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Hyderabad, India

Rehan H.S.,Lady Hardinge Medical College | Chopra D.,Lady Hardinge Medical College | Sah R.K.,Lady Hardinge Medical College | Chawla T.,Lady Hardinge Medical College | And 2 more authors.
Journal of Infection and Public Health | Year: 2012

Background: Unsafe injection practices are prevalent worldwide and may result in spread of infection. Thus the present study was planned to observe the injection practices of healthcare professionals (HCP), including aseptic precautions and disposal of used syringes/needle. Materials and methods: Injection practices were observed in the outpatients and inpatients departments. Questionnaire was designed, tested and administered for this purpose. Results: 130 patients receiving injections were observed. Overall injection practices of the HCP were satisfactory. However, unsafe practices with respect to not washing hands (95.4%), not wearing/changing gloves (61.6%), recapping of needles (12.2%), wiping of needle with swab (15.4%) and breaking of ampoule with solid object (44.4%) were observed. Conclusion: The problem of unsafe injections can be successfully addressed by organizing continuing medical education/symposium/workshops for improving the knowledge, attitude and practices of the HCP. Periodic monitoring and such interventions may also further improve safe injection practices. © 2012 King Saud Bin Abdulaziz University for Health Sciences.

Ahmed S.,Aligarh Muslim University | Ahmed S.,King Saud University | Lin H.-C.,National Taiwan University | Nizam I.,Aligarh Muslim University | And 3 more authors.
Records of Natural Products | Year: 2014

(-)-Fisetinidol-(4α,8)-[(-)-fisetinidol-(4α,6) ]-(+)-catechin (1), a proanthocyanidin, was isolated from the bark of Acacia leucophloea. Its structure including absolute configuration was elucidated on the basis of spectroscopic analysis and chemical correlation. The 1H NMR spectrum of this compound, exhibiting exceptional complex signals attributable to rotational isomerism, and the reported data were obtained at elevated temperature in methyl ether acetate form. This work provided the 1H and 13C NMR assignments for 1 and its rotational isomer as the free phenolic form at ambient temperature for the first time. Compound 1 showed inhibitory activity against α-glucosidase type IV from Bacillus stearothermophilus with the IC50 value of 102.3 μM. © 2014 ACG Publications. All rights reserved.

Ramarao N.T.,Alkem Research Center | Ramarao N.T.,Krishna University | Vidyadhara S.,Chebrolu Hanumaiah Institute of Pharmaceutical science | Basaveswara Rao M.V.,Krishna University | And 2 more authors.
Journal of Analytical Chemistry | Year: 2015

A simple, rapid, specific and precise liquid chromatography–tandem mass spectrophotometric (LC-MS/MS) method was developed and validated for quantification of Erlotinib, in human plasma. Erlotinib d6 was used as internal standard, added to plasma sample prior to extraction using acetonitrile as a precipitating agent. Chromatographic separation was achieved on Phenomenex Luna C18 column (50 mm × 1 mm, 5 μm) with acteonitrile: 10 mM ammonium formate buffer (80: 20, v/v) as an isocratic mobile phase with a flow rate of 0.5 mL/min. Quantitation was performed by transition of 394.0 → 278.0 (m/z) for Erlotinib and 400.0 → 278.0 (m/z) for Erlotinib d6. The lower limit of quantitation was 10 ng/mL with a 100 mL plasma sample. The concentrations of nine working standards showed linearity between 10 and 5000 ng/mL (r2 ≥ 0.9992). Chromatographic separation was achieved within 3.50 min. The average extraction recoveries of 3 quality control concentrations were 94.25% for Erlotinib and 93.55% for Erlotinib d6. The coefficient of variation was ≤15% for intraand inter-batch assays. The developed method was successfully applied to the determination of Erlotinib pharmacokinetics after oral administration. © 2015, Pleiades Publishing, Ltd.

Harjai M.,University of Lucknow | Bogra J.,University of Lucknow | Gupta R.,University of Lucknow | Gurumoorthi R.,University of Lucknow | And 3 more authors.
Journal of Anesthesia and Clinical Research | Year: 2014

Background: Various drugs are used for premedication to reduce anxiety and to provide hemodynamic stability. The study was designed to investigate the optimum dose of oral clonidine administered preoperatively with regard to its anxiolytic efficacy and its effect on hemodynamics and sedation. We studied the effect of three different doses of oral clonidine on surgeries below umbilicus which were administered intrathecal bupivacaine. Methods: A placebo controlled double blind study was conducted on 120 patients scheduled for surgeries below umbilicus. Group 1 received oral placebo, group 2 received oral clonidine 3 μg kg-1, group 3 received oral clonidine 4 μg kg-1 and group 4 received oral clonidine 5 μg kg-1 along with 0.5% heavy bupivacaine 0.3 μg kg-1 intrathecally in each group. Outcomes assessed were anxiolysis through VAS, level of sensory block, time to reach highest sensory segment, regression to L1 segment, sedation score, bradycardia and hypotension. Results: There was improved block duration and sedation with the different doses of clonidine. Time for the sensory block to regress to L1 and rescue analgesia was longest in 4 followed by groups 3 and 2. There was significant dose dependent decrease in VAS anxiety score between group 1 and other clonidine groups in intraoperative and post-operative period. However, the episodes of bradycardia and hypotension were highest in 4 group. Conclusion: Preoperative oral clonidine 4 μg kg-1 appears to be the optimum dose for optimization of spinal anaesthesia with bupivacaine as it prolongs the sensory block maximally with minimal side effects. © 2014 Bogra J, et al.

Sen S.,GVK Biosciences | Potti V.R.,GVK Biosciences | Potti V.R.,Andhra University | Surakanti R.,GVK Biosciences | And 3 more authors.
Organic and Biomolecular Chemistry | Year: 2011

A highly efficient enantioselective SNAr reaction of chiral acyl bicyclic lactam with substituted-2,4-dinitrobenzenes was developed, affording products containing quarternary stereogenic centers. They are further utilized towards an enantioselective synthesis of spirooxoindoles. © 2011 The Royal Society of Chemistry.

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