Kenezy Gyula County Hospital

Debrecen, Hungary

Kenezy Gyula County Hospital

Debrecen, Hungary
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Torok P.,Debrecen University | Lorincz J.,Kenezy Gyula County Hospital | Dobrai M.,Kenezy Gyula County Hospital | Poka R.,Debrecen University
Middle East Fertility Society Journal | Year: 2017

Introduction: Tubal flushing effect is a published phenomena of hysterosalpingography in connection with tubal patency test. The aim of the study was to evaluate the flushing effect of the outpatient method: selective chromopertubation via office hysteroscopy (OHSC-SPT), and compare it to that of HSG. Materials and methods: Sixty infertile patients receiving OHSC-SPT (mean [SD] age: 34.1 [3.2] years) and 163 receiving HSG (32.7 [4.7] years) took part in the study. As part of the infertility work-up, diagnostic office hysteroscopy was performed to evaluate the uterine cavity. Patients with at least one patent tube had a 12-month follow-up, during which their obstetrical events, including successful conception of pregnancy after a sterile period, were recorded. The between-groups comparison was quantified by calculating a pregnancy proportion ratio. Results: Out of the 60 OHSC-SPT subjects, both tubes were blocked in 24 cases, and at least one of the tubes was patent in 36 cases. Out of these 36 cases, 7 (19.4%) spontaneous pregnancies were conceived; in three (8.3%) cases, pregnancy was conceived after intrauterine insemination (IUI) performed on male indication. The number of pregnancies observed in the HSG group (163 examined subjects) was 37 (32 spontaneous, 5 IUI-assisted) out of 153 followed-up subjects (24.4%). The pregnancy proportion ratio estimate was 1.15 (90% CI: 0.70-1.90; p = 0.671). Conclusions: The novel method of OHSC-SPT has a tubal flushing effect that is non-inferior to that observed with HSG. © 2017 Middle East Fertility Society.


Mihaly D.,Semmelweis University | Matula Z.,Hungarian Academy of Sciences | Changchien Y.-C.,Kenezy Gyula County Hospital | Papp G.,Semmelweis University | And 2 more authors.
Cancer Genetics | Year: 2017

The SS18-SSX chimeric gene is unique to synovial sarcoma. Multiple model systems including mouse cell lines expressing SS18-SSX, and genetically engineered mouse models of synovial sarcoma have been developed to elucidate the role of the chimeric gene in synovial sarcomagenesis. Although several cell lines stably expressing human SS18-SSX exist, there is an ongoing need for cell culture models enabling researchers to investigate the molecular mechanism of SS18-SSX action in a relevant cellular context. Here we report the establishment of a novel SS18-SSX1-expressing cell line created from immortalized human adipose tissue-derived mesenchymal stem cells via lentiviral transduction of the chimeric gene. Our cell line, termed SS-iASC, has been characterized by karyotyping and cell line identification, and stable expression of SS18-SSX1 has been verified using real-time PCR (RT-PCR), nested PCR, immunofluorescence, and immunoblotting. Focal cytokeratin positivity characteristic of synovial sarcoma but no β-Catenin, Bcl-2 or cyclin D1 expression was observed in SS-iASC. The novel cell line expressing SS18-SSX1 on a human adipose-derived stromal cell background is expected to be helpful in addressing the question whether the chimeric gene alone is sufficient to trigger the formation of synovial sarcoma. © 2017 Elsevier Inc.


PubMed | University of Szeged, University of Lisbon, Petz Aladar County Hospital, Kenezy Gyula County Hospital and 4 more.
Type: Journal Article | Journal: Chest | Year: 2016

