Gyeongbuk Veterinary Service Laboratory

Daegu, South Korea

Gyeongbuk Veterinary Service Laboratory

Daegu, South Korea
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Eo K.-Y.,Seoul Zoo | Kim Y.-H.,Gyeongbuk Veterinary Service Laboratory | Cho K.-H.,Gyeongsangbukdo Livestock Research Institute | Jang J.-S.,Kyungpook National University | And 3 more authors.
Pakistan Veterinary Journal | Year: 2011

Three Oriental Turtle-doves (Streptopelia orientalis) exhibiting lethargy, dyspnea, poor physical condition, and poor flight endurance, were rescued and referred to the Animal Health Center, Seoul Zoo, Korea. The doves had wart-like lesions on the legs and head. All of them died the following day after arrival, with the exception of one that survived for 6 days. Diphtheritic membranes on the tongue and oral mucosa were apparent at necropsy. Avian pox virus infection was suspected based on the proliferative skin lesions and oral diphtheritic lesions. Infection of the avian pox virus was confirmed by PCR using primers specific to the 4b core protein gene of avian pox virus. All cases were diagnosed with avian pox virus infection. This is believed to be the first description on natural infection of avian pox in Oriental Turtle-doves in Korea. © 2011 PVJ.


Gebru E.,Kyungpook National University | Damte D.,Kyungpook National University | Choi M.-J.,Kyungpook National University | Lee S.-J.,Kyungpook National University | And 2 more authors.
Veterinary Microbiology | Year: 2012

The antibacterial activity, selection of Escherichia coli (E. coli) mutants and mechanisms of fluoroquinolone resistance were investigated by integrating the minimum inhibitory concentration (MIC), mutant prevention concentration (MPC) and in vitro dynamic model approaches. Difloxacin and orbifloxacin, for which the above information has been scarce, were used. A range of area under curve over a 24h interval (AUC 24h)/MIC ratios and selected E. coli strains were investigated using the dynamic models. Continuous incubation for three days in the presence of difloxacin or orbifloxacin resulted in losses in E. coli susceptibility. An AUC 24h/MIC (AUC 24h/MPC)-dependent fluoroquinolone activity and selection of E. coli mutants was confirmed. Maximum losses in susceptibility occurred at AUC 24h/MIC ratios of 54 (orbifloxacin) and 57.3 (difloxacin). AUC 24h/MIC ratios of 169.8 (orbifloxacin) and 199.5 (difloxacin) were estimated to be protective against the selection of E. coli mutants, and the corresponding ratios based on AUC 24h/MPC predictions were 34 (orbifloxacin) and 36.3 (difloxacin). When integrating our in vitro data with pharmacokinetic data in dogs, the conventional clinical doses of both drugs were found to be inadequate to attain the above protective values for 90% of the mutant subpopulation (AUC 24h/MPC 90). Both target mutations, esp. at codon 83 (Ser to Leu) of gyrA, and overexpression of efflux pumps contributed to resistance development, with mutants also showing decreased susceptibility to enrofloxacin and marbofloxacin. Additional studies would determine the role of mutations found outside the QRDR, at codon 24 of gyrA, and at codon 116 of parC, and establish the significance of these observations in vivo. © 2011 Elsevier B.V.


Seo M.-G.,Animal and Plant Quarantine Agency | Ouh I.-O.,Animal and Plant Quarantine Agency | Kim M.,Pohang Center for Evaluation of Biomaterials | Lee J.,Animal and Plant Quarantine Agency | And 3 more authors.
Journal of Food Protection | Year: 2017

Tuberculosis, a chronic progressive disease, has been reported in bovine, swine, and primate species. Here, we report the first case of Mycobacterium tuberculosis infection in a Korean wild boar (Sus scrofa coreanus). The owners this domesticated boar brought it to the Gyeongbuk Veterinary Service Laboratory in Korea after it was found dead and severely emaciated. Demarcated yellowish white nodules were found around the larynx and retropharyngeal lymph node during necropsy. The lungs had diffuse fibrinous pleuritis, severe congestion, and scattered nodules. More nodules were found in the spleen. Tuberculosis is characterized by massive macrophage infiltration and central caseous necrosis; both characteristics were found in the lungs. Histopathologic examination revealed that the alveolar lumen had marked fibrosis and exudates. Examination of the fluid revealed extensive macrophage permeation. To confirm a Mycobacterium infection, PCR was performed using two primer sets specific to the rpoB gene of Mycobacterium; Mycobacterium was detected in the lungs and spleen. To identify the species of Mycobacterium, immunohistochemical evaluation was performed using antibodies against Mycobacterium tuberculosis and Mycobacterium bovis. The results revealed immunoreactivity against M. tuberculosis but not against M. bovis. The consumption of undercooked or raw meat from game animals may expose humans and other animals to sylvatic infection. Consequently, Koreans who ingest wild boar may be at risk of a tuberculosis infection. To reduce the risk of foodborne infection and maintain public health, continuous monitoring and control strategies are required.


