Hainan Medical College

Haikou, China

Hainan Medical College

Haikou, China
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Wu S.,Hainan Medical College
Journal of Nanoscience and Nanotechnology | Year: 2017

Fabrication of a kind of glypican 3 targeted nanobubbles for contrast enhanced ultrasound imaging was reported in this work. The targeting nanobubbles were formatted using PLGA as shell and perfluorocarbon as core, with a double emulsion (water/oil/water) evaporation process, and conjugating peptide as targeting ligand. The Peptide/PLGA/NBs were characterized in vitro. Results: The mean diameter of Peptide/PLGA NBs was 773.6±185.4 nm and zeta potential was -22.3±3.3 mV, their sizes were nanoscale in general, their uniform was not very well, and diameters were discrete. Ultrasonic imaging evaluation of Peptide/PLGA NBs in vitro showed which had significant differences of echo intensities with different concentration gradients. Peptide/PLGA NBs could target GPC3 of HCC cells in vitro. Conclusions: The GPC3 targeted nanobubbles (Peptide/PLGA NBs) have been developed, which have potential to develop into ultrasound contrast agents. Optimization of the Peptide/PLGA NBs is necessary to decrease the size of NBs and to narrow dispersity of NBs, and further research needs to be done to evaluate the characteristics of Peptide/PLGA NBs in vivo. Copyright © 2017 American Scientific Publishers All rights reserved.


Chen F.,Hainan Medical College | Wen Q.,Hainan Medical College | Jiang J.,Hainan Medical College | Li H.-L.,Hainan Medical College | And 3 more authors.
Journal of Ethnopharmacology | Year: 2016

Ethnopharmacological relevance A wealth of information is emerging about the impact of gut microbiota on human health and diseases such as cardiovascular diseases, obesity and diabetes. As we learn more, we find out the gut microbiota has the potential as new territory for drug targeting. Some novel therapeutic approaches could be developed through reshaping the commensal microbial structure using combinations of different agents. The gut microbiota also affects drug metabolism, directly and indirectly, particularly towards the orally administered drugs. Herbal products have become the basis of traditional medicines such as traditional Chinese medicine and also been being considered valuable materials in modern drug discovery. Of note, low oral bioavailability but high bioactivity is a conundrum not yet solved for some herbs. Since most of herbal products are orally administered, the herbs' constituents are inevitably exposed to the intestinal microbiota and the interplays between herbal constituents and gut microbiota are expected. Emerging explorations of herb-microbiota interactions have an opportunity to revolutionize the way we view herbal therapeutics. The present review aims to provide information regarding the health promotion and/or disease prevention by the interplay between traditional herbs with low bioavailability and gut microbiota through gut microbiota via two different types of mechanisms: (1) influencing the composition of gut microbiota by herbs and (2) metabolic reactions of herbal constituents by gut microbiota. Materials and methods The major data bases (PubMed and Web of Science) were searched using "gut microbiota", "intestinal microbiota", "gut flora", "intestinal flora", "gut microflora", "intestinal microflora", "herb", "Chinese medicine", "traditional medicine", or "herbal medicine" as keywords to find out studies regarding herb-microbiota interactions. The Chinese Pharmacopoeia (2010 edition, Volume I) was also used to collect the data of commonly used medicinal herbs and their quality control approaches. Results Among the 474 monographs of herbs usually used in the Chinese Pharmacopoeia, the quality control approach of 284 monographs is recommended to use high-performance liquid chromatography approach. Notably, the major marker compounds (>60%) for quality control are polyphenols, polysaccharides and saponins, with significant oral bioavailability conundrum. Results from preclinical and clinical studies on herb-microbiota interactions showed that traditional herbs could exert heath promotion and disease prevention roles via influencing the gut microbiota structure. On the other hand, herb constituents such as ginsenoside C-K, hesperidin, baicalin, daidzin and glycyrrhizin could exert their therapeutic effects through gut microbiota-mediated bioconversion. Conclusions Herb-microbiota interaction studies provide novel mechanistic understanding of the traditional herbs that exhibit poor oral bioavailability. "Microbiota availability" could be taken consideration into describing biological measurements in the therapeutic assessment of herbal medicine. Our review should be of value in stimulating discussions among the scientific community on this relevant theme and prompting more efforts to complement herb-microbiota interactions studies. © 2015 Elsevier Ireland Ltd. All rights reserved.


