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Zheng D.-D.,Nantong University | Ji S.-N.,Haian Hospital of Traditional Chinese Medicine | Chen C.,Nantong University | Deng X.-T.,Xinghua Peoples Hospital | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

There is an accumulating body of evidence indicating strong association between inflammation and the pathogenesis of atrial fibrillation (AF). IL-10 is a multifunctional anti-inflammatory cytokine that down-regulates cell-mediated immune responses and cytotoxic inflammatory responses. The aim of the present study is to investigate the association of IL-10 gene -592A/C polymorphism with AF in Han Chinese. 117 AF patients and 100 healthy volunteers were eligible for this study. The PCR-based restriction fragment length polymorphism (PCR-RFLP) technique was used to assess the genotypes frequencies. The distribution of the IL-10 -592A/C genotypes (AA, AC, and CC) was 55.00%, 35.00%, and 10.00% in the controls, and 71.79%, 23.08%, and 5.13% in AF subjects, respectively (p = 0.0335). The frequency of the A allele in the AF group was significantly higher than that in the control group (83.33% vs 72.50%, p = 0.0063). Compared with the CC genotype, the AA genotype had increased risk of AF in both unadjusted and adjusted analyses. The average serum IL-10 levels in AA genotype were statistically lower than in AC + CC genotype (p = 0.0000). These findings suggest that IL-10 -592A/C polymorphism is associated with AF and the A allele has increased risk for AF in Han Chinese. © 2014 E-Century Publishing Corporation. All rights reserved.

Hua L.,Haian Hospital of Traditional Chinese Medicine | Fan L.,Nantong University | Aichun W.,Haian Hospital of Traditional Chinese Medicine | Yongjin Z.,Haian Hospital of Traditional Chinese Medicine | And 2 more authors.
Tumor Biology | Year: 2014

Sineoculis homeobox homolog 1 (Six1) is one of the transcription factors that act as master regulators of development and is frequently dysregulated in cancers. However, the biological role of Six1 is not clear in osteosarcoma. To address the expression of Six1 in osteosarcoma cells, three osteosarcoma cell lines (U2OS, SaOS-2, and MG63) and a human osteoblastic cell line (hFOB1.19) were used to detect the expression of Six1 by quantitative real-time polymerase chain reaction and western blotting. The results showed that Six1 was upregulated in osteosarcoma cell lines compared to human osteoblastic cell line hFOB1.19. To investigate the role of Six1 in osteosarcoma cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry analysis, and transwell chamber assays were used to determine the effects of Six1 on the cell viability, cycle, apoptosis, and migration properties in U2OS cells. The results showed that Six1 could promote U2OS cell proliferation and migration, and suppress U2OS cell apoptosis. In addition, we investigated the effects of Six1 on the expression of following proteins (cyclin D1, caspase-3, and vascular endothelial growth factor-C (VEGF-C)). Results showed that Six1 could increase the expression of cyclin D1 and VEGF-C, and decrease the expression of caspase-3. All these data suggested that Six1 might be involved in the promotion of growth, proliferation, and migration of U2OS cells, as well as the inhibition of apoptosis of U2OS cells. These data might provide information for the prediction of osteosarcoma prognosis and potential targets for therapy of osteosarcoma. © 2013 International Society of Oncology and BioMarkers (ISOBM).

Wang Y.-J.,Haian Hospital of Traditional Chinese Medicine
World Chinese Journal of Digestology | Year: 2014

Aim: To assess the clinical efficacy of sertraline combined with otilonium bromide in irritable bowel syndrome (IBS) patients.Methods: Sixty IBS patients treated at our hospital from January 2010 to October 2012 were included. The patients were randomly divided into either a treatment group or a control group, with 30 patients in each group. The treatment group was orally administered with otilonium bromide (40 mg/time, 3 times/d) combined with sertraline (50 mg/time, 1 time/ d). The control group was given otilonium bromide (40 mg/time, 3 times/d) alone. The treatment lasted 4 wk in both group. Abdominal pain score, self-rating anxiety scale (SAS) score, self-rating depression scale (SDS) score and clinical efficacy before and 4 weeks after the therapy were compared between the two groups. Adverse reactions of drugs were also recorded.Results: The indexes showed no significant differences before treatment between the two groups (P >0.05). After 4 wk of the treatment, abdominal pain score (0.72 points ± 0.23 points vs 1.37 points ± 0.29 points), SAS score (36.57 points ± 5.44 points vs42.91 points ± 6.48 points), and SDS score (34.15 points ± 5.84 points vs 42.05 points ± 8.17 points) were all significantly lower in the treatment group than in the control group (P<0.05). The response rate was significantly higher in the treatment group than in the control group (86.67% vs60.00%, P<0.05). The incidence of adverse drug reactions was not statistically significant between the two groups (P>0.05).Conclusion: Compared to the otilonium bromide alone, sertraline combined with otilonium bromide is better in the remission of abdominal pain and negative emotions like mental anxiety and depression, and can improve the overall effectiveness. © 2014 Baishideng Publishing Group Inc. All rights reserved.

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