Haikou Key Laboratory of Environment Toxicology

Haikou, China

Haikou Key Laboratory of Environment Toxicology

Haikou, China
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Wang H.,Hainan University | Wang H.,Haikou Key Laboratory of Environment Toxicology | Cui L.,CAS Wuhan Botanical Garden | Cheng H.,Hainan University | And 6 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2017

Research on the kinetics of Benzo[a]pyrene (B[a]P) bioaccumulation in the clam Pinctada martensii and mussel Perna viridis showed that the initial rate of uptake was directly related to the PAH concentrations in the ambient environment. The uptake and depuration rate constants were different at the four B[a]P exposure levels, which indicated that the toxicokinetic rate constants mainly depended on the exposure levels of pollutants to the environment. In addition, the uptake rate constants of B[a]P were higher than the depuration rate constants in the entire experiment. The comparison demonstrated that mussels release B[a]P more rapidly than clams. The bioconcentration factors (BCFs) of B[a]P varied from 3335 to 12892 in the clam and 2373–6235 in the mussel. These findings on the bioaccumulation kinetics for petroleum hydrocarbons, in association with the critical body residue, will be valuable when choosing sensitive organisms to assess the potential ecotoxicological risk to the marine environment. © 2017 Springer Science+Business Media New York


Song Q.,Hainan University | Zheng P.,Hainan University | Qiu L.,Hainan University | Jiang X.,Hainan University | And 6 more authors.
Toxicology Letters | Year: 2016

Benzo(a)pyrene and dichlorodiphenyltrichloroethane are typical persistent organic pollutants, and also the widespread environmental estrogens with known toxicity towards green mussels Perna viridis. In this study, the toxicological effects of BaP and DDT and their mixture were assessed in green mussel gonads using proteomic and metabolomic approaches. Metabolomics by NMR spectroscopy revealed that BaP did not show obvious metabolite changes in the gonad of male green mussel. DDT mainly caused some disturbance of osmotic regulation and energy metabolism by changing BCAAs, alanine, threonine, arginine, etc., unknown metabolite (3.53. ppm), glycine, homarine and ATP at different levels. However, the mixture of BaP and DDT mainly caused some disturbance in osmotic regulation and energy metabolism by differentially altering branched chain amino acids, glutamate, alanine, arginine, unknown metabolite (3.53. ppm), glycine, 4-aminobutyrate, dimethylglycine, homarine and ATP. The results suggest that DDT alone may cause most of metabolites changes in the mixture exposed male mussel gonad, and the results also show that the male P. viridis gonad was more sensitive to DDT than BaP exposures. Proteomic study showed that BaP, DDT and their mixture may have different modes of action. Proteomic responses revealed that BaP induced signal transduction, oxidative stress, spermatogenesis, etc. in the male green mussel gonad; whereas DDT exposure altered proteins that were associated with signal transduction, oxidative stress, cytoskeleton and cell structure, cellular organization, energy metabolism, etc. However, the mixture of BaP and DDT affected proteins related to cytoskeleton and cell structure, oxidative stress, cellular organization, etc. This research demonstrated that metabolomic and proteomic approaches could better elucidate the underlying mechanism of environmental pollutants gonad toxicity. © 2015 Elsevier Ireland Ltd.


Wei L.,Hainan University | Li Y.,Hainan University | Qiu L.,Hainan University | Zhou H.,Hainan University | And 4 more authors.
Chemosphere | Year: 2016

