Chen F.,General Hospital of Beijing Military Command |
Chen F.,Haidian Hospital |
Duan Y.,General Hospital of Beijing Military Command |
Li Y.,General Hospital of Beijing Military Command |
And 5 more authors.
International Journal of Surgery | Year: 2017
Background Epilepsy is a common complication of cranioplasty. The present study was designed to explore the clinical effect of prophylactic anti-epilepsy drugs (AED) to control epileptic seizures associated with cranioplasty. Methods and design: This trial was a prospective, randomized, open-label, single-centre, active controlled study designed to investigate the use of antiepileptic drug to control epileptic seizures associated with cranioplasty. We tested the necessity and methods of drug use. Three hundred twenty epilepsy patients who underwent cranioplasty were included in this study. The patients were randomly divided into the control group (160 cases) and the experimental group (160 cases). AED were administered to experimental group from 4 days before the surgery until 1 month after the surgery. The incidence of early and late epileptic seizures after cranioplasty was analyzed. The liver function, abnormal blood test 1 month after surgery were compared between these two groups. Results The incidence of seizures in the Control group was 28.6% (43 cases in 149 cases) while in the experimental group was only 5.9% (9 cases in 151 cases), which had statistical significance. The incidence of epileptic seizure was significantly higher in patients who received no AED treatment than in those who received AED treatment. Besides, the abnormal liver function and blood routine examination in both control and experimental group had no significant differences. Conclusion The incidence of epilepsy associated with the cranioplasty is high and early use of anti-epileptic drugs can effectively reduce the occurrence of seizures. © 2017 IJS Publishing Group Ltd
Jiang T.,University of Houston |
Ince N.F.,University of Houston |
Jiang T.,Tian Tan Hospital |
Wang T.,Haidian Hospital |
And 4 more authors.
International IEEE/EMBS Conference on Neural Engineering, NER | Year: 2015
High density electrocorticogram (ECoG) of motor cortex was recorded during awake surgeries of two subjects with respectively epilepsy and brain tumor. Subjects were asked to execute spontaneous hand movements: extension and flexion during the experiments. We investigated the ECoG in time frequency plane during these hand movements and computed event-related desynchronization (ERD) and event-related synchronization (ERS) levels of beta (8-32 Hz) and gamma frequency bands. The distribution of ERD and ERS over channels provided an idea about the spatial distribution of the cortical activity within each task. In both subjects, consistently, the scale of power changes in beta and gamma band was larger in hand flexion compared to hand extension. In addition, the spatial distribution of ERD in beta and ERS in gamma band during flexion was larger than extension but highly overlapped between these tasks. Gamma band was more spatially localized than beta band activity and cortical patterns were more distinct between the tasks. Our preliminary results indicate that the high density ECoG can be effectively used in awake surgery for functional mapping. © 2015 IEEE.
Jiang T.,University of Houston |
Ince N.F.,University of Houston |
Jiang T.,Tian Tan Hospital |
Wang T.,Haidian Hospital |
And 3 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2015
We recorded motor cortical activity using highdensity electrocorticogram (ECoG) from three patients during awake craniotomy. Subjects repeatedly executed hand flexion/extension tasks according to auditory instructions. Clear event-related desynchronization (ERD) in beta band (8-32) Hz and event-related synchronization (ERS) in gamma band (60-200) Hz were observed. High frequency band (HFB: 60-200 Hz) activation was found to be more localized compared to low frequency band (LFB: 8-32 Hz) activation in all subjects. Local spatial correlation maps in LFB and HFB were constructed by computing the correlation between channels. Local spatial correlation dropped more in the ERD/ERS areas consistently in two subjects. The results indicate that ERD/ERS patterns are more spatially uncorrelated and denser ECoG electrode is necessary within these areas to map uncorrelated 'sources'. High resolution electrodes might improve both clinical functional mapping and brain machine interface outcomes in the near future. © 2015 IEEE.
