Blum S.,Kimron Veterinary Institute |
Krifucs O.,Kimron Veterinary Institute |
Goshen T.,Hachaklait |
Brenner J.,Kimron Veterinary Institute |
And 2 more authors.
Israel Journal of Veterinary Medicine | Year: 2012
The dynamics of anti-Porphyromonas levii antibody production was assessed by ELISA on a farm endemic for Bovine Necrotic Vulvovaginitis (BNNV) (farm A), one that underwent an outbreak a year previously (farm B) and one in which sporadic cases were observed in the past (farm C). Blood samples were taken 7, 4 and 1 week prior to the expected date of calving and on the first and fourth week after calving. Antibodies were present, pre-partum, in 44.4% and 34.8% of the animals on farm A and B, respectively. The antibody titre increased after calving in animals that developed BNVV by a factor of 3.3 and 2.8, on farms A and B respectively, but remained largely unchanged in animals that did not develop the disease. The frequency of development of BNVV in pre-partum seronegative and seropositive animals was similar: 42.5% and 38.3%, respectively. Antibody titres were significantly higher in animals that developed BNVV than in those that did not develop BNVV.
PubMed | German Research Center for Artificial Intelligence, HaChaklait and Kimron Veterinary Institute
Type: | Journal: Virus research | Year: 2016
In recent years there have been several major outbreaks of bovine ephemeral disease in the Middle East, including Israel. Such occurrences raise the need for quick identification of the viruses responsible for the outbreaks, in order to rapidly identify the entry of viruses that do not belong to the Middle-East BEFV lineage. This challenge was met by the development of a high-resolution melt (HRM) assay. The assay is based on the viral G gene sequence and generation of an algorithm that calculates and evaluates the GC content of various fragments. The algorithm was designed to scan 50- to 200-base-long segments in a sliding-window manner, compare and rank them using an Order of Technique of Preference by Similarity to Ideal Solution (TOPSIS) the technique for order preference by similarity to ideal solution technique, according to the differences in GC content of homologous fragments. Two fragments were selected, based on a match to the analysis criteria, in terms of size and GC content. These fragments were successfully used in the analysis to differentiate between different virus lineages, thus facilitating assignment of the viruses geographical origins. Moreover, the assay could be used for differentiating infected from vaccinated animales (DIVA). The new algorithm may therefore be useful for development of improved genotyping studies for other viruses and possibly other microorganisms.
Ezra E.,Israel Cattle Breeders Association |
Van Straten M.,Hachaklait |
Weller J.I.,Institute of Animal science
Animal | Year: 2016
A total of 1922 first generation crossbred cows born between 2005 and 2012 produced by inseminating purebred Israeli Holstein cows with Norwegian Red semen, and 7487 purebred Israeli Holstein cows of the same age in the same 50 herds were analyzed for production, calving traits, fertility, calving diseases, body condition score, abortion rate and survival under intensive commercial management conditions. Holstein cows were higher than crossbreds for 305-day milk, fat and protein production. Differences were 764, 1244, 1231 for kg milk; 23.4, 37.4, 35.6 for kg fat, and 16.7, 29.8, 29.8 for kg protein; for parities 1 through 3. Differences for fat concentration were not significant; while crossbred cows were higher for protein concentration by 0.06% to 0.08%. Differences for somatic cells counts were not significant. Milk production persistency was higher for Holstein cows by 5, 8.3 and 8% in parities 1 through 3. Crossbred cows were higher for conception status by 3.1, 3.6 and 4.7% in parities 1 through 3. Rates of metritis for Holsteins were higher than the crossbred cows by 7.8, 4.6 and 3.4% in parities 1 to 3. Differences for incidence of abortion, dystocia, ketosis and milk fever were not significant. Holstein cows were lower than crossbred cows for body condition score for all three parities, with differences of 0.2 to 0.4 units. Contrary to comparisons in other countries, herd-life was higher for Holsteins by 79 days. A total of 6321 Holstein cows born between 2007 and 2011 were higher than 765 progeny of crossbred cows backcrossed to Israeli Holsteins of the same ages for milk, fat and protein production. Differences were 279, 537, 542 kg milk; 10.5, 17.7, 17.0 kg fat and 6.2, 12.9, 13.2 kg protein for parities 1 through 3. Differences for fat concentration were not significant, while backcross cows were higher for protein percentage by 0.02% to 0.04%. The differences for somatic cell score, conception rate, and calving diseases other than metritis, were not significant. Holstein cows were lower than backcross cows by 1.5% to 2.5% for conception status in parities 1 to 3 and lower for body condition score for parities 1 and 2, with differences in the range of 0.06 to 0.09 units. Culling rates were higher, and herd-life lower for the crossbred cows. The gains obtained in secondary traits for crossbred cows did not compensate for the major reduction in production. © The Animal Consortium 2016
