Gyeongsangbuk do Agricultural Research and Extension Services

South Korea

Gyeongsangbuk do Agricultural Research and Extension Services

South Korea
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Park S.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Kim S.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Woo J.-H.,Gyeongsangbuk Do Agricultural Research and Extension Services | Choi S.-Y.,Gyeongsangbuk Do Agricultural Research and Extension Services | Park S.-D.,Gyeongsangbuk Do Agricultural Research and Extension Services
Scientific Papers of the Research Institute for Fruit Growing | Year: 2015

This study was conducted to find environmentally friendly control methods and to investigate occurrence pattern of grapevine leaf spot, major disease in 'Campbell early' cultivar. Grapevine leaf spot caused by Pseudocercospora vitis is one of the most important disease in Korea. The occurrence pattern of leaf spot for 'Campbell early' cultivar was conducted in organic vineyards and conventional vineyards of Sangju, the main growing region of grape from 2012 to 2013. As results, the symptom of leaf spot was observed at last-June firstly in the organic vineyards and then the occurrence rate was gradually increased. The rate of lesion area was rapidly increased to 80.2% in last-August. To control grapevine leaf spot, some materials were tested and bicarbonate was selected. The optimum concentration of bicarbonates for control of grapevine leaf spot is 0.5%, and occurrence of grapevine leaf spot by treatment of 0.5% sodium bicarbonate and ammonium bicarbonate were 6.2 or 8.2%, respectively. This result showed that bicarbonate was more effective than Lime Bordeaux mixture in control of grapevine leaf spot widely used in grape organic cultivation for disease control. But phytotoxicity was observed in the treatment of 1% sodium bicarbonate and 2% potassium bicarbonate. So, it is expected that grapevine leaf spot will be successfully controlled by application of 0.5% bicarbonate.


Khan A.,Kyungpook National University | Khan A.,Kohat University of Science and Technology | Hamayun M.,Abdul Wali Khan University Mardan | Kang S.-M.,Kyungpook National University | And 4 more authors.
BMC Microbiology | Year: 2012

Background: Endophytic fungi are little known for exogenous secretion of phytohormones and mitigation of salinity stress, which is a major limiting factor for agriculture production worldwide. Current study was designed to isolate phytohormone producing endophytic fungus from the roots of cucumber plant and identify its role in plant growth and stress tolerance under saline conditions. Results: We isolated nine endophytic fungi from the roots of cucumber plant and screened their culture filtrates (CF) on gibberellins (GAs) deficient mutant rice cultivar Waito-C and normal GAs biosynthesis rice cultivar Dongjin-byeo. The CF of a fungal isolate CSH-6H significantly increased the growth of Waito-C and Dongjin-byeo seedlings as compared to control. Analysis of the CF showed presence of GAs (GA 1, GA 3, GA 4, GA 8, GA 9, GA 12, GA20 Van and GA 24) and indole acetic acid. The endophyte CSH-6H was identified as a strain of Paecilomyces formosus LHL10 on the basis of phylogenetic analysis of ITS sequence similarity. Under salinity stress, P. formosus inoculation significantly enhanced cucumber shoot length and allied growth characteristics as compared to non-inoculated control plants. The hypha of P. formosus was also observed in the cortical and pericycle regions of the host-plant roots and was successfully re-isolated using PCR techniques. P. formosus association counteracted the adverse effects of salinity by accumulating proline and antioxidants and maintaining plant water potential. Thus the electrolytic leakage and membrane damage to the cucumber plants was reduced in the association of endophyte. Reduced content of stress responsive abscisic acid suggest lesser stress convened to endophyte-associated plants. On contrary, elevated endogenous GAs (GA 3, GA 4, GA 12Van and GA 20) contents in endophyte-associated cucumber plants evidenced salinity stress modulation. Conclusion: The results reveal that mutualistic interactions of phytohormones secreting endophytic fungi can ameliorate host plant growth and alleviate adverse effects of salt stress. Such fungal strain could be used for further field trials to improve agricultural productivity under saline conditions. © 2012 Khan et al; licensee BioMed Central Ltd.


