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Wang J.,Tianjin Medical University | Liu H.,Haidian Maternal and Child Health Hospital | Qi Q.H.,Dalian Medical School
Hepato-Gastroenterology | Year: 2011

Background/Aims: The aim of this study was to investigate the effect of Da-Cheng-Qi-Tang (DCQT) on the gastrointestinal motility after abdominal surgery in humans. Methodology: Thirty-three patients with abdominal surgery and 36 patients with cholecystectomy were divided into DCQT and control groups at random. Electrogastrography (EGG) and antroduodenojejunal manometry were performed and the level of plasma ghrelin were measured by ELISA. Results: On the operative day, the ratio of EGG normal slow waves in DCQT group was higher than in control group (p<0.05). The power of EGG in DCQT group was higher than in control group on the 2 nd and 3 rd postoperative day (p<0.05). The percentage of normal bowel peristalsis was significantly higher in the DCQT group than in control group (p<0.01). On the 1 st and 2 nd postoperative day, the power of Phase III of migrating motor complex (MMC) in the proximal jejunum in DCQT group was significantly higher than in control group (p<0.05). On the 3 rd and 5 th postoperative day, the plasma ghrelin level in the DCQT group was significantly higher than in the control group (p<0.05). Conclusions: DCQT could improve gastric dysrythmia, enhance gastrointestinal motility and adjust the synchronized recovery of the alimentary tract and increase plasma ghrelin after abdominal surgery. © H.G.E. Update Medical Publishing S.A. Source

Qi W.,Peking University | Zhao C.,Peking University | Zhao L.,Peking University | Liu N.,Haidian Maternal and Child Health Hospital | And 3 more authors.
Cancer Cell International | Year: 2014

Background: Several reports have revealed that cancer stem cells (CSCs) exist in many types of solid tumors. Some studies have demonstrated that side population (SP) cells isolated from diverse cancer lines harbor cancer stem-like properties, but there are few reports examining the characteristic of SP cells in human cervical cancer. The aim of this study is 1) to find out a feasible way to detect the tumor stem-like cells in cervical cancer, and 2) to analyze the properties of the SP cells being sorted. Methods: Isolated SP and non-SP cells from human cervical cancer cell line Hela by Hoechst 33342 dying method and flow cytometry analysis. Observing morphology of SP and non-SP cells. The expression of various biomarkers putatively related to cancer stem cells were investigated by immucytochemistry of SP and non-SP cells. We also analyzed cell cycle and cell apoptosis for sorted cells. The oncogenicity of the SP and non-SP cells were analyzed by tumor formation in nonobesediabeti- c/severe combined immune- deficient (NOD/SCID) mice. The drug-resistant and radiation-resistant index between SP, non-SP and Hela cells was estimated by MTS assay. Results: The fraction of SP cells in Hela was approximately 1.07 ± 0.32%. SP cells were smaller and rounder in shape than non-SP cells, and mostly showed colony-like growth. Immunocytochemistry showed that stem cell makers (Oct3/4, CD133, BCRP) were highly expressed in SP cells. Moreover, the number of apoptotic cells among non-SP cells (17.6 ± 3.7%) was significantly higher compared with that among SP cells (4.4 ± 1.2%). The HE staining of in vivo grown tumors result from SP cells showed more poor differentiation, though no significant differences were shown between SP and non-SP cells in NOD/SCID mice tumorigenicity. Furthermore, SP cells demonstrated a higher degree of drug resistance against trichostatin A (TSA) compared with that of non-SP and Hela cells. SP cells were also found to be more resistant against radiotherapy. Conclusions: SP cells possess some characteristics of CSCs, namely high proliferation ability, chemoresistance and radioresistance, which may be helpful to elucidate novel targets for effective clinical treatments of cervical cancer in the future. © 2014 Qi et al.; licensee BioMed Central Ltd. Source

Teng Y.,Haidian Maternal and Child Health Hospital
Wei sheng yan jiu = Journal of hygiene research | Year: 2010

