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Lee S.Y.,South Korean National Institute of Animal Science | Lee J.L.,South Korean National Institute of Animal Science | Kim J.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Ko J.Y.,South Korean National Institute of Animal Science | And 4 more authors.
Horticulture Environment and Biotechnology | Year: 2013

This study was conducted to obtain intron-GUS gene-transferred plants by using somatic embryos (including embryogenic calluses) derived from 2 in vitro root explants of breeding lines (KR056002 and KR056006) bred by crossing between Rosa hybrida 'Tineke' and 'Mirinae Gold'. Calluses were induced from their root explants cultured in Schenk and Hildebrandt (SH) medium, which was supplemented with 5 or 11 mg·L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were generated from the calluses, which were cultured in SH medium supplemented with 3 mg·L-1 of 2,4-D. The ratio of callus formation from in vitro root explants was dependent on the concentration of 2,4-D supplement in the SH medium. Somatic embryos were generated from both KR056002 and KR056006, and embryogenesis was observed from calluses around the somatic embryo. The regenerative capacity of the embryo was maintained longer in calluses derived from in vitro root explants cultured on SH medium supplemented with 11 mg·L-1 of 2,4-D than with 5 mg·L-1 of 2,4-D. Six pseudo-intron-GUS transgenic lines were obtained. The expression rate of the intron-GUS gene in multi-shoots was 100%. After the formation of healthy roots, 3 transgenic lines were transferred to the greenhouse. All 3 lines were reconfirmed as intron-GUS gene transgenic plants by PCR and Southern analyses. © 2013 Korean Society for Horticultural Science. Source

Sa K.J.,Kangwon National University | Ko W.R.,Kangwon National University | Kim O.G.,Kangwon National University | Ma S.J.,Kangwon National University | And 2 more authors.
Genes and Genomics | Year: 2016

This study assessed the genetic and phenotypic variation of 90 super sweet corn inbred lines and performed association analyses of six agronomical traits using 100 simple sequence repeats (SSR), ultimately detecting 590 alleles, with an average of 5.90 alleles per locus. The average genetic diversity and Polymorphism information content values were 0.54 and 0.50, respectively. Using population structure analysis, inbred lines were divided into three major groups and one admixed group. Association analysis was performed with a general linear model using a Q-matrix (Q GLM) and a mixed linear model using Q and K-matrices (Q + K MLM). Q GLM found 33 marker-trait associations involving 20 SSR markers that were associated with six agronomic traits. Q + K MLM identified four marker-trait associations involving three markers that were associated with traits of days of tasseling (DT) and days of silking (DS). Q GLM and Q + K MLM detected four significant marker-trait associations (SMTAs), with a level of significance of P < 0.01. In overlapping SMTAs, phi051 was associated with DT, umc1708 was associated with DS, and umc2341 was associated with two traits: DT and DS. The detection of loci associated with traits in this study may provide greater opportunities to improve quality by marker-assisted selection (MAS). Finally, these results will be helpful for breeders in choosing parental lines for crossing combinations as well as markers for using MAS in super sweet corn breeding programs in Korea. © 2016 The Genetics Society of Korea and Springer-Science and Media Source

Lim S.H.,Catholic University of Daegu | Han H.S.,Kyungpook National University | Park J.H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Lee J.,Catholic University of Daegu
Journal of the Korean Society for Applied Biological Chemistry | Year: 2011

Methanol extract and its ethyl acetate fraction of peony root (Peonia lactiflora) were found to attenuate heart and brain injury in a rat model of ischemia-reperfusion. Thus, intake of peony root already approved for food in Korea could be beneficial for preventing myocardial and cerebral infarction caused by occlusion of coronary and cerebral arteries, respectively. Source

Choe M.,Gyeongsangbuk Do Agricultural Research and Extension Services | Hong S.-J.,Kyungpook National University | Lim J.-H.,Kyungpook National University | Kwak Y.,Kyungpook National University | And 4 more authors.
Journal of Applied Biological Chemistry | Year: 2013

Soil microbes are important integral components of soil ecosystem which have significant and diverse role in organic matter decomposition, nitrogen cycling, and nitrogen fixation. In this study an effective denaturing gradient gel electrophoresis (DGGE) method was employed for paddy soil microbial diversity survey. For optimum paddy soil microbial DNA extraction, different methods such as Lysis buffer, skim milk bead, sodium phosphate buffer, Epicentre Soil Master DNA extraction kit (Epicentre, USA) and Mo Bio Power Soil DNA kit (MO BIO, USA) methods were utilized. Among all the method, using Mo Bio Power Soil kit was most effective. DGGE analysis of Bacteria was carried out at 6% polyacylamide gel and 45-60% denaturing gradient in the optimal conditions. Whereas DGGE analysis of fungi was done at 6% polyacrylamide gel and 45-80% denaturing gradient in the optimal conditions. By applying the above assay, it was found that variation within the microbial community of paddy soil occurs by a factor of time. DGGE assay used in this study through for a variety of soil microbial analysis suggests the potential use of this method. © The Korean Society for Applied Biological Chemistry 2013. Source

Dawood T.,Radboud University Nijmegen | Yang X.,Radboud University Nijmegen | Visser E.J.W.,Radboud University Nijmegen | Te Beek T.A.H.,Netherlands Bioinformatics Center | And 10 more authors.
Plant Physiology | Year: 2016

Soil flooding is a common stress factor affecting plants. To sustain root function in the hypoxic environment, flooding-tolerant plants may form new, aerenchymatous adventitious roots (ARs), originating from preformed, dormant primordia on the stem. We investigated the signaling pathway behind AR primordium reactivation in the dicot species Solanum dulcamara. Transcriptome analysis indicated that flooding imposes a state of quiescence on the stem tissue, while increasing cellular activity in the AR primordia. Flooding led to ethylene accumulation in the lower stem region and subsequently to a drop in abscisic acid (ABA) level in both stem and AR primordia tissue. Whereas ABA treatment prevented activation of AR primordia by flooding, inhibition of ABA synthesis was sufficient to activate them in absence of flooding. Together, this reveals that there is a highly tissue-specific response to reduced ABA levels. The central role for ABA in the response differentiates the pathway identified here from the AR emergence pathway known from rice (Oryza sativa). Flooding and ethylene treatment also induced expression of the polar auxin transporter PIN2, and silencing of this gene or chemical inhibition of auxin transport inhibited primordium activation, even though ABA levels were reduced. Auxin treatment, however, was not sufficient for AR emergence, indicating that the auxin pathway acts in parallel with the requirement for ABA reduction. In conclusion, adaptation of S. dulcamara to wet habitats involved co-option of a hormonal signaling cascade well known to regulate shoot growth responses, to direct a root developmental program upon soil flooding. © 2016 American Society of Plant Biologists. All rights reserved. Source

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