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Huang C.,George Washington University | Nichols C.,George Washington University | Liu Y.,Emory University | Zhang Y.,Beijing Haidian Maternal and Child Health Hospital | And 4 more authors.
Population Health Metrics | Year: 2015

Background: Radical regulations to improve air quality, including traffic control, were implemented prior to and during the 2008 Beijing Olympic Games. Consequently, ambient concentrations of nitrogen dioxide (NO2) and particular matter 10 micrometers or less (PM10), were reduced in a distinct and short window of time, which presented a natural experiment for testing the relationships between maternal exposure to PM10 and NO2 during pregnancy and adverse birth outcomes. Methods: We estimated the effect of PM10 and NO2 exposure during each trimester of gestation on the risk of preterm birth among live births and the birth weight among term babies. The data were based on 50,874 live births delivered between January 1, 2006 and December 31, 2010 at the Beijing Haidian Maternal and Child Health Hospital. Air monitoring data for the same period were obtained from the Beijing Municipal Environmental Monitoring Center. Results: Among full-term births, maternal exposure to NO2 in the third trimester predicted birth weight, with each 10-unit increment (per 10 ug/m3) in NO2 concentration associated with a 13.78 g (95 % confidence interval: -21.12, -6.43; p < 0.0001) reduction in birth weight. This association was maintained after adjusting for other pollutants, including carbon monoxide (CO), sulfur dioxide (SO2), and PM10. No relationship was found between the concentration of PM10 and low birth weight among full-term births. Neither PM10 nor NO2 concentrations predicted the risk of premature birth. Conclusions: Exposure to ambient air pollution during certain periods of pregnancy may decrease birth weight, but the effect size is small. © 2015 Huang et al.


PubMed | Peking University and Beijing Haidian Maternal and Child Health Hospital
Type: Comparative Study | Journal: Reproduction, fertility, and development | Year: 2014

Y-chromosome microdeletions (YCMs) have been found at a much higher rate in infertile men than fertile controls. A specific deletion in the azoospermia factor locus (AZF) at Yq11 is significantly associated with male infertility. Whether assisted reproductive technology (ART) increases the risk of YCM in ART-derived offspring remains unclear. In this study the occurrence of YCM in 199 fathers and their 228 sons (Chinese, Han ethnicity), including 85 offspring conceived by IVF, 73 by intra-cytoplasmic sperm injection (ICSI) and 70 by natural conception, was investigated. Nineteen candidate genes related to YCM were analysed by multiplex ligation-dependent probe amplification. We identified one de novo YCM from 70 naturally-conceived offspring and none from 158 ART-conceived offspring and found no statistical significance between these two groups. There was no statistically-significant difference in the detection rate of the fathers Y-chromosome microdeletion group: IVF 10.7% (8/75), ICSI 3.2% (2/63), natural conception 8.2% (5/61). These results suggest that ART does not increase the risk of YCM in male offspring.


PubMed | Peking University and Beijing Haidian Maternal and Child Health Hospital
Type: Comparative Study | Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences | Year: 2014

To evaluate the relationship of incisal point displacements in the mandibular retruded contact position (RCP) between the self-controlled retruded approach and bimanual manipulation method.Twelve healthy young volunteers were selected. The RCP was guided through the self-controlled retruded approach and bimanual manipulation method. The track of the incisal point was recorded, using the mandibular movement trace recording system. The movement direction of the incisal point in horizontal plane was observed. The distance between the incisal point of the RCP and intercuspal contact position (ICP) was measured.Except one volunteers incisal point movement direction of the RCP was oblique, others were straight toward posterior. The displacements of the incisal point from the ICP to RCP guided through self-controlled approach and bimanual manipulation method were (1.15 0.64) mm, (0.98 0.29) mm respectively. There was no statistical significance between the two methods (P > 0.05).The displacements of the incisal point from the ICP to RCP guided through self-controlled approach and bimanual manipulation method are approximately the same.


Tu W.,Peking Union Medical College | He J.,Peking Union Medical College | Dai F.,Peking Union Medical College | Wang X.,Beijing Haidian Maternal and Child Health Hospital | Li Y.,Beijing Haidian Maternal and Child Health Hospital
Indian Journal of Pediatrics | Year: 2012

Objective: To estimate the incidence of Inborn errors of metobolism (IEM) in Neonatal intensive care unit (NICU) using tandem mass spectrometry and to determine the impact that these disorders have on NICU resources. Methods: During the period of study, 724 (81% eligible cases) dried blood filter-paper samples were collected from a NICU. The samples were analysed using tandem mass spectrometry. The diagnosis was further confirmed through clinical symptoms and by gas chromatographymass spectrometry. The results were also confirmed by clinical follow-up of all positive patients in an overall interval of 1 year. The mean observation period was 11 months per neonate. Results: In total, 22 cases were screen positive and 8 cases of inborn errors of metabolism were detected. The incidence of IEM in the population of patients admitted to the authors' NICU was 1.1%. The most common inborn error found was methylmalonic acidemia (3 cases, 37.5%), and all of the cases needed aggressive treatment and invasive mechanical ventilation. There were two cases of Tyrosinemia type 1, one case each of Maple Syrup Urine Disease, Propionic Acidemia, and Multiple Acyl-CoA dehydrogenase deficiency (MADD). Five of the eight patients required invasive mechanical ventilation. The median length of NICU stay was 3 days (1̃7 days) and early therapeutic intervention was effective for four of them and other four patients (50%) died. Conclusions: The incidence of IEM in NICU was 1.1%, indicating an underestimation of the incidence of metabolic disorders prior to implementing screening. Most patients with IEM in the NICU required invasive mechanical ventilation and the mortality was increased due to underlying IEM. © Dr. K C Chaudhuri Foundation 2011.