Increased expression of the human epididymis protein 4 (HE4) was previously described in lung biopsy samples from patients with cystic fibrosis (CF). It remains unknown, however, whether serum HE4 concentrations are elevated in CF.Seventy-seven children with CF from six Hungarian CF centers and 57 adult patients with CF from a Czech center were enrolled. In addition, 94 individuals with non-CF lung diseases and 117 normal control subjects with no pulmonary disorders were analyzed. Serum HE4 levels were measured by using an immunoassay, and their expression was further investigated via the quantification of HE4 messenger RNA by using quantitative reverse transcription polymerase chain reaction in CF vsnon-CF respiratory epithelium biopsy specimens. The expression of the potential regulator miR-140-5p was analyzed by using an UPL-based quantitative reverse transcription polymerase chain reaction assay. HE4 was measured in the supernatants from unpolarized and polarized cystic fibrosis bronchial epithelial cells expressing wild-type or F508del-CFTR.Median serum HE4 levels were significantly elevated in children with CF (99.5 [73.1-128.9] pmol/L) compared with control subjects (36.3 [31.1-43.4] pmol/L; P< .0001). This observation was replicated in adults with CF (115.7 [77.8-148.7] pmol/L; P< .0001). In contrast, abnormal but lower HE4 concentrations were found in cases of severe bronchitis, asthma, pneumonia, and bronchiectasis. In patients with CF, the concentrations of HE4 were positively correlated with overall disease severity and C-reactive protein concentrations, whereas a significant inverse relationship was found between HE4 and the spirometric FEV1 value. Relative HE4 mRNA levels were significantly upregulated (P= .011) with a decreased miR-140-5p expression (P= .020) in the CF vsnon-CF airway biopsy specimens. Twofold higher HE4 concentrations were recorded in the supernatant of polarized F508del-CF transmembrane conductance regulator/bronchial epithelial cells compared with wild-type cells.HE4 serum levels positively correlate with the overall severity of CF and the degree of pulmonary dysfunction. HE4 may thus be used as a novel inflammatory biomarker and possibly also as a measure of treatment efficacy in CF lung disease.


Clemens B.,Kenezy Gyula County Hospital | Puskas S.,Debrecen University | Besenyei M.,Debrecen University | Kovacs N.,Kenezy Gyula County Hospital | And 7 more authors.
Epilepsy Research | Year: 2014

Aim: To investigate the effect of chronic VPA treatment of EEG functional connectivity in successfully treated idiopathic generalized epilepsy (IGE) patients. Patients and methods: 19-channel waking, resting-state EEG records of 26 IGE patients were analyzed before treatment (IGE) and after the 90th day of treatment (VPA), in seizure-free condition. Three minutes of artifact-free EEG background activity (without epileptiform potentials) was analyzed for each patient in both conditions. A group of 26 age-matched healthy normative control persons (NC) was analyzed in the same way. All the EEG samples were processed to LORETA (Low Resolution Electromagnetic Tomography) to localize multiple distributed sources of EEG activity. Current source density time series were generated for 33 regions of interest (ROI) in each hemisphere for four frequency bands. Pearson correlation coefficients (. R) were computed between all ROIs in each hemisphere, for four bands across the investigated samples. R values corresponded to intrahemispheric, cortico-cortical functional EEG connectivity (EEGfC). Group and condition differences were analyzed by statistical parametric network method. Main results: p<. 0.05, corrected for multiple comparisons: (1) The untreated IGE group showed increased EEGfC in the delta and theta bands, and decreased EEGfC in the alpha band (as compared to the NC group); (2) VPA treatment normalized EEGfC in the delta, theta and alpha bands; and (3) degree of normalization depended on frequency band and cortical region. Conclusions: VPA treatment normalizes EEGfC in IGE patients. © 2014 Elsevier B.V.


PubMed | University of Pécs, Balassa Janos Hospital, Bethesda Childrens Hospital, Kenezy Gyula County Hospital and Debrecen University
Type: Journal Article | Journal: Epilepsy research | Year: 2014

