Guru Nanak Institute of Pharmaceutical science and Technology

Pānihāti, India

Guru Nanak Institute of Pharmaceutical science and Technology

Pānihāti, India
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Kundusen S.,Jadavpur University | Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Bala A.,Jadavpur University | And 3 more authors.
Pharmacologyonline | Year: 2010

Citrus limetta and Citrus maxima belong to the genus Citrus (Rutaceae) and are rich in flavonoids, vitamin C, folate, dietary fibre, carotenoids etc. Different parts of both plants are responsible for the prevention of cancer, other degenerative diseases, anti-inflammatory activity and antidiabetic activity etc. The present study was aimed to evaluate the in vitro antioxidant activity of the methanol extracts of Citrus limetta fruit peel (MECL) and Citrus maxima (MECM) leaves in different models such as DPPH, superoxide radical and nitric oxide radical scavenging activity and inhibition of lipid peroxidation. The total phenolic compounds present in the extracts were estimated by Folin-Ciocalteu's reagent. Both the extracts have shown good free radical scavenging activity in a dose dependent manner. The IC50 values for MECM were found to be 5.5 μg/ml, 79.16 μg/ml, 174.97μg/ml and 111.5μg/ml respectively for DPPH, superoxide radical, nitric oxide radical inhibition and lipid peroxidation assays. The IC50 values for DPPH, superoxide radical, nitric oxide radical inhibition and lipid peroxidation assays of MECL were 7.36 μg/ml, 9.36 μg/ml, 136.45 μg/ml and 111 μg/ml respectively. Measurement of total phenolic compounds by Folin-Ciocalteu's reagent indicated that 1 mg extract contains 126.85 and 116.7 μg equivalent of pyrocatechol in MECL and MECM respectively. The present study reports significant in vitro antioxidant activity of the test plants.


Bhattacharya A.,Guru Nanak Institute of Pharmaceutical Science and Technology | Debnath S.K.,N R Vekaria Institute Of Pharmacy | Dey M.,N R Vekaria Institute Of Pharmacy | Kar D.,Guru Nanak Institute of Pharmaceutical Science and Technology
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The aim of present study was to assess the analgesic activity of seeds of Annona squamosa Linn. The mature plant seeds of Annona squamosa were collected and authenticated. Extraction of dried seeds was carried out with methanol by maceration. Painful reaction in the experimental animals can be produced by applying unpleasant stimuli such as thermal (radiant heat as source of pain) and physical pressure (tail compression). In both Eddy's hot plate method and tail-flick method the seed extract (250 mg/kg body weight) showed the significant analgesic activity and comparable with the standard drug Nimesulide (50mg/kg body weight).


Kundusen S.,Jadavpur University | Gupta M.,Jadavpur University | Mazumder U.K.,Jadavpur University | Haldar P.K.,Jadavpur University | And 6 more authors.
Diabetologia Croatica | Year: 2011

The aim of the present study was to evaluate antihyperglycemic activity of methanol extract of Citrus maxima leaf (MECM) in streptozotocin (STZ; 65 mg/kg b.w.) induced diabetic rats. Three days after STZ induction, diabetic rats received MECM orally at a dose of 200 and 400 mg kg -1 body weight daily for 15 days. Glibenclamide (0.5 mg kg -1 p.o.) was used as a reference drug. Blood glucose levels were measured on days 0, 4, 8 and 15. Serum biochemical parameters viz. SGOT, SGPT and ALP were estimated. The TBARS and GSH levels of the pancreas, kidney and liver were determined. MECM significantly (P<0.001) and in a dose dependent manner normalized blood glucose levels, serum biochemical parameters, decreased lipid peroxidation, and recovered GSH as compared to those of STZ control. The present study inferred that in STZ-induced diabetic Wistar rats, C. maxima leaf demonstrated a potential antihyperglycemic effect that might be attributed to its antioxidant property.


