Gyeongbuk Institute for Marine Bio Industry

Uljin, South Korea

Gyeongbuk Institute for Marine Bio Industry

Uljin, South Korea

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Yayeh T.,Kyungpook National University | Yayeh T.,Debre Markos University | Im E.J.,Kyungpook National University | Kwon T.-H.,Gyeongbuk Institute for Marine Bio Industry | And 8 more authors.
International Immunopharmacology | Year: 2014

Eisenia bicyclis is edible brown algae recognized as a rich source of bioactive derivatives mainly phlorotannins reported for their anti-oxidant properties. Of all phlorotannins identified so far, dieckol has shown the most potent effect in anti-inflammatory, radical scavenging and neuroprotective functions. However, whether dieckol up-regulates hemeoxygenase 1 (HO-1) and this mediates its anti-inflammatory effect in murine macrophages remains poorly understood. Dieckol (12.5-50 μM) inhibited nitric oxide production and attenuated inducible nitric oxide synthase, phospho (p)-PI-3 K, p-Akt, p-IKK-α/β, p-IκB-α and nuclear p-NF-κBp65 protein expressions, and NF-κB transcriptional activity in LPS (0.1 μg/ml) stimulated murine macrophages. On the other hand, dieckol up-regulated HO-1which partly mediated its anti-inflammatory effect in murine macrophages. Thus, dieckol appeared to be a potential therapeutic agent against inflammation through HO-1 up-regulation. © 2014 Elsevier B.V.


PubMed | Health Science University, Daegu Haany University, Sungkyunkwan University, Kyungpook National University and 3 more.
Type: Journal Article | Journal: International immunopharmacology | Year: 2014

Eisenia bicyclis is edible brown algae recognized as a rich source of bioactive derivatives mainly phlorotannins reported for their anti-oxidant properties. Of all phlorotannins identified so far, dieckol has shown the most potent effect in anti-inflammatory, radical scavenging and neuroprotective functions. However, whether dieckol up-regulates hemeoxygenase 1 (HO-1) and this mediates its anti-inflammatory effect in murine macrophages remains poorly understood. Dieckol (12.5-50 M) inhibited nitric oxide production and attenuated inducible nitric oxide synthase, phospho (p)-PI-3K, p-Akt, p-IKK-/, p-IB- and nuclear p-NF-Bp65 protein expressions, and NF-B transcriptional activity in LPS (0.1 g/ml) stimulated murine macrophages. On the other hand, dieckol up-regulated HO-1 which partly mediated its anti-inflammatory effect in murine macrophages. Thus, dieckol appeared to be a potential therapeutic agent against inflammation through HO-1 up-regulation.


Kwon T.-H.,Gyeongbuk Institute for Marine Bio Industry | Kwon T.-H.,Andong National University | Kim T.-W.,Andong National University | Kim C.-G.,Gyeongbuk Institute for Marine Bio Industry | Park N.-H.,Gyeongbuk Institute for Marine Bio Industry
Journal of Food Science | Year: 2013

In this study, we aimed to elucidate the antioxidant capacity of Eisenia bicyclis and evaluated its antioxidant activity using various assay systems such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, reducing power ability, and content of total polyphenol. Among all the performed experiments, the ethyl acetate fraction of E. bicyclis exhibited higher antioxidant activities. From this finding, isolation and purification were performed on the ethyl acetate fraction and identified dieckol and phlorofucofureoeckol-A by spectroscopic analyses including FAB-mass in the negative mode, 1H NMR, 13C NMR, 1H-1H COSY, HMQC, and HMBC spectra. Interestingly, ABTS radical scavenging activities of dieckol and phlorofucofuroeckol showed strong effects of 65.36% and 70.38% at a concentration of 50 μg/mL, respectively. DPPH radical scavenging and reducing power abilities were increased in a dose-dependent manner at various concentrations. These results suggest that dieckol and phlorofucofuroeckol-A of E. bicyclis may play an important role in protection from oxidative stress involving reactive oxygen species and may contribute to the development of new bio products, for example, a useful preservative to improve food quality and a drug for various oxidative damage-associated diseases. Practical Application: The results suggest that dieckol and phlorofucofuroeckol-A can be utilized as a natural source for potential application of antioxidant in food industry and drug for oxidative damage-associated diseases. © 2013 Institute of Food Technologists®.


Woo J.-H.,Korea Ocean Research and Development Institute | Woo J.-H.,Gyeongbuk Institute for Marine Bio Industry | Kang J.-H.,Korea Ocean Research and Development Institute | Hwang Y.-O.,Korea Ocean Research and Development Institute | And 3 more authors.
Journal of Bioscience and Bioengineering | Year: 2010

As a continuous effort of developing highly enantioselective epoxide hydrolase from marine microorganisms, it was found that Rhodobacterales bacterium HTCC2654 was highly enantioselective toward racemic glycidyl phenyl ether (GPE). An open reading frame (ORF) encoding a putative epoxide hydrolase (EHase) was cloned from the genome of R. bacterium HTCC2654, followed by expression and purification in Escherichia coli. The purified EHase (REH) hydrolyzed (S)-GPE preferentially over (R)-GPE. Enantiopure (R)-GPE from kinetic resolution of 29.2 mM racemic GPE using the purified REH could be obtained with enantiopurity of more than 99.9% enantiomeric excess (ee) and 38.4% yield (theoretical, 50%) within 20 min (enantiomeric ratio (E-value): 38.4). The enantioselective activity of REH toward GPE was also confirmed by the analysis of the vicinal diol, 3-phenoxy-1,2-propanediol. To our knowledge, this study demonstrates the highest enantioselective resolution of racemic GPE using a purified biocatalyst among the known native EHases. © 2009 The Society for Biotechnology, Japan.


