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Tianjin, China

Yu M.,Tianjin Medical University | Qiu N.-H.,Haihe Hospital
Chinese Journal of Tissue Engineering Research

BACKGROUND: The treatment of lumbosacral tuberculosis with multi-segmental vertebral destruction is complex. Besides, debridement and relief of spinal cord compression, it is necessary to restore spine vertebral body height and stability of the spine. OBJECTIVE: To investigate the surgical treatment effect of multi-segmental lumbosacral tuberculosis by posterior vertebral pedicle fixation and anterior debridement with autologous iliac bone graft. METHODS: A total of 25 cases of L2 -S2 vertebral tuberculosis with various degrees of damage, who were treated from March 2005 to December 2012, were selected in this study. After regular anti-tuberculosis treatment for 2-4 weeks, first-phase posterior pedicle screw fixation with anterior debridement and autologous iliac bone graft was performed. Postoperative X-ray and CT scan were conducted to assess bone fusion and deformity correction. Adverse events and material host reaction were recorded. RESULTS AND CONCLUSION: After repair, average 16-month follow-up was performed. 23 cases of postoperative wound were totally healed, with 2 cases of incisions delaying healing, no sinus formation. After surgery, the lumbago and leg pain disappeared in all patients. During follow-up, graft displacement had no slippage, broken nails or broken rods. Within 6 months, bony fusion occurred. At 1 year after operation, there was no recurrence of tuberculosis with normal erythrocyte sedimentation. X-ray films showed lesion vertebra turned out to be bony union. Postoperative lumbosacral angle was 16°-36°, averagely 26°, and 15°-30° during final follow-up, averagely 20°. These results confirmed that one-stage debridement for multi-segmental lumbosacral tuberculosis and concomitant posterior pedicle fixation system for kyphosis correction therapy, which could reconstruct lumbosacral stability, and restore sagittal plane balance. Autologous iliac bone graft improved fusion rate and obtained satisfactory outcomes. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

Fan Y.,Tianjin Medical University | Liu Y.,Childrens Hospital | Oklu R.,Massachusetts General Hospital | He N.-S.,Tianjin Medical University | And 5 more authors.
Diagnostic and Interventional Radiology

PURPOSE: Massive pulmonary embolism (PE) causes hemodynamic compromise and is associated with a high rate of mortality. We sought to create a model of massive PE and to determine whether occlusion of the right main bronchus could mitigate the physiological effects of massive PE in this model. MATERIALS AND METHODS: We used 27 female sheep to generate a model of massive PE by either autologous blood clot injection (n=18) or detachable balloon release (n=9) into the right main pulmonary artery. Four sheep were excluded after blood clot injection, as they did not exhibit adequate declines in blood oxygen saturation (SaO2). Nine of the sheep that received autologous blood clot and nine that received detachable balloons went on to treatment with right main bronchus occlusion. The control group (n=5) received the autologous blood clot, but no occlusion of the right main bronchus. All sheep underwent continuous monitoring of pulmonary arterial mean pressure (PAMP), SaO2, arterial partial pressure of oxygen (PaO2), and arterial partial pressure of carbon dioxide. RESULTS: Twenty-three sheep (85%) subjected to PE demonstrated immediate tachycardia, tachypnea, and decline in SaO2 of at least 25% within 30 min. After right main bronchus occlusion, 18 sheep (100%) survived for the length of the experiment and exhibited persistently higher SaO2 and PaO2 levels, as well as decreased PAMP compared with the controls. In the control group, two out of five sheep died within 30 min, and the three surviving subjects demonstrated significantly decreased SaO2 and PaO2 levels. CONCLUSION: Occlusion of the right main bronchus in an ovine model of massive PE effectively extends life and provides favorable physiological parameters to allow emergent interventions. © Turkish Society of Radiology 2012. Source

Liping W.,Nankai University | Liping W.,Tianjin University of Traditional Chinese Medicine | Weihua W.,Nankai University | Qian L.,Nankai University | And 4 more authors.
Macromolecular Symposia

Summary: glutaraldehyde cross-linked macroporous chitosan microspheres (CS) were prepared by inverse phase suspension reaction with sugar as porogenic agent. The microspheres were modified with different reagents of 1, 6 hexanediamine (HDA) and low generation polyamidoamine (PAMAM) dendrimers including PAMAM G1.0, PAMAM G2.0, PAMAM G3.0. The content of amino groups on CS, CS-PAMAM G1.0, CS-PAMAM G2.0, CS-PAMAM G3.0, CS-HDA was 3.56, 5.10, 5.47, 6.47, 4.66 mmol/g, respectively. The bilirubin adsorption on the above five microspheres was carried out in 0.05M phosphate buffer solution (pH = 7.2-7.4) at 37 °C. The results indicated all the modified CS microspheres were better than unmodified CS microspheres for bilirubin adsorption. CS-HDA has the best adsorption property even if the content of the amino groups was not very high. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Kim E.-Y.,University of Ulsan | Song H.-Y.,University of Ulsan | Kim J.H.,University of Ulsan | Fan Y.,Haihe Hospital | And 4 more authors.