Tu W.-J.,Peking Union Medical College | He J.,Peking Union Medical College | Chen H.,China Rehabilitation Research Center | Shi X.-D.,China Rehabilitation Research Center | Li Y.,Beijing Haidian Maternal and Child Health Hospital
PLoS ONE | Year: 2012

Objectives: As more families participate expanded newborn screening for metabolic disorders in China, the overall number of false positives increases. Our goal was to assess the potential impact on parental stress, perceptions of the child's health, and family relationships. Methods: Parents of 49 infants with false-positive screening results for metabolic disorders in the expanded newborn screening panel were compared with parents of 42 children with normal screening results. Parents first completed structured interview using likert scales, closed and open questions. Parents also completed the parenting stress index. Results: A total of 88 mothers and 41 fathers were interviewed. More mothers in the false-positive group reported that their children required extra parental care (21%), compared with 5% of mothers in the normal-screened group (P<0.001). 39% of mothers in the false-positive group reported that they worry about their child's future development, compared with 10% of mothers in the normal-screened group (P<0.001). Fathers in the false-positive group did not differ from fathers in the normal-screened group in reporting worry about their child's extra care requirements, and their child's future development. Children with false-positive results compared with children with normal results were triple as likely to experience hospitalization (27%vs 9%, respectively; P<0.001). Conclusions: The results showing false-positive screening results may affect parental stress and the parent-child relationship. Parental stress and anxiety can be reduced with improved education and communication to parents about false-positive results. © 2012 Tu et al.


Gong L.-M.,Hospital of Zunyi Medical College | Tu W.-J.,Peking Union Medical College | He J.,Peking Union Medical College | Shi X.-D.,China Rehabilitation Research Center | And 2 more authors.
Journal of Bioethical Inquiry | Year: 2012

Objective: To investigate the attitudes of Chinese parents regarding the storage of dried blood spots collected for newborn screening (NBS) and their use in research. Methods: We conducted a hospital-based survey of parents and examined parental attitudes regarding (a) allowing NBS sample storage, (b) permitting use of children's NBS samples for research with parental permission, and (c) permitting use of children's NBS samples for research without parental permission. Results: The response rate was 52 percent. Of parents surveyed, 68 percent would permit their infant's NBS sample to be stored for at least some length of time. If permission is obtained, 69 percent of parents "strongly agreed" or "agreed" to permit use of the NBS sample for research. If permission is not obtained, only 14 percent of parents "strongly agreed" or "agreed." There was no significant association between permitting use of NBS samples for research and parental gender, education, household income, number of children, or site of residence. Conclusions: This is the first survey of Chinese parents regarding the use of NBS samples for different types of research, with results indicating that most parents would permit their infant's sample to be stored and would support the use of NBS dried blood spots for research purposes. © 2012 Springer Science+Business Media B.V.


Zhao Y.,Beijing Haidian Maternal and Child Health Hospital | Zhang Y.,Beijing Haidian Maternal and Child Health Hospital | Li Z.,Dalian Medical University
International Journal of Gynecology and Obstetrics | Year: 2014

Objective: To explore appropriate second-line therapies formanagement of severe postpartumhemorrhage at cesarean delivery.Methods: A retrospective study was done of 87 women who underwent cesarean delivery and received uterotonics after placental separation at the Beijing Haidian Maternal and Child Health Hospital, China, between 2009 and 2013. Group 1 (n=52) included patientswith 500700mL of blood loss before application of intrauterine gauze tamponade or B-Lynch suture as second-line therapy,while group 2 (n=35) included patientswith blood loss ofmore than 700 mL before application of either gauze tamponade or B-Lynch suture.Results: Managementwas successful in all patients in group 1. In group 2, additional management was needed in three of four patientswho underwent a B-lynch suture. Factors significantly associatedwith total blood losswere blood loss before application of second-line therapy (P < 0.001), fibrinogen levels (P < 0.001), and time from placental separation to second-line therapy (P= 0.015).Conclusion: When blood loss is 500700 mL, compression sutures or intrauterine gauze tamponade can be used as second-line treatment of postpartum hemorrhage. When blood loss is more than 700 mL, intrauterine gauze tamponade should be used. © 2014 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.