Shoshani E.,Extension Service |
Rozen S.,Extension Service |
Journal of Dairy Science | Year: 2014
We evaluated the effect of shortening the dry period (DP) on milk and energy-corrected milk (ECM) yields, milk components, colostrum quality, metabolic status, and reproductive parameters. Primiparous (n. = 372) and multiparous (n. = 400) Israeli Holstein cows from 5 commercial dairy herds were subjected to a 60-d or 40-d DP. Cows within each herd were paired according to milk production, age, days in milk, and expected calving. Analysis of the data from all cows, irrespective of age, revealed significant differences in milk and ECM yields that favored the 60-d DP, with a prominent effect in 2 of 5 examined herds. In primiparous cows, milk and ECM yields were similar between groups in 4 of 5 farms. In multiparous cows undergoing a 60-d (vs. 40-d) DP, milk and ECM yields were higher in 3 herds. These differences could not be explained by milk and ECM yields in cows diagnosed with metritis, ketosis, and mastitis (defined by a somatic cell count threshold of 250,000 cell/mL), distribution of infected and noninfected cows, or new infections during DP and after calving. Including the milk and ECM yields from an average of 19.55. d from the previous lactation revealed higher milk and ECM yields for 40-d (vs. 60-d) DP cows in all herds. Analyzing 2 consecutive lactations revealed similar milk and ECM yields between groups in 4 out of 5 herds. In 1 herd, yields were higher in the 40-d compared with the 60-d DP group. One week after calving, the nonesterified fatty acid concentrations of 40-d DP cows were significantly lower than those of 60-d DP cows, indicating better postpartum energy balance. Colostrum quality, measured as IgG concentration, did not differ between the 2 DP groups. Cows assigned to 40-d DP had better reproductive performance, as reflected by fewer days to first insemination, a lower proportion with >90. d to first insemination, and fewer days to pregnancy. With respect to primiparous cows, a short DP increased conception rate after first artificial insemination and decreased the proportion of nonpregnant cows after 150. d in milk. In light of these findings, we suggest that a short DP be applied for its economic and physiological benefits. This is highly relevant to dairy herds located in regions such as Israel, Spain, and Florida that suffer from reduced milk production during the hot season. © 2014 American Dairy Science Association.
Gild C.,HaChaklait |
Alpert N.,HaChaklait |
van Straten M.,HaChaklait
Israel Journal of Veterinary Medicine | Year: 2015
A large percentage of mature dairy cows experience some degree of hypocalcemia during the first days post-calving. In some cases calcium concentrations decline to levels that disrupt neuromuscular function, resulting in the clinical syndrome known as parturient paresis or milk fever. Post-parturient hypocalcemia is divided into clinical and subclinical forms. It has been established that cows suffering from clinical milk fever are susceptible to a variety of secondary conditions, however to the best of the authors’ knowledge there has been no evaluation of the impact of the subclinical form on production and reproductive parameters. The objective of this study was to investigate the association between subclinical hypocalcemia and postparturient disorders, production and reproductive parameters in Israeli dairy herds. Blood results for corrected calcium concentrations were analyzed from 634 mature cows from 5 farms. The subclinical hypocalcemic cows produced 3.2, 2.7 and 1.9 kg more milk in the first three milk recordings than the normocalcemic cows. Subclinical hypocalcmic cows did not show an increased risk for post-parturient diseases nor compromised reproduction parameters in comparison to normocalcemic cows. It was concluded that there was no negative impact of subclinical hypocalcemia on production and reproductive parameters in Israeli dairy cows after parturition. © 2015, Israel Veterinary Medical Association All rights reserved.