Wang L.,Harbin Medical University |
Yu C.-J.,Harbin Medical University |
Liu W.,Haidian Hospital |
Cheng L.-Y.,Harbin Medical University |
Zhang Y.-N.,Harbin Medical University
Acta Pharmacologica Sinica | Year: 2011
Aim: To investigate the protective effects of rosiglitazone (RGZ) against the neuronal toxicity induced by advanced glycation end products (AGEs) and the underlying mechanisms. Methods: Neuroblastoma cell line SH-SY5Y was used. Cell viability and apoptosis were assessed using MTT assay and flow cytometry, respectively. Superoxide dismutase (SOD) and catalase activities were measured using biochemical methods. Intracellular reactive oxygen species (ROS) were monitored using 2',7'-dichlorodihydro-fluorescein diacetate (DCFH-DA). Secreted β-amyloid 1-42 (Aβ 1-42) level was assessed by ELISA. The expression of mRNA of Bcl2, Bax, Caspase3, Aβ precursor protein (APP), β-site APP-cleaving enzyme 1 (BACE1), and insulin degrading enzyme (IDE) were measured using quantitative real-time PCR (Q-PCR), and their protein levels were examined using Western blot. Results: RGZ (0.1-10 μmol/L) significantly increased the cell viability that was reduced by AGEs (1000 μg/mL). RGZ (10 μmol/L) significantly ameliorated AGEs-triggered downregulation of SOD and catalase, and production of ROS. It also reversed Bcl2 downregulation, Bax upregulation and Caspase3 expression caused by AGEs. Moreover, it significantly attenuated AGEs-induced Aβ secretion and APP protein upregulation. RGZ did not affect BACE1 expression, but induced IDE expression, which promoted degradation of Aβ. All the effects were blocked by the specific PPARγ antagonist GW9662 (10 μmol/L). Conclusion: RGZ protects the euroblastoma cells against AGEs-induced injury via its anti-oxidative, anti-apoptotic and anti-inflammatory properties that seems to be mediated by PPARγ activation. The results suggest a beneficial role for RGZ in the treatment of Alzheimer's disease. © 2011 CPS and SIMM All rights reserved.
Ma Y.-D.,Chinese PLA General Hospital |
Ma Y.-D.,Nankai University |
Wang J.,Chinese PLA General Hospital |
Du Z.-H.,Haidian Hospital |
And 3 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2014
BACKGROUND: In acute ischemic stroke patients, internal carotid artery occlusion with middle cerebral artery (ICA/MCA) occlusion in succession predicts a poor outcome after systemic thrombolysis. It is not known whether this occlusion subtype of the anterior circulation is due to dissections or cardiogenic thromboembolism. We aimed to find useful evidence to judge the condition with accuracy and establish reasonable treatment protocols. PATIENTS AND METHODS: This retrospective study included 7 consecutive patients with acute ICA/MCA occlusion in succession who had undergone mechanical thrombectomy with a Solitaire stent retrieval between January 2012 and June 2013. Then we also reviewed the current literature. RESULTS: The patients had a mean age of 56 years and a mean baseline National Institutes of Health Stroke Scale (NIHSS) score of 20. The procedure resulted in thrombolysis in cerebral ischemia (TICI) scores of 2a or better in all patients, but complete recanalization of the ICA occlusion segment was achieved in only 2 patients. Stenting was not performed in all patients. At 90 days, 1 patient was dead and 4 of the 7 patients had favorable functional outcomes (modified Rankin score (mRS) ≥ 2). We identified 9 studies with 85 patients with nonath-erosclerotic acute ICA occlusion who underwent mechanical thrombectomy with Solitaire stent. The mean age was 65 years with a mean baseline National Institute Health Stroke Scale (NIHSS) score of 16 and mean time to treatment of 242 minutes. The mean time of the procedures ranged from 40-160 minutes in 9 studies. Successful recanalization was achieved in 69.4% of the patients and mortality was 16.5%. Favorable outcome (mRS ≤ 2) occurred in 42.4% of patients. Few studies stated whether complete recanalization was achieved in patients with ICA occlusion. CONCLUSIONS: Our results and the literature review suggest that mechanical thrombectomy in acute stroke due to ICA/MCA occlusion is feasible and safe, with high rates of recanalization and favorable functional outcomes. More patients with ICA/MCA occlusion in succession could obtain favorable functional outcomes with accurate judgment of the lesion location and appropriate treatment protocols. However, there is no consensus on how to judge the correct location of the ICA dissected portion and whether stenting is appropriate.