Litopenaeus vannamei has a high commercial value and is the primary cultured shellfish species globally. In this study, we have compared the hemolymph physiological responses between two L. vannamei strains under acute hypoxia. The results showed that hemocyanin concentration (HC) of strain A6410 was significantly higher than strain Zhengda; Total hemocyte counts (THC) decreased significantly in both strains under hypoxic stress (p < 0.05). We also investigated the temporal and spatial variations of hypoxia inducible factors 1 (HIF-1) by qRT-PCR. The results showed that hypoxia for 12 h increased the expression levels of HIF-1α in tissues of muscle and gill from the two strains (p < 0.05). In the hepatopancreas, the expression levels of HIF-1 increased significantly in strain Zhengda and decreased significantly in strain A6410 (p < 0.05). No significant changes of HIF-1 expression were detected in the same tissues between the two strains under hypoxia for 6 h (p > 0.05), but in the gills and hepatopancreas under hypoxia for 12 h (p < 0.05). Additionally, the expression level of HIF-1 was higher in the strain Zhengda than A6410 in the same tissue under hypoxia for 12 h. It was indicated that the hypoxic tolerance of Litopenaeus vannamei was closely correlated with the expression level of HIF-1, and the higher expression level of HIF-1 to hypoxia, the lower tolerance to hypoxia in the early stage of hypoxia. These results can help to better understand the molecular mechanisms of hypoxic tolerance and speed up the selective breeding process of hypoxia tolerance in L. vannamei. © 2016 Elsevier Ltd.


Song Q.,Hainan University | Chen H.,Hainan University | Li Y.,Hainan University | Zhou H.,Hainan University | And 4 more authors.
Chemosphere | Year: 2016

Benzo(a)pyrene (BaP) and dichlorodiphenyltrichloroethane (DDT) are persistent organic pollutants and environmental estrogens (EEs) with known toxicity towards the green mussel, Perna viridis. In this study, the toxic effects of BaP (10 μg/L) and DDT (10 μg/L) and their mixture were assessed in green mussel gills with proteomic and metabolomic approaches. Metabolic responses indicated that BaP mainly caused disturbance in osmotic regulation by significantly decrease in branched chain amino acids, dimethylamine and dimethylglycine in gills of male green mussels after exposure for 7 days. DDT mainly caused disturbance in osmotic regulation and energy metabolism by differential alteration of betaine, dimethylamine, dimethylglycine, amino acids, and succinate in gills of male green mussels. However, the mixture of BaP and DDT didn't show obvious metabolite changes. Proteomic analysis showed different protein expression profiles between different treatment groups, which demonstrated that BaP, DDT and their mixture may have different modes of action. Proteomic responses revealed that BaP induced cell apoptosis, disturbance in protein digestion and energy metabolism in gills of green mussels, whereas DDT exposure altered proteins that were associated with oxidative stress, cytoskeleton and cell structure, protein digestion and energy metabolism. However, the mixture of BaP and DDT affected proteins related to the oxidative stress, cytoskeleton and cell structure, protein biosynthesis and modification, energy metabolism, growth and apoptosis. © 2015 Elsevier Ltd.


Li Y.,Hainan University | Li Y.,Haikou Key Laboratory of Environment Toxicology | Wei L.,Hainan University | Cao J.,Hainan Provincial Health School | And 7 more authors.
Chemosphere | Year: 2016

To evaluate the genotoxic and physiological effects of acute hypoxia on the pacific white shrimp (. L. vannamei), shrimps were treated firstly with three dissolved oxygen levels 6.5 ppm (control), 3.0 ppm and 1.5 ppm for 24 h, respectively, and then reoxygenated (6.5 ppm) for 24 h. The changes of superoxide dismutase (SOD) activity, glutathione peroxidases (GPX) activity, malondialdehyde (MDA) concentration and DNA damage in the tissues of gill, hepatopancreas and hemolymph were examined during the period of hypoxia and reoxygenation. The results indicated SOD activity, GPX activity, MDA concentration and DNA damage all increased basically compared with the control during the period of hypoxia except for MDA concentrations in the gill at 12 h and 24 h hypoxia (3.0 ppm), and these parameters were recovered to some degree during the period of reoxygenation. Moreover, the comet assays in the tissues of gill and hepatopancreas showed an obvious time- and dose-dependent response to hypoxia, which indicated comet assay in the two tissues could be used as sensitive biomarker to detect the occurrence of hypoxia. We conclude that acute hypoxia can induce oxidative stress, DNA damage and lipid peroxidation in the tissues of gill, hepatopancreas and hemolymph of L. vannamei and the DNA damage may come from hypoxia-induced oxidative stress. © 2015 Elsevier Ltd.