Kim S.J.,Gyeongbuk Institute for Marine Bio Industry | Chung Y.,Korea University | Lee T.-K.,Maritime and Ocean Engineering Research Institute
Ocean and Polar Research | Year: 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide (H 2O 2); strong correlation between H 2O 2 concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 μg/L) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.


Woo J.-H.,Korea Ocean Research and Development Institute | Woo J.-H.,Gyeongbuk Institute for Marine Bio Industry | Hwang Y.-O.,Korea Ocean Research and Development Institute | Kang J.-H.,Korea Ocean Research and Development Institute | And 3 more authors.
Journal of Bioscience and Bioengineering | Year: 2010

Previously we reported that an epoxide hydrolase (EHase) from Novosphingobium aromaticivorans could preferentially hydrolyze (R)-styrene oxide. In this study, we demonstrate that the purified NEH could be also effective in chiral resolution of racemic epichlorohydrin (ECH). Particularly, the purified NEH showed excellent hydrolyzing activity toward ECH to complete the reaction at a short period of incubation time. Enantiopure (S)-ECH could be obtained with a high enantiopurity of more than 99.99% enantiomeric excess (ee) and yield of 20.7% (theoretical, 50%). The chiral resolution of the purified NEH toward ECH was not susceptible to substrate inhibition by 500 mM racemic ECH. © 2010 The Society for Biotechnology, Japan.


Patent
Gyeongbuk Institute For Marine Bio Industry | Date: 2012-12-03

Disclosed is a novel compound having biosurfactant activity that is produced by Aureobasidium pullulans L-3-GPY strains (KCCM11200P). The novelty of the structure of the compound having biosurfactant activity was evaluated using spectroscopy, and it was determined that the biosurfactant produced by the strains has excellent surfactant activity. Active ingredients of the biosurfactant may be used for various purposes, for example, for producing a composition for washing and cleaning agents. Furthermore, they can be used in a variety of industrial fields where chemically synthesized surfactants are used such as medicines, foods, cosmetics, onshore and offshore oil decontamination, degradation of oil and fat in treatment tanks, and the like where chemically synthesized surfactants are used.


PubMed | Gyeongbuk Institute for Marine Bio Industry and Korea Advanced Institute of Science and Technology
Type: Journal Article | Journal: Asian Pacific journal of tropical medicine | Year: 2016

To determine skin whitening and wrinkle improvement efficacy, glycoprotein fractions were extracted from liquid extracts of boiled sea cucumber and their effects on tyrosine and elastase inhibitory activities were assayed.Fractions above and below 50kDa (>50kDa and <50kDa) were extracted via a series of steps involving: boiling, filtering, desalting and freeze drying. Cytotoxicity, skin whitening and wrinkle-removing effects of boiled liquid were determined.Our MTT data showed that neither glycoprotein fraction of boiled liquid induces cellular cytotoxicity up to a concentration of 10mg/mL treatment of the mouse melanoma cell line, B16F10, with 10mg/mL >50kDa enhanced tyrosinase and elastase inhibitory activities by 50.84% and 28.78%, respectively. Correlations of the >50kDa concentration with tyrosinase inhibitory (R2=0.968) and elastase inhibitory (R2=0.983) efficacy were significant.>50kDa glycoprotein fraction isolated from liquid extracts of boiled sea cucumber, which can serve as a functional cosmetic ingredient for whitening and wrinkle improvement of skin.


PubMed | Daegu Haany University, East Sea Research Institute, KIOST, Gyeongbuk Institute for Marine Bio Industry and Korea Institute of Oriental Medicine
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015

We evaluated the preventive effects of four types of seawater collected in Republic of Korea on hairless mice with 2,4-dinitrochlorobenzene- (DNCB-) induced allergic/atopic dermatitis (AD). The anti-inflammatory effects were evaluated by measuring tumor necrosis factor- (TNF-) and interleukins (ILs). Glutathione (GSH), malondialdehyde (MDA), superoxide anion, and inducible nitric oxide synthase (iNOS) were measured to evaluate the antioxidant effects. Caspase-3 and poly (ADP-ribose) polymerase (PARP) were observed to measure the antiapoptotic effects; matrix metalloproteinase- (MMP-) 9 levels were also evaluated. Mice with AD had markedly higher clinical skin severity scores and scratching behaviors; higher TNF- and ILs (1, 10, 4, 5, and 13) levels; higher MDA, superoxide anion, caspase-3, PARP, and MMP-9 levels; and greater iNOS activity. However, the severity of AD was significantly decreased by bathing in seawaters, but it did not influence the dermal collagen depositions and skin tissue antioxidant defense systems. These results suggest that bathing in all four seawaters has protective effects against DNCB-induced AD through their favorable systemic and local immunomodulatory effects, active cytoprotective antiapoptotic effects, inhibitory effects of MMP activity and anti-inflammatory and antioxidative effects.


PubMed | Kyungil University, Daegyeong Institute for Regional Program Evaluation, Gyeongbuk Institute for Marine Bio Industry and Andong National University
Type: Comparative Study | Journal: Journal of the science of food and agriculture | Year: 2015

Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown.We investigated the anti-obesity effects of 6,6-bieckol, 6,8-bieckol, 8,8-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor (PPAR), CCATT/enhancer-binding protein (C/EBP) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50g mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6-bieckol markedly decreased lipid accumulation and expression levels of PPAR, C/EBP, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA).These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis.

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