinical features significantly changed, including increased AWR score (P < 0.01) and decreased CTT score (P < 0.01). The expression of HSP70 significantly increased in the ileum but not in the colon of PI-IBS mice at the 8th week (ileum: P < 0.01; colon: P > 0.05). The expression of HSP70 in the ileum, but not in the colon, was remarkably related with the clinical features of PI-IBS mice, including the negative relationship with AWR (P < 0.01), and the positive relationship with CTT (P < 0.01). CONCLUSION: HSP70 might be associated with low-grade intestinal inflammation induced visceral hypersensitivity and abnormal intestinal motility in mice with PI-IBS. © 2015 Baishideng Publishing Group Inc. All rights reserved.

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