Hainan Branch of General Hospital of PLA

Hainan, China

Hainan Branch of General Hospital of PLA

Hainan, China
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Huang H.-B.,Hainan Branch of General Hospital of PLA | Zhang Y.-X.,Hainan Branch of General Hospital of PLA | Zhang S.-H.,The Second Affiliated Hospital Of Haerbin Medical University | Ma Z.-Z.,Peking University
Neurological Research | Year: 2015

Objectives: To calibrate and standardise an animal model of graded optic nerve injury (ONI) in rats to facilitate future inter-laboratory data comparisons, focussing on quantification of injury intensity, injury severity, and the correlation between them. Methods: A pair of cross-action forceps or a pair of artery clips was used to induce optic nerve (ON) crush injuries. A lever principle and a simplified method were used to measure the crushing force. The simplified method directly measured weights as an external force exerted on the tip of the forceps or clips, which was just sufficient to maintain a gap and was equivalent to the closing (crush) force. The impulse and averaged impulse were explored as physical quantities to compare injury intensities. Graded ONIs were made by crushing the ON for 3, 6, 12, 30 or 60 seconds by the cross-action forceps, or 5, 10 or 15 seconds by the artery clips. The injury severity was evaluated by counting surviving retinal ganglion cell (RGC) through applied FluoroGold to the superior colliculus and lateral geniculate body before ON crush, intact RGC counting by applied FluoroGold after ON crush, and ON axon counting. Results: Similar results were obtained by the lever principle method and the simplified method. The crushing force of the cross-action forceps and the artery clips was 148.0 gram force (gf) and 32.4 gf, respectively. The graded ONI animal models were successfully created in rats without retinal ischaemia post-trauma. The averaged impulse produced by the artery clips for 15 seconds was equal to that produced by a 3-second crush of the cross-action forceps. The correlation between injury intensity and injury severity was fitted for a power function. Discussion: Our results provide a simplified and effective means to quantify and analyse data from ON crush studies compared with previously reported animal models. © W. S. Maney and Son Ltd 2015.


Liu X.-M.,Navy General Hospital | Fu Q.,Hainan Branch of General Hospital of PLA | Mi W.-D.,General Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective: To explore the value of stroke volume variation (SVV), pulse pressure variation (PPV) and pleth variability index (PVI) in evaluating the volume status under pneumoperitoneum and their correlation. Methods: Twenty-six patients with stomach cancer receiving laparoscopy-assisted radical gastrectomy under general anesthesia were involved in this study. The indexes of SVV, PPV and PVI were monitored continuously after general anesthesia. A volume loading test was performed after pneumoperitoneum, and the hemodynamic parameters were determined before and after fluid infusion (6% HES, 7ml/kg), and the patients were divided into response group (n=16) and non-response group (n=10) according to whether the increase in stroke volume index (ΔSVI) was ≥15% or not. The receiver operator characteristic (ROC) curves of SVV, PPV and PVI were drawn to evaluate the value of SVV, PPV and PVI in evaluating volume status under artificial pneumoper itoneum and the correlation between them. Results: The analysis of ROC curves showed that SVV, PPV and PVI could all be used to estimate the volume status effectively, the area under the curve (AUC) of them was 0.978, 0.963 and 0.928 respectively, and there was a significant correlation between the three parameters (SVV and PPV: r=0.921, P<0.01; SVV and PVI: r=0.686, P<0.01; PPV and PVI: r=0.577, P<0.01). Conclusion: SVV, PPV and PVI could be used to accurately evaluate the volume status under artificial pneumoperitoneum, and there is a good correlation between them.


Zhang Y.-X.,Hainan Branch of General Hospital of PLA | Huang H.-B.,General Hospital of PLA | Wei S.-H.,General Hospital of PLA
Experimental and Therapeutic Medicine | Year: 2014

Pathological optic disc cupping (ODC) is predominantly referred to as glaucoma; however, it is not only glaucoma that leads to pathological optic disc excavation. A number of other nonglaucomatous diseases also result in optic atrophy and excavation of the optic disc. Therefore, in the present study, the etiology of nonglaucomatous optic disc cupping (NGODC) was analyzed and differentiated from glaucomatous optic disc cupping (GODC). The morphology and clinical data of 19 eyes, from 12 patients exhibiting NGODC, were analyzed. Of the 12 cases, none were diagnosed with glaucoma, four presented with optic neuritis, one with Devic's disease, one with Leber's hereditary optic neuropathy, two with pituitary adenoma, one with basal ganglia cerebral hemorrhage, one with cilioretinal artery occlusion associated with central retinal vein occlusion, one with central retinal artery occlusion and the remaining patient exhibited optic nerve injuries. The key features that differentiated NGODC from GODC were the color of the optic disc rim and the correlation between visual field defects and the disc appearance. The focally notched disc also aided in distinguishing between the two disorders. The results of the present study indicated that it is critical to acknowledge that nonglaucomatous diseases also lead to ODC and that distinguishing between them is necessary.