Sa K.J.,Kangwon National University | Ko W.R.,Kangwon National University | Kim O.G.,Kangwon National University | Ma S.J.,Kangwon National University | And 2 more authors.
Genes and Genomics | Year: 2016

This study assessed the genetic and phenotypic variation of 90 super sweet corn inbred lines and performed association analyses of six agronomical traits using 100 simple sequence repeats (SSR), ultimately detecting 590 alleles, with an average of 5.90 alleles per locus. The average genetic diversity and Polymorphism information content values were 0.54 and 0.50, respectively. Using population structure analysis, inbred lines were divided into three major groups and one admixed group. Association analysis was performed with a general linear model using a Q-matrix (Q GLM) and a mixed linear model using Q and K-matrices (Q + K MLM). Q GLM found 33 marker-trait associations involving 20 SSR markers that were associated with six agronomic traits. Q + K MLM identified four marker-trait associations involving three markers that were associated with traits of days of tasseling (DT) and days of silking (DS). Q GLM and Q + K MLM detected four significant marker-trait associations (SMTAs), with a level of significance of P < 0.01. In overlapping SMTAs, phi051 was associated with DT, umc1708 was associated with DS, and umc2341 was associated with two traits: DT and DS. The detection of loci associated with traits in this study may provide greater opportunities to improve quality by marker-assisted selection (MAS). Finally, these results will be helpful for breeders in choosing parental lines for crossing combinations as well as markers for using MAS in super sweet corn breeding programs in Korea. © 2016 The Genetics Society of Korea and Springer-Science and Media


Lee S.Y.,South Korean National Institute of Animal Science | Lee J.L.,South Korean National Institute of Animal Science | Kim J.-H.,Gyeongsangbuk do Agricultural Research and Extension Services | Ko J.Y.,South Korean National Institute of Animal Science | And 4 more authors.
Horticulture Environment and Biotechnology | Year: 2013

This study was conducted to obtain intron-GUS gene-transferred plants by using somatic embryos (including embryogenic calluses) derived from 2 in vitro root explants of breeding lines (KR056002 and KR056006) bred by crossing between Rosa hybrida 'Tineke' and 'Mirinae Gold'. Calluses were induced from their root explants cultured in Schenk and Hildebrandt (SH) medium, which was supplemented with 5 or 11 mg·L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were generated from the calluses, which were cultured in SH medium supplemented with 3 mg·L-1 of 2,4-D. The ratio of callus formation from in vitro root explants was dependent on the concentration of 2,4-D supplement in the SH medium. Somatic embryos were generated from both KR056002 and KR056006, and embryogenesis was observed from calluses around the somatic embryo. The regenerative capacity of the embryo was maintained longer in calluses derived from in vitro root explants cultured on SH medium supplemented with 11 mg·L-1 of 2,4-D than with 5 mg·L-1 of 2,4-D. Six pseudo-intron-GUS transgenic lines were obtained. The expression rate of the intron-GUS gene in multi-shoots was 100%. After the formation of healthy roots, 3 transgenic lines were transferred to the greenhouse. All 3 lines were reconfirmed as intron-GUS gene transgenic plants by PCR and Southern analyses. © 2013 Korean Society for Horticultural Science.


Lee H.S.,Gyeongsangbuk do Agricultural Research and Extension Services | Park H.R.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim H.S.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim C.K.,Kyungpook National University
Journal of Plant Biotechnology | Year: 2015

This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum 'purple ND'. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: The basal of petal and TEOP: The end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA 1.0mgL-1 and BAP 2.0mgL-1 when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. © Korean Society for Plant Biotechnology.


Kim S.K.,Gyeongsangbuk do Agricultural Research and Extension Services | Choi H.J.,Gyeongsangbuk do Agricultural Research and Extension Services | Kang D.K.,Gyeongsangbuk do Agricultural Research and Extension Services | Kim H.Y.,Keimyung University
Agronomy Research | Year: 2012

Proso millet (Panicum miliaceum L.) starches extracted from three varieties ('Andongjaerae', 'Danyangjaerae' and 'Andong 48ho') have been studied. Amylose content ranged between 1.2 and 21.5%. Starch content was from 84.4 to 85.67%. The onset temperature (To) of all starches ranged from 73.1 to 76.4 °C, peak temperature (Tp) ranged 78.0 to 81.5 °C, and their gelatinization enthalpy varied from -0.81 to -4.48 J g -1, respectively. The highest peak, highest breakdown, very high final viscosity, and very low setback were noted in 'Danyangjaerae'. X-ray diffraction angles showed in all starch granules possessing a typical A-type crystallinity, exhibiting strong deflections at about 15° 2θ, an unresolved double at 17°, 18° 2θ and separated angle 23° 2θ. The degree of crystallinity was higher in 'Andong 48ho' than in 'Andongjaerae' and 'Danyangjaerae'. A highly negative correlation was obtained between setback viscosity and relative crystallinity (r = 0.987, p < 0.01) with the amylose content and relative crystallinity (r = 0.964, p < 0.01). Positive correlation was observed between amylose content and setback viscosity (r = 0.869, p < 0.01).


