Seyhan N.,University of Konya |
Gunal A.,Gulhane Military Academy |
Avunduk M.C.,University of Konya |
Savaci N.,University of Konya
Annals of Plastic Surgery | Year: 2015
Background Free fat grafts have an unpredictable survival rate that limits their successful use. To increase the viability of fat grafts, it is important to minimize the reabsorption rate. Objective Our aim was to investigate whether the combined use of platelet-rich plasma (PRP) and adipose derived stem cells (ADSCs) would contribute an improvement in lower resorption rates of fat grafts. Methods Inbred Fischer 344 rats were randomized into 4 groups (n = 10). Fat grafts were mixed with Dulbecco modified Eagle medium in group A, with PRP in group B, with ADSC in group C, and with PRP + ADSC in group D and were injected to the scalp. In vitro growth factor (vascular endothelial growth factor, transforming growth factor-β, and fibroblast growth factor) levels were compared using enzyme-linked immunoassay method. After 12 weeks weight, volume and histology of the transplants were evaluated. Results The mean weight and volume of the fat grafts were highest in group D. Histopathological investigations revealed that the number of viable adipocytes and blood vessels were highest in group D. The level of growth factors was significantly higher in stem cell plus PRP group. Conclusion Adipose-derived stem cells combined with PRP can enhance the survival of transplanted fat tissue. © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Atmanli A.,Turkish Land Forces NCO Vocational College |
Ileri E.,Gulhane Military Academy |
Yuksel B.,Balikesir University |
Yilmaz N.,New Mexico Institute of Mining and Technology
Applied Energy | Year: 2015
In this study, n-butanol (nB) was used as the common solvent and crude canola (Cn), soybean (Sb), sunflower (Sf), corn (Cr), olive (Ol), and hazelnut oil (Hn) as the vegetable oil components were used for making microemulsification of diesel fuel (D)-vegetable oil blends. The blend ratios of 70% vol. D, 20% vol. vegetable oil and 10% vol. nB were determined to increase concentration of biofuels in ternary blends. Six different ternary blends were prepared via the splash blending method. Engine performance tests of the ternary blends were carried out on a four-cylinder, four-cycle turbocharged direct-injection diesel engine at full load with various engine speeds. Test fuels were kept stationary at ambient temperature and the long-term stabilities were observed at 60days. There were not any phase separations in the ternary blends. Fuel properties of the test fuels were examined and determined to be in agreement with the fuel standards. According to engine performance test results of the ternary blends, brake torque, brake power, brake thermal efficiency (BTE), brake mean effective pressure (BMEP) and exhaust gas temperatures decreased while brake specific fuel consumption (BSFC) increased as compared to those of diesel fuel. In terms of basic exhaust gas emissions, ternary blends increased nitric oxide (NO) and carbon monoxide (CO) emissions while reducing hydrocarbon (HC) and carbon dioxide (CO2) emissions as compared to diesel. © 2015 Elsevier Ltd.