Han S.M.,South Korean National Institute of Animal Science | Lee K.G.,South Korean National Institute of Animal Science | Yeo J.H.,South Korean National Institute of Animal Science | Oh B.Y.,Icheon si Agricultural Technology Service Center | And 6 more authors.
Poultry Science | Year: 2010

The effects of water supplementation of bee venom (BV) on performance, antioxidant activity, and liver function in Arbor Acres broiler chickens were investigated. Hence, 3 experimental treatment groups (control, 0.5 mg/L of BV, and 1 mg/L of BV) were allocated to 3 replicates of 5,000 one-day-old chicks each. The control group was kept on tap water, whereas the other 2 groups were supplied water supplemented with 0.5 and 1 mg of BV, respectively, per liter of drinking water. Broilers were provided ad libitum access to feed for the experimental period of 1 to 28 d of age. Supplementing drinking water with BV significantly increased BW gain at 28 d of age (P < 0.05). The average daily weight gain from d 1 to 28 was increased for birds supplemented with BV compared with control birds. The increase in BW gain was more pronounced with supplementation of 1 mg/L of BV compared with 0.5 mg/L of BV. An improved feed intake was noted in groups supplemented with BV as compared with control chicks. Liver function enzymes, aspartate aminotransferase, and alanine aminotransferase activities including total cholesterol, total protein, albumin, and globulin were not changed by BV supplementation. Tap water supplementation of BV did not alter the number of leukocytes, erythrocytes, heterophils, and lymphocytes. However, the antioxidative activities estimated as a superoxide dismutase-like activity of broiler chicks supplemented with BV was significantly increased (P < 0.05) in comparison with those without BV supplementation. These data indicate a possibility of better broiler performance through BV supplementation under conditions of severe stressful challenges the newly born chicks encounter. © 2010 Poultry Science.


Kim S.G.,Gyeongbuk Veterinary Service Laboratory | Kim Y.H.,Gyeongbuk Veterinary Service Laboratory | Chae M.J.,Kyungpook National University | Kim J.W.,National Veterinary Research and Quarantine Service | Lee Y.J.,Kyungpook National University
Journal of Animal and Veterinary Advances | Year: 2010

Brucellosis is one of the most important zoonoses which affects both animals and humans and leads to serious economic and public health problems. The aim of this study was to design, optimize and evaluate real-time PCR assay for Brucella sp. detection by targeting gap gene and to compare to those of conventional PCR assays. A low variation in CT values was observed for the gap gene target when the same quantity of DNA for 5 Brucella reference strains was used as template in the assays (CT: 21 -23 with 500 pg of Brucella DNA). No amplification products were observed in real-time PCR whatever the target with any of the 50 non-Brucella organisms tested. In the analytical sensitivity of real-time PCR assay based gap gene of B. abortus biovar 1 RB51, DNA concentration of 5 fg was successfully amplified and the sensitivity of the gap-based TaqMan real-time PCR assay was identical and 10-100 times higher than the sensitivity of the three conventional PCR. In the clinical trial, 9 (16.3%) and 11 (21.2%) among 52 blood samples from cows confirmed with B. abortus infection by Rose Bengal Spot agglutination test were positive in culture of B. abortus and gap real-time PCR, respectively. In conclusion, the use of the gap-based TaqMan real-time PCR assay appears promising due to it high sensitivity for the simple, faster and specific detection of the Brucella sp. © Medwell Journals, 2010.


Park S.,Yeungnam University | Shukla S.,Yeungnam University | Kim Y.,Gyeongbuk Veterinary Service Laboratory | Oh S.,Chonnam National University | And 2 more authors.
Microbiology and Immunology | Year: 2012

This study aimed to produce a polyclonal antibody against Cronobacter muytjensii (C. muytjensii, formerly called Enterobacter sakazakii) and to develop an immunoassay for its detection. The optimum production of rabbit anti-C. muytjensii immunoglobulin G (IgG) and chicken anti-C. muytjensii IgY was reached in weeks 8 and 9, respectively. Purification of rabbit anti-C. muytjensii IgG from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. As a result, sodium dodecyl sulfate-polyacrylamide gel electrophoresis produced two bands around 25 and 50 kDa, corresponding to a light and a heavy chain, respectively. The optimized conditions for sandwich enzyme-linked immunosorbent assay were using rabbit anti-C. muytjensii IgG (1 μg/mL) as a detection antibody and chicken anti-C. muytjensii IgY (10 μg/mL) as a capture antibody. In this assay, no cross-reactivity was observed with the other genera of pathogenic bacteria tested, which included Escherichia coli O157:H7, Salmonella typhimurium, Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes. The developed assay did not show cross-reactivity with other tested species of Cronobacter and Enterobacter genera such as C. turicensis, C. sakazakii, E. aerogenes, E. pulveris and E. helveticus. The detection limit of sandwich ELISA for C. muytjensii was found to be 2.0 × 104 colony forming units (CFU)/mL. In addition, detection of C. muytjensii in infant formula powder showed a low matrix effect on the detection curve of sandwich ELISA for C. muytjensii, the detection limit being found to be 6.3 × 104 CFU/mL. These findings demonstrate that the developed method is able to detect all strains of C. muytjensii. Hence, this ELISA technique has potent application for the rapid and accurate detection of C. muytjensii in dietary foods. © 2012 The Societies and Blackwell Publishing Asia Pty Ltd.


