Time filter

Source Type

Wang Q.,Hainan University | Wu Z.,Hainan Academy of Ocean and Fisheries science | Sun D.,Hainan University | Luo J.,Hainan University
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of the Ephippus orbis has been sequenced. The mitochondrial genome is 16 670 bp in length, containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and one control region. The gene order and the composition of E. orbis mitochondrial genome were similar to that of most other vertebrates. The overall nucleotides base composition of the heavy strand is A (27.17%), G (16.41%), C (31.46%), and T (24.96%). With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. Seen from the phylogenetic tree (Figure 1), E. orbis, Platax teira, and Platax orbicularis from the same family (Ephippidae) clustered into one branch and were significantly divergent from the other families of closely related fish species. © 2015 Taylor & Francis.


Lu Q.,Ocean University of China | Liu T.,Ocean University of China | Tang X.,Hainan Academy of Ocean and Fisheries science | Dong B.,Ocean University of China | And 2 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2016

Coral has strong regeneration ability, which has been applied for coral production and biodiversity protection via tissue ball (TB) culture. However, the architecture, morphological processes, and effects of environmental factors on TB formation have not been well investigated. In this study, we first observed TB formation from the cutting tentacle of scleractinia coral Goniopora lobata and uncovered its inner organization and architecture by confocal microscopy. We then found that the cutting tentacle TB could self-organize and reform a solid TB (sTB) in the culture media. Using chemical drug treatment and dissection manipulation approaches, we demonstrated that the mechanical forces for bending and rounding of the cutting fragments came from the epithelial cells, and the cilia of epithelial cell played indispensable roles for the rounding process. Environmental stress experiments showed that high temperature, not CO2-induced acidification, affected TB and sTB formation. However, the combination of high temperature and acidification caused additional severe effects on sTB reformation. Our studies indicate that coral TB has strong regeneration ability and therefore could serve as a new model to further explore the molecular mechanism of TB formation and the effects of environmental stresses on coral survival and regeneration. © 2016 The Society for In Vitro Biology


Li W.-D.,Hainan Academy of Ocean and Fisheries science | Huang M.,Hainan Academy of Ocean and Fisheries science | Lu W.-G.,Xiamen University | Chen X.,Guangxi Mangrove Research Center | And 4 more authors.
PLoS ONE | Year: 2015

The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone. © 2015 Li et al.


PubMed | Hainan Academy of Ocean and Fisheries science, Guangxi Mangrove Research Center and Xiamen University
Type: Journal Article | Journal: PloS one | Year: 2015

The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone.


Guo Z.K.,Chinese Academy of Sciences | Wang R.,Hainan Academy of Ocean and Fisheries science | Huang W.,Nanjing University | Li X.N.,CAS Kunming Institute of Botany | And 3 more authors.
Beilstein Journal of Organic Chemistry | Year: 2015

An unusual C18 norditerpenoid, aspergiloid I (1), was isolated from the culture broth of Aspergillus sp. YXf3, an endophytic fungus derived from Ginkgo biloba. Its structure was unambiguously established by analysis of HRMS-ESI and spectroscopic data, and the absolute configuration was determined by low-temperature (100 K) single crystal X-ray diffraction with Cu Kα radiation. This compound is structurally characterized by a new carbon skeleton with an unprecedented 6/5/6 tricyclic ring system bearing an α,β-unsaturated spirolactone moiety in ring B, and represents a new subclass of norditerpenoid, the skeleton of which is named aspergilane. The hypothetical biosynthetic pathway for 1 was also proposed. The cytotoxic, antimicrobial, anti-oxidant and enzyme inhibitory activities of 1 were evaluated. © 2014 Guo et al; licensee Beilstein-Institut. License and terms: see end of document.


Dong J.-D.,CAS South China Sea Institute of Oceanology | Dong J.-D.,Hainan Academy of Ocean and Fisheries science | Li Y.-C.,Hainan Academy of Ocean and Fisheries science | Wang Y.-S.,CAS South China Sea Institute of Oceanology | And 4 more authors.
Aquatic Ecosystem Health and Management | Year: 2015

The Xisha Islands, located off Sanya, South China Sea, are a group of islands administered by Hainan Province of the People's Republic of China. Due to little human activity, they are well preserved. Such conditions suggest that changes in ecosystem are mostly related to natural behavior. A baseline of water quality was established in May and July, 2012. The results of multivariate analysis showed changes in nutrients as the most significant variable in describing water quality. Hydrologic heterogeneity showed little change, but temporal characteristics presented distinguishable variations. This information may be valuable for socioeconomic development and human health in Xisha island waters. © 2015 AEHMS.


