Ibrahimpatnam, India
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Sharma D.,Rayat Bahra Institute of Pharmacy | Singh G.,Guru Nanak Institute of Pharmacy | Kumar D.,Chandigarh Institute of Microbial Technology | Singh M.,Quantum Solutions
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: To develop a simple, rapid, accurate, precise and economical spectroscopic method for the simultaneous determination of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation and validate as per ICH guidelines.Methods: In this method, the 6.8 pH phosphate buffer was selected for the developing spectral characteristics of the three drugs. The overlay spectra of Salbutamol Sulphate, Ambroxol hydrochloride and Cetirizine hydrochloride were resolved by making the use of simultaneous equation method based on measurement of absorbance at three wavelengths.Results: The λmaxof Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine hydrochloride were found to be 276 nm, 244 nm and 230 nm. The method obeyed Beer Lambert’s law in the concentration range of 10-100 μg/ml for SAL, 2- 18 μg/ml for AMB HCl and 2-20 μg/ml for CET HCl. The high values of correlation coefficient (R) indicated good linearity of calibration plot for the drugs. Result of percentage recovery study confirms the accuracy of proposed method. Percentage RSD for precision and accuracy of the method was found to be less than 2%. LOD values for SAL, AMB, and CET were found to be 0.523 𝜇𝜇g/mL, 0.450 𝜇𝜇g/mL and 0.457 𝜇𝜇g/mL, respectively. LOQ values for SAL, AMB, and CET were found to be 1.372 𝜇𝜇g/mL, 1.424 𝜇𝜇g/mL and 1.386 𝜇𝜇g/mL, respectively.Conclusion: A rapid, economical, accurate, precise and reproducible simultaneous equation spectroscopic method was developed and validated. The proposed method can be employed for routine analysis of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation. ©.2014, International Journal of Pharmacy and Pharmaceutical Science. All Right Reserved.


Kumar L.,Punjab Technical University | Verma S.,Punjab Technical University | Jain A.K.,Punjab Technical University | Jain A.K.,Guru Nanak Institute of Pharmacy
Artificial Cells, Nanomedicine and Biotechnology | Year: 2017

The body is protected against the external environment by the skin due to its physical barrier nature. Stratum corneum composed of corneocytes surrounded by lipid region performs a major barrier function as it lies in the uppermost area of skin. Alteration in barrier function, increase in permeability, and disorganization of stratum corneum represent diseased skin. Drugs applied to the diseased skin should induce a local effect at the site of application or area close to it along with cutaneous absorption rather than percutaneous absorption. Conventional formulations like ointments, gels, and creams suffer from the drawback of limited local activity. For the enhancement of drug penetration and localization of the drug at the site of action approaches explored are liposomes, niosomes, ethosomes microparticles, and solid lipid nanoparticles. Vesicles composed of fatty acids like oleic acid and linoleic acid represent the new approach used for transdermal penetration and localization. In this review article, our major aim was to explore the applications of fatty acid vesicles for transdermal delivery of various bioactives. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Verma S.,Rayat Bahra Institute of Pharmacy | Jain A.K.,Guru Nanak Institute of Pharmacy
Artificial Cells, Nanomedicine and Biotechnology | Year: 2015

Human immunodeciency virus (HIV) infection has become devastating in last a few years. Nearly 7400 new infection cases are coming every day. Highly active antiretroviral therapy (HAART), which involves combination of at least three antiretroviral (ARV) drugs, has been used to extend the life span of the HIV-infected patients. HAART has played an important role to reduce mortality rate in the developed countries but in the developing countries condition is still worst with millions of people being infected by this disease. For the improvement of the situation, nanotechnology-based drug system has been explored for the HIV therapeutics. Nanosystems used for HIV therapeutics offer some unique advantage like enhancement of bioavailability, water solubility, stability, and targeting ability of ARV drugs. Main nanotechnology-based systems explored for HIV therapeutics are liposomes, nanoparticles, niosomes, polymeric micelles, and dendrimers. Present manuscript reviews conventional method of HIV therapeutics and recent advances in the eld of nanotechnology-based systems for treatment of HIV-AIDS. © 2014 Informa Healthcare USA, Inc.


