Guru nanak Institute of Pharmacy

Ibrahimpatnam, India

Guru nanak Institute of Pharmacy

Ibrahimpatnam, India
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Sharma D.,Rayat Bahra Institute of Pharmacy | Singh G.,Guru Nanak Institute of Pharmacy | Kumar D.,Chandigarh Institute of Microbial Technology | Singh M.,Quantum Solutions
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: To develop a simple, rapid, accurate, precise and economical spectroscopic method for the simultaneous determination of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation and validate as per ICH guidelines.Methods: In this method, the 6.8 pH phosphate buffer was selected for the developing spectral characteristics of the three drugs. The overlay spectra of Salbutamol Sulphate, Ambroxol hydrochloride and Cetirizine hydrochloride were resolved by making the use of simultaneous equation method based on measurement of absorbance at three wavelengths.Results: The λmaxof Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine hydrochloride were found to be 276 nm, 244 nm and 230 nm. The method obeyed Beer Lambert’s law in the concentration range of 10-100 μg/ml for SAL, 2- 18 μg/ml for AMB HCl and 2-20 μg/ml for CET HCl. The high values of correlation coefficient (R) indicated good linearity of calibration plot for the drugs. Result of percentage recovery study confirms the accuracy of proposed method. Percentage RSD for precision and accuracy of the method was found to be less than 2%. LOD values for SAL, AMB, and CET were found to be 0.523 𝜇𝜇g/mL, 0.450 𝜇𝜇g/mL and 0.457 𝜇𝜇g/mL, respectively. LOQ values for SAL, AMB, and CET were found to be 1.372 𝜇𝜇g/mL, 1.424 𝜇𝜇g/mL and 1.386 𝜇𝜇g/mL, respectively.Conclusion: A rapid, economical, accurate, precise and reproducible simultaneous equation spectroscopic method was developed and validated. The proposed method can be employed for routine analysis of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation. ©.2014, International Journal of Pharmacy and Pharmaceutical Science. All Right Reserved.

Patil S.H.,University of Rajasthan | Sreenivas S.A.,Guru Nanak Institute of Pharmacy | Deshmukh P.V.,Guru Nanak Institute of Pharmacy | Srikanth M.,Guru Nanak Institute of Pharmacy
International Journal of Drug Development and Research | Year: 2012

The present study was designed to investigate antidiabetic & hypolipidemic potential of alcoholic extract of Alocasia indica Schott. leaves (Araceae) in streptozotocin induced diabetic rats. Diabetes was induced by single intraperitoneal injection of streptozotocin (60 mg kg-1). Graded doses (200 & 400 mg kg-1) of test extracts were studied in both normal & streptozotocin induced diabetic rats for the period of 21 days. Glibenclamide (10 mg kg-1) was used as standard drug. Blood glucose levels & body weight was monitored at specific intervals and lipid profile parameters such as serum cholesterol, serum triglyceride & high density lipoprotein (HDL) were also assessed in experimental animals. The statistical data indicated significant increase in body weight & decrease in blood glucose level & serum lipid profiles (cholesterol & triglyceride) in test extract treated animals. These results could explain the basis for the use of this plant extract to manage serum glucose level and lipidemia associated with diabetes mellitus. © 2012 IJDDR, S. H. Patil et al.

Mandade R.,Sn Institute Of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy
International Journal of Pharmacology | Year: 2011

Medicinal plants play a major role in the management of Diabetes mellitus especially in developing countries. The present study investigated the possible therapeutic effects of Hibiscus rosasinensis (H. rosasinensis) extract on certain biochemical markers in Streptozotocin (STZ)-induced diabetes mellitus in rats. The effects of an aqueous ethanolic extract of H. rosasinensis Aerial part on blood glucose, albumin, albumin/globulin ratio, urea, insulin, C-peptide, uric acid and creatinine and the activities of diagnostic marker enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase were examined in the plasma, liver and kidney tissues of control and experimental groups. Oral administration of H. rosasinensis (500 mg kg-1) aqueous extract to diabetic rats for 4 weeks significantly reduced blood glucose, urea, uric acid and creatinine but increased the activities of insulin, C-peptide, albumin, albumin/globulin ratio and restored all marker enzymes to near control levels. The present results shown that H. rosasinensis extract has an antihyperglycaemic effect and consequently may alleviate liver and renal damage associated with STZ-induced diabetes mellitus in rats. © 2011 Asian Network for Scientific Information.