Dawood T.,Radboud University Nijmegen | Yang X.,Radboud University Nijmegen | Visser E.J.W.,Radboud University Nijmegen | Te Beek T.A.H.,Netherlands Bioinformatics Center | And 10 more authors.
Plant Physiology | Year: 2016

Soil flooding is a common stress factor affecting plants. To sustain root function in the hypoxic environment, flooding-tolerant plants may form new, aerenchymatous adventitious roots (ARs), originating from preformed, dormant primordia on the stem. We investigated the signaling pathway behind AR primordium reactivation in the dicot species Solanum dulcamara. Transcriptome analysis indicated that flooding imposes a state of quiescence on the stem tissue, while increasing cellular activity in the AR primordia. Flooding led to ethylene accumulation in the lower stem region and subsequently to a drop in abscisic acid (ABA) level in both stem and AR primordia tissue. Whereas ABA treatment prevented activation of AR primordia by flooding, inhibition of ABA synthesis was sufficient to activate them in absence of flooding. Together, this reveals that there is a highly tissue-specific response to reduced ABA levels. The central role for ABA in the response differentiates the pathway identified here from the AR emergence pathway known from rice (Oryza sativa). Flooding and ethylene treatment also induced expression of the polar auxin transporter PIN2, and silencing of this gene or chemical inhibition of auxin transport inhibited primordium activation, even though ABA levels were reduced. Auxin treatment, however, was not sufficient for AR emergence, indicating that the auxin pathway acts in parallel with the requirement for ABA reduction. In conclusion, adaptation of S. dulcamara to wet habitats involved co-option of a hormonal signaling cascade well known to regulate shoot growth responses, to direct a root developmental program upon soil flooding. © 2016 American Society of Plant Biologists. All rights reserved.


Lim S.H.,Catholic University of Daegu | Han H.S.,Kyungpook National University | Park J.H.,Gyeongsangbuk do Agricultural Research and Extension Services | Lee J.,Catholic University of Daegu
Journal of the Korean Society for Applied Biological Chemistry | Year: 2011

Methanol extract and its ethyl acetate fraction of peony root (Peonia lactiflora) were found to attenuate heart and brain injury in a rat model of ischemia-reperfusion. Thus, intake of peony root already approved for food in Korea could be beneficial for preventing myocardial and cerebral infarction caused by occlusion of coronary and cerebral arteries, respectively.


Ko W.R.,Kangwon National University | Sa K.J.,Kangwon National University | Roy N.S.,Kangwon National University | Choi H.-J.,Gyeongsangbuk do Agricultural Research and Extension Services | Lee J.K.,Kangwon National University
Genetics and Molecular Research | Year: 2016

In this study, we compared the efficiency of simple sequence repeat (SSR) and sequence specific amplified polymorphism (SSAP) markers for analyzing genetic diversity, genetic relationships, and population structure of 87 super sweet corn inbred lines from different origins. SSR markers showed higher average gene diversity and Shannon’s information index than SSAP markers. To assess genetic relationships and characterize inbred lines using SSR and SSAP markers, genetic similarity (GS) matrices were constructed. The dendrogram using SSR marker data showed a complex pattern with nine clusters and a GS of 53.0%. For SSAP markers, three clusters were observed with a GS of 50.8%. Results of combined marker data showed six clusters with 53.5% GS. To analyze the genetic population structure of SSR and SSAP marker data, the 87 inbred lines were divided into groups I, II, and admixed based on the membership probability threshold of 0.8. Using combined marker data, the population structure was K = 3 and was divided into groups I, II, III, and admixed. This study represents a comparative analysis of SSR and SSAP marker data for the study of genetic diversity and genetic relationships in super sweet corn inbred lines. Our results would be useful for maize-breeding programs in Korea. © FUNPEC-RP.


PubMed | Gyeongsangbuk do Agricultural Research and Extension Services and Kangwon National University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

In this study, we compared the efficiency of simple sequence repeat (SSR) and sequence specific amplified polymorphism (SSAP) markers for analyzing genetic diversity, genetic relationships, and population structure of 87 super sweet corn inbred lines from different origins. SSR markers showed higher average gene diversity and Shannons information index than SSAP markers. To assess genetic relationships and characterize inbred lines using SSR and SSAP markers, genetic similarity (GS) matrices were constructed. The dendrogram using SSR marker data showed a complex pattern with nine clusters and a GS of 53.0%. For SSAP markers, three clusters were observed with a GS of 50.8%. Results of combined marker data showed six clusters with 53.5% GS. To analyze the genetic population structure of SSR and SSAP marker data, the 87 inbred lines were divided into groups I, II, and admixed based on the membership probability threshold of 0.8. Using combined marker data, the population structure was K = 3 and was divided into groups I, II, III, and admixed. This study represents a comparative analysis of SSR and SSAP marker data for the study of genetic diversity and genetic relationships in super sweet corn inbred lines. Our results would be useful for maize-breeding programs in Korea.

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