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Chandra A.K.,University of Calcutta | Sengupta P.,University of Calcutta | Goswami H.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Indian Journal of Experimental Biology | Year: 2013

The available information on the effect of excess dietary magnesium on male reproduction is inadequate, though consumption of hard water rich in magnesium salt is not uncommon in many geographical areas. The present study has thus been undertaken to evaluate the morphological as well as cytological and functional changes in testis of magnesium administered sexually mature male Wistar rats. Significant increase in the activities of androgenic enzymes viz. {increment}53β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase with concomitant increase in serum testosterone level, followed by progressive development in cytoarchitechture of genital organs, without any significant alteration in quantitative spermatogenesis were observed. The results were more marked in the groups treated for longer duration. The results further suggests that the changes that occurred after excessive magnesium in testis were not for the enhanced adrenocortical activities or for the generation of oxidative stress in reproductive organs, but for the direct action of excess magnesium on male gonads. Magnesium supplementation thus has an apparent beneficial effect on male gonadal system.


Sukul B.,NRS Medical College and Hospital | Deb U.,R Ahmed Dental College and Hospital | Ghosh S.,Gurunanak Institute of Dental Science and Research
Journal of the Indian Medical Association | Year: 2010

Dentistry has much to offer law enforcement in the detection and solution of crime or in civil proceedings. Forensic dental fieldwork requires an interdisciplinary knowledge of dental science and forensic science. Most often the role of the forensic odontologist is to establish a person's identity. Teeth, with their physiologic variations, pathoses and effects of therapy, record information that remains throughout life and beyond. The teeth may also be used as weapons and, under certain circumstances, may leave information about the identity of the culprit. Forensic odontology has an important role in the recognition of abuse among persons of all ages. Dental professionals have a major role to play in keeping accurate dental records and providing all necessary information so that legal authorities may recognise malpractice, negligence, fraud or abuse, and identify unknown human. Forensic odontology involves the management, examination, evaluation and presentation of dental evidence in criminal or civil proceedings, all in the interest of justice. The forensic odontologist assists legal authorities by examining dental evidence in different situations.


Chandra A.K.,University of Calcutta | Sengupta P.,University of Calcutta | Goswami H.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Molecular and Cellular Biochemistry | Year: 2012

Calcium is essential for functioning of different systems including male reproduction. However, it has also been reported as chemo-castrative agent. The study has been undertaken to elucidate the effect of excessive dietary calcium on male reproductive system in animals with possible action. Adult male healthy rats fed CaCl2 at different doses (0.5, 1.0 and 1.5 g%) in diet for 13 and 26 days to investigate reproductive parameters as well as the markers of oxidative stress. Significant alteration was found (P<0.05) in testicular and accessory sex organs weight, epididymal sperm count, testicular steroidogenic enzyme (Δ5 3β-HSD and 17β-HSD) activities, serum testosterone, LH, FSH, LPO, activities of antioxidant enzymes, testicular histoarchitecture along with adrenal Δ5 3β-HSD activity with corticosterone level in dose- and time-dependent manner. Overall observations suggest that excessive dietary calcium enhances the generation of freeradicals resulting in structural and functional disruption of male reproduction. © Springer Science+Business Media, LLC. 2011.


Chandra A.K.,University of Calcutta | Choudhury S.R.,University of Calcutta | De N.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Indian Journal of Experimental Biology | Year: 2011

Green tea, prepared from the steamed and dried leaves of the shrub Camellia sinensis, is known for its antioxidant andanti-carcinogenic effects. However, its effects on male gonadal functions have not been explored adequately and the presentinvestigation has been undertaken to evaluate the effect of green tea extract on gonads of adult male albino rats. Results of invivo studies showed that green tea extract (GTE) at mild (1.25 g%, Ξ 5 cups of tea/day), moderate (2.5 g%, Ξ10 cups oftea/day) and high (5.0 g%, Ξ 20 cups of tea/day) doses, for a period of 26 days, altered morphology and histology of testisand accessory sex organs. A significant dose-dependent decrease in the sperm counts, inhibited activities of testicular Δ5 3β-and 17β-hydroxysteroid dehydrogenase (Δ 5-3β-HSD and 17β-HSD respectively) and decreased serum testosterone levelwere noticed. Significant increase in serum LH level was observed after moderate and high doses; serum FSH level alsoincreased but not significantly. Histopathological examination showed inhibition of spermatogenesis evidenced bypreferential loss of matured and elongated spermatids. Results of this study showed that GTE at relatively high dose maycause impairment of both the morphological and normal functional status of testis in rodents and thus its consumption atrelatively high doses raises concern on male reproductive function in spite of its other beneficial effects.


Chandra A.K.,University of Calcutta | Chatterjee A.,University of Calcutta | Ghosh R.,University of Calcutta | Sarkar M.,Gurunanak Institute of Dental Science and Research
Food and Chemical Toxicology | Year: 2010

Excess chromium (Cr) exposure is associated with various pathological conditions including reproductive dysfunction. Generation of oxidative stress is one of the plausible mechanisms behind Cr induced cellular deteriorations. The efficacy of vitamin E to combat Cr induced oxidative damage in adult rat testis has investigated in the current study. Adult male rats exposed to hexavalent Cr (intraperitoneal injection with 0.4 mg K2Cr2O7/kg bw/day) for 26 days resulted in decreased accessory sex organs weight compared to controls. Development of oxidative stress in testis was evidenced by increased lipid peroxidation along with decreased superoxide dismutase (SOD) and catalase activities than control animals. Marked reduction in the activities of testicular steroidogenic enzymes; Δ53β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, serum testosterone and Leutinizing Hormone (LH) levels were observed. However significant increase in serum Follicle Stimulating Hormone (FSH) level was observed with Cr treated group. Histological evaluation of testis revealed degeneration of stage VII spermatogenic cycle along with decrease in epithelial cell height in epididymis and seminiferous tubules; number of different germ cells per seminiferous tubule and seminiferous tubular diameter reduced after Cr exposure. Simultaneous oral supplementation of vitamin E (50 mg/kg bw/day) in Cr exposed rats showed less oxidative damage and restored the otherwise altered testicular activities. Epididymal sperm number was also restored in vitamin E-supplemented group than Cr induced rats. This study implicates vitamin E as a possible protective agent against Cr induced spermatogenic and steroidogenic alteration. © 2010 Elsevier Ltd. All rights reserved.

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