Gyeongsangbuk do Agricultural Research and Extension Services

South Korea

Gyeongsangbuk do Agricultural Research and Extension Services

South Korea
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Kim S.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Park S.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Kim M.-K.,Gyeongsangbuk Do Agricultural Research and Extension Services | Lee S.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services
Scientific Papers of the Research Institute for Fruit Growing | Year: 2016

In the main producing province of jujube Zizyphus jujuba, Gyeongsnagbukdo, disorder of jujube flowers was found. The symptoms were browning of petal and dropping of flowers without fruit setting. The damaged flowers were collected and investigated, and confirmed that disorder of flower was caused on infection by Colletotrichum gloeosporioides. C. Gloeosporioides was isolated in 95.4% of discolored flower collected in Gyeongsan area, Aerial mycelium was white in early growth stage but turn to grayish with age. Orange color spore masses were produced outward from the center of the culture. The conidia were cylindrical with both apices rounded or with one apex rounded and the other end pointed. The conidial sizes were 9.2-13.2 x 3.2-5.8 μm. Apressoria were dark grey, ovate to clavate, and size was 5.9-7.2x4.2-5.1 μm. Bark and fruiting twig were surveyed to know overwintering place of C. gloeosporioides and the results are following that C. gloeosporioides was abundantly isolated from fruiting twigs, especially including fruiting twig appeared previous year, but not isolated from bark. This means that the reservoir of infection of anthracnose was considered as fruiting twig appeared previous year.


Lee S.-Y.,Kyungpook National University | Lim Y.-S.,Gyeongsangbuk do Agricultural Research and Extension Services | Jung H.-Y.,Kyungpook National University
Mycobiology | Year: 2016

In this study, the phylogeny and morphology of Mycosphaerella nawae (Dothideomycetes, Ascomycota) were examined using Korean and Japanese isolates, to establish the phylogenetic relationship between M. nawae and its allied species. Korean and Japanese isolates of M. nawae were collected from circular leaf spot-diseased leaves and were confirmed based on internal transcribed spacer (ITS) sequence data. Phylogenetic analysis was conducted using multiple genes, including the ITS region, 28S rDNA, ß-tubulin, translation elongation factor-1a, and actin genes. Our results revealed that M. nawae is closely related to members of the genus Phaeophleospora but are distant from the Ramularia spp. In addition, microscopic analysis revealed pseudothecia on the adaxial and abaxial surface of overwintered diseased leaves (ODL) and only on the abaxial surface of diseased leaves. Ascospores are oval to fusiform, one-septate, tapered at both ends, 1.7~3.1 × 8.1~14.1 μm, and were observed in ODL. Conidia are oval, guttulate, one-septate, 3.5~4.9 × 12.8~19.8 μm, and barely discernable on 30-day cultures. To our knowledge, this is the first report on the phylogeny of M. nawae, which is closely related to the genus Phaeophleospora, especially P. scytalidii. © The Korean Society of Mycology.


Lee S.-Y.,Kyungpook National University | Lim Y.-S.,Gyeongsangbuk do Agricultural Research and Extension Services | Jung H.-Y.,Kyungpook National University | Ohga S.,Kyushu University
Journal of the Faculty of Agriculture, Kyushu University | Year: 2017

In this study, we developed a nested PCR detection method based on draft genomic sequences of Mycosphaerella nawae and then applied the method to survey primary symptoms of circular leaf spot (CLS) and the seasonal ascospore scattering period. To develop the assay for detecting the fungus, 11 primers for the initial PCR and 19 primers for the nested PCR were designed (based on the glycoside hydrolase family 3 gene) using draft genomic sequences of M. nawae. Consequently, the primer pairs Mn-Fl/ Mn-Rl and Mn-nes-168f/Mn-nes-483r were selected and their specificities examined using regional M. nawae isolates, various healthy persimmon leaves, other fungal pathogens of persimmon, and allied species of M. nawae. Furthermore, the developed nested PCR method was applied for the diagnosis of the initial stage of CLS using both diseased and asymptomatic leaves. Additionally, scattered M. naivae ascospores collected by a spore sampler were surveyed from May to November 2015. Therefore, it is assumed that the nested PCR method can suggest the proper fungicide spraying period for CLS through anticipation of the ascospore quantity, and the early diagnosis of CLS will enable control of its development on persimmon.