Zhang F.,Liaoning Medical University | Zhang F.,Beijing Haidian Maternal and Child Health Hospital | Liu J.,Liaoning Medical University | Lin B.,Liaoning Medical University | And 6 more authors.
International Journal of Molecular Sciences | Year: 2011

Epithelial carcinomas of the ovary exhibit the highest mortality rate among gynecologic malignancies. Studies found that the metabolism of glycolipids or carbohydrates is associated with acquirement of anticancer drug-resistance by cancer cells. This study was to characterize possible involvement of Lewis Y (Le Y) antigen in the drug-resistance of cancer cells. We transfected the α1,2-fucosyltransferase gene into human ovarian carcinoma-derived RMG-1 cells and established RMG-1-hFUT cells with enhanced expression of Le Y. We determined the effects of docetaxel on the survival of cells by MTT assaying and observed the apoptosis of cells in the presence of docetaxel by flow cytometric analysis and by transmission electron microscopy. Plasma membranes and intracellular granules in RMG-1-hFUT cells were stained with anti-Le Y antibody, the intensity of the staining was higher than that in control cells. The RMG-1-hFUT cells exhibited higher resistance to docetaxel than the control cells with regard to the docetaxel concentration and time course. After treatment with 10 μg/mL docetaxel for 72 h, thecontrol ells, but not RMG-1-hFUT, contained abundant positively stained cell debris dueto disintegration of the cytoskeleton. On transmission electron microscopy, although thecontrol cells treated with docetaxel as above showed the following morphology, i.e., absence of villi, cells shrunken in size, pyknosis, agglutinated chromatin and cell buds containing nuclei in the process of apoptosis, the RMG-1-hFUT cells showed only agglutinated chromatin and vacuoles in the cytoplasm. In summary, cells with enhanced expression of Le Y were shown to acquire docetaxel-resistance, indicating the possible involvement of glycoconjugates in the drug-resistance. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


Xu Y.,Capital Medical University | Chen W.,Beijing Haidian Maternal and Child Health Hospital | Lu H.,Capital Medical University | Hu X.,Capital Medical University | And 3 more authors.
Molecular Vision | Year: 2010

Purpose: The cytokines present in the aqueous humor and serum of C3H/HeN mice with endotoxin-induced acute anterior uveitis were analyzed, and the potential role of the cytokines in the pathogenesis of the disease was investigated. Methods: One hundred and eighty C3H/HeN mice were divided into an experimental group (n=150) and a control group (n=30). The mice in the experimental group were footpad-injected with 200 μg Vibro cholerae endotoxin (classical biotype, serotype Ogawa). The mice were then executed 4 h, 8 h, 16 h, 24 h, and 48 h after the injection of endotoxin. Aqueous humor and peripheral blood samples were collected using a microinjector. Ten samples were pooled together for analysis and centrifuged at 705× g, at 4°C, for 3 min. The supernatant was collected and stored at -80°C. The concentrations of tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-γ (IFN-γ) in the samples were measured using a Cytometric Bead Array (CBA). Results: Acute anterior uveitis was successfully induced in C3H/HeN mice. At roughly 16 h post-injection, the concentrations of both IL-1 and IL-6 reached peak levels, and were significantly different from the control group (p=0.001 and p=0.001, respectively). At 24 h post-injection, the concentrations of IFN-γ and IL-10 in the aqueous humor reached peak levels, and were significantly different from the control group (p=0.022 and p=0.003). The concentrations of IFN-γ in serum at 4 and 24 hours were significantly different from the control group (p=0.033 and p=0.032). The concentration of IL-10 in serum, at 24 h post-injection, was also found to be significantly different from the control group (p=0.003). The cytokine expression levels in the aqueous humor were consistent with what would be expected during the process of inflammation. Conclusions: Both IL-1 and IL-6 appear to play an important role in acute anterior uveitis; furthermore, the severity of inflammation may be associated with the dynamic balance of IFN-γ and IL-10. Our results suggest that the cytokine network might be a useful therapeutic target in the treatment of acute anterior uveitis. © 2010 Molecular Vision.


PubMed | Dalian Medical University and Beijing Haidian Maternal and Child Health Hospital
Type: Journal Article | Journal: International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics | Year: 2014

To explore appropriate second-line therapies for management of severe postpartum hemorrhage at cesarean delivery.A retrospective study was done of 87 women who underwent cesarean delivery and received uterotonics after placental separation at the Beijing Haidian Maternal and Child Health Hospital, China, between 2009 and 2013. Group 1 (n=52) included patients with 500-700 mL of blood loss before application of intrauterine gauze tamponade or B-Lynch suture as second-line therapy, while group 2 (n=35) included patients with blood loss of more than 700 mL before application of either gauze tamponade or B-Lynch suture.Management was successful in all patients in group 1. In group 2, additional management was needed in three of four patients who underwent a B-lynch suture. Factors significantly associated with total blood loss were blood loss before application of second-line therapy (P<0.001), fibrinogen levels (P<0.001), and time from placental separation to second-line therapy (P=0.015).When blood loss is 500-700 mL, compression sutures or intrauterine gauze tamponade can be used as second-line treatment of postpartum hemorrhage. When blood loss is more than 700 mL, intrauterine gauze tamponade should be used.

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