Wu C.-F.,Sichuan University | Tan G.-H.,Hainan Medical College | Ma C.-C.,Sichuan University | Li L.,Sichuan University
Journal of Genetics and Genomics | Year: 2013

Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF) and aberrant expression of long non-coding RNAs (lncRNAs) are associated with mouse and human tumors. To identify the PSF-binding lncRNA involved in human oncogenesis, we screened a nuclear RNA repertoire of human melanoma cell line, YUSAC, through RNA-SELEX affinity chromatography. A previously unreported lncRNA, termed as Llme23, was found to bind immobilized PSF resin. The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines. Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells, accompanied by the repressed expression of proto-oncogene Rab23. These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding lncRNA with human melanoma. © 2013.


Liu X.,Sun Yat Sen University | Liu X.,Hainan Medical College | Xie C.,Sun Yat Sen University
Molecular and Cellular Biochemistry | Year: 2012

Cardiovascular disease is the leading cause of morbidity and mortality in patients with moderate-to-severe chronic obstructive pulmonary disease (COPD). More than 44% of these patients present with generalized atherosclerosis at autopsy. It is accepted that endothelial progenitor cells (EPCs) participate in the repair of dysfunctional endothelium and thus protects against atherosclerosis. However, whether COPD affects the repairing capacity of EPCs is unknown. Therefore, the objective of this study was to determine whether and how EPCs are involved in the vascular repair process in patients with COPD. In our study, EPCs from 25 COPD and 16 control patients were isolated by Ficoll density-gradient centrifugation and identified using fluorescence activated cell sorting. Transwell Migratory Assay was performed to determine the number of EPC colony-forming units and the adherent capacity late-EPCs to human umbilical vein endothelial cells. Following arterial damage in NOD/SCID mice, the number of EPCs incorporated at the injured vascular site was determined using a fluorescence microscope. We found that the number of EPC clusters and cell migration, as well as the expression of CXCR4, was significantly decreased in patients with COPD. Additionally, the number of late-EPCs adherent to HUVEC tubules was significantly reduced, and fewer VEGFR2 +-staining cells were incorporated into the injured site in COPD patients. Our study demonstrates that EPC capacity of repair was affected in COPD patients, which may contribute to altered vascular endothelium in this patient population. © 2011 Springer Science+Business Media, LLC.


Yang S.,Hubei University | Yang S.,Hainan Medical College | Chen X.,Hubei University
International Journal of Oral and Maxillofacial Surgery | Year: 2012

Epithelial-myoepithelial carcinoma (EMC) is a rare low-grade salivary gland malignancy of presumed intercalated duct origin comprising 1% of all salivary gland tumours. High grade transformation (HGT) in EMC is a recently recognised entity with only a few cases reported in the literature. The authors report an additional case of EMC with HGT involving the submandibular gland. The patient was a 60-year-old woman who requested examination of the rapid growth of a mass in the left submandibular area, which she had first noticed 20 years previously. Histologically, the tumour had two distinct carcinomatous components. One component had features of a low grade EMC. The second component consisted of polygonal cells, arranged in a solid and nested pattern, with marked nuclear pleomorphism, brisk mitotic activity, and frequent necrosis. The Ki-67 labelling index of the EMC component was 9%, and that of the high grade component was 40%. The patient developed multiple pulmonary metastases 15 months after surgery. The aggressive behaviour of EMC with HGT suggests that it is important to recognise this variant of EMC to avoid misdiagnosis and inappropriate treatment. © 2011 International Association of Oral and Maxillofacial Surgeons.


Lin J.L.,Hainan Medical College
Advanced Materials Research | Year: 2014

Based on existing researches at home and abroad, an intensive study of ECG signal preprocessing, feature extraction, feature analysis and feature weight analysis was made in the Paper neural network classifier was designed to realize the ECG identification and it was optimized by GA algorithm and DNA algorithm. The main research was concluded as follows. Firstly, extracting the preprocessing and feature of ECG signal. We have analyzed the frequency of ECG signal and the noise signal included by using wavelet and wavelet threshold methods filter the low and high frequency noise in ECG signal. Secondly, analyzing weight of ECG feature and selecting the optimal feature subset. Evaluated by the accuracy rate of BP neural network classification, the optimal characteristics for identification subset is determined then. Thirdly, designing and optimizing the neural network classifier. As the BP neural network has the Problems of easily falling into local minimum and being not convergence, GA and DNA algorithm are used to optimize it. © (2014) Trans Tech Publications, Switzerland.