PubMed | Istanbul Science University, Marmara University and Gulhane Military Academy
Type: Journal Article | Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society | Year: 2016
The importance of physical activity in the management of renovascular diseases is well-known, but lacks evidence of underlying mechanisms. The purpose of the study was to elucidate the protective/therapeutic effects of regular exercise on experimental renovascular hypertension (RVH)-induced oxidative stress and cardiac dysfunction. Wistar albino rats underwent a RVH surgery (2K1C, Goldblatt). Three weeks later half of the rats started swimming exercise for 9 weeks (n = 15), while the sedentary RVH group (n = 15) had no exercise during that period. Sham-operated control rats (n = 10), had the similar surgical procedures but the left renal artery was left unclipped. Body weights were monitored, and blood pressures were measured weekly using tail-cuff. Echocardiographic evaluation was performed on the 3(rd) week and on the 12(th) week of the experiment before the rats were decapitated. Heart and thoracic aorta were removed and serum was collected, while aortic samples were put in a 10% formaldehyde solution for immunochemistry. Cardiac tissue samples obtained from each animal were used for the determination of tissue myeloperoxidase (MPO) and catalase (CAT) activities, malondialdehyde (MDA), and glutathione (GSH) levels. In the sedentary RVH group, aortic contractile response (contraction/relaxation in isolated organ bath), left ventricular diastolic and systolic dimensions, and immunohistochemical staining of aortic inducible nitric oxide synthase (iNOS) were increased, while ejection fraction and aortic endothelial nitric oxide synthase (eNOS) staining were decreased. RVH in the sedentary rats resulted in increased pro-inflammatory cytokines (TNF-, IL-2, IL-6), lipid peroxidation (malondialdehyde) and neutrophil infiltration (myeloperoxidase activity) along with reductions in antioxidant glutathione and catalase levels in the cardiac tissue. Exercise after RVH increased the immunohistochemical staining of aortic eNOS, decreased iNOS staining and reversed the alterations in echocardiographic and oxidative parameters. Regular exercise commenced after RVH surgery alleviated renovascular hypertension-induced oxidative injury, by modulating oxidant-antioxidant balance via the involvement of the endothelial NO system.
Atmanli A.,Turkish Land Forces NCO Vocational College |
Ileri E.,Gulhane Military Academy |
Yilmaz N.,New Mexico Institute of Mining and Technology
Energy | Year: 2016
The rule of thumb in literature is that 20% of biodiesel is the most acceptable blend ratio in alternative fuel blends. This work focuses on in-depth mathematical optimization analyses of ternary blends of diesel-butanol-vegetable oil (cotton oil), based on engine operating parameters using RSM (response surface methodology). It is critical to achieve the maximum power and torque for customers while keeping the emissions low enough due to government regulations and certifications. Thus, three optimization studies were conducted at 2200 rpm, which corresponds to the maximum brake torque, and engine emissions were fixed at a maximum possible value based on emission standards, for all three studies. In order to understand the impact of other engine parameters on the blend ratio, as well, various combinations of BTE (brake thermal efficiency), maximum brake power, maximum brake torque, BSFC (brake specific fuel consumption) and BMEP (brake mean effective pressure) were fixed, which correspond to Opt-(optimization 1) (BTE and exhaust emissions), optimization 2 (BTE, brake power and exhaust emissions), and optimization 3 (BTE, brake power, brake torque, BSFC, BMEP and exhaust emissions). Optimization studies used experimentally determined emissions and performance data of a diesel engine based on 7 different concentrations of diesel-butanol-cotton oil blends. Optimum values of the blends corresponding to the optimization studies were mathematically determined as Opt-(optimization 1) (61.7 vol.% diesel, 34.75 vol.% butanol, 3.55 vol.% cotton oil), Opt-(optimization 2) (64.5 vol.% diesel, 28.7 vol.% butanol, 6.8 vol.% cotton oil), and Opt-(optimization 3) (65.5 vol.% diesel, 23.1 vol.% butanol, 11.4 vol.% cotton oil). When compared to diesel, BSFCs of Opt-1, Opt-and Opt-blends at 2200 rpm increased 41.57, 33.87 and 24.53%, respectively. In terms of basic exhaust gas emissions, optimum fuel blends decreased NOx (oxides of nitrogen), CO (carbon monoxide) and HC (hydrocarbon) emissions as compared to diesel. © 2015 Elsevier Ltd.
Ozen S.,Hacettepe University |
Demirkaya E.,Gulhane Military Academy |
Duzova A.,Hacettepe University |
Erdogan O.,Sami Ulus Childrens Hospital |
And 8 more authors.
