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Kwak H.-R.,National Academy of Agricultural Science | Kim J.-S.,Andong National University | Cho J.-D.,Andong National University | Lee J.-H.,Institute for Bioresources Research | And 3 more authors.
Plant Pathology Journal | Year: 2015

Melon necrotic spot virus (MNSV) was recently identi-fied on watermelon (Citrullus vulgaris) in Korea, dis-playing as large necrotic spots and vein necrosis on the leaves and stems. The average occurrence of MNSV on watermelon was found to be 30‒65% in Hapcheon and Andong City, respectively. Four isolates of the virus (MNSV-HW, MNSV-AW, MNSV-YW, and MNSV-SW) obtained from watermelon plants in different areas were non-pathogenic on ten general indicator plants, including Chenopodium quinoa, while they infected systemically six varieties of Cucurbitaceae. The virus particles purified by 10‒40% sucrose density gradient centrifugation had a typical ultraviolet spectrum, with a minimum at 245 nm and a maximum at 260 nm. The morphology of the virus was spherical with a diameter of 28‒30 nm. Virus particles were observed scattered throughout the cytoplasm of watermelon cells, but no crystals were detected. An ELISA was conducted using antiserum against MNSV-HW; the optimum concentrations of IgG and conjugated IgG for the assay were 1 μl/ml and a 1:8, 000‒1:10, 000 dilutions, respectively. Antiserum against MNSV-HW could capture specifically both MNSV-MN from melon and MNSV-HW from watermelon by IC/RT-PCR, and they were effectively detected with the same specific primer to produce product of 1, 172 bp. The dsRNA of MNSV-HW had the same profile (4.5, 1.8, and 1.6 kb) as that of MNSV-MN from melon. The nucleotide sequence of the coat protein of MNSV-HW gave a different phylogenetic tree, having 17.2% difference in nucleotide sequence compared with MNSV isolates from melon. © The Korean Society of Plant Pathology. Source

Ha G.-J.,Gyeongsang National University | Ha G.-J.,Gyeongsangnamdo Agricultural Research and Extension Service | Lee D.S.,Gyeongsang National University | Seung T.W.,Gyeongsang National University | And 6 more authors.
Korean Journal of Food Science and Technology | Year: 2015

The anti-amnesic effect of Artemisia argyi H against trimethyltin (TMT)-induced learning and memory impairment and its neuroprotective effect against H2O2-inducedoxidative stress were investigated. Cognitive behavior was examined by Y-maze and passive avoidance test for 4 weeks, which showed improved cognitive functions in mice treated with the extract. In vitro neuroprotective effects against H2O2-induced oxidative stress were examined using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide and lactate dehydrogenase (LDH) assays. A. argyi H. extract showed protective effects against H2O2-induced neurotoxicity; moreover, LDH release into the medium was inhibited. Finally, highperformance liquid chromatography (HPLC) analysis showed that eupatilin and jaceosidin were the major phenolic compounds in A. argyi H. extract. These results suggest that A. argyi H. could be a good source of functional substances to prevent neurodegenerative diseases. Source

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