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Kim K.K.,Korea Research Institute of Bioscience and Biotechnology | Lee K.C.,Korea Research Institute of Bioscience and Biotechnology | Eom M.K.,Korea Research Institute of Bioscience and Biotechnology | Kim J.-S.,Gyeongbuk Institute for Marine Bioindustry | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

A novel bacterial strain designated GJW-30T was isolated from soil of the lava forest, Gotjawal, located in Aewol, Jeju, Korea. Strain GJW-30T was found to be strictly aerobic, Gram-negative and to form pleomorphic, non-motile rods and white colonies on R2A agar. The major fatty acids were identified as C18:1ω7c, C16:0 and C 17:0, the predominant isoprenoid quinone as Q-10, the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The cell-wall sugar pattern of strain GJW-30T was found to be composed of glucose, ribose and rhamnose and meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain GJW-30T is 62.2 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain GJW-30T forms a deep branch within the order Rhizobiales, sharing the highest level of sequence homology with Bradyrhizobium oligotrophicum LMG 10732T (93.6 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain GJW-30T is considered to represent a novel genus and species, for which the name Variibacter gotjawalensis gen. nov., sp. nov. (the type strain is GJW-30 T = KCTC 32391T = CECT 8514T = LMG 28093 T) is proposed. © 2014 Springer International Publishing.


Lee K.C.,Korea Research Institute of Bioscience and Biotechnology | Kim K.K.,Korea Research Institute of Bioscience and Biotechnology | Kim J.-S.,Gyeongbuk Institute for Marine Bioindustry | Kim D.-S.,World Heritage And Mt Hallasan Research Institute | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel bacterial strain, NKM-5T, was isolated from soil of a lava forest in Nokkome Oreum, Jeju, Republic of Korea. Cells of strain NKM-5T were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- and catalase-positive. Strain NKM-5T contained anteiso-C15:0 and iso-C16:0 as the major fatty acids; menaquinone-7 (MK-7) as the predominant isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, an unidentified phospholipid and three unidentified aminophospholipids as the polar lipids; and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 48.3mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain NKM-5T was most closely related to Cohnella lupini RLAHU4BT (96.9% sequence similarity) and fell into a clade in the genus Cohnella. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain NKM-5T represents a novel species of the genus Cohnella, for which the nameCohnella collisoli sp. nov. is proposed. The type strain is NKM-5T (=KCTC 33634T=CECT 8805T). © 2015 IUMS.


Kim M.U.,Gyeongbuk Institute for Marine Bioindustry | Cho Y.J.,Kyungpook National University | Lee S.Y.,Kangwon National University
Journal of Applied Biological Chemistry | Year: 2013

Cultured products (callus and exopolysaccharide) were obtained from suspension culture of Aloe vera callus, and the extracts of callus were further prepared with cold water or 60% ethanol solution. The ethanol extract of callus (AC) and exopolysaccharide (ACP) of 10 mg/mL exhibited the relatively higher suppression activity of 43.2-52.1% against hyaluronidase activity. Thus, their anti-inflammatory effects were further investigated using animal cell (Raw 264.7) in vitro. Though AC shows a slight suppression effect of cell survival rate (97%) using MTT assay in the presence of 400 μg/mL AC- dimethyl sulfoxide (DMSO), cell growth promotion was observed in the other samples of lower levels. It indicates that the ethanol extract of Aloe callus rarely affect cell survival rate in the ranges (200-400 μg/mL) used in the study. Using Griess reagent, the suppression of NO production by the aloe callus extract was analyzed by measuring the amount of the nitrite produced in Raw 264.7 culture activated by lipopolysaccharide (LPS). As a result, supplementation of AC-distilled water (DW) and AC-DMSO produced higher levels of NO than the positive control LPS. However, the NO suppression effect by ACP-DW was so intense that lower amount (80-100 μg/mL) suppressed NO production to the level of the control. The effect was attributed to the expression of the iNOS. Then, Raw 264.7 cells were stimulated with the LPS and expression of COX-2 protein level was analyzed depending on the Aloe suspension culture product treatment. The results showed that the ACP-DW supplemented medium did not express COX-2 by itself, and LPS stimulated COX-2 expression was slightly decreased. On the other hand, realtime-PCR analysis of the expression of inflammatory cytokine showed that IL-1β and TNF-α expression was highly suppressed in the ACP- distilled water supplemented medium. © The Korean Society for Applied Biological Chemistry 2013.


Lee K.C.,Korea Research Institute of Bioscience and Biotechnology | Kim K.K.,Korea Research Institute of Bioscience and Biotechnology | Kim J.-S.,Gyeongbuk Institute for Marine Bioindustry | Kim D.-S.,World Heritage And Mt Hallasan Research Institute | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

A novel bacterial strain, Back-11T, was isolated from sediment soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain Back-11T were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase-and catalase-positive. It contained anteiso-C15: 0 as the major fatty acid, menaquinone-7 (MK-7) as the predominant isoprenoid quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified aminophospholipids as the main polar lipids, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 45.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain Back-11T was most closely related to Paenibacillus taihuensis THMBG22T (95.5 % similarity) and fell into a clade in the genus Paenibacillus. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain Back-11T represents a novel species in the genus Paenibacillus, for which the name Paenibacillus baekrokdamisoli sp. nov. is proposed. The type strain is Back-11T (5KCTC 33723T=CECT 8890T). © 2016 IUMS.


Lee K.C.,Korea Research Institute of Bioscience and Biotechnology | Kim K.K.,Korea Research Institute of Bioscience and Biotechnology | Eom M.K.,Korea Research Institute of Bioscience and Biotechnology | Kim J.-S.,Gyeongbuk Institute for Marine Bioindustry | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel bacterial strain, designated SA3-7T, was isolated from soil of a lava forest located in Jeju, Republic of Korea. Cells of strain SA3-7T were Gram-stain-negative, oxidase-and catalasepositive, non-motile rods and produced creamy white colonies on ten-fold-diluted R2A agar. The isolate contained menaquinone-7 (MK-7) as the predominant isoprenoid quinone and summed feature 3 (C16:1ω7c/C16: 1ω6c), iso-C15: 0 and iso-C17: 0 3-OH as the major fatty acids. The DNA G+C content was 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain SA3-7T was related most closely to Mucilaginibacter frigoritolerans FT22T (96.7% sequence similarity) and that it formed a separate lineage in the genus Mucilaginibacter. Combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain SA3-7T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter gotjawali sp. nov. is proposed; the type strain is SA3-7T (=KCTC 32515T=CECT 8628T=DSM 29289T). © 2015 IUMS.

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