To investigate the effect of chronic VPA treatment of EEG functional connectivity in successfully treated idiopathic generalized epilepsy (IGE) patients.19-channel waking, resting-state EEG records of 26 IGE patients were analyzed before treatment (IGE) and after the 90th day of treatment (VPA), in seizure-free condition. Three minutes of artifact-free EEG background activity (without epileptiform potentials) was analyzed for each patient in both conditions. A group of 26 age-matched healthy normative control persons (NC) was analyzed in the same way. All the EEG samples were processed to LORETA (Low Resolution Electromagnetic Tomography) to localize multiple distributed sources of EEG activity. Current source density time series were generated for 33 regions of interest (ROI) in each hemisphere for four frequency bands. Pearson correlation coefficients (R) were computed between all ROIs in each hemisphere, for four bands across the investigated samples. R values corresponded to intrahemispheric, cortico-cortical functional EEG connectivity (EEGfC). Group and condition differences were analyzed by statistical parametric network method.p<0.05, corrected for multiple comparisons: (1) The untreated IGE group showed increased EEGfC in the delta and theta bands, and decreased EEGfC in the alpha band (as compared to the NC group); (2) VPA treatment normalized EEGfC in the delta, theta and alpha bands; and (3) degree of normalization depended on frequency band and cortical region.VPA treatment normalizes EEGfC in IGE patients.


PubMed | Preclinical Imaging and Biomarker Center, Kenezy Gyula County Hospital and Debrecen University
Type: | Journal: Journal of neuroimaging : official journal of the American Society of Neuroimaging | Year: 2016

The aim of this study was to reveal potential sources of systematic motion artifacts in stroke functional magnetic resonance imaging (fMRI) focusing on those causing stimulus-correlated motion on the individual-level and separate the motion effect on the fMRI signal changing from the activation-induced alteration at population level.Eleven ischemic stroke patients were examined by fMRI. The fMRI paradigm was based on passive ankle movement on both the healthy and the paretic legs side. Three individual-level motion correction strategies were compared and we introduced five measures to characterize each subjects in-scanner relative head movement. After analyzing the correlation of motion parameters and the subjects physiological scale scores, we selected a parameter to model the motion-related artifacts in the second-level analysis.At first (individual) level analysis, the noise-component correction-based CompCor method provided the highest -log10(p) value of cluster-level occurrence probability at 12.4/13.6 for healthy and paretic side stimulus, respectively, with a maximal z-value of 15/16.3. Including the motion parameter at second (group) level resulted in lower cluster occurrence values at 10.9/5.55 while retaining the maximal z-value.We proposed a postprocessing pipeline for ischemic stroke fMRI data that combine the CompCor correction at first level with the modeling of motion effect at second-level analysis by a parameter obtained from fMRI data. Our solution is applicable for any fMRI-based stroke rehabilitation study since it does not require any MRI-compatible motion capture system and is based on commonly used methods.


Chang Chien Y.-C.,Kenezy Gyula County Hospital | Karolyi K.,Kenezy Gyula County Hospital | Kovacs I.,Kenezy Gyula County Hospital
Annals of Clinical and Laboratory Science | Year: 2016

Low-grade fibromyxoid sarcoma (LGFMS) is a rare soft-tissue neoplasm with a deceptively benign histological appearance and low-grade malignant potential which is often mistaken for other reactive or benign lesions. It most frequently harbors balanced t(7;16) translocation, and leads to the fusion of the FUS and CREB3L2 genes which can be detected by cytogenetic methods. Young adults are most commonly affected and it typically arises in the deep proximal extremities or trunk with frequent recurrences. It may metastasize to the lungs several years later. Paravertebral LGFMS is exceedingly rare and only few cases have been published in the literature. In those cases the novel immunohistochemical markers and cytogenetic studies were not performed and morphological mimickers could not be confidently excluded. We present a rare case of paravertebral LGFMS from a 54-years-old male patient, which previously was misdiagnosed as a neurofibroma with subsequent tumor recurrence. The concrete diagnosis was established by using MUC4 immunohistochemical stain and fluorescent in situ hybridization (FISH), which showed diffuse membranous positivity and supernumerary ring chromosome with unbalanced FUS gene rearrangement, respectively. The latter finding is also rare and may cause diagnostic dilemma if one is not aware of such uncommon, but well-documented phenomenon. Differential diagnosis with other low-grade spindle cell tumors will also be discussed along with the literature review. © 2016 by the Association of Clinical Scientists, Inc.


