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Haidian, China

Wang L.,Harbin Medical University | Yu C.-J.,Harbin Medical University | Liu W.,Haidian Hospital | Cheng L.-Y.,Harbin Medical University | Zhang Y.-N.,Harbin Medical University
Acta Pharmacologica Sinica | Year: 2011

Aim: To investigate the protective effects of rosiglitazone (RGZ) against the neuronal toxicity induced by advanced glycation end products (AGEs) and the underlying mechanisms. Methods: Neuroblastoma cell line SH-SY5Y was used. Cell viability and apoptosis were assessed using MTT assay and flow cytometry, respectively. Superoxide dismutase (SOD) and catalase activities were measured using biochemical methods. Intracellular reactive oxygen species (ROS) were monitored using 2',7'-dichlorodihydro-fluorescein diacetate (DCFH-DA). Secreted β-amyloid 1-42 (Aβ 1-42) level was assessed by ELISA. The expression of mRNA of Bcl2, Bax, Caspase3, Aβ precursor protein (APP), β-site APP-cleaving enzyme 1 (BACE1), and insulin degrading enzyme (IDE) were measured using quantitative real-time PCR (Q-PCR), and their protein levels were examined using Western blot. Results: RGZ (0.1-10 μmol/L) significantly increased the cell viability that was reduced by AGEs (1000 μg/mL). RGZ (10 μmol/L) significantly ameliorated AGEs-triggered downregulation of SOD and catalase, and production of ROS. It also reversed Bcl2 downregulation, Bax upregulation and Caspase3 expression caused by AGEs. Moreover, it significantly attenuated AGEs-induced Aβ secretion and APP protein upregulation. RGZ did not affect BACE1 expression, but induced IDE expression, which promoted degradation of Aβ. All the effects were blocked by the specific PPARγ antagonist GW9662 (10 μmol/L). Conclusion: RGZ protects the euroblastoma cells against AGEs-induced injury via its anti-oxidative, anti-apoptotic and anti-inflammatory properties that seems to be mediated by PPARγ activation. The results suggest a beneficial role for RGZ in the treatment of Alzheimer's disease. © 2011 CPS and SIMM All rights reserved. Source

Ma Y.-D.,Chinese PLA General Hospital | Ma Y.-D.,Nankai University | Wang J.,Chinese PLA General Hospital | Du Z.-H.,Haidian Hospital | And 3 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2014

BACKGROUND: In acute ischemic stroke patients, internal carotid artery occlusion with middle cerebral artery (ICA/MCA) occlusion in succession predicts a poor outcome after systemic thrombolysis. It is not known whether this occlusion subtype of the anterior circulation is due to dissections or cardiogenic thromboembolism. We aimed to find useful evidence to judge the condition with accuracy and establish reasonable treatment protocols. PATIENTS AND METHODS: This retrospective study included 7 consecutive patients with acute ICA/MCA occlusion in succession who had undergone mechanical thrombectomy with a Solitaire stent retrieval between January 2012 and June 2013. Then we also reviewed the current literature. RESULTS: The patients had a mean age of 56 years and a mean baseline National Institutes of Health Stroke Scale (NIHSS) score of 20. The procedure resulted in thrombolysis in cerebral ischemia (TICI) scores of 2a or better in all patients, but complete recanalization of the ICA occlusion segment was achieved in only 2 patients. Stenting was not performed in all patients. At 90 days, 1 patient was dead and 4 of the 7 patients had favorable functional outcomes (modified Rankin score (mRS) ≥ 2). We identified 9 studies with 85 patients with nonath-erosclerotic acute ICA occlusion who underwent mechanical thrombectomy with Solitaire stent. The mean age was 65 years with a mean baseline National Institute Health Stroke Scale (NIHSS) score of 16 and mean time to treatment of 242 minutes. The mean time of the procedures ranged from 40-160 minutes in 9 studies. Successful recanalization was achieved in 69.4% of the patients and mortality was 16.5%. Favorable outcome (mRS ≤ 2) occurred in 42.4% of patients. Few studies stated whether complete recanalization was achieved in patients with ICA occlusion. CONCLUSIONS: Our results and the literature review suggest that mechanical thrombectomy in acute stroke due to ICA/MCA occlusion is feasible and safe, with high rates of recanalization and favorable functional outcomes. More patients with ICA/MCA occlusion in succession could obtain favorable functional outcomes with accurate judgment of the lesion location and appropriate treatment protocols. However, there is no consensus on how to judge the correct location of the ICA dissected portion and whether stenting is appropriate. Source

Li X.-B.,Peking Union Medical College | Shi L.,Haidian Hospital | Zhang S.-M.,Peking Union Medical College
Chinese Journal of Cancer Research | Year: 2010