Gadapuram T.K.,Guru Nanak Institute of Pharmacy | Murthy J.S.N.,Vignana Bharathi Institute of Technology | Rajannagari R.R.,Malla Reddy Pharmacy College | Kandati V.,Malla Reddy Pharmacy College | Shukla R.,Amity University
Journal of Basic and Clinical Physiology and Pharmacology | Year: 2013

Background: Cocculus hirsutus, commonly known as broom creeper, belongs to the family Menispermaceae. It is widely used in folk medicine to treat leprosy, skin diseases, dyspepsia, etc. Hence, an effort has been made to investigate the nephroprotective potential of C. hirsutus. Methods: The nephroprotective activity of ethanolic C. hirsutus leaf extract (ECHE) in the 5/6 nephrectomized rat model was investigated. Different parameters like postoperative survival rate, change in body weight and levels of red blood corpuscles (RBCs), hemoglobin (Hb), triglyceride, cholesterol, creatinine, urea and uric acid were estimated in experimental rats. Results: The findings revealed the postoperative survival rate of rats in the investigated novel method to be 100%. Change in the body weight of ECHE-treated groups I and II was found to be 32 and 30 g, respectively. These values suggested that ECHE treatment normalized the elevated body weight levels in experimental rats. Furthermore, ECHE treatment normalized the decreased RBC levels and the elevated Hb, triglyceride, cholesterol, creatinine, urea and uric acid levels in experimental rats. Conclusions: The results indicate that C. hirsutus has strong nephroprotective activity. However, further scrutiny is essential for isolation and characterization of the active components that can be employed to allay various human maladies.


Dey S.,Guru Nanak Institute of Pharmacy | Reddy Y.V.,Guru Nanak Institute of Pharmacy | Reddy T.,Guru Nanak Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2010

The present study describes a simple, accurate, precise and cost effective UV-VIS Spectrophotometric method for the estimation of Atazanavir, an anti-HIV drug, in bulk and pharmaceutical dosage form. The solvent used was methanol and the λmax or the absorption maxima of the drug was found to be 250nm. A linear response was observed in the range of 10-50μg/ml with a regression coefficient of 0.999. The method was then validated for different parameters as per the ICH (International Conference for Harmonization) guidelines. This method can be used for the determination of Atazanavir in quality control of formulation without interference of the excipients. Atazanavir sulphate was subjected to stress degradation under different conditions recommended by ICH. The samples so generated were used for degradation studies using the developed method.


Bhardwaj A.,Punjab Technical University | Grobler A.,North West University South Africa | Jain A.K.,Guru Nanak Institute of Pharmacy | Mehta A.,North West University South Africa
Current Drug Delivery | Year: 2016

Background: Mycobacterium tuberculosis (M. TB) remains the prime cause of bacterial mortality and morbidity world-wide. Therefore, effective delivery and targeting of drug to the cellular tropics is essentially required to generate significant results for tuberculosis treatment. The aim of the present study was to develop and characterize ligand anchored pH sensitive liposomes (TPSL) as dry powder inhaler for the targeted delivery of drugs in the target site i.e. lungs. Method: Ligand anchored PSL (TPSL) was prepared by thin film hydration for the combined delivery of Isoniazid (INH) and Ciprofloxacin HCl (CIP HCl) using 4-aminophenyl-α-D mannopyranoside (Man) as surface functionalized ligand and characterized using different parameters. Results: It was observed that size of the ligand anchored liposomes (TPSL) was slightly more than the non-ligand anchored liposomes (PSL). Drug release was studied at different pH for 24 hrs and it was observed that liposomes exhibited slow release at alkaline pH (58-64%) as compared to macrophage pH (81-87%) where it increased dramatically due to the destabilization of pH sensitive liposome (PSL). In vitro cellular uptake study showed that much higher concentration was achieved in the alveolar macrophage using ligand anchored liposomes as compared to its counterpart. In vivo study showed that maximum drug accumulation was achieved in the lung by delivering drug using ligand anchored PSL as compared to conventional PSL. Conclusion: It was concluded that ligand anchored pH sensitive liposome is one of the promising systems for the targeted drug therapy in pulmonary tuberculosis. © 2016 Bentham Science Publishers.


Mandade R.J.,S N Institute Of Pharmacy | Sreenivas S.A.,Guru Nanak Institute of Pharmacy | Sakarkar D.M.,S N Institute Of Pharmacy | Choudhury A.,Shree Dhanvantary college pharmacy
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To explore the effects of extract of Hibiscus rosasinensis (H. rosasinensis) on the volume, free and total acidity of gastric secretion induced by carbachol. Methods: Animals were kept on fasting for 48 h, then the pylorus of each animal was ligated. They were randomly divided into 5 groups and treated by carbachol at 600 μg/kg. Then animals in group II - V were treated by H. rosasinensis extract at 250 and 500 mg/kg body weight, cimetidine at 2.5 mg/kg and verapamil at 10 mg/kg body weight intraperitoneally, respectively. The volume, free and total acidity of gastric secretion were observed and compared. Results: It was found that the extract significantly reduced the volume, free and total acidity of gastric secretion (P<0.01). These reductions were comparable to cimetidine and verapamil. And the reduction in the volume and free acidity were more significant in cimetidine and verapamil treated group indicating that cimetidine and verapamil were more effective. Conclusions: The extract of H. rosasinensis can reduced the volume, free and total acidity of gastric secretion, and can be used effectively in the treatment of peptic ulcer. © 2011 Hainan Medical College.