Losonczy G.,Debrecen University | Fekete A.,Debrecen University | Voko Z.,Debrecen University | Takacs L.,Debrecen University | And 6 more authors.
Acta Ophthalmologica | Year: 2011

Purpose: Recent studies strongly support the role of genetic factors in the aetiology of age-related macular degeneration (AMD). We investigated the frequency of Tyr402His polymorphism of the complement factor H (CFH) gene, Ser69Ala polymorphism at LOC387715, rs11200638 polymorphism of the HTRA1 gene and different apolipoprotein E (ApoE) alleles in Hungarian patients with AMD in order to determine the disease risk conferred by these factors. Methods: In a case-control study, we performed clinical and molecular genetic examination of 105 AMD patients (48 patients in the early and 57 in the late subgroup) and 95 unrelated healthy controls. Detailed patient histories were recorded with the use of a questionnaire focusing on known risk factors for AMD. Results: In the early AMD subgroup, homozygous CFH, LOC387715 or HTRA1 polymorphisms conferred a 4.9-fold (95% confidence interval [CI] 1.7-14.2), 7.4-fold (95% CI 2.1-26.2) or 10.1-fold (95% CI 2.5-40.8) risk of disease, respectively. In the late AMD subgroup, carriers of two CFH, LOC387715 or HTRA1 risk alleles were at 10.7-fold (95% CI 3.7-31.0), 11.3-fold (95% CI 3.2-40.4) or 13.5-fold (95% CI 3.3-55.4) greater disease risk, respectively. Two CFH and one LOC387715 risk alleles in combination conferred a 15.0-fold (95% CI 3.2-71.0) increase in risk, whereas two LOC387715 risk alleles combined with one CFH risk allele was associated with a 14.0-fold (95% CI 2.1-95.1) increased risk for late AMD. ApoE alleles neither increased disease risk nor proved to be protective. Conclusions: The CFH, LOC387715 and HTRA1 polymorphisms are strongly associated with the development of AMD in the Hungarian population. The association is particularly pronounced when homozygous risk alleles are present and in the late stages of the disease. © 2009 The Authors. Journal compilation.


PubMed | Kenezy Gyula County Hospital
Type: Case Reports | Journal: Annals of clinical and laboratory science | Year: 2016

Low-grade fibromyxoid sarcoma (LGFMS) is a rare soft-tissue neoplasm with a deceptively benign histological appearance and low-grade malignant potential which is often mistaken for other reactive or benign lesions. It most frequently harbors balanced t(7;16) translocation, and leads to the fusion of the FUS and CREB3L2 genes which can be detected by cytogenetic methods. Young adults are most commonly affected and it typically arises in the deep proximal extremities or trunk with frequent recurrences. It may metastasize to the lungs several years later. Paravertebral LGFMS is exceedingly rare and only few cases have been published in the literature. In those cases the novel immunohistochemical markers and cytogenetic studies were not performed and morphological mimickers could not be confidently excluded.We present a rare case of paravertebral LGFMS from a 54-years-old male patient, which previously was misdiagnosed as a neurofibroma with subsequent tumor recurrence. The concrete diagnosis was established by using MUC4 immunohistochemical stain and fluorescent in situ hybridization (FISH), which showed diffuse membranous positivity and supernumerary ring chromosome with unbalanced FUS gene rearrangement, respectively. The latter finding is also rare and may cause diagnostic dilemma if one is not aware of such uncommon, but well-documented phenomenon. Differential diagnosis with other low-grade spindle cell tumors will also be discussed along with the literature review.


PubMed | Kenezy Gyula County Hospital
Type: | Journal: Case reports in pathology | Year: 2015

Massive localized lymphedema (MLL) is a rare pseudosarcomatous lesion due to localized lymphatic obstruction from variable causes. It is most common on medial aspect of thigh and inguinal region. Abdominal localization is rare and may cause clinical diagnostic confusion with other malignant tumors due to its large size. We report a case of abdominal wall MLL of a 56-year-old male patient under clinical suspicion of well differentiated liposarcoma. The literature search and differential diagnosis will be addressed. In doubt cases, immunohistochemical stain or fluorescent in situ hybridization can help to separate this entity from the other mimickers.

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