Khan A.,Kyungpook National University | Khan A.,Kohat University of Science and Technology | Hamayun M.,Abdul Wali Khan University Mardan | Kang S.-M.,Kyungpook National University | And 4 more authors.
BMC Microbiology | Year: 2012

Background: Endophytic fungi are little known for exogenous secretion of phytohormones and mitigation of salinity stress, which is a major limiting factor for agriculture production worldwide. Current study was designed to isolate phytohormone producing endophytic fungus from the roots of cucumber plant and identify its role in plant growth and stress tolerance under saline conditions. Results: We isolated nine endophytic fungi from the roots of cucumber plant and screened their culture filtrates (CF) on gibberellins (GAs) deficient mutant rice cultivar Waito-C and normal GAs biosynthesis rice cultivar Dongjin-byeo. The CF of a fungal isolate CSH-6H significantly increased the growth of Waito-C and Dongjin-byeo seedlings as compared to control. Analysis of the CF showed presence of GAs (GA 1, GA 3, GA 4, GA 8, GA 9, GA 12, GA20 Van and GA 24) and indole acetic acid. The endophyte CSH-6H was identified as a strain of Paecilomyces formosus LHL10 on the basis of phylogenetic analysis of ITS sequence similarity. Under salinity stress, P. formosus inoculation significantly enhanced cucumber shoot length and allied growth characteristics as compared to non-inoculated control plants. The hypha of P. formosus was also observed in the cortical and pericycle regions of the host-plant roots and was successfully re-isolated using PCR techniques. P. formosus association counteracted the adverse effects of salinity by accumulating proline and antioxidants and maintaining plant water potential. Thus the electrolytic leakage and membrane damage to the cucumber plants was reduced in the association of endophyte. Reduced content of stress responsive abscisic acid suggest lesser stress convened to endophyte-associated plants. On contrary, elevated endogenous GAs (GA 3, GA 4, GA 12Van and GA 20) contents in endophyte-associated cucumber plants evidenced salinity stress modulation. Conclusion: The results reveal that mutualistic interactions of phytohormones secreting endophytic fungi can ameliorate host plant growth and alleviate adverse effects of salt stress. Such fungal strain could be used for further field trials to improve agricultural productivity under saline conditions. © 2012 Khan et al; licensee BioMed Central Ltd.


Sa K.J.,Kangwon National University | Ko W.R.,Kangwon National University | Kim O.G.,Kangwon National University | Ma S.J.,Kangwon National University | And 2 more authors.
Genes and Genomics | Year: 2016

This study assessed the genetic and phenotypic variation of 90 super sweet corn inbred lines and performed association analyses of six agronomical traits using 100 simple sequence repeats (SSR), ultimately detecting 590 alleles, with an average of 5.90 alleles per locus. The average genetic diversity and Polymorphism information content values were 0.54 and 0.50, respectively. Using population structure analysis, inbred lines were divided into three major groups and one admixed group. Association analysis was performed with a general linear model using a Q-matrix (Q GLM) and a mixed linear model using Q and K-matrices (Q + K MLM). Q GLM found 33 marker-trait associations involving 20 SSR markers that were associated with six agronomic traits. Q + K MLM identified four marker-trait associations involving three markers that were associated with traits of days of tasseling (DT) and days of silking (DS). Q GLM and Q + K MLM detected four significant marker-trait associations (SMTAs), with a level of significance of P < 0.01. In overlapping SMTAs, phi051 was associated with DT, umc1708 was associated with DS, and umc2341 was associated with two traits: DT and DS. The detection of loci associated with traits in this study may provide greater opportunities to improve quality by marker-assisted selection (MAS). Finally, these results will be helpful for breeders in choosing parental lines for crossing combinations as well as markers for using MAS in super sweet corn breeding programs in Korea. © 2016 The Genetics Society of Korea and Springer-Science and Media


Lee H.S.,Gyeongsangbuk do Agricultural Research and Extension Services | Park H.R.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim H.S.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim C.K.,Kyungpook National University
Journal of Plant Biotechnology | Year: 2015

This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum 'purple ND'. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: The basal of petal and TEOP: The end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA 1.0mgL-1 and BAP 2.0mgL-1 when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. © Korean Society for Plant Biotechnology.