Zhang F.,Hainan Branch of General Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To analyze the renal relative blood flow value (rBFV) and image quality in normal adults using single-shot fast spin echo, flow sensitive invention recovery (SSFSE-FAIR) magnetic resonance (MR) sequence and echo planar imaging, and flow sensitive invention recovery (EPI-FAIR) MR sequence, and assess its value for clinical application in routine renal examination. Forty volunteers (25 male and 15 female adults, aged 30 to 62 years) with normal renal function were included in this prospective study. All the subjects underwent 3.0 Tesla MR scanning using 3 MR scan modes, namely breath-holding EPI-FAIR, breath-holding SSFSE-FAIR and free breathing SSFSE-FAIR. SSFSE-FAIR without breath-holding was capable of differentiating the renal cortex and medulla with the corresponding rBFVs of 111.48∓9.23 and 94.98∓3.38, respectively. Breath-holding SSFSE-FAIR and EPI-FAIR failed to distinguish the borders of the renal cortex and medulla. The EPI-FAIR rBFV of mixed cortex and medulla value was 178.50∓17.17 (95%CI: 167.59, 189.41). Breath-holding SSFSE-FAIR and EPI-FAIR can not distinguish the renal cortex and medulla due to a poor spatial resolution but can be used for rough evaluation of renal blood perfusion. Free breathing SSFSE-FAIR with an improved spatial resolution allows evaluation of the status of renal perfusion of the cortex and medulla.


Huang H.-B.,Chinese PLA General Hospital | Zhang Y.-X.,Hainan Branch of General Hospital of PLA
Experimental and Therapeutic Medicine | Year: 2014

Pigmented paravenous retinochoroidal atrophy (PPRCA) is an uncommon disease characterized by perivenous aggregations of pigment clumps associated with peripapillary and radial zones of retinochoroidal atrophy that are distributed along the retinal veins. Patients are usually asymptomatic and the disease process is non-progressive or slow and subtly progressive. It is commonly bilateral and symmetric. The cause of the condition may be unknown or idiopathic, although a dysgenetic, degenerative, hereditary etiology or even an inflammatory cause has been hypothesized. A non-inflammatory cause is referred to as primary, while inflammation-associated PPRCA is referred to as secondary or pseudo PPRCA. The present study reviewed and summarized the features of PPRCA.


Li G.,Hainan Branch of General Hospital of PLA | Wang Q.,Hainan Branch of General Hospital of PLA | Lin T.,Hainan Branch of General Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2014

OBJECTIVE: To assess the relationship between protease-activated receptor 1 (PAR1) expression in the basilar artery and cerebral vasospasm (CVS) in a rat model of subarachnoid hemorrhage (SAH).METHODS: Twenty-four SD rats were randomized into normal control group, SAH 3-days group, SAH 5-days group and SAH 7-days group. Rat models of SAH were established by two injections of blood into the cisterna magna and the behavioral changes of the rats were observed. The basilar arteries were taken at 3, 5, or 7 days following the modeling for measuring the cross-sectional area of the basilar artery and for immunohistochemical detection of PAR1 expression.RESULTS: The SAH model rats, especially those in SAH 3-days group, presented with obvious neurological deficits, which was not found in the normal control group. CVS was not observed in the normal control group but occurred in the SAH model rats, which showed reduced cross-sectional area of the basilar artery and worsening spasm over time. The expression level of PAR1 tended to increase gradually in SAH 3-days, SAH 5-days and SAH 7-days groups. Pearson correlation analysis showed an inverse correlation between the expression of PAR1 and the cross-sectional area of the basilar artery (r=-0.779, P<0.01).CONCLUSIONS: The expression of PAR1 increases significantly in rat basilar artery wall following SAH in positive correlation with the severity of CVS, suggesting the role of thrombin in the pathological process of CVS after SAH.


