Wu C.-F.,Sichuan University |
Tan G.-H.,Hainan Medical College |
Ma C.-C.,Sichuan University |
Li L.,Sichuan University
Journal of Genetics and Genomics | Year: 2013
Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF) and aberrant expression of long non-coding RNAs (lncRNAs) are associated with mouse and human tumors. To identify the PSF-binding lncRNA involved in human oncogenesis, we screened a nuclear RNA repertoire of human melanoma cell line, YUSAC, through RNA-SELEX affinity chromatography. A previously unreported lncRNA, termed as Llme23, was found to bind immobilized PSF resin. The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines. Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells, accompanied by the repressed expression of proto-oncogene Rab23. These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding lncRNA with human melanoma. © 2013.
Liu X.,Sun Yat Sen University |
Liu X.,Hainan Medical College |
Xie C.,Sun Yat Sen University
Molecular and Cellular Biochemistry | Year: 2012
Cardiovascular disease is the leading cause of morbidity and mortality in patients with moderate-to-severe chronic obstructive pulmonary disease (COPD). More than 44% of these patients present with generalized atherosclerosis at autopsy. It is accepted that endothelial progenitor cells (EPCs) participate in the repair of dysfunctional endothelium and thus protects against atherosclerosis. However, whether COPD affects the repairing capacity of EPCs is unknown. Therefore, the objective of this study was to determine whether and how EPCs are involved in the vascular repair process in patients with COPD. In our study, EPCs from 25 COPD and 16 control patients were isolated by Ficoll density-gradient centrifugation and identified using fluorescence activated cell sorting. Transwell Migratory Assay was performed to determine the number of EPC colony-forming units and the adherent capacity late-EPCs to human umbilical vein endothelial cells. Following arterial damage in NOD/SCID mice, the number of EPCs incorporated at the injured vascular site was determined using a fluorescence microscope. We found that the number of EPC clusters and cell migration, as well as the expression of CXCR4, was significantly decreased in patients with COPD. Additionally, the number of late-EPCs adherent to HUVEC tubules was significantly reduced, and fewer VEGFR2 +-staining cells were incorporated into the injured site in COPD patients. Our study demonstrates that EPC capacity of repair was affected in COPD patients, which may contribute to altered vascular endothelium in this patient population. © 2011 Springer Science+Business Media, LLC.
Yang S.,Hubei University |
Yang S.,Hainan Medical College |
Chen X.,Hubei University
International Journal of Oral and Maxillofacial Surgery | Year: 2012
Epithelial-myoepithelial carcinoma (EMC) is a rare low-grade salivary gland malignancy of presumed intercalated duct origin comprising 1% of all salivary gland tumours. High grade transformation (HGT) in EMC is a recently recognised entity with only a few cases reported in the literature. The authors report an additional case of EMC with HGT involving the submandibular gland. The patient was a 60-year-old woman who requested examination of the rapid growth of a mass in the left submandibular area, which she had first noticed 20 years previously. Histologically, the tumour had two distinct carcinomatous components. One component had features of a low grade EMC. The second component consisted of polygonal cells, arranged in a solid and nested pattern, with marked nuclear pleomorphism, brisk mitotic activity, and frequent necrosis. The Ki-67 labelling index of the EMC component was 9%, and that of the high grade component was 40%. The patient developed multiple pulmonary metastases 15 months after surgery. The aggressive behaviour of EMC with HGT suggests that it is important to recognise this variant of EMC to avoid misdiagnosis and inappropriate treatment. © 2011 International Association of Oral and Maxillofacial Surgeons.
Lin J.L.,Hainan Medical College
Advanced Materials Research | Year: 2014
Based on existing researches at home and abroad, an intensive study of ECG signal preprocessing, feature extraction, feature analysis and feature weight analysis was made in the Paper neural network classifier was designed to realize the ECG identification and it was optimized by GA algorithm and DNA algorithm. The main research was concluded as follows. Firstly, extracting the preprocessing and feature of ECG signal. We have analyzed the frequency of ECG signal and the noise signal included by using wavelet and wavelet threshold methods filter the low and high frequency noise in ECG signal. Secondly, analyzing weight of ECG feature and selecting the optimal feature subset. Evaluated by the accuracy rate of BP neural network classification, the optimal characteristics for identification subset is determined then. Thirdly, designing and optimizing the neural network classifier. As the BP neural network has the Problems of easily falling into local minimum and being not convergence, GA and DNA algorithm are used to optimize it. © (2014) Trans Tech Publications, Switzerland.