Li Y.-C.,Hainan Academy of Ocean and Fisheries science | Yang Y.,Hainan Academy of Ocean and Fisheries science | Zheng X.-Q.,State Oceanic Administration | Chen S.-Q.,Hainan Academy of Ocean and Fisheries science
Chinese Journal of Ecology | Year: 2015

Several parameters including species biodiversity, coverage, diseases, and recruitment of corals were investigated in Houhai waters, Sanya, Hainan, by the manta tow and line intercept transect methods to assess the health status of coral reef ecosystems, and the main factors affecting the health status of coral reef ecosystems were analyzed. The results showed that there were 54 species of scleractinia corals in Houhai waters and the mean coverage of corals reached more than 50%. Few coral diseases and low mortality were observed. The recruitment of corals was very high, up to 4.5 ind·m-2. The condition index of coral reef ecosystems ranged from 1.75 to 2.27, indicating that the coral reef ecosystems in Houhai waters were in a healthy status. Analysis showed that seagrass bed and seaweed in the shallow area, abundant grazers and the upwelling in summer should be mainly responsible for the healthy status of coral reef ecosystems. Massive seagrass and seaweed distributed in shallow water can absorb the land-sourced nutrients and stop the eutrophic water directly into coral reefs in Houhai waters. Abundant grazers such as Diadema setosum mediate the competitive interaction between reef-building corals and seaweeds, preventing the seaweed from threatening the growth of corals and preempting the space for coral settlements. The existence of the upwelling in summer maintains the suitable water temperature in Houhai water in summer, avoiding the coral bleaching. Accordingly, the interactions of multiple factors ensure the health of the coral reef ecosystem in Houhai waters. © 2015, editorial Board of Chinese Journal of Ecology. All rights reserved.


Wu Z.,Hainan Academy of Ocean and Fisheries science | Li X.,Hainan Academy of Ocean and Fisheries science
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of the Nemipterus virgatus has been sequenced. The mitochondrial genome is 16 992 bp in length, containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and one control region. The gene order and composition of N. virgatus mitochondrial genome was similar to that of most other vertebrates. The overall nucleotides base composition of the light strand is A (27.89%), G (26.61%), C (16.45%), T (29.05%). With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The tRNA-Ser2 gene lacked DHC arm and could not fold into a typical clover-leaf secondary structure. Seen from the phylogenetic tree, N. virgatus, Nemipterus japonicus, and Nemipterus bathybius from the same genus clustered into one branch. © 2015 Taylor & Francis.


Wang R.,Hainan Academy of Ocean and Fisheries science | Guo Z.K.,Chinese Academy of Sciences | Li X.M.,Hainan Academy of Ocean and Fisheries science | Chen F.X.,Hainan Academy of Ocean and Fisheries science | And 2 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

Two novel antibiotic spiculisporic acid analogues, named as spiculisporic acid F (1) and G (2), and two known compounds, (−)-spiculisporic acid (3) and secospiculisporic acid B (4), were isolated by bioactivity-guided fractionation from the fermentation broth of the sea urchin-derived Aspergillus candidus strain HDf2. Their structures were unambiguously established by comprehensive analysis of 1D and 2D NMR, and high-resolution MS spectra, and by comparison with known compounds. Biological experiments demonstrated that compounds 1 and 2 displayed antibacterial activity against Gram-negative Pseudomonas solanacearum and Gram-positive Staphylococcus aureus, but showed no cytotoxicity against SGC-7901 human gastric adenocarcinoma and SPC-A-1 human lung adenocarcinoma tumor cell lines. This is the first critical evidence identifying spiculisporic acid derivatives as a potential bio-control agent for the soil borne pathogen P. solanacearum (E. F. Smith) Smith. These findings provide further insight into the chemical and biological activity diversity of this class of compounds. © 2015, Springer International Publishing Switzerland.


PubMed | Hainan Academy of Ocean and Fisheries science
Type: Journal Article | Journal: Antonie van Leeuwenhoek | Year: 2015

Two novel antibiotic spiculisporic acid analogues, named as spiculisporic acid F (1) and G (2), and two known compounds, (-)-spiculisporic acid (3) and secospiculisporic acid B (4), were isolated by bioactivity-guided fractionation from the fermentation broth of the sea urchin-derived Aspergillus candidus strain HDf2. Their structures were unambiguously established by comprehensive analysis of 1D and 2D NMR, and high-resolution MS spectra, and by comparison with known compounds. Biological experiments demonstrated that compounds 1 and 2 displayed antibacterial activity against Gram-negative Pseudomonas solanacearum and Gram-positive Staphylococcus aureus, but showed no cytotoxicity against SGC-7901 human gastric adenocarcinoma and SPC-A-1 human lung adenocarcinoma tumor cell lines. This is the first critical evidence identifying spiculisporic acid derivatives as a potential bio-control agent for the soil borne pathogen P. solanacearum (E. F. Smith) Smith. These findings provide further insight into the chemical and biological activity diversity of this class of compounds.

Loading Hainan Academy of Ocean and Fisheries science collaborators
Loading Hainan Academy of Ocean and Fisheries science collaborators