Yu L.,Haidian Hospital |
Sun L.,Coal General Hospital |
Chen S.,China Aerospace Science and Technology Corporation
Experimental and Therapeutic Medicine | Year: 2014
Postoperative cognitive dysfunction (POCD) is common in elderly patients. Senegenin, an active component of extracts from Polygala tenuifolia root, a traditional Chinese medicine, has neuroprotective and neuroregenerative effects. However, the mechanism underlying the effects of senegenin against postoperative cognitive impairment in elderly individuals has yet to be elucidated. The aim of this study was to investigate the protective effects of senegenin on the cognitive functions of elderly rats with splenectomy-induced POCD. Results from a Morris water maze test suggested that splenectomy induced a transient cognitive deficiency in the elderly rats; however, when the rats were treated with senegenin, the cognitive impairment was notably attenuated. Further experiments showed that senegenin significantly inhibited the mRNA and protein expression of several key pro-inflammatory cytokines, specifically, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-8, in the hippocampal tissues of elderly rats following splenectomy. In order to investigate the molecular mechanism involved, the expression and activity of the Toll-like receptor 4 (TLR4) signaling pathway was assessed. On day 1 postoperatively, it was observed that senegenin markedly suppressed the mRNA and protein expression of TLR4, myeloid differentiation factor 88 (MyD88) and TIR domain-containing adaptor-inducing interferon-β (TRIF). Furthermore, the phosphorylation levels of nuclear factor-κB (NF-κB) p65 and inhibitor of NF-κB (IκBα) were also decreased following senegenin treatment on the first day subsequent to surgery. These results suggest that senegenin suppressed splenectomy-induced transient cognitive impairment in elderly rats, possibly by downregulating two signaling pathways involved in inflammation, TLR4/MyD88/NF-κB and TLR4/TRIF/NF-κB, to further inhibit the expression of key pro-inflammatory cytokines, specifically, TNF-α, IL-1β, IL-6 and IL-8, and ultimately the neuroinflammation in the hippocampal tissues. In conclusion, the present study revealed that senegenin exhibited neuroprotective effects against splenectomy-induced transient cognitive impairment in elderly rats, which indicated that senegenin may be a promising agent for the treatment of POCD.
Guo X.,Capital Medical University |
Yang W.,Haidian Hospital |
Ni J.,Capital Medical University |
He M.,Capital Medical University |
Yang L.,Capital Medical University
European Journal of Nutrition | Year: 2011
Purpose Catch-up growth is always companied with later development of obesity and osteoporosis that are two interrelated clinical entities. However, the potential mechanism of the link between them during catch-up growth is unknown. Methods Rats were divided into two groups. Rats of the normal control (NC) group were offered ad libitum access to food, while rats of CUGFR group were food restricted for 4 weeks, and then were allowed full access to food for 0, 2, 4 weeks, respectively. The fat percentage and distribution, bone mineral density, biochemical and histological indexes of bone were detected. Moreover, the expression of adipogenic or osteoblastic differentiation-related genes of mesenchymal stem cells (MSCs) was also determined. Results Catch-up growth led to a rapid visceral fat accumulation. Although there was no difference in the histological indexes of bone between NC group and CUGFR group, the bone turnover marker, serum Bone Gla-protein (s-BGP), decreased in CUGFR group. The adipogenic differentiation-related gene of MSCs, PPARgamma, was significantly higher than that of NC group especially when catch-up growth for 4 weeks. Nevertheless, the osteoblastic differentiation-related gene of MSCs, Runx2, was increased but failed to reach the levels of the controls eventually. Both protein and mRNA of TAZ, a main transcriptional modulator of MSCs differentiation, failed to catch up even after being allowed full access to food for 4 weeks. Conclusion CUGFR induces the differential differentiation of MSCs, potentially suppressing bone formation and favoring catch-up fat, which might be responsible for the increased risk of osteoporosis and obesity during CUGFR. © Springer-Verlag 2011.