Zhou H.,Cornell College |
Zheng S.,Hainan Medical College |
Truong L.D.,Cornell College |
Ro J.Y.,Cornell College |
And 2 more authors.
Human Pathology | Year: 2014
Summary Clear cell papillary renal cell carcinoma (CCP-RCC) has recently been recognized as a distinct subtype of renal cell carcinoma (RCC) due to its unique morphologic, immunohistochemical, and genetic features and indolent clinical behavior. However, the incidence of this tumor in a nephrectomy series for renal mass has not been fully investigated. Twelve cases of CCP-RCC were identified from a total of 290 consecutive partial (n = 137) or radical nephrectomies (n = 153) for RCC from 2010 to 2012 in our hospital. In this series, CCP-RCC was the fourth most common (4.1%) kidney tumor following clear cell (conventional) (70%), papillary (16.6%), and chromophobe (5.9%) RCCs. The average age of the CCP-RCC patients was 58.2 years (range, 18-81 years), with an equal sex distribution. Four cases (33.3%) were associated with end-stage renal disease. Of the 12 CCP-RCCs, 9 presented as solitary tumors; 2 coexisted with clear cell RCC; and 1 with papillary RCC. The average size of tumors was 2.5 cm (range, 0.8-6.0 cm). All tumors were pT1 (10 pT1a and 2 pT1b). Two cases were initially misclassified as clear cell RCC. Strong positive cytokeratin 7 stain and negative stains with α-methylacyl-CoA racemase and RCC marker differentiate CCP-RCC from low-grade clear cell RCC with similar histologic features. We conclude that CCP-RCC is a common renal neoplastic entity, representing the fourth most common (4.1%) RCC. It can be easily misclassified due to its overlapping features with low-grade clear cell RCC. In equivocal cases, immunohistochemical stains with a small panel of markers (cytokeratin 7, α-methylacyl-CoA racemase, RCC marker, or CD10) are warranted in making the correct histologic classification. © 2014 Elsevier Inc.
Li Y.,Chinese Academy of Sciences |
Fei X.,Chinese Academy of Sciences |
Fei X.,Hainan Medical College |
Deng X.,Chinese Academy of Sciences
Biomass and Bioenergy | Year: 2012
Microalgae usually accumulate triacylglycerols (TAGs) under stress conditions, especially during nitrogen (N) starvation. However, little information is available regarding the underlying mechanism. In the present study, a green algae Micractinium pusillum accumulated significant amounts of TAGs after 6 d of N starvation. Aiming at fundamental elucidation of the molecular mechanisms involved in N starvation-induced TAG accumulation, the global gene expression changes were identified by using suppression subtractive hybridization (SSH). In total, 290 expressed sequence tags (ESTs) showed significant differential expression between the treated and control samples. The expression profiles of 19 ESTs have been confirmed by quantitative real-time PCR (qPCR). Homology analysis showed that the 174 N deficiency-induced ESTs were mainly involved in carbohydrate metabolism, pyruvate and acetyl-CoA synthesis, isoprenoids biosynthesis, and TAG degradation, as well as a severe down-regulation in the expression of 116 genes related to photosynthesis, cell growth, amino acid synthesis and cell cycle regulation. A significant decrease in carbohydrate content was also observed in the N-deficient algal cells. These results suggested that the carbon sources for TAG biosynthesis were largely derived from carbohydrate metabolism rather than from photosynthesis. The results of the qPCR showed that, compared to the control, the transcript levels of genes related to pyruvate and acetyl-CoA synthesis were dramatically increased over 100 times after N starvation, which suggests that pyruvate and acetyl-CoA play important roles in driving carbon flow into TAG biosynthesis. © 2012 Elsevier Ltd.