Annals of the Rheumatic Diseases | Year: 2014
Background Colchicine is the main treatment for familial Mediterranean fever (FMF). However, biological agents and other treatments are available for patients who are unable to receive optimal treatment. Objective To develop outcome criteria that define response to treatment. Methods Two rounds of Delphi exercise were followed by a consensus conference enabling the definition of the criteria to be employed. Data for patients with FMF responding and resistant to their treatment were obtained from the FMF Arthritis Vasculitis and Orphan disease Research in paediatric rheumatology (FAVOR) website. The suggested criteria were analysed and validated in this patient cohort. Sensitivity/specificity measures and the ability of the score to discriminate between patients with active and inactive disease via the best cut-off score were calculated by a receiver operating characteristic analysis. Results Compliance with the maximum dose of the drug was considered essential for evaluation of the patients. Seven criteria were suggested in the consensus conference. The performance of each criterion, in differentiating between resistant and responsive patients, was tested. The final set of criteria was defined as at least 50% improvement in five of six criteria, without worsening in any one defined response to treatment with a very high sensitivity and specificity. The items of this FMF50 included: 1. Percentage change in the frequency of attacks with the treatment. 2. Percentage change in the duration of attacks with the treatment. 3. Patients/parents' global assessment of disease severity (10 cm visual analogue scale (VAS)). 4. Physicians' global assessment of disease severity (10 cm VAS). 5. Percentage change in arthritis attacks with the treatment. 6. Percentage change in C-reactive protein, erythrocyte sedimentation rate or serum amyloid A level with the treatment. Conclusions The FMF50 produced is a user-friendly measurement tool to guide physicians and can be used in clinical trials.
Verim S.,Mevki Hospital |
Tasci I.,Gulhane Military Academy
Turk Kardiyoloji Dernegi Arsivi | Year: 2013
Systemic atherosclerosis is a condition which progresses with age, decreases quality of life, and life expectancy. Lower extremity peripheral arterial disease (PAD) is a common manifestation of systemic atherosclerosis in the elderly. These individuals have a 2 to 4 fold higher risk of coronary heart disease and stroke. In addition, systemic atherosclerosis causes overall functional disability including restricted lower extremity movements. When used alone for diagnostic purposes, claudication is an unreliable sign of PAD in all age groups especially the elderly. Moreover, claudication is difficult to define due to the advancing age and degenerative changes in lumbar and peripheral joints. Doppler ultrasonography (US) is an easily available and noninvasive means of arterial visualization in the lower extremities. In this review, supporting evidence for the use of Doppler US in the diagnosis of PAD will be discussed. Past and present recommendations regarding Doppler US in the current PAD guidelines will be overviewed. © 2013 Turkish Society of Cardiology.
Cekmez F.,Gulhane Military Academy |
Cetinkaya M.,Gulhane Military Academy |
Tayman C.,Gulhane Military Academy |
Canpolat F.E.,Gulhane Military Academy |
And 4 more authors.
Regulatory Peptides | Year: 2013
Background: Necrotizing enterocolitis (NEC) is one of the most common gastrointestinal emergencies in newborn infants but up to now there is no completely effective treatment for it. Objective: In order to show that a combination of melatonin and prostaglandins may be useful to save lives, we use newborn rat as a model of necrotizing enterocolitis to test the hypothesis of using the combination therapy might have more potential effect on mucosal cytoprotection and healing. Patients and methods: A total of 60 newborn pups from 5 time-mated Sprague-Dawley pregnant rats were divided equally into 5 groups as follows: NEC (subjected to NEC), NEC+Melatonin, NEC+Prostaglandin, NEC+Prostaglandin+Melatonin and control. These animals were fed with hyperosmolar formula 3 times daily and subjected to 100% CO2 inhalation for 10min, +4°C cold exposure for 5min, and 97% O2 for 5min twice daily to induce NEC. This procedure was applied to the pups for 3days. Results: The macroscopic scoring, intestinal injury scoring and apoptosis index scoring were all found to be significantly lower in NEC. +. Prostaglandin. +. Melatonin group compared with NEC group. Anti-oxidant enzyme activities were significantly higher, whereas lipid peroxidation was significantly lower in NEC. +. Prostaglandin. +. Melatonin group compared with NEC group. Conclusion: This combination therapy showed cytoprotective and healing effects on mucosa in the intestinal tissue of rat pups in necrotizing enterocolitis model. Therefore, this therapy might also show benefit in preterm infants with NEC. After confirmation of this data by other clinical and experimental studies, it may be a novel therapeutic option for the prevention of NEC in preterm infants. © 2013 Elsevier B.V.