Luo J.,Chinese PLA General Hospital |
Luo J.,Nankai University |
Zhao Q.,Nankai University |
Zhang W.,Chinese PLA General Hospital |
And 5 more authors.
Molecular Medicine Reports | Year: 2014
Breast cancer has a high fatality rate. Early diagnosis reduces the rate of mortality; therefore, novel diagnostic methods are urgently required. The present study investigated the correlation of serum microRNA (miRNA/miR) expression with breast cancer, and tested a panel of miRNAs as promising potential biomarkers for breast cancer. Six miRNAs (miR-374, miR-666-5p, miR-451, miR-148a, miR-27a and miR-30b) were selected for analysis and their differential expression levels were quantified using qPCR. The results demonstrated that four out of the six candidate miRNAs were significantly downregulated in breast cancer patients (miR-451, P=0.000; miR-148a, P=0.021; miR-27a, P=0.013 and miR-30b, P=0.001). A panel of miRNAs consisting of the four downregulated miRNAs was able to distinguish breast cancer from healthy controls, with an area under the receiver operating characteristic curve of 95.3%, a sensitivity of 94.7% and a specificity of 82.8%. Thus, this panel of miRNAs may be used as a sensitive and specific tool for the diagnosis of breast cancer.
Chen L.-L.,Huazhong University of Science and Technology |
Yang W.-H.,Huazhong University of Science and Technology |
Yang W.-H.,Haidian Hospital |
Zheng J.,Huazhong University of Science and Technology |
And 2 more authors.
Nutrition | Year: 2011
Objective: The purpose of this study was to examine how catch-up growth modulated islet function and what the detailed mechanisms were, especially at various stages and in different forms, of catch-up growth. Methods: We examined the modulation of islet function during catch-up growth by employing an oral glucose tolerance test and gained some insight into the possible mechanisms involved by measuring general physiologic parameters, pancreatic morphometry, insulin content, and the state of chronic oxidative stress. Correlation analyses were used to assess the correlation of insulin/glucose incremental area ratio to other parameters. Results: The catch-up growth groups resulted in damage to islet function as shown by an increased insulin/glucose incremental area ratio (P ≤ 0.05), smaller relative area of β-cells (P ≤ 0.05), larger relative area of α-cells (P ≤ 0.05), lower insulin content (P ≤ 0.05), increased nitric oxide and malondialdehyde concentrations, and decreased superoxide dismutase concentration (P ≤ 0.05, respectively). With time these changes became increasingly unmarked. Conclusion: Catch-up growth in different stages and forms induces varying degrees of islet dysfunction, possibly by corresponding changes in general physiologic parameters, pancreatic morphometry, insulin content, and the state of chronic oxidative stress. © 2011 Elsevier Inc.
PubMed | Haidian Hospital, Cixian Tumor Hospital and Academy of Military Medical Science
Type: Journal Article | Journal: Gene | Year: 2015
Ribosomal protein L34 (RPL34) was reported to be involved in the regulation of cell proliferation of prokaryotes, plant and animal cells. In the present study, we analyze the expression and function of RPL34 in NSCLC. Immunohistochemical analysis, qPCR and Western blot were used to detect the expression of RPL34 in NSCLC tissues and cells lines. Flow cytometry was used to detect cell activity of NSCLC cell line H1299 under lentivirus-mediated RNAi on RPL34. Cell proliferation and colony formation assays were used to analyze the role of RPL34 in NSCLC cell proliferation. We found that expression of ribosomal protein RPL34 was significantly up-regulated in NSCLC tissues compared to adjacent normal tissues. Lentivirus-mediated shRNA knockdown of RPL34 in NSCLC cell line H1299 resulted in a strong decrease of proliferation, and a moderate but significant increase of apoptosis and S-phase arrest. These data indicate that over-expressed RPL34 may promote malignant proliferation of NSCLC cells, thus playing an important role in development and progress of NSCLC.