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Guo X.,Central South University | Mao F.,Central South University | Wang W.,Central South University | Yang Y.,Central South University | Bai Z.,Haikou Municipal Peoples Hospital
ACS Applied Materials and Interfaces | Year: 2015

The objectives of this study are to prepare sulfhydryl-modified Fe3O4@SiO2 core/shell magnetic nanocomposites, assess their toxicity in vitro, and explore their potential application in the biomedical fields. Fe3O4 nanoparticles synthesized by facile solvothermal method were coated with SiO2 via the Stöber method and further modified by the meso-2,3-dimercaptosuccinic acid (DMSA) to prepare Fe3O4@SiO2@DMSA nanoparticles. The morphology, structure, functional groups, surface charge, and magnetic susceptibility of the nanoparticles were characterized by transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, zeta potential analysis, dynamic laser scattering, and vibrating sample magnetometer. Cytotoxicity tests and hemolysis assay were also carried out. Experimental results show that the toxicity of sulfhydryl-modified Fe3O4@SiO2 core/shell nanoparticles in mouse fibroblast (L-929) cell lines is between grade 0 and grade 1, and the material lacks hemolytic activity, indicating good biocompatibility of this Fe3O4@SiO2@DMSA nanocomposite, which is suitable for further application in biochemical fields. © 2015 American Chemical Society. Source

Li Q.,Hainan Nongken Hospital | Wang A.-Y.,Haikou Municipal Peoples Hospital | Xu Q.-G.,Hainan Nongken Hospital | Liu D.-Y.,Hainan Nongken Hospital | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: We performed co-culture and RNA interference to determine the role played by epidermal growth factor-like domain 7 (EGFL7) in the angiogenesis induced by neural stem cells and proposed the mechanism tentatively. Method: NSCs-HUVECs co-culture system was established using Transwell technique, and the interaction between the neural stem cells and the endothelial cells was simulated in vitro. The siRNA lentiviral vector targeting EGFL7 gene was constructed and transfected into the NSCs-HUVECs system. Thus the effect of EGFL7 gene on angiogenesis of the vascular endothelial cells (VECs) was discussed by proliferation, adhesion and tube formation tests of VECs. Results: After the silencing of EGFL7 gene, the EGFL7 expression level in HUVECs decreased and the adhesion capacity was inhibited significantly. Tube formation test indicated that no tubes were formed by the VECs after silencing of EGFL7 gene. Conclusion: EGFL7 played a key regulatory role in angiogenesis induced by neural stem cells by regulating the adhesion capacity of VECs. © 2016, E-Century Publishing Corporation. All rights reserved. Source

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