PubMed | Chinese PLA General Hospital and Hainan Branch of General Hospital of PLA
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2014

Pigmented paravenous retinochoroidal atrophy (PPRCA) is an uncommon disease characterized by perivenous aggregations of pigment clumps associated with peripapillary and radial zones of retinochoroidal atrophy that are distributed along the retinal veins. Patients are usually asymptomatic and the disease process is non-progressive or slow and subtly progressive. It is commonly bilateral and symmetric. The cause of the condition may be unknown or idiopathic, although a dysgenetic, degenerative, hereditary etiology or even an inflammatory cause has been hypothesized. A non-inflammatory cause is referred to as primary, while inflammation-associated PPRCA is referred to as secondary or pseudo PPRCA. The present study reviewed and summarized the features of PPRCA.


PubMed | Hainan Branch of General Hospital of PLA
Type: Journal Article | Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2015

To investigate effect of CD4(+) CD25(+) Foxp3(+) Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms.Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4(+) CD25(+) Foxp3(+) Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs was analyzed using flow cytometry; peripheral blood IL-10 and TGF- levels were tested using double antibody sandwich ELISA.Compare with the model group, the mice in the treatment group showed significantly reduced muscular inflammatory cell infiltration, increased blood levels of IL-10 and TGF- (P<0.05), and increased expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs (P<0.05).CD4(+) CD25(+) Foxp3(+) Tregs reinfusion produces therapeutic effect in mice with EAM by increasing peripheral blood IL-10 and TGF- levels and PD-1 and CTLA-4 expressions in spleen CD4(+) CD25(+) Foxp3(+) Tregs.


PubMed | Hainan Branch of General Hospital of PLA
Type: Journal Article | Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2014

To assess the relationship between protease-activated receptor 1 (PAR1) expression in the basilar artery and cerebral vasospasm (CVS) in a rat model of subarachnoid hemorrhage (SAH).Twenty-four SD rats were randomized into normal control group, SAH 3-days group, SAH 5-days group and SAH 7-days group. Rat models of SAH were established by two injections of blood into the cisterna magna and the behavioral changes of the rats were observed. The basilar arteries were taken at 3, 5, or 7 days following the modeling for measuring the cross-sectional area of the basilar artery and for immunohistochemical detection of PAR1 expression.The SAH model rats, especially those in SAH 3-days group, presented with obvious neurological deficits, which was not found in the normal control group. CVS was not observed in the normal control group but occurred in the SAH model rats, which showed reduced cross-sectional area of the basilar artery and worsening spasm over time. The expression level of PAR1 tended to increase gradually in SAH 3-days, SAH 5-days and SAH 7-days groups. Pearson correlation analysis showed an inverse correlation between the expression of PAR1 and the cross-sectional area of the basilar artery (r=-0.779, P<0.01).The expression of PAR1 increases significantly in rat basilar artery wall following SAH in positive correlation with the severity of CVS, suggesting the role of thrombin in the pathological process of CVS after SAH.


PubMed | Chinese PLA General Hospital and Hainan Branch of General Hospital of PLA
Type: Journal Article | Journal: British journal of haematology | Year: 2016

The features of graft-versus-host disease (GVHD) were compared between patients who underwent myeloablative conditioning and received a peripheral blood stem cell transplant (PBSCT) from either a haploidentical donor (HID) or a matched sibling donor (MSD) during the same period of time. The HID group included more patients with advanced disease. Both groups received the same GVHD prophylaxis with the addition of antithymoglobulin (ATG) in HID group. Higher cumulative incidences (CI) of acute GVHD grade 2-4 (351% vs. 139%, P=0003), similar CI of grade 3-4 (145% vs. 98%, P=0595), less 3-year CI of extensive chronic GVHD (171% vs. 415%, P=0017) and less severe chronic GVHD (58% vs. 212%, P=0049) occurred in the HID group compared with the MSD group. There was no difference in the sites of the involved organs between these two groups. Higher 3-year CI of non-relapse mortality (240% vs. 102%, P=0014), relapse (390% vs. 226%, P=0032) and inferior disease-free survival (457% vs. 789%, P=0000) were recorded in the HID cohort compared with the MSD group. More HID patients had Karnofsky scores above 90 than those in MSD group (P=0016). In conclusion, ATG plays a key role in the unmanipulated HID PBSCT protocol, producing better quality of life in survivors.

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