Zhang H.,Hainan Medical College |
Yang H.,Nanjing Medical University |
He S.,Nanjing Medical University
Cellular Physiology and Biochemistry | Year: 2010
Tumor necrosis factor (TNF) is a proinflammatory cytokine which has been shown to be actively involved in the pathogenesis of allergic inflammation. However, little is known of the effects of TNF on cytokine secretion and protease activated receptor (PAR) expression in mast cells. In the present study, we examined potential influence of TNF on IL-4 and IL-12 release from P815 cells and PAR expression in P815 cells by using flow cytometry analysis, quantitative real time PCR, ELISA and cellular activation of signaling ELISA (CASE) techniques. The results showed that TNF induced up to 2.7-fold increase in IL-4, but not IL-12 release from P815 cells, and PAR-2 antagonist peptide FSLLRY-NH 2 and PAR-4 antagonist peptide trans-cinnamoyl (tc)-YPGKF-NH 2 did not affect TNF induced IL-4 release. Approximately up to 2.4 and 2.3 fold increases in expression of PAR-2 and PAR-4 were observed when cells were incubated with TNF. Pretreatment of cells with TNF for 60 min enhanced trypsin and tryptase induced PAR-2 expression by 2.4 and 2.3 fold; PAR-3 expression by 1.6 and 1.7 fold and PAR-4 expression by 1.6 and 1.7 fold, respectively. LY294002, an inhibitor PI3K abolished TNF induced IL-4 release and phosphorylation of Akt in P815 cells, indicating Akt cell signalling pathway is involved in the event. In conclusion, TNF can stimulate IL-4 release from mast cells through an Akt cell signalling pathway dependent, but PAR independent mechanism. TNF may serve as a regulator for IL-4 production and PAR expression, and through which participates in the mast cell related inflammation. © 2010 S. Karger AG, Basel.
Zhan Y.,Zhengzhou University |
Li H.,Hainan Medical College |
Chen Y.,Zhengzhou University
Journal of Hazardous Materials | Year: 2010
Copper hydroxyphosphate was synthesized hydrothermally and characterized by XRD and SEM. The peroxide degradation of azo dye on this material was evaluated by examining initial pH, catalyst loading, H2O2 dosage, initial dye concentration and temperature. Although copper hydroxyphosphate is a low surface area material without micropores or mesopores, it shows considerable activity for oxidative degradation of azo dyes under near-neutral pH conditions. A catalyst with such simple and clear structure may be a suitable model material for research on the mechanism of generating hydroxyl radicals and heir destruction of organic molecules. © 2010 Elsevier B.V.
Lv G.,Hainan Medical College
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases | Year: 2010
The full-length pBluescript II SK cDNA library of adult Spirometra erinaceieuropaei was constructed by using the SMART method. Data showed that 95.5% of the library was recombinant and the titer of the library was 1.06 x 10(6). The average insert size of the library was about 1.4 kb. Forty-eight randomly selected clones were sequenced. A set of 36 effective expressed sequence tags (ESTs) with the average size of 674 bp was obtained after excluding clones shorter than 450 bp. The unigenes occupied 58.3% of the 36 ESTs. The rate of full-length cDNAs were 57.7% (15/26). The high-quality of full-length cDNA library could be used for large scale EST sequencing.
Wiwanitkit V.,Hainan Medical College
Asian Pacific Journal of Cancer Prevention | Year: 2010
Colon cancer is an important gastrointestinal cancer that has increasing prevalence in the present day. Screening for colon cancer is accepted as a method for colon cancer prevention. There are many methods for screening for colon cancer. However, the best method is colonoscopy. Nevertheless, the simple classical method as occult blood test is still widely used in clinical practice. Here, the author appraises on colonoscopy with and without occult blood test pre-screening. The aim is to compare the cost effectiveness and cost utility between these two approaches. Based on the present appraisal, the colonoscopy without pre-screening occult blood test provides more cost effectiveness than colonoscopy with such pre-screening.