Purpose: To investigate the efficacy of an IN-1233-eluting covered stent in preventing tissue hyperplasia in a rabbit esophageal model. Materials and Methods: The local animal research committee approved all experiments. Esophageal stents were placed in 40 male New Zealand rabbits (weight range, 2.8-3.2 kg). The drug group (D) received IN-1233-eluting covered stents (n = 20); the control group (C) received polyurethane-covered stents (n = 20). Drug loading of IN-1233-eluting covered stent was 10%. Four study groups were formed: C and D animals sacrificed at 4 (D4, C4) and 8 (D8, C8) weeks after stent placement (n = 10). Esophagography was used to assess the percentage of diameter stenosis. Histologic findings of the drug and control stents were compared. The Mann-Whitney U test was used to evaluate differences. Results: The mean percentage ± standard deviation of diameter stenosis was significantly lower in D groups than in C groups at both 4 (C4 = 36.15% ± 12.63, D4 = 7.83% ± 8.12 [P < .001]) and 8 (C8 = 50.21% ± 20.43, D8 = 27.78% ± 14.40 [P = .019]) weeks. Percentage of granulation tissue area (C4 = 33.07% ± 19.11, D4 = 21.59% ± 18.22 [P = .028]; C8 = 44.70% ± 21.71, D8 = 31.97% ± 22.54 [P = .131]), number of epithelial layers (C4 = 4.77 ± 1.55, D4 = 3.37 ± 1.73 [P = .002]; C8 = 5.50 ± 1.38, D8 = 4.50 ± 1.63 [P = .057]), and thickness of submucosal fibrosis (C4 = 2.42 mm ± 1.08, D4 = 1.62 mm ± 0.77 [P = .006]; C8 = 2.89 mm ± 1.00, D8 = 2.07 mm ± 0.71 [P = .007]) were lower in D than in C groups. Inflammatory cell infiltration was significantly higher in D than in C groups (C4 = 2.63 ± 0.81, D4 = 3.33 ± 1.09 [P = .032]; C8 = 2.20 ± 0.81, D8 = 3.00 ± 0.95 [P = .012]). Conclusion: The use of an IN-1233-eluting covered stents decreased tissue hyperplasia secondary to stent placement in a rabbit esophageal model. © RSNA, 2013. Source

Wu L.-P.,Nankai University | Wu L.-P.,Tianjin University of Traditional Chinese Medicine | Zhang Z.-P.,Nankai University | Guo Y.,Tianjin University of Traditional Chinese Medicine | And 2 more authors.
Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities

Glutaraldehyde cross-linked chitosan microspheres(CS) were prepared by inverse phase suspension reaction. Low generations(Gn, n≤3) hexanediamine-contained PAMAM dendrimers were supported to chitosan microspheres. Bilirubin adsorption on the above microspheres was carried out in 0.05 mol/L phosphate buffer solution(pH=7.2-7.4) at 37°C. The results show that the equilibrium adsorption percentage of CS-G2.0, CS-G3.0, CS-G1.0, CS-G0 and CS microspheres are 94.61%, 93.44%, 92.97%, 86.47% and 52.38%, respectively. The adsorption percentage of CS-G1.0-CS-G3.0 microspheres is over 70% at 0.5 h and arrived to the equilibrium at about 1 h. The adsorption capacity is up to 42.78 mg/g. All the moderations(CS-G0-CS-G3.0) are much better than CS microspheres for bilirubin adsorption. The effects of temperature, pH, bilirubin initial concentration, ion strength and bovine serum albumin(BSA) on the adsorption properties for bilirubin were studied. It was shown that adsorption percentage increased at higher temperature. Adsorption properties were the best at near neutral solutions of pH≈7. PAMAM dendrimers supported in chitosan microspheres had satisfactory competitive abilities with BSA for bilirubin adsorption though adsorption percentage decreaed to a certain extent in the presence of BSA. Source

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