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Bang W.Y.,Gyeongnam National University of Science and Technology | Kim S.W.,Gyeongnam National University of Science and Technology | Kwon S.G.,Gyeongnam National University of Science and Technology | Hwang J.H.,Gyeongnam National University of Science and Technology | And 8 more authors.
Animal Genetics | Year: 2013

Using a methyl-DNA immunoprecipitation technique in combination with next-generation deep sequencing, we conducted comprehensive DNA methylation profiling of liver genomes from three pig breeds: Berkshire, Duroc and Landrace. The profiles revealed that the distribution patterns of methylation signals along the genome are conserved among the three pig breeds. Specifically, many signals in coding genes were found in introns, and most signals in the repetitive elements were identified in non-long terminal repeat (LTR) retrotransposons such as long and short interspersed repetitive elements, implying a significant association with alternative splicing and expression of retrotransposable elements respectively. Differentially methylated regions among the three pig breeds were identified in the non-LTR retrotransposons, suggesting that they may lead to differential retrotransposable element activity. Altogether, this study provides advanced swine methylome data and valuable resources for understanding the function of DNA methylation in the evolutionary divergence of different pig breeds. © 2013 The Authors, Animal Genetics © 2013 Stichting International Foundation for Animal Genetics. Source

Kwon S.G.,Gyeongnam National University of Science and Technology | Kwon S.G.,South Korean National Institute of Animal Science | Kwon S.G.,Gyeongsangnamdo Livestock Promotion Research Institute | Bang W.Y.,Gyeongnam National University of Science and Technology | And 35 more authors.
Indian Journal of Biochemistry and Biophysics | Year: 2013

The NADPH-dependent reduction activities of two paralogous pig AKR1C1s with and without 19 additional amino acid residues in C-terminus were evaluated against steroid hormones including 5α-dihydrotestosterone, testosterone, progesterone, androstenedione and 5α-androstane-3,17-dione, which act as substrates of the AKR1C1s. Among the hormones, the AKR1C1s exhibited the highest activity against 5a-dihydrotestosterone and the lowest activity against testosterone and progesterone. Furthermore, the AKR1C1s showed the largest differential activities against 5α-dihydrotestosterone, but no such change of activities was found against progestrone and testosterone. These results suggest that the C-terminal region of AKR1C1 plays an important effect in the reduction activities of pig AKR1C1. Thus, the differential activities of two AKR1C1 paralogs observed in the present study provide important insights in understanding the molecular evolution. Source

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