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Wu R.,Central South University | Zhou Q.,Central South University | Lin S.,Hainan Province Peoples Hospital | Ao X.,Central South University | And 2 more authors.
Journal of Central South University (Medical Sciences) | Year: 2010

Objective To observe the effect of Cordceps Sinensis (CS ) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the kidney of spontaneously hypertensive rats ( SHR ), and to investigate the mechanism of CS. Methods Male SHRs (23 week old ) were randomly divided into 4 groups ; a group without any treatment ( Group S ), a group treated with Cordceps sinensis at 4 g/ ( kg · d ) ( Group C ), a group treated with fosinopril at 10 mg/ ( kg · d ) ( Group F ), and a group received daily intragastric administration of CS at 4g/ ( kg · d ) and fosinopril at 10 mg/ ( kg · d ) ( Group CF ) . At the same time, 6 male WKY rats were used as normals controls. At the end of 8 weeks, all rats were sacrificed. Serum creatinine ( Scr), 24 h urinary protein count, and the expression of ICAM-1 and VCAM-1 were examined by immunohistochemical technique and RT-PCR. Results Compared with the WKY rats, blood pressure, 24 h urinary protein count, Scr, and the expression of ICAM-1 andVCAM-1 in the kidney of SHR significantly increased (P < 0.05 ) .Compared with Group S, blood pressure decreased after treatment by fosinopril (P<0.05). Compared with Group S, the levels of Scr, 24 h urinary protein count, and glomerular lesion were significantly reduced in the CS and/or fosinopril treatment group. The expression of ICAM-1 and VCAM-1 was significantly decreased in these groups (P <0.05 ) . Conclusion CS may play a role in the protection and anti-fibrosis in the process of renal injury in SHR through reducing the expression of ICAM -1 and VCAM -1. Source

To evaluate the efficacy and safety of the chemotherapy program of docetaxel combined with lobaplatin for Chinese patients with pulmonary and hepatic metastasis of nasopharyngeal carcinoma (NPC). This study included 37 NPC patients with pulmonary and hepatic metastasis. The chemotherapy program included docetaxel (75 mg/m, day 1) plus lobaplatin (30 mg/m, day 1). Cycle repetition was every 21 days. Patients were monitored for 7–41 months, with a median follow-up duration of 18 months. The total efficiency of this group was 67.6% and the disease control rate was 81.1%. The median progression-free survival was 9.4 months (95% confidence interval, 6.8–14.3 months), the median overall survival was 18.3 months (95% confidence interval, 13.7–22.8 months), and the 2-year survival rate was 37.8%. The main hematological toxicities were leukopenia (91.9%), anemia (81.1%), and thrombocytopenia (70.3%); other adverse reactions were mild. Changes in Epstein–Barr-DNA levels can basically reflect the dynamic changes in the efficacy of chemotherapy. Docetaxel combined with lobaplatin has a favorable outcome for the treatment of pulmonary and hepatic metastatic NPC. It has been a convenient regimen with tolerable toxicity. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved. Source

Xue H.,Qingdao University | Lin F.,Hainan Province Peoples Hospital | Tan H.,Linyi Peoples Hospital | Zhu Z.-Q.,Shanghai JiaoTong University | And 2 more authors.
PLoS ONE | Year: 2016

Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis and low five-year survival rate. A strong and effective CD4+ T cell-mediated cytotoxicity was associated with better survival and low recurrence rate in HCC, but the regulatory mechanism that controls CD4+ T cell cytotoxicity in HCC patients is not fully examined. Given that IL-10-expressing B cells could suppress the inflammation of cytotoxic CD8+ T cells, T helper 1 (Th1) cells and Th17 cells, while promoting regulatory T (Treg) cell differentiation, we examined the role of IL-10-expressing B cells in HBV-related HCC patients. We found that compared to healthy controls, HCC patients exhibited significantly higher frequencies of IL-10-expressing B cells, which were negatively correlated with the frequencies of granzyme A, granzyme B, and perforin expressing CD4+ T cells. Surface molecule Tim-1 was preferentially expressed on IL-10-expressing B cells. Therefore, we separated total B cells into Tim-1+ and Tim-1+ B cells. CD4+ T cells incubated with Tim-1+ B cells exhibited significantly reduced levels of granzyme A, granzyme B and perforin expression, compared to the CD4+ T cells incubated with Tim-1+ B cells. Antagonizing IL-10 in culture rescued CD4+ T cell cytotoxicity. Compared to that in peripheral blood, the level of IL-10-expressing B cells were further upregulated in resected tumor, while the level of CD4+ cytotoxic T cells was downregulated. The negative correlations between IL-10-expressing B cells and CD4+ cytotoxic T cells were also observed in tumor-infiltrating cells. Together, our data revealed an additional antitumor mechanism mediated by IL-10-expressing B cells. © 2016 Xue et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Fu Z.,Hainan Province Peoples Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To study the effects of subcutaneous allergen immunotherapy by Alutard SQ allergy vaccination in management of perennial allergic rhinitis. Thirty-eight cases of the perennial allergic rhinitis received the subcutaneous allergen immunotherapy by Alutard SQ allergy vaccination for 3 years or more than 3 years. The clinical symptoms and signs were compared before and after treatment. Good result were obtained in 21 of 38 cases, 7 cases effective, and 10 cases ineffective. Total effective rate was 73.68%. The subcutaneous allergen immunotherapy by Alutard SQ allergy vaccination was very effective for the perennial allergen rhinitis. Source

Wang B.,Central South University | Li Y.,Central South University | Tan F.,Central South University | Xiao Z.,Hainan Province Peoples Hospital
Tumor Biology | Year: 2016

Sex-determining region Y-related high-mobility group box 4 (SOX4) has been proven to serve as a critical role in cancer progression. However, the pathological role of SOX4 in colorectal cancer (CRC) remains unknown. The aim of this study was to investigate the role of SOX4 in CRC. In this study, we investigated the expression of SOX4 in CRC tissues by immunohistochemistry, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blot. We also evaluated the effect of SOX4 on cell proliferation and invasion by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and transwell assay. The SOX4 messenger RNA (mRNA) and protein expression were markedly higher in CRC tissues compared with adjacent normal mucosa tissues. Inhibition of SOX4 could suppress CRC cell proliferation, and invasion in vitro. Our findings indicate that targeting SOX4 might provide a new therapeutic modality for the treatment of CRC patients. © 2016 International Society of Oncology and BioMarkers (ISOBM) Source

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