Li Y.,Chinese Academy of Sciences | Fei X.,Chinese Academy of Sciences | Fei X.,Hainan Medical College | Deng X.,Chinese Academy of Sciences
Biomass and Bioenergy | Year: 2012

Microalgae usually accumulate triacylglycerols (TAGs) under stress conditions, especially during nitrogen (N) starvation. However, little information is available regarding the underlying mechanism. In the present study, a green algae Micractinium pusillum accumulated significant amounts of TAGs after 6 d of N starvation. Aiming at fundamental elucidation of the molecular mechanisms involved in N starvation-induced TAG accumulation, the global gene expression changes were identified by using suppression subtractive hybridization (SSH). In total, 290 expressed sequence tags (ESTs) showed significant differential expression between the treated and control samples. The expression profiles of 19 ESTs have been confirmed by quantitative real-time PCR (qPCR). Homology analysis showed that the 174 N deficiency-induced ESTs were mainly involved in carbohydrate metabolism, pyruvate and acetyl-CoA synthesis, isoprenoids biosynthesis, and TAG degradation, as well as a severe down-regulation in the expression of 116 genes related to photosynthesis, cell growth, amino acid synthesis and cell cycle regulation. A significant decrease in carbohydrate content was also observed in the N-deficient algal cells. These results suggested that the carbon sources for TAG biosynthesis were largely derived from carbohydrate metabolism rather than from photosynthesis. The results of the qPCR showed that, compared to the control, the transcript levels of genes related to pyruvate and acetyl-CoA synthesis were dramatically increased over 100 times after N starvation, which suggests that pyruvate and acetyl-CoA play important roles in driving carbon flow into TAG biosynthesis. © 2012 Elsevier Ltd.


Zhang H.,Hainan Medical College | Yang H.,Nanjing Medical University | He S.,Nanjing Medical University
Cellular Physiology and Biochemistry | Year: 2010

Tumor necrosis factor (TNF) is a proinflammatory cytokine which has been shown to be actively involved in the pathogenesis of allergic inflammation. However, little is known of the effects of TNF on cytokine secretion and protease activated receptor (PAR) expression in mast cells. In the present study, we examined potential influence of TNF on IL-4 and IL-12 release from P815 cells and PAR expression in P815 cells by using flow cytometry analysis, quantitative real time PCR, ELISA and cellular activation of signaling ELISA (CASE) techniques. The results showed that TNF induced up to 2.7-fold increase in IL-4, but not IL-12 release from P815 cells, and PAR-2 antagonist peptide FSLLRY-NH 2 and PAR-4 antagonist peptide trans-cinnamoyl (tc)-YPGKF-NH 2 did not affect TNF induced IL-4 release. Approximately up to 2.4 and 2.3 fold increases in expression of PAR-2 and PAR-4 were observed when cells were incubated with TNF. Pretreatment of cells with TNF for 60 min enhanced trypsin and tryptase induced PAR-2 expression by 2.4 and 2.3 fold; PAR-3 expression by 1.6 and 1.7 fold and PAR-4 expression by 1.6 and 1.7 fold, respectively. LY294002, an inhibitor PI3K abolished TNF induced IL-4 release and phosphorylation of Akt in P815 cells, indicating Akt cell signalling pathway is involved in the event. In conclusion, TNF can stimulate IL-4 release from mast cells through an Akt cell signalling pathway dependent, but PAR independent mechanism. TNF may serve as a regulator for IL-4 production and PAR expression, and through which participates in the mast cell related inflammation. © 2010 S. Karger AG, Basel.


Zhan Y.,Zhengzhou University | Li H.,Hainan Medical College | Chen Y.,Zhengzhou University
Journal of Hazardous Materials | Year: 2010

Copper hydroxyphosphate was synthesized hydrothermally and characterized by XRD and SEM. The peroxide degradation of azo dye on this material was evaluated by examining initial pH, catalyst loading, H2O2 dosage, initial dye concentration and temperature. Although copper hydroxyphosphate is a low surface area material without micropores or mesopores, it shows considerable activity for oxidative degradation of azo dyes under near-neutral pH conditions. A catalyst with such simple and clear structure may be a suitable model material for research on the mechanism of generating hydroxyl radicals and heir destruction of organic molecules. © 2010 Elsevier B.V.


Wiwanitkit V.,Hainan Medical College
Asian Pacific Journal of Cancer Prevention | Year: 2010

Colon cancer is an important gastrointestinal cancer that has increasing prevalence in the present day. Screening for colon cancer is accepted as a method for colon cancer prevention. There are many methods for screening for colon cancer. However, the best method is colonoscopy. Nevertheless, the simple classical method as occult blood test is still widely used in clinical practice. Here, the author appraises on colonoscopy with and without occult blood test pre-screening. The aim is to compare the cost effectiveness and cost utility between these two approaches. Based on the present appraisal, the colonoscopy without pre-screening occult blood test provides more cost effectiveness than colonoscopy with such pre-screening.

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