Cui Y.,Korea Advanced Institute of Science and Technology |
Kang M.-S.,National Institute of Biological Resources |
Woo S.-G.,Yonsei University |
Woo S.-G.,H Plus Eco Ltd |
And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013
A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2%), Brevibacterium salitolerans KCTC 19616T (96.7%), Brevibacterium album KCTC 19173T (96.2%) and Brevibacterium samyangense KCCM 42316T (96.2%). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15: 0, anteiso-C17: 0, C16: 0 and iso-C15: 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T (5KCTC 19800T5JCM 17458T). © 2013 IUMS.
Lee M.,H Plus Eco Ltd. |
Woo S.-G.,H Plus Eco Ltd. |
Woo S.-G.,Yonsei University |
Chae M.,H Plus Eco Ltd. |
Ten L.N.,Korea Institute of Energy Research
International Journal of Systematic and Evolutionary Microbiology | Year: 2010
A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07T, was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07T belongs to the family Alcaligenaceae, class Betaproteobacteria, and is related most closely to Pusillimonas ginsengisoli KCTC 22046T (98.6% sequence similarity) and Pusillimonas noertemannii BN9T (96.9%). The levels of 16S rRNA gene sequence similarity between strain MJ07T and members of all other recognized species of the family Alcaligenaceae were below 95.2%. The G+C content of the genomic DNA of strain MJ07T was 59.4 mol%. The detection of a quinone system with ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown aminolipids as major polar lipids and a fatty acid profile with C16 : 0 (32.0%), C17 : 0 cyclo (24.7%) and C19 : 0 cyclo ω8c (11.5%) as the major components supported the affiliation of strain MJ07T to the genus Pusillimonas. Strain MJ07T exhibited relatively low levels of DNA-DNA relatedness with respect to P. ginsengisoli KCTC 22046T (50±8%) and P. noertemannii KACC 13183T (18±7%). On the basis of its phenotypic and genotypic properties together with its phylogenetic distinctiveness, strain MJ07T (=KCTC 22455T =JCM 16386T) should be classified in the genus Pusillimonas as the type strain of a novel species, for which the name Pusillimonas soli sp. nov. is proposed. © 2010 IUMS.