Li Y.,Hainan University | Liu H.,CAS Yantai Institute of Coastal Zone Research | Zhou H.,Hainan University | Zhou H.,CAS Yantai Institute of Coastal Zone Research | And 6 more authors.
Marine Pollution Bulletin | Year: 2015

To investigate the pollution level and evaluate the potential health risks of heavy metals, the concentrations of chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), selenium (Se), molybdenum (Mo), cadmium (Cd), antimony (Sb), and lead (Pb) were determined by inductively coupled plasma-mass spectrometry (ICP-MS) in 198 clams (Mactra veneriformis) collected from 11 sites of the Bohai Bay. The results showed that heavy metal concentrations in the clams were different at different sites (p<0.05). Mn was dominant with a percentage of 22.08-77.03% in heavy metals, followed by Zn with 12.66-57.11%, and the concentration of Pb was the lowest with 0.45-1.04%. The potential health risk to consumers was evaluated by the target hazard quotient (THQ) and the maximum daily consumption rate (CRmax). The results indicated that the THQs of Co were the highest with the values of 1.125, 1.665, and 1.144 at three sections; the values of other individual metals were <1, which indicated that consumption of clams from the study areas caused health risks due to Co. Moreover, the CRmax values also indicated the potential health risk caused by Co in clams consumed in this area. Pearson correlation analysis and principal component analysis (PCA) indicated that there were significantly positive or negative correlations between the heavy metals (p<0.05), and the studied metals were divided into four groups. The results indicated that the concentrations of heavy metals in clams were affected not only by pollution sources but also by the characteristics of clams that could absorb selectively and accumulate special metals. This study offers important information on the pollution levels of heavy metals in clams and warns consumers of the health risks associated with the consumption of clams in the area. © 2015 Elsevier Ltd.


Li P.,Hainan University | Li P.,Haikou Key Laboratory of Environment Toxicology | Diao X.,Hainan University | Diao X.,Haikou Key Laboratory of Environment Toxicology | And 12 more authors.
Marine Pollution Bulletin | Year: 2015

The study investigated the occurrence of polycyclic aromatic hydrocarbons (PAHs) in the surface sediment from eleven sites in Yangpu Bay, China in December 2013 (winter) and July 2014 (summer). The 16 US EPA priority PAHs were found in the range of 1583.2-5701.7 ng/g dry weights with an average of 3134.7 ± 1241.3 ng/g in winter and ranged from 2161.8 to 4527.2 ng/g with an average of 3016.6 ± 748.0 ng/g in summer, respectively. The concentrations of the PAHs tended to be relatively high in comparison with other areas from the literatures. The identification using molecular indices analysis indicated that the PAHs originated mainly from pyrogenic and petrogenic sources in most of the sites. According to principle component analysis-multiple linear regression (PCA/MLR) for their source apportionment, the main sources of PAHs were vehicle emissions, petroleum products and biomass combustion. The risk assessment using international sediments quality guidelines and sediments quality criteria indicated that several PAHs, such as Nap, Flu, Phe, Ace, Acy and BghiP in most of the sites would potentially affect organisms in Yangpu Bay. © 2015 Elsevier Ltd.


Li P.,Hainan University | Li P.,Haikou Key Laboratory of Environment Toxicology | Cao J.,Hainan University | Cao J.,Haikou Key Laboratory of Environment Toxicology | And 9 more authors.
Marine Pollution Bulletin | Year: 2015