Oh J.,Kyungpook National University | Lee S.-H.,Kyungpook National University | Lee S.-J.,Kyungpook National University | Kim Y.-H.,Gyeongbuk Veterinary Service Laboratory | And 5 more authors.
Journal of Food Protection | Year: 2016

This study investigated the seroprevalence of Toxoplasma gondii in cattle in Gyeongbuk province, the largest producer of cattle in Korea. Of blood samples obtained from 568 animals, 0.5% (3 of 568) tested seropositive for T. gondii by using a commercial ELISA kit. Among young individuals (≤1 year old), adult individuals (2 years old), and old individuals (≥3 years old), 0 (0%) of 37, 3 (0.6%) of 474, and 0 (0%) of 57 were seropositive, respectively. Among male, female, and castrated individuals, 0 (0%) of 22, 0 (0%) of 74, and 3 (0.6%) of 472 were seropositive, respectively. Among individuals from east, south, and northwest regions of Gyeongbuk province, 0 (0%) of 155, 2 (0.7%) of 288, and 1 (0.8%) of 125 were seropositive, respectively. No statistical differences were observed among the groups. Although a low T. gondii seroprevalence was detected in cattle raised in Gyeongbuk province, toxoplasmosis is still a concern owing to the Korean habit of eating raw or undercooked meat and the consequent risk of Toxoplasma transmission to animals and humans.


PubMed | Gyeongbuk Veterinary Service Laboratory and Kyungpook National University
Type: Journal Article | Journal: Journal of food protection | Year: 2016

This study investigated the seroprevalence of Toxoplasma gondii in cattle in Gyeongbuk province, the largest producer of cattle in Korea. Of blood samples obtained from 568 animals, 0.5% (3 of 568) tested seropositive for T. gondii by using a commercial ELISA kit. Among young individuals (1 year old), adult individuals (2 years old), and old individuals (3 years old), 0 (0%) of 37, 3 (0.6%) of 474, and 0 (0%) of 57 were seropositive, respectively. Among male, female, and castrated individuals, 0 (0%) of 22, 0 (0%) of 74, and 3 (0.6%) of 472 were seropositive, respectively. Among individuals from east, south, and northwest regions of Gyeongbuk province, 0 (0%) of 155, 2 (0.7%) of 288, and 1 (0.8%) of 125 were seropositive, respectively. No statistical differences were observed among the groups. Although a low T. gondii seroprevalence was detected in cattle raised in Gyeongbuk province, toxoplasmosis is still a concern owing to the Korean habit of eating raw or undercooked meat and the consequent risk of Toxoplasma transmission to animals and humans.


Bang J.,Yeungnam University | Shukla S.,Yeungnam University | Kim Y.-H.,Gyeongbuk Veterinary Service Laboratory | Kim M.,Yeungnam University
Romanian Biotechnological Letters | Year: 2012

Due to increased rate of Salmonella infections in humans and animals, a rapid indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), was developed in this study for routine analytical detection of Salmonella typhimurium. Formalin killed whole cell lysate of S. typhimurium was subcutaneously injected as an antigen into rabbit to produce antibody. The optimum production of rabbit anti-S. typhimurium immunoglobulin G (IgG) was reached to the highest level on 8 week. A two step caprylic acid and ammonium sulfate precipitation method was used to purify rabbit anti-S. typhimurium IgG from the immunized rabbit sera. The purified IgG was used for the detection of S. typhimurium through IC-ELISA. The developed technique was able to detect 10 6 cells/ml of S. typhimurium. Also, the specificity of the proposed immunoassay was investigated with different foodborne pathogens including Escherichia coli O157:H7, Cronobacter muytjensii, Enterobacter aerogenes and Salmonella enteritidis which produced no significant reaction signal as compared with S. typhimurium. These findings indicate that IC-ELISA could be used as an alternative method for detection of S. typhimurium. © 2012 University of Bucharest.


PubMed | Gyeongbuk Veterinary Service Laboratory
Type: Journal Article | Journal: The Journal of veterinary medical science | Year: 2011

A pharmacokinetic study of a commercial florfenicol-tylosin (2:1) combination product was conducted in six beagle dogs after intravenous (IV) and intramuscular (IM) administration at doses of 10 mg/kg (florfenicol) and 5 mg/kg (tylosin). Serum drug concentrations were determined by a validated high performance liquid chromatography (HPLC) using UV detection. A rapid and nearly complete absorption of both drugs with a mean IM bioavailability of 103.9% (florfenicol) and 92.6% (tylosin), prolonged elimination half-life, and high tissue penetration with steady state volume of distribution of 2.63 l/kg (florfenicol) and 1.98 l/kg (tylosin) were observed. Additional studies, including pharmacodynamic and toxicological evaluation are required before recommendations can be made regarding the clinical application of the product in dogs.

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