Objective: Anorectal malignant melanoma (AMM) is a rare and aggressive malignant tumor, and its treatment still controversial. This study was to investigate and summarize our experience on diagnosis, treatment and misdiagnosis of AMM. Methods: From August 1980 to December 2009, 42 patients with AMM were treated in our hospital. The clinical data of those patients were retrospectively analyzed, including the major symptoms, treatment and prognosis. Further immunophenotyping analyses using antibodies to S-100 protein, HMB-45 reactive antigen and vimentin were performed in 22 specimens. Results: The major complaints among the 42 cases were hematochezia (23/42, 55.0%), anal masses (12/42, 28.6%), changes in stool character (4/42, 9.5%), and anal tenesmus or pain (3/42, 7.1%). The misdiagnosis rate was 62% (26/42), the most common misdiagnosis consisted of hemorrhoid (9/26, 34.6%), polyps (11/26, 42.3%) and rectal cancer (6/26, 23.1%). 28 cases underwent abdominoperineal resection, 10 underwent local wide excision, 3 underwent palliative operation, and 1 with no operation. After surgery, 15 patients underwent radiotherapy, 21 underwent chemotherapy and 8 were treated with both. 22 cases deceased within 1 year, 11 within 2 years, 5 within 3 years and 1 within 4 years. The median survival time was 11 months, with the longest of 45 months. The immunohistochemical findings revealed that the S-100 protein was present in all 22 cases (100%), HMB-45 was stained in 19 of 22 (86.4%) and vimentin in 21 of 22 (95.5%). Conclusion: AMM is a rare disease with a poor prognosis and with an aptitude of being misdiagnosed. Hematochezia is the most common symptom. Immunohistochemical staining is conducive to the diagnosis of AMM. Operation is the major treatment, the operation type should be individualized. © 2010 Chinese Anti-Cancer Association and Springer Berlin Heidelberg. Source

Yu L.,Haidian Hospital | Sun L.,Coal General Hospital | Chen S.,China Aerospace Science and Technology Corporation
Experimental and Therapeutic Medicine | Year: 2014

Postoperative cognitive dysfunction (POCD) is common in elderly patients. Senegenin, an active component of extracts from Polygala tenuifolia root, a traditional Chinese medicine, has neuroprotective and neuroregenerative effects. However, the mechanism underlying the effects of senegenin against postoperative cognitive impairment in elderly individuals has yet to be elucidated. The aim of this study was to investigate the protective effects of senegenin on the cognitive functions of elderly rats with splenectomy-induced POCD. Results from a Morris water maze test suggested that splenectomy induced a transient cognitive deficiency in the elderly rats; however, when the rats were treated with senegenin, the cognitive impairment was notably attenuated. Further experiments showed that senegenin significantly inhibited the mRNA and protein expression of several key pro-inflammatory cytokines, specifically, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-8, in the hippocampal tissues of elderly rats following splenectomy. In order to investigate the molecular mechanism involved, the expression and activity of the Toll-like receptor 4 (TLR4) signaling pathway was assessed. On day 1 postoperatively, it was observed that senegenin markedly suppressed the mRNA and protein expression of TLR4, myeloid differentiation factor 88 (MyD88) and TIR domain-containing adaptor-inducing interferon-β (TRIF). Furthermore, the phosphorylation levels of nuclear factor-κB (NF-κB) p65 and inhibitor of NF-κB (IκBα) were also decreased following senegenin treatment on the first day subsequent to surgery. These results suggest that senegenin suppressed splenectomy-induced transient cognitive impairment in elderly rats, possibly by downregulating two signaling pathways involved in inflammation, TLR4/MyD88/NF-κB and TLR4/TRIF/NF-κB, to further inhibit the expression of key pro-inflammatory cytokines, specifically, TNF-α, IL-1β, IL-6 and IL-8, and ultimately the neuroinflammation in the hippocampal tissues. In conclusion, the present study revealed that senegenin exhibited neuroprotective effects against splenectomy-induced transient cognitive impairment in elderly rats, which indicated that senegenin may be a promising agent for the treatment of POCD. Source

Jiang T.,University of Houston | Ince N.F.,University of Houston | Jiang T.,Tian Tan Hospital | Wang T.,Haidian Hospital | And 4 more authors.
International IEEE/EMBS Conference on Neural Engineering, NER | Year: 2015

High density electrocorticogram (ECoG) of motor cortex was recorded during awake surgeries of two subjects with respectively epilepsy and brain tumor. Subjects were asked to execute spontaneous hand movements: extension and flexion during the experiments. We investigated the ECoG in time frequency plane during these hand movements and computed event-related desynchronization (ERD) and event-related synchronization (ERS) levels of beta (8-32 Hz) and gamma frequency bands. The distribution of ERD and ERS over channels provided an idea about the spatial distribution of the cortical activity within each task. In both subjects, consistently, the scale of power changes in beta and gamma band was larger in hand flexion compared to hand extension. In addition, the spatial distribution of ERD in beta and ERS in gamma band during flexion was larger than extension but highly overlapped between these tasks. Gamma band was more spatially localized than beta band activity and cortical patterns were more distinct between the tasks. Our preliminary results indicate that the high density ECoG can be effectively used in awake surgery for functional mapping. © 2015 IEEE. Source

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