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Lee I.-C.,Youngdong University | Bae J.-S.,Kyungpook National University | Kim T.,Andong National University | Kwon O.J.,Gyeongbuk Regional Innovation Agency | Kim T.H.,Daegu Haany University
Journal of the Korean Society for Applied Biological Chemistry

Phytochemical investigation on the polar constituents in the aerial parts of Thymus quinquecostatus var. japonica resulted in the isolation of three flavonoids along with four known phenolic components. The isolated compounds were characterized as 2(S)-5,7,3',5'-tetrahydroxyflavanone (1), (+)-taxifolin (2), (+)-aromadendrin (3), rosmarinic acid (4), caffeic acid (5), protocatechuic acid (6), and protocatechuic aldehyde (7) from comparisons of their physicochemical and spectroscopic data (1H-, 13C-NMR, two-dimentioal NMR, and mass spectrometery) with those of authentic samples and reference data. This is the first report on the isolation of compounds 1-7 from T. quinquecostatus var. japonica. In addition, compounds 2 and 4 exhibited the most potent pancreatic lipase inhibitory activities in a concentration-dependent manner with IC50 values of 15.4±0.8 and 62.8±2.7 μM, respectively. Source

Kim J.H.,Korea University | Pan J.H.,Korea University | Park H.G.,Korea University | Yoon H.G.,Yonsei University | And 4 more authors.
Journal of Agricultural and Food Chemistry

This study investigated the effects of dietary conjugated linoleic acid (CLA), in the form of free fatty acid (FFA-CLA) or triacylglycerol (TG-CLA), on serum and liver lipid composition and gene expression associated with lipogenesis and β-oxidation in high-fat-diet (HFD)-induced obese C57BL/6J mice. Animals were fed a control diet, HFD, HFD supplemented with 2% FFA-CLA, or HFD supplemented with 2% TG-CLA for 8 weeks. Supplementation with both forms of CLA significantly reduced the weights of whole body and adipose tissue and was positively associated with significant liver enlargement. Both forms of CLA significantly decreased serum TG concentration, but had no effect on total cholesterol levels, which were increased in mice fed HFD. There was a prominent increase in serum alanine aminotransferase (ALT) levels in mice that received either form of CLA. TG accumulation and lipogenic gene expression, including the expression of genes for fatty acid synthase (FAS), acetyl-coenzyme A carboxylase (ACC), and malic enzyme, were significantly lower in the livers of mice that received TG-CLA as compared to FFA-CLA. The gene expressions of sterol regulatory element binding protein-1c (SREBP-1c) in both liver and adipose tissue were suppressed in mice that were fed either form of CLA as compared to the mice fed HFD alone, whereas there were no increases in the levels of expression of β-oxidation-related genes. These findings demonstrated that free and esterified forms of CLA have differing effects on liver and adipose tissue lipogenesis. © 2010 American Chemical Society. Source

Choi U.-K.,Korea Advanced Institute of Science and Technology | Jeong Y.-S.,Kyungpook National University | Kwon O.,Gyeongbuk Regional Innovation Agency | Park J.-D.,Korea Food Research Institute | Kim Y.-C.,Korea Food Research Institute
International Journal of Molecular Sciences

This study was conducted to evaluate the effects of germinating soybeans under dark and light conditions on the quality characteristics of Korean soy sauce made with germinated soybeans. The germination rate of soybeans germinated under dark conditions (GSD) was higher than that of soybeans germinated under light conditions (GSL), whereas the lengths of sprouts and relative weights of GSL did not differ from those of GSD. The L, a, b, and δT values of GSL were significantly lower than GSD. The color of GSD remained yellow, while GSL changed to a green color due to photosynthesis by chlorophyll. The total amino acid contents in soy sauce fermented with soybeans germinated under dark conditions (SSGD) and soy sauce fermented with soybeans germinated under light conditions (SSGL) were lower than in soy sauce fermented with non-germinated soybeans (SNGS). The levels of isoflavone content in SSGD and SSGL were significantly increased compared to the SNGS. In conclusion, the germination of soybeans under dark and light conditions is not only an increasing organoleptic preference, but also has implications for the health benefits of Korean soy sauce. © 2011 by the authors; licensee MDPI, Basel, Switzerland. Source

Lee J.-Y.,Hoseo University | Bae H.-J.,Daegu Haany University | Park T.-S.,Korea Institute of Oriental Medicine | Kim T.-W.,Andong National University | And 7 more authors.
Journal of Applied Biological Chemistry

Biological activities such as anti-oxidative and anti-microbial of the Seungmakalgeuntang, a traditional prescription, were evaluated. The electron donating ability of water, ethanol, supercritical fluid and 1,3-butylene glycol extract of Seungmakalgeuntang showed more than 50% at a 100 ppm concentration. At a 1000 ppm concentration, the superoxide dismutase-like activities of ethanol and supercritical fluid extract of Seungmakalgeuntang showed less than 50%. xanthine oxidase inhibition effect of the supercritical fluid extract showed more than 70% at a 1,000 ppm concentration, which was higher than vitamin C. From the measurement on lipid oxidation, the Fe2+ chelating abilities of the supercritical fluid extract of Seungmakalgeuntang was more than 60% at a 100 ppm concentration. Also the Cu2+ chelating abilities of supercritical fluid extract Seungmkalgeuntangwas showed more than 60% at a 500 ppm concentration. Clear zones formed by sample against the human skin-resident microflora such as Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acne of ethanol and supercritical fluid extract of Seungmakalgeuntang showed the highest among all the extracts tested using a 4 mg/disc. The minimum inhibitory concentration (MIC) against both S. epidermidis and S. aureus showed 2,500 ppm in the extract of the supercritical fluid. Source

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