The occurrence of 14 polycyclic aromatic hydrocarbons (PAHs) listed by the United States Environmental Protection Agency was investigated in surface seawater of Yangpu Bay, China in November 2013 (winter) and May 2014 (summer). Seventy-two samples were collected from 12 sampling sites. The total concentrations of PAHs in Yangpu Bay showed obvious variations in different seasons, which varied from 582.8 to 2208.3ngL-1 in winter and 952.4 to 1201.7 ng L-1 in summer, respectively. Two-ring PAHs accounted for more than 91.6% of total PAHs in winter, and three-ring PAHs were dominant with 81.6% of total PAHs in summer. Molecular indices analyses indicated that the main source of PAHs in Yangpu Bay could be petrogenic contamination. The ecological risk assessment by Risk quotients (RQNCs and RQMPCs) showed a potential ecological risk of PAHs in Yangpu Bay, indicating a close attention should be paid to pollution of PAHs in the coastal area of Yangpu. © 2015 Elsevier Ltd.


Li Y.,Hainan University | Li Y.,Haikou Key Laboratory of Environment Toxicology | Li P.,Haikou Key Laboratory of Environment Toxicology | Li P.,Hainan University | And 7 more authors.
Marine Pollution Bulletin | Year: 2015

Spatial and temporal distributions of 14 polycyclic aromatic hydrocarbons (PAHs) were investigated in surface waters of Haikou Bay, China from October 2013 to September 2014. The total PAHs concentrations ranged from 420.2 to 2539.1ngL-1 with the average value of 1016.3±455.8ngL-1, which were predominated by low molecular weight PAHs (2-and 3-ring PAHs). Moreover, PAHs displayed spatial and temporal variations in the concentration and composition pattern. Source analysis based on isomer ratios indicated that the PAHs mostly originated from petroleum and combustion processes. An eco-toxicological risk assessment showed that the potential risk of individual PAHs had reached moderate to high levels and the total concentrations of PAHs had also reached a relatively high level compared with previous studies. This study offers important information on the pollution levels of 14 PAHs in the surface waters of Haikou Bay and recommends that prevention and control of PAHs pollution should be implemented in the region. © 2014 Elsevier Ltd.


Qiu L.,Hainan University | Song Q.,Hainan University | Jiang X.,Hainan University | Zhao H.,Hainan University | And 7 more authors.
Metabolomics | Year: 2016

Introduction: Perna viridis is an ideal marine pollution bioindicator, benzo(a)pyrene (BaP) and 2,4′-dichlorodiphenyltrichloroethane (2,4′-DDT) are estrogenic and ubiquitous in marine environments. The toxic effects of a mixture of pollutants are more complicated. A global view of the protein and metabolite responses to single EEs compared to their mixture offers another dimension for revealing the toxic effects of EEs on marine animals. Objectives: The objective of this study was to investigate the different proteomic and metabolomic responses in the female gonad of the green mussel P. viridis exposed to BaP, 2,4′-DDT and their mixture. Methods: Here we have applied NMR spectroscopy-based metabolomics and two-dimensional gel electrophoresis mass spectrometry-based proteomics to elucidate the protein and metabolite responses of female green mussel gonad exposed to BaP, 2,4′-DDT and their mixture. The statistical analysis methods including partial least squares discriminant analysis and orthogonal projection to the latent structure with discriminant analysis were used for data classification. Results: Proteomic responses revealed that 2,4′-DDT and its mixture with BaP showed more severe disturbances in energy metabolism, cell structure and oxidative defense compared to BaP treatment alone. The results of the metabolic responses demonstrated that both 2,4′-DDT and its mixture with BaP mainly induced disturbances in energy metabolism and osmotic regulation. In contrast to what was found with the 2,4′-DDT treatment alone, the mixture of BaP and 2,4′-DDT mainly depressed taurine metabolism by lowering the expression of its metabolite, arginine. Furthermore, the differentially expressed proteins, pancreatic triacylglycerol lipase and the pyruvate dehydrogenase E1 component subunit beta, were confirmed by their related metabolic biomarkers, including ATP and acetoacetate. Conclusion: Our results provide an insight into the metabolic responses of mussel P.viridis exposed to either BaP, 2,4′-DDT or their mixture and characterize some potential biomarkers that were identified by a combined metabolomic and proteomic approach. © 2016, Springer Science+Business Media New York.

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