Samal H.B.,Guru Nanak Institute of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy | Dey S.,Guru Nanak Institute of Pharmacy | Sharma H.,Sandoz Pvt Ltd
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

The investigation was concerned with design and characterization of oral Sustained release matrix tablets of Zidovudine (AZT) in order to improve efficacy and better patient compliance. Matrix tablets were prepared by Wet granulation method using various proportions of hydrophilic polymers like Sodium CMC, HPMC, Eudragit-Ll55, & Xanthan gum along or in combination with hydrophobic polymer ethyl cellulose. In vitro drug release studies were performed using USP type II apparatus (rotary paddle type). The release kinetics was analyzed using Zero order, First order, Higuchi and Hixson Crowell. Compatibility of drug with various formulations excipients was also studied. In this study Formulations X3, X4, X5, X7, X9, X12 and X14 shows zero-order kinetic of release with 80% to 97% of drug released. As these formulations containing a combination of two polymer i.e. Xanthan gum, combine with EC, Sodium CMC, HPMC in the different ratios. But in presence of sodium CMC in the formulation X4, X9, X14, E3, E7 and E11, the drug released in 12 hour was in the range of 75% to 85% and shows zero-order kinetics. The formulation X4 & E3 containing Na CMC with Xanthan gum and EC respectively in the ratio 1: 1 gave more sustaining action i.e. 79.16% and 65.21% respectively in 12 hour. From the above study it was concluded that presence of sodium CMC gives zero-order release kinetics and the linearity ranges from 0.990 to 0.996. It has also good drug entrapment efficiency ranges from 96 to 106% of drug. Formulation containing sodium CMC with Xanthan gum and EC gives sustained release of drug more than 12hrs.

Mandade R.,Sn Institute Of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy | Sakarkar D.M.,Sn Institute Of Pharmacy | Choudhury A.,Shree Dhanvantary Pharmaceutical Analysis and Research Center
African Journal of Pharmacy and Pharmacology | Year: 2011

Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. In the present study, the antioxidant properties of crude extract of Hibiscus rosasinensis were examined, using different in vitro analytical methodologies, such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS + radical cation) radical cation scavenging activity and superoxide anion radical scavenging by riboflavin-methionine-illuminate system. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and α-tocopherol were used as the reference antioxidant radical scavenger compounds. The crude extract inhibited 94.58% peroxidation of linoleic acid emulsion at 20 μg/ml concentration, while the standard antioxidants BHA, BHT and α-tocopherol indicated an inhibition of 93.75, 96.66 and 83.33% at 60 μg/ml concentration, respectively. The hydrogen peroxide radical, DPPH radical, ABTS + radical cation(s) and superoxide anion radical scavenging activities of crude extract were also compared to BHA, BHT and α-tocopherol as references antioxidant compounds. The present study shows that the crude extract is an effective natural antioxidant component. © 2011 Academic Journals.

Jadhav J.K.,Sudhakarrao Naik Institute of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The purpose of this research work was to develop and evaluate matrix-type Transdermal drug delivery system containing Indomethacin with different ratios of hydrophilic and hydrophobic polymeric combinations by the solvent evaporation technique. The physicochemical compatibility of the drug and the polymers were studied by infrared spectroscopy. The results suggested no physicochemical incompatibility between the drug and the polymers. Transdermal patch formulations consists of Hydroxypropyl methylcellulose E5 and Ethyl cellulose in the ratios of 10:0, 0:10, 1:9, 2:8,3:7, 4:6, 5:5, 6:4, 7:3, 8:2 and 9:1 respectively were prepared. All formulations carried 20% v/w of dimethyl sulfoxide as penetration enhancer and 20% v/w of dibutyl phthalate as plasticizer in chloroform and methanol (1:1) as solvent system. The prepared Transdermal patches were evaluated for in vitro release, moisture absorption, moisture loss and mechanical properties. The diffusion studies were performed by using Franz diffusion cells. The formulation, F8 with combination of polymers (3:2) showed maximum release of 75.28% in 24 h. Hence, it can be reasonably concluded that Indomethacin can be formulated into the Transdermal matrix type patches to sustain its release characteristics.

Verma S.,Rayat Bahra Institute of Pharmacy | Jain A.K.,Guru Nanak Institute of Pharmacy
Artificial Cells, Nanomedicine and Biotechnology | Year: 2015

Human immunodeciency virus (HIV) infection has become devastating in last a few years. Nearly 7400 new infection cases are coming every day. Highly active antiretroviral therapy (HAART), which involves combination of at least three antiretroviral (ARV) drugs, has been used to extend the life span of the HIV-infected patients. HAART has played an important role to reduce mortality rate in the developed countries but in the developing countries condition is still worst with millions of people being infected by this disease. For the improvement of the situation, nanotechnology-based drug system has been explored for the HIV therapeutics. Nanosystems used for HIV therapeutics offer some unique advantage like enhancement of bioavailability, water solubility, stability, and targeting ability of ARV drugs. Main nanotechnology-based systems explored for HIV therapeutics are liposomes, nanoparticles, niosomes, polymeric micelles, and dendrimers. Present manuscript reviews conventional method of HIV therapeutics and recent advances in the eld of nanotechnology-based systems for treatment of HIV-AIDS. © 2014 Informa Healthcare USA, Inc.

