Guys And St Thomas Center For Preimplantation

London, United Kingdom

Guys And St Thomas Center For Preimplantation

London, United Kingdom

Time filter

Source Type

Jacquet L.,King's College | Stephenson E.,King's College | Collins R.,Guys Hospital | Patel H.,Guys And St Thomas Center For Preimplantation | And 7 more authors.
EMBO Molecular Medicine | Year: 2013

Here, we describe a pre-derivation embryo haplotyping strategy that we developed in order to maximize the efficiency and minimize the costs of establishing banks of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes match a significant proportion of the population. Using whole genome amplification followed by medium resolution HLA typing using PCR amplification with sequence-specific primers (PCR-SSP), we have typed the parents, embryos and hESC lines from three families as well as our eight clinical grade hESC lines and shown that this technical approach is rapid, reliable and accurate. By employing this pre-derivation strategy where, based on HLA match, embryos are selected for a GMP route on day 3-4 of development, we would have drastically reduced our cGMP laboratory running costs. © 2012 The Authors.

Loading Guys And St Thomas Center For Preimplantation collaborators
Loading Guys And St Thomas Center For Preimplantation collaborators