Mandade R.J.,Maharashtra Institute of Pharmacy | Choudhury A.,Guru Nanak Institute of Pharmacy | Harsulkar A.,Maharashtra Institute of Pharmacy | Wakade R.,Maharashtra Institute of Pharmacy
Indian Journal of Pharmacology | Year: 2011

Objectives: The objective of the study was to investigate the effect of the leaf extract of Rosa canina L. against experimental diarrhea induced by castor oil in rodents. Materials and Methods: The methanol extract of Rosa canina L. (30 and 60 mg/kg body weight) was administered orally to two groups of mice (five animals per group) in order to evaluate the activity of the extract against the castor oil-induced diarrhea model in mice. Two other groups received normal saline and diphenoxylate (5 mg/kg) as positive control. The effect of the extract on intestinal transit and castor oil-induced intestinal fluid accumulation (enteropooling) was assessed. The effects of the extract on the isolated rabbit jejunum and on the isolated rat ileum were studied. Results: The preliminary phytochemical screening of the leaf extract of Rosa Canina L. revealed the presence of alkaloids, flavonoids, glycosides, saponins, and volatile oil. Intraperitoneal LD50 of the extract was found to be 455.19 ± 23 mg/kg in mice. The antidiarrheal effect of the methanolic extract exhibited a concentration-dependent inhibition of the spontaneous pendular movement of the isolated rabbit jejunum and inhibited acetylcholine-induced contraction of the rat ileum. A dose-dependent decrease in gastrointestinal transit was observed with extracts (30 and 60 mg/kg), which also protected mice against castor oil-induced diarrhea and castor oil-induced fluid accumulation, respectively. Conclusions: The presence of some of the phytochemicals in the leaf extract may be responsible for the observed effects, and also the basis for its use in traditional medicine as an antidiarrheal drug.


Samal H.B.,Guru Nanak Institute of Pharmacy | Dey S.,Guru Nanak Institute of Pharmacy | Kumar D.,Guru Nanak Institute of Pharmacy | Kumar D.S.,Guru Nanak Institute of Pharmacy | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

The present study involves design and characterization of floating microspheres with Nateglinide as model drug for prolongation of gastric residence time. Nateglinide Floating Microspheres were prepared by w/o/o emulsification solvent diffusion technique using rate controlling polymers ethyl cellulose and hydroxy propyl methyl cellulose. The shape and surface morphology of prepared microspheres were characterized by optical and scanning electron microscopy respectively. FTIR analyses the absences of drug-polymer interaction. In vitro drug release studies were performed and drug release kinetics was evaluated using the linear regression method. Effects of polymer concentration, solvent composition, particle size, drug entrapment efficiency and drug release were also observed. The prepared microspheres exhibited prolonged drug release (more than 12 h) and remained buoyant for > 24 h. The mean particle size increased and the drug release rate decreased at higher polymer concentration. In vitro studies demonstrated diffusion- controlled drug release from the microspheres.


Sandeep Kumar D.,Guru Nanak Institute of Pharmacy | Ashish Reddy B.,Guru Nanak Institute of Pharmacy | Tiwari A.K.,Guru Nanak Institute of Pharmacy | Swetha B.,Guru Nanak Institute of Pharmacy | Dey S.,Guru Nanak Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Recent advances in Novel Drug Delivery Systems (NDDS) aim to enhance safety and efficacy of drug molecule by formulating a convenient dosage form for administration and to achieve better patient compliance. One such approach is fast disintegrating/ dispersing tablet formulation. In the present work, fast disintegrating tablets of Felodipine were designed with a view to enhance patient compliance by direct compression method. In the direct compression method, crospovidone (2-10% w/w) was used as super-disintegrant along with microcrystalline cellulose (5-20%w/w) as disintegrant and directly compressible mannitol to enhance mouth feel. Estimation of Felodipine in the prepared tablet formulations was carried out by extracting the drug with methanol and measuring the absorbance at 360nm. The prepared formulations were further evaluated for hardness, friability, drug content uniformity, wetting time, water absorption ratio and in vitro dispersion time. Based on in vitro dispersion time (approximately 8-14 s), one promising formulation was tested for in vitro drug release pattern (in pH 6.8 phosphate buffer) and drug excipient interaction (IR spectroscopy). Among all the formulations, promising formulation (DCF3) the formulation prepared by direct compression method (containing 2% w/w crospovidone and 15% w/w microcrystalline cellulose) emerged as the overall best formulation.

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