Bhardwaj A.,Punjab Technical University | Grobler A.,North West University South Africa | Jain A.K.,Guru Nanak Institute of Pharmacy | Mehta A.,North West University South Africa
Current Drug Delivery | Year: 2016

Background: Mycobacterium tuberculosis (M. TB) remains the prime cause of bacterial mortality and morbidity world-wide. Therefore, effective delivery and targeting of drug to the cellular tropics is essentially required to generate significant results for tuberculosis treatment. The aim of the present study was to develop and characterize ligand anchored pH sensitive liposomes (TPSL) as dry powder inhaler for the targeted delivery of drugs in the target site i.e. lungs. Method: Ligand anchored PSL (TPSL) was prepared by thin film hydration for the combined delivery of Isoniazid (INH) and Ciprofloxacin HCl (CIP HCl) using 4-aminophenyl-α-D mannopyranoside (Man) as surface functionalized ligand and characterized using different parameters. Results: It was observed that size of the ligand anchored liposomes (TPSL) was slightly more than the non-ligand anchored liposomes (PSL). Drug release was studied at different pH for 24 hrs and it was observed that liposomes exhibited slow release at alkaline pH (58-64%) as compared to macrophage pH (81-87%) where it increased dramatically due to the destabilization of pH sensitive liposome (PSL). In vitro cellular uptake study showed that much higher concentration was achieved in the alveolar macrophage using ligand anchored liposomes as compared to its counterpart. In vivo study showed that maximum drug accumulation was achieved in the lung by delivering drug using ligand anchored PSL as compared to conventional PSL. Conclusion: It was concluded that ligand anchored pH sensitive liposome is one of the promising systems for the targeted drug therapy in pulmonary tuberculosis. © 2016 Bentham Science Publishers.

Mandade R.J.,S N Institute Of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy | Sakarkar D.M.,S N Institute Of Pharmacy | Choudhury A.,Shree Dhanvantary college pharmacy
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To explore the effects of extract of Hibiscus rosasinensis (H. rosasinensis) on the volume, free and total acidity of gastric secretion induced by carbachol. Methods: Animals were kept on fasting for 48 h, then the pylorus of each animal was ligated. They were randomly divided into 5 groups and treated by carbachol at 600 μg/kg. Then animals in group II - V were treated by H. rosasinensis extract at 250 and 500 mg/kg body weight, cimetidine at 2.5 mg/kg and verapamil at 10 mg/kg body weight intraperitoneally, respectively. The volume, free and total acidity of gastric secretion were observed and compared. Results: It was found that the extract significantly reduced the volume, free and total acidity of gastric secretion (P<0.01). These reductions were comparable to cimetidine and verapamil. And the reduction in the volume and free acidity were more significant in cimetidine and verapamil treated group indicating that cimetidine and verapamil were more effective. Conclusions: The extract of H. rosasinensis can reduced the volume, free and total acidity of gastric secretion, and can be used effectively in the treatment of peptic ulcer. © 2011 Hainan Medical College.

Mandade R.J.,Maharashtra Institute of Pharmacy | Choudhury A.,Guru Nanak Institute of Pharmacy | Harsulkar A.,Maharashtra Institute of Pharmacy | Wakade R.,Maharashtra Institute of Pharmacy
Indian Journal of Pharmacology | Year: 2011

Objectives: The objective of the study was to investigate the effect of the leaf extract of Rosa canina L. against experimental diarrhea induced by castor oil in rodents. Materials and Methods: The methanol extract of Rosa canina L. (30 and 60 mg/kg body weight) was administered orally to two groups of mice (five animals per group) in order to evaluate the activity of the extract against the castor oil-induced diarrhea model in mice. Two other groups received normal saline and diphenoxylate (5 mg/kg) as positive control. The effect of the extract on intestinal transit and castor oil-induced intestinal fluid accumulation (enteropooling) was assessed. The effects of the extract on the isolated rabbit jejunum and on the isolated rat ileum were studied. Results: The preliminary phytochemical screening of the leaf extract of Rosa Canina L. revealed the presence of alkaloids, flavonoids, glycosides, saponins, and volatile oil. Intraperitoneal LD50 of the extract was found to be 455.19 ± 23 mg/kg in mice. The antidiarrheal effect of the methanolic extract exhibited a concentration-dependent inhibition of the spontaneous pendular movement of the isolated rabbit jejunum and inhibited acetylcholine-induced contraction of the rat ileum. A dose-dependent decrease in gastrointestinal transit was observed with extracts (30 and 60 mg/kg), which also protected mice against castor oil-induced diarrhea and castor oil-induced fluid accumulation, respectively. Conclusions: The presence of some of the phytochemicals in the leaf extract may be responsible for the observed effects, and also the basis for its use in traditional medicine as an antidiarrheal drug.

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