Dewanjee S.,Jadavpur University | Mandal S.C.,Jadavpur University | Sen Gupta A.,Guru Nanak Institute of Pharmaceutical Science and Technology
Journal of Pure and Applied Microbiology | Year: 2010

Diarrhoeal diseases are a leading cause of childhood morbidity and mortality in developing countries. Many new microbial causes of diarrhoea have been discovered during the past three decades. The present study was undertaken to evaluate the effect of methanol extract of the fruits of Anus semialata Murr for its antimicrobial potential against bacteria causing diarrhoea. Methanol extract was tested for its minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria causing diarrhoea. Further, the zones of inhibition produced by the crude extract against few sensitive strains was measured and compared with those of standard antibiotic ciprofloxacin. It is evident that the methanol extract is very active against the bacteria causing diarrhoea at low concentrations. The antibacterial efficacy of the fruit extract was found to decrease in the following order against different tested bacterial strains like, Escherichia coli, Vibrio cholerae, Shigella boydii, Shigella dysenteriae, Shigella sonnei and Salmonella typhimurium.


Chakraverty R.,Guru Nanak Institute of Pharmaceutical Science and Technology
International Journal of Pharmaceutical Sciences | Year: 2012

Oral controlled drug delivery systems represent the most popular form of sustained drug delivery systems for the obvious advantages of oral route of drug administration. Such systems release the drug with constant or variable release rates. The oral controlled release systems shows a typical pattern of drug release in which the drug concentration is maintained in the therapeutic window for a prolonged period of time (sustained release), thereby ensuring sustained therapeutic action. They are used as single dosage form. Present work involves preparation and evaluation of sustained release of microspheres of Norfloxacin employing sodium alginate as natural polymer. The technique employed for microencapsulation of the drug is ionotropic gelation.


Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Mazumder U.K.,Jadavpur University | Haldar P.K.,Jadavpur University | And 3 more authors.
Pharmacognosy Journal | Year: 2011

Introduction: Lagenaria siceraria is traditionally used in liver disorders and various free radicals induced diseases. The present study was carried out to evaluate the antioxidant and hepatoprotective activities of the aerial parts of L. siceraria methanol extract (MELS). Methods: DPPH, nitric oxide, superoxide, hydrogen peroxide, lipid peroxide free radical scavenging activity, reductive ability and total phenolic and flavonoid content of MELS were determined. Hepatoprotective activity of the extract was investigated against carbon tetrachloride induced hepatotoxicity in rats. Results: The results explored significant in vitro antioxidant activity of MELS. It also showed potent hepatoprotective activity in rat, which was evident from its significant effect on the levels of serum biomarker enzymes and total protein & bilirubin. Significant improvement of the endogenous antioxidant status by the treatment of MELS further reflects its hepatoprotective potential, which was finally substantiated by the histological studies of the liver tissues. Conclusion: The results reveal potent hepatoprotective activity of MELS which is probably attributed to its significant free radical scavenging activity and high polyphenolic and flavonoid contents.


Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Mazumder U.K.,Jadavpur University | Haldar P.K.,Jadavpur University | And 4 more authors.
International Journal of Research in Pharmaceutical Sciences | Year: 2011

One of the largest causes of mortality worldwide is cancer. Increasing interest and research on herbal medicine have revealed its importance in treating many diseases including cancer. The present study was carried out to evaluate the antitumor activity of methanol extract of Cucurbita maxima Duchesne aerial parts (MECM) on Ehrlich Ascites Carcinoma (EAC) model in mice. After inoculation of EAC cells into mice, treatment with MECM (200 and 400 mg/kg) and standard drug 5-Fluorouracil (20 mg/kg) were continued for 9 days. Evaluation of the effect of drug response was made by the study of tumor growth response including increase in life span, study of hemato-logical parameters, biochemical estimations, antioxidant assay of liver tissue and in vitro cytotoxicity. Experimen-tal results revealed that C.maxima possesses significant anticancer activity which may be due to its cytotoxicity and antioxidant properties. Further research is going on to find out the active principle(s) of MECM for its anti-cancer activity. ©JK Welfare & Pharmascope Foundation.


Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Kundu Sen S.,Jadavpur University | Bala A.,Jadavpur University | And 2 more authors.
International Journal of Cancer Research | Year: 2011

Cancer is one of the leading causes of mortality worldwide. Many of the cucurbitaceae plants possess antitumor activity. On the basis of traditional use, the present study was carried out to evaluate the anti-cancer activity of methanol extract of Lagenaria siceraria (Mol.) Standley [Cucurbitaceae] aerial parts (MELS) on Ehrlich's Ascites Carcinoma (EAC) model in mice. After inoculation of EAC cells into mice, treatment with MELS (200 and 400 mg kg-1) and standard drug, 5-Fluorouracil (20 mg kg-1) were continued for 9 days. Evaluation of the effect of drug response was made by the study of tumor growth response including increase in life span, study of hematological parameters, biochemical estimations and antioxidant assay of liver tissue. Experimental results revealed that L. siceraria possesses significant anticancer activity which may be due to its cytotoxicity and antioxidant properties. Further research is ongoing to find out the bioactive principle(s) of MELS for its anticancer activity. © 2011 Academic Journals Inc.


Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Mazumder U.K.,Jadavpur University | Haldar P.K.,Jadavpur University | And 2 more authors.
Diabetologia Croatica | Year: 2011

The present study was carried out to evaluate the antihyperglycemic activity of methanol extract of Lagenaria siceraria aerial parts (MELS) for its purported use in diabetes. Hyperglycemia was induced by streptozotocin (50 mg/kg, i.p.) in rats. Treatment was done by MELS at doses of 200 and 400 mg/kg, p.o. for 14 days. Glibenclamide (500 μg/kg) was used as a reference drug. Antihyperglycemic potential was assessed by fasting blood glucose (FBG) measurement (on days 0, 4, 8 and 15), biochemical tests (SGPT, SGOT, ALP, total cholesterol, triglycerides), antioxidant assay (lipid peroxide, catalase and glutathione) and histologic study of the liver, kidney and pancreas tissue. Significant reduction (P<0.001) in FBG levels was observed with treatment duration. Antioxidant and biochemical parameters were significantly improved by MELS and glibenclamide treatment. Histologic observations showed good correlations with the results obtained. The study explored the potent antihyperglycemic activity of MELS, which is probably attributable to its rich flavonoid content.


Saha P.,Jadavpur University | Saha P.,Guru Nanak Institute of Pharmaceutical Science and Technology | Bala A.,Jadavpur University | Kar B.,Jadavpur University | And 4 more authors.
Research Journal of Medicinal Plant | Year: 2011

The objective of the present study was to evaluate the antidiabetic activity of methanol extract of aerial parts of Cucurbita maxima (MECM) for its purported use in diabetes. Antidiabetic activity of MECM was evaluated in Wistar albino rats against streptozotocin (50 mg kg-1, i.p.,) at the doses of 200 and 400 mg kg-1, p.o. for 14 days. Glibenclamide (500 μg kg-1) was used as reference drug. Fasting blood glucose (FBG) levels were measured on day 0, 4, 8 and 15. Antioxidant activity of the extract was determined by measuring lipid peroxide, catalase and glutathione (reduced) levels of liver, kidney and pancreas. In addition, biochemical parameters (SGPT, SGOT, ALP) were estimated. Histological study of liver, kidney and pancreas was also performed. FBG levels were reduced in a treatment-duration dependant manner and 60.88, 67.79 and 64.30% reduction with respect to the initial levels were observed on 15th day with low and high dose of MECM and glibenclamide, respectively. The changes in antioxidant and biochemical parameters observed with diabetic control group were reversed towards normal levels with extract and standard treated groups, rlistological observations showed good correlations with other results. The results explored potent antidiabetic activity of the methanol extract of C. maxima aerial parts. © 2011 Academic Journals Inc.

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