Yakupoglu H.,Medicana International Hospital |
Onal M.B.,Medicana International Hospital |
Civelek E.,Medicana International Hospital |
Kircelli A.,Gulhane Military Academy |
Celasun B.,Medicana International Hospital
Neurologia i Neurochirurgia Polska | Year: 2010
Pituitary adenoma infarction associated with cardiac surgery occurs rarely but it is a serious, life-threatening acute clinical event. Several mechanisms have been proposed but no direct cause has been clearly identified. We report a case of pituitary apoplexy occurring in a 74-year-old patient 6 hours after cardiac surgery. The patient presented with confusion, unilateral ptosis and ophthalmoplegia. Neurological examination revealed right oculomotor nerve palsy and decreased level of consciousness. Magnetic resonance imaging showed a hemorrhagic and necrotic pituitary macroadenoma. After prompt endocrinological replacement therapy with hydrocortisone and levothyroxine, the confusion of the patient resolved. Removal of a non-functional macroadenoma with large necrotic areas resulted in full recovery. The physician should be aware of pituitary adenoma infarction after open cardiac surgery and should remember that it can be fatal or cause permanent neurological or endocrine damage without proper treatment. Surgical and endocrine treatment can be life-saving procedures.
Ileri E.,Gulhane Military Academy
Energy Conversion and Management | Year: 2016
Vegetable oil based microemulsion blends have received increased attention due to its free major drawback like chemicals, catalysts and process heat of transesterification compared to biodiesel. However, the lower cetane number (CN) of a vegetable oil based microemulsion blends prevents its using in diesel engines because of poorer self-ignitability. This study focused on the effect of 2-ethylhexyl nitrate (EHN) (cetane improver) addition on fuel properties, and engine performance and exhaust emission characteristics of diesel engine fueled with microemulsion blends. EHN was added at 500, 1000 and 2000 ppm concentration to diesel (D) (70 vol.%), sunflower oil (S) (20 vol.%) and n-butanol (B) (D70S20B10) or 1-pentanol (P) (10 vol.%) (D70S20P10) microemulsion blends to study its effect. Microemulsion blends with EHN were tested on a turbocharged direct injection (TDI) diesel engine at fixed engine speed (2200 rpm) and five different engine loads. The addition of EHN to microemulsion blends had positive effect on CN without causing any significant negative effect on other fuel properties of microemulsion blends. Engine performance test results showed that microemulsion blends with EHN had little effect on brake power and brake mean effective pressure (BMEP). Brake specific fuel consumption (BSFC) values reduced with increase of EHN concentration in the microemulsion blends in the range of 2.49-8.17%. Addition of EHN to microemulsion blends produced lower mean oxides of nitrogen (NOx) in the range of 0.26-5.26%. D70S20B10 with 2000 ppm EHN has the best effects on the reduction of NOx emissions. However, carbon monoxide (CO) emissions increased in the range of 7.16-23.46% with increased presence of EHN concentration in microemulsion blends. Different trend in hydrocarbon (HC) emissions for D70S20B10 and D70S20P10 with EHN were observed. © 2016 Elsevier Ltd. All rights reserved.
Hapa O.,Gulhane Military Academy |
Barber F.A.,Plano Orthopedic and Sports Medicine Center
Minerva Ortopedica e Traumatologica | Year: 2010
Both patellar malalignment and trauma can lead to instability, that is a common condition most often occurring in adolescent females. Instability can range from subluxations to frank dislocations and can result from abnormalities in the bone structure or soft tissue problems such as a torn medial patellofempral ligament or weak vastus medialis obliquus. Nonoperative treatment should include a course of bracing and physical therapy to strengthen the vastus medialis obliquus and the gluteal muscles. Various surgical treatment options exist but an isