Gurunanak Institute of Dental Science and Research

Kolkata, India

Gurunanak Institute of Dental Science and Research

Kolkata, India

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Kar S.,Gurunanak Institute of Dental Science and Research | Sarkar S.,Gurunanak Institute of Dental Science and Research
Journal of Clinical and Diagnostic Research | Year: 2014

Introduction: Developmental defects of the enamel (D.D.E.) are changes in the deciduous dentition may lead to aesthetic problems, dental sensitivity and may be predictors of dental caries. The aim of this study was to estimate the prevalence and distribution of D.D.E. in the deciduous dentition of IVF children of West Bengal. Materials and Methods: A cross-sectional study was carried out with a sample of 153 IVF children aged three to five enrolled in institute of reproductive medicine Kolkata West Bengal. One hundred fifty three spontaneously conceived matched controlled children also examined as control group. All of the teeth were examined and the enamel defects were assessed according to the Modified D.D.E Index. The differences were tested for statistical significance by using the Chi-Square test & z-test. Results: The prevalence of D.D.E. was 7.18% in IVF Children and 8.49% in spontaneously conceived children. Diffuse opacities was the common defect found (2.61%) in both group. The most affected teeth were the deciduous maxillary central incisors (12.82% and 13.63% found in IVF and spontaneously conceived group respectively). Defects were observed more frequently in the maxillary arch than in mandibular arch in both groups. Conclusion: There is no significant difference found in IVF & Spontaneously Conceived Children group.


Chandra A.K.,University of Calcutta | Sengupta P.,University of Calcutta | Goswami H.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Indian Journal of Experimental Biology | Year: 2013

The available information on the effect of excess dietary magnesium on male reproduction is inadequate, though consumption of hard water rich in magnesium salt is not uncommon in many geographical areas. The present study has thus been undertaken to evaluate the morphological as well as cytological and functional changes in testis of magnesium administered sexually mature male Wistar rats. Significant increase in the activities of androgenic enzymes viz. {increment}53β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase with concomitant increase in serum testosterone level, followed by progressive development in cytoarchitechture of genital organs, without any significant alteration in quantitative spermatogenesis were observed. The results were more marked in the groups treated for longer duration. The results further suggests that the changes that occurred after excessive magnesium in testis were not for the enhanced adrenocortical activities or for the generation of oxidative stress in reproductive organs, but for the direct action of excess magnesium on male gonads. Magnesium supplementation thus has an apparent beneficial effect on male gonadal system.


Batabyal B.,Gurunanak Institute of Dental Science and Research | Biswas S.,Gurunanak Institute of Dental Science and Research | Mandal B.,I.P.G.M.E. and R.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

Staphylococcus aureus is most frequently isolated pathogen causing bloodstream infections, skin and soft tissue infections and pneumonia. Recently, Staph. aureus have evolved resistance to both synthetic and traditional antibiotics. This study was carried out to isolate pathogenic Staph. aureus from post-operative pus sample, and Imipenem was identified by evaluation of resistance patterns using conventional antibiotics. A total of 66 pus samples from post-operative oral & maxillofacial surgical infections were received in the Department of Microbiology, Gurunanak Institute of Dental Science & Research, Panihati, Kolkata, over a period of one year. Imipenem is a broad spectrum antibiotic widely prescribed in hospital and it is still considered as a better choice against fatal infections. The emergence of antimicrobial resistance against effective antibiotics is a global issue. The objective of study is the surveillance of imipenem against Staphylococcus aureus isolated from post-operative oral & maxillofacial infections. To investigate the present status of antimicrobial resistance against imipenem, 51.5% isolates of Staph. aureus were collected during study period. The in-vitro antimicrobial activity of imipenem was carried out by Disc Diffusion Method (Kirby-Bauer test). 29.5% of the isolates were shown to be imipenem resistant Staph. aureus. It is concluded that the clinical isolates have started developing resistance against imipenem.


Tejo S.K.,Principal Gandhi Dental College | Kumar A.G.,Saraswati Dhanwantari Dental College and Hospital | Kattimani V.S.,Saraswati Dhanwantari Dental College and Hospital | Desai P.D.,Gurunanak institute of dental science and research | And 2 more authors.
Head and Face Medicine | Year: 2012

Background: The introduction of different interocclusal recording materials has put clinicians in dilemma that which material should be used in routine clinical practice for precise recording and transferring of accurate existing occlusal records for articulation of patient's diagnostic or working casts in the fabrication of good satisfactory prosthesis. In the era of developing world of dentistry the different materials are introduced for interocclusal record with different brand names because of this; the utility of the material is confusing for successful delivery of prosthesis with lack of in vitro or in vivo studies which will predict the property of the material with utility recommendations.Purpose of the study: The aim of this multicenter research is to evaluate the time dependent linear dimensional stability of three types of interocclusal recording materials; which gives very clear idea to clinicians in regard to its usage in routine practice and recommendations for usage of the different materials. Also to find out ideal time for articulation of three types of interocclusal recording materials with accuracy.Materials and method: Commercially available and ADA approved Polyether bite registration paste (Ramitec), Poly vinyl siloxane bite registration paste (Jetbite) and Zinc oxide eugenol (ZOE) bite registration paste (Super bite) were used in the study.A stainless steel die was made according to modified American dental Associations (ADA) specification no. 19. Each one of the tested materials were manipulated according to manufacturers' instructions. The materials separated from die, 3-mins after their respective setting time, resulted in disks of standard diameter. Two parallel lines and three perpendicular lines reproduced on the surface. The distance between two parallel lines was measured at different time intervals i.e. 1 hour, 24, 48 and 72 hours by using travelling microscope (magnus) and compared with standard die measurements made according to ADA specification no.19 to find out the dimensional stability of these interocclusal recording materials. Total 120 samples were made for observation and results were subjected to statistical analysis. Statistical analysis was performed using analysis of variance (ANOVA) and then Tukey's Honestly Significant Difference (HSD) test for comparison among groups at the 0.05 level of significance. After statistical analysis of the data, results were obtained and analyzed for interpretation.Results: The results shows significant difference between the dimensional stability of all three material at different intervals with p-value <0.05. Comparatively the polyether bite registration material showed less distortion with good dimensional stability compared to Poly vinyl siloxane bite (Jetbite), Zinc oxide eugenol(ZOE) bite (Super bite) at 1 hour, 24, 48, and 72 hours.Conclusion: The dimensional stability decreased with increase in time and is influenced by both material factor and time factor. Polyether was found to be more dimensionally stable interocclusal recording material, which was followed by Silicone and Zinc oxide eugenol (ZOE). The dimensional stability of Polyether was good. Zinc oxide eugenol is dimensionally more unstable when compared with polyether and polyvinyl siloxane. We recommend that the polyether interocclusal records must be articulated within 48 hours and Polyvinylsiloxane interocclusal records must be articulated within 24 hours and the ZOE should be articulated within 1 hour to get a correct restoration to have very minimum distortion and maximum satisfaction without failure of prosthesis. © 2012 Tejo et al.; licensee BioMed Central Ltd.


Chandra A.K.,University of Calcutta | Choudhury S.R.,University of Calcutta | De N.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Indian Journal of Experimental Biology | Year: 2011

Green tea, prepared from the steamed and dried leaves of the shrub Camellia sinensis, is known for its antioxidant andanti-carcinogenic effects. However, its effects on male gonadal functions have not been explored adequately and the presentinvestigation has been undertaken to evaluate the effect of green tea extract on gonads of adult male albino rats. Results of invivo studies showed that green tea extract (GTE) at mild (1.25 g%, Ξ 5 cups of tea/day), moderate (2.5 g%, Ξ10 cups oftea/day) and high (5.0 g%, Ξ 20 cups of tea/day) doses, for a period of 26 days, altered morphology and histology of testisand accessory sex organs. A significant dose-dependent decrease in the sperm counts, inhibited activities of testicular Δ5 3β-and 17β-hydroxysteroid dehydrogenase (Δ 5-3β-HSD and 17β-HSD respectively) and decreased serum testosterone levelwere noticed. Significant increase in serum LH level was observed after moderate and high doses; serum FSH level alsoincreased but not significantly. Histopathological examination showed inhibition of spermatogenesis evidenced bypreferential loss of matured and elongated spermatids. Results of this study showed that GTE at relatively high dose maycause impairment of both the morphological and normal functional status of testis in rodents and thus its consumption atrelatively high doses raises concern on male reproductive function in spite of its other beneficial effects.


Chandra A.K.,University of Calcutta | Sengupta P.,University of Calcutta | Goswami H.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Molecular and Cellular Biochemistry | Year: 2012

Calcium is essential for functioning of different systems including male reproduction. However, it has also been reported as chemo-castrative agent. The study has been undertaken to elucidate the effect of excessive dietary calcium on male reproductive system in animals with possible action. Adult male healthy rats fed CaCl2 at different doses (0.5, 1.0 and 1.5 g%) in diet for 13 and 26 days to investigate reproductive parameters as well as the markers of oxidative stress. Significant alteration was found (P<0.05) in testicular and accessory sex organs weight, epididymal sperm count, testicular steroidogenic enzyme (Δ5 3β-HSD and 17β-HSD) activities, serum testosterone, LH, FSH, LPO, activities of antioxidant enzymes, testicular histoarchitecture along with adrenal Δ5 3β-HSD activity with corticosterone level in dose- and time-dependent manner. Overall observations suggest that excessive dietary calcium enhances the generation of freeradicals resulting in structural and functional disruption of male reproduction. © Springer Science+Business Media, LLC. 2011.


Chandra A.K.,University of Calcutta | Chatterjee A.,University of Calcutta | Ghosh R.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Food and Chemical Toxicology | Year: 2010

Excess chromium (Cr) exposure is associated with various pathological conditions including reproductive dysfunction. Generation of oxidative stress is one of the plausible mechanisms behind Cr induced cellular deteriorations. The efficacy of vitamin E to combat Cr induced oxidative damage in adult rat testis has investigated in the current study. Adult male rats exposed to hexavalent Cr (intraperitoneal injection with 0.4 mg K2Cr2O7/kg bw/day) for 26 days resulted in decreased accessory sex organs weight compared to controls. Development of oxidative stress in testis was evidenced by increased lipid peroxidation along with decreased superoxide dismutase (SOD) and catalase activities than control animals. Marked reduction in the activities of testicular steroidogenic enzymes; Δ53β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, serum testosterone and Leutinizing Hormone (LH) levels were observed. However significant increase in serum Follicle Stimulating Hormone (FSH) level was observed with Cr treated group. Histological evaluation of testis revealed degeneration of stage VII spermatogenic cycle along with decrease in epithelial cell height in epididymis and seminiferous tubules; number of different germ cells per seminiferous tubule and seminiferous tubular diameter reduced after Cr exposure. Simultaneous oral supplementation of vitamin E (50 mg/kg bw/day) in Cr exposed rats showed less oxidative damage and restored the otherwise altered testicular activities. Epididymal sperm number was also restored in vitamin E-supplemented group than Cr induced rats. This study implicates vitamin E as a possible protective agent against Cr induced spermatogenic and steroidogenic alteration. © 2010 Elsevier Ltd. All rights reserved.


Chandra A.K.,University of Calcutta | Ghosh R.,University of Calcutta | Chatterjee A.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Toxicology Mechanisms and Methods | Year: 2010

Vanadium is a well recognized industrial hazard known to adversely affect male reproductive functions. The intricate mechanistic aspects of this metal and the role of oxidative stress in the deterioration of testicular functions are investigated in the current study. The experiment also focused on the effects of testosterone propionate in testicular and sperm functions in the rat intoxicated with vanadate. Vanadium exposure resulted in a more prominent spermatogenic arrest and consistently abolished the conversion of round to mature spermatids along with decreased epididymal sperm number and increased percentage of abnormal sperm. This is followed by a precipitous decline in the level of serum testosterone and gonadotropins and consequently the testicular steroidogenic and antioxidant enzymes were inhibited. Vanadium induces degeneration in the genital organs of rats and exhibits high indices of lipid oxidative damage. In response to exogenous testosterone propionate (TP) administration, spermatogonial cell populations remained suppressed, while the spermatogenesis was restored quantitatively. In contrast, the hormone treatment had no effect on the dramatically decreased serum FSH level after vanadate treatment. Moreover, TP could ameliorate the toxicity, as indicated by decreased testicular lipid peroxidation with marginal but significant increase in the activities of all the measured enzymes following vanadate-treatment. Taken together all these studies establish that vanadium is a testicular toxicant that perturbs the male reproductive system adversely. However, hormone replacement therapy by testosterone propionate may provide partial protection. The results suggest the feasibility of using endocrine regimens to impede deleterious effects of vanadium on the male reproductive system. © 2010 Informa UK Ltd.


PubMed | Gurunanak Institute of Dental science and Research
Type: Journal Article | Journal: The Journal of prosthetic dentistry | Year: 2016

No cost-effective method of ascertaining bone density from 2-dimensional radiographic images is currently available for dental implants before surgery.The purpose of this invivo study was to use digital panoramic radiology and dental computed tomography (CT) to evaluate the bone density of specific points in the jaw near the tooth-bearing areas. The objective was to determine whether digital panoramic radiology can be used in assessing bone density as an alternative to a more expensive and complex dental CT.This study involved determining bone densities at predetermined anatomic landmarks near tooth-bearing areas of the jaws of 20 participants, using digital panoramic radiology in gray-level scale with a lead step wedge. Subsequently, the bone densities of the same points were determined in Hounsfield units (Hu) with dental CT. The data collected after interpretation of the panoramic radiograph and CT were tabulated and analyzed statistically.Bone density measured using CT correlated with the first 3 steps of (A, B, and C) the digital scale of gray. Further analysis conducted using the Mann-Whitney U test showed a significant association between step A to detect D4 bone, step B to detect D3 bone, and step C to detect D2 type bone.The digital scale of gray obtained from a lead step wedge can be effectively used with digital orthopanoramic radiology to assess bone density before the placement of implants, but with certain restrictions.


PubMed | Sardar Patel Post Graduate Institute of Dental and Medical science and Gurunanak Institute of Dental science and Research
Type: Journal Article | Journal: Contemporary clinical dentistry | Year: 2016

Laser first came into light in 1960 and had been used extensively in various fields of medicine. Laser has been experimented in the various dental field, and its utility is being recognized and established well in the dentistry. Lasers are widely used for a number of procedures such as cavity preparation, scaling, and root planning, surgical procedures like excision of soft tissue growths, etc., Improved healing, hemostasis, and sutureless excisions are some of the many advantages of laser over conventional treatment modalities. It is because of these advantages that laser is becoming more and more popular as a treatment option in various aspects of dentistry. We hereby present a case report, where we have used diode laser for surgical management of a proliferative verrucous leukoplakia (PVL), because of its many advantages over conventional methods. It presents very specific characteristics, mainly a more aggressive biological behavior than other forms of leukoplakia expressed by: A tendency toward multifocality (field cancerization); a high prospect of recurrence; and a high rate of malignant transformation, which can range between 40% and 100% in a follow-up period of 4.4-11.6 years. In this case, we evaluated the advantages of diode laser for the treatment of verrucous leukoplakia, where the results that we obtained were excellent. The patient had come for evaluation till the time of complete healing.

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