To investigate the effect of pre-pregnancy body mass index (BMI) on blood glucose, blood pressure and body weight during pregnancy in women. 600 pregnant women in Beijing Haidian Maternal and Child Health Hospital were followed from 5th, Feb. 2009 to 15th, Mar. 2009 as a cohort study. Height, weight, blood glucose and blood pressure were detected in all the pregnant period. The pre-pregnancy BMI correlated significantly with gestational weight in different pregnancy phases (P < 0.05). The rates of impaired glucose tolerance, diabetes mellitus and hypertension during pregnant in overweight and obesity women are much higher than the rates in low and normal body weight women (P < 0.05). The pre-pregnancy BMI is a crucial risk factor of gestational hypertension, gestational high blood glucose and the gestational body weight. To carry out pre-pregnancy health education is an important measure to prevent complications of pregnancy. Source

Wu J.-H.,Capital Medical University | Liang X.-A.,Haidian Maternal and Child Health Hospital | Wu Y.-M.,Capital Medical University | Li F.-S.,Capital Medical University | Dai Y.-M.,Capital Medical University
Oncology Reports | Year: 2013

The aim of the present study was to identify novel methylation markers for cervical cancer screening and to test the clinical application of the most promising biomarker in cervical scrapings. Methylated-CpG island recovery assay-based microarray analysis was carried out on a discovery set consisting of cervical cancer tissue and normal cervical tissue to identify significantly hypermethylated genes. Five hundred and four CpG islands, corresponding to 378 genes, were differentially methylated between cervical cancer tissue and normal cervical tissue. Among them, 30 genes were significantly hypermethylated. Of the 30 genes, SOX9, PKLR and DLX4 were selected for further validation by direct bisulfite sequencing. The SOX9 gene revealed complete methylation in the cervical cancer tissue and complete non-methylation in the normal control tissue. A TaqMan-based real-time PCR assay was performed to detect the methylation levels of the SOX9 gene in 156 cervical scrapings, including 48 normal cervical scrapings, 30 scrapings with cervical intraepithelial neoplasia 1 (CIN1), 30 scrapings with CIN2-3 and 48 scrapings with squamous cell carcinoma (SCC). The methylation levels (methylation score) of the SOX9 gene increased significantly with the severity of cervical squamous lesions. The area under the receiver operating characteristic (ROC) curve (AUC) revealed that the methylation score of the SOX9 gene could be used to segregate SCC/CIN2-3 from CIN1/normal (AUC, 0.961; P=0.000). At the optimal cut-off value, a sensitivity of 92.3% and a specificity of 89.7% were obtained. In conclusion, SOX9 methylation is frequently involved in cervical carcinogenesis, and may provide a valuable molecular biomarker for early detection of cervical cancer. Source

Zhang X.,Peking University | Zhang X.,National Institute of Occupational Health and Poison Control | Li J.,University of Wuppertal | Gu Y.,Haidian Maternal and Child Health Hospital | And 3 more authors.
Environmental Health and Preventive Medicine | Year: 2011

Objective The purpose of this study was to investigate the environmental and behavioral factors associated with the induction of missed abortion, with a particular focus on the relationship between job stress and missed abortion. Methods This was a case-control study in which 552 women participated (267 cases, 285 controls). Job stress was measured using the Job Content Questionnaire 1.0 (JCQ1.0). Results The case and control groups were significantly different for many factors, including age, physical exercise, exposure time to cell phone and computer, home refurbishment, ventilation, folic acid supplements, preference for fried food, reproductive knowledge, premarital health screening, parity, and supervisor support (P\0.05). For job stress, the univariate analysis revealed that there was no significant difference between cases and controls. Logistic analysis revealed that physical exercise (2-3 times per week or[3 times per week) [odds ratio (OR) 0.433, 95% confidence interval (CI) 0.213-0.881 or OR 0.268, 95% CI 0.106-0.680, respectively], ventilation (OR 0.415, 95% CI 0.251-0.685), folic acid supplements (OR 0.409, 95% CI 0.265-0.633), age (OR 1.102, 95% CI 1.033-1.174), and supervisor support (OR 0.870, 95% CI 0.768- .986) were the significant factors contributing to the missed abortion (P\0.05). Conclusions Physical exercise, ventilation, folic acid supplements, and supervisor support were identified as protective factors for the prevention of missed abortion. Advanced age at pregnancy was a risk factor for missed abortion. © The Japanese Society for Hygiene 2011. Source

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