Kim S.K.,Gyeongsangbuk do Agricultural Research and Extension Services | Choi H.J.,Gyeongsangbuk do Agricultural Research and Extension Services | Kang D.K.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim H.Y.,Keimyung University
Agronomy Research | Year: 2012

Proso millet (Panicum miliaceum L.) starches extracted from three varieties ('Andongjaerae', 'Danyangjaerae' and 'Andong 48ho') have been studied. Amylose content ranged between 1.2 and 21.5%. Starch content was from 84.4 to 85.67%. The onset temperature (To) of all starches ranged from 73.1 to 76.4 °C, peak temperature (Tp) ranged 78.0 to 81.5 °C, and their gelatinization enthalpy varied from -0.81 to -4.48 J g -1, respectively. The highest peak, highest breakdown, very high final viscosity, and very low setback were noted in 'Danyangjaerae'. X-ray diffraction angles showed in all starch granules possessing a typical A-type crystallinity, exhibiting strong deflections at about 15° 2θ, an unresolved double at 17°, 18° 2θ and separated angle 23° 2θ. The degree of crystallinity was higher in 'Andong 48ho' than in 'Andongjaerae' and 'Danyangjaerae'. A highly negative correlation was obtained between setback viscosity and relative crystallinity (r = 0.987, p < 0.01) with the amylose content and relative crystallinity (r = 0.964, p < 0.01). Positive correlation was observed between amylose content and setback viscosity (r = 0.869, p < 0.01).


Lim S.H.,Catholic University of Daegu | Han H.S.,Kyungpook National University | Park J.H.,Gyeongsangbuk do Agricultural Research and Extension Services | Lee J.,Catholic University of Daegu
Journal of the Korean Society for Applied Biological Chemistry | Year: 2011

Methanol extract and its ethyl acetate fraction of peony root (Peonia lactiflora) were found to attenuate heart and brain injury in a rat model of ischemia-reperfusion. Thus, intake of peony root already approved for food in Korea could be beneficial for preventing myocardial and cerebral infarction caused by occlusion of coronary and cerebral arteries, respectively.


Ko W.R.,Kangwon National University | Sa K.J.,Kangwon National University | Roy N.S.,Kangwon National University | Choi H.-J.,Gyeongsangbuk do Agricultural Research and Extension Services | Lee J.K.,Kangwon National University
Genetics and Molecular Research | Year: 2016

In this study, we compared the efficiency of simple sequence repeat (SSR) and sequence specific amplified polymorphism (SSAP) markers for analyzing genetic diversity, genetic relationships, and population structure of 87 super sweet corn inbred lines from different origins. SSR markers showed higher average gene diversity and Shannon’s information index than SSAP markers. To assess genetic relationships and characterize inbred lines using SSR and SSAP markers, genetic similarity (GS) matrices were constructed. The dendrogram using SSR marker data showed a complex pattern with nine clusters and a GS of 53.0%. For SSAP markers, three clusters were observed with a GS of 50.8%. Results of combined marker data showed six clusters with 53.5% GS. To analyze the genetic population structure of SSR and SSAP marker data, the 87 inbred lines were divided into groups I, II, and admixed based on the membership probability threshold of 0.8. Using combined marker data, the population structure was K = 3 and was divided into groups I, II, III, and admixed. This study represents a comparative analysis of SSR and SSAP marker data for the study of genetic diversity and genetic relationships in super sweet corn inbred lines. Our results would be useful for maize-breeding programs in Korea. © FUNPEC-RP.


PubMed | Gyeongsangbuk do Agricultural Research and Extension Services and Kangwon National University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

In this study, we compared the efficiency of simple sequence repeat (SSR) and sequence specific amplified polymorphism (SSAP) markers for analyzing genetic diversity, genetic relationships, and population structure of 87 super sweet corn inbred lines from different origins. SSR markers showed higher average gene diversity and Shannons information index than SSAP markers. To assess genetic relationships and characterize inbred lines using SSR and SSAP markers, genetic similarity (GS) matrices were constructed. The dendrogram using SSR marker data showed a complex pattern with nine clusters and a GS of 53.0%. For SSAP markers, three clusters were observed with a GS of 50.8%. Results of combined marker data showed six clusters with 53.5% GS. To analyze the genetic population structure of SSR and SSAP marker data, the 87 inbred lines were divided into groups I, II, and admixed based on the membership probability threshold of 0.8. Using combined marker data, the population structure was K = 3 and was divided into groups I, II, III, and admixed. This study represents a comparative analysis of SSR and SSAP marker data for the study of genetic diversity and genetic relationships in super sweet corn inbred lines. Our results would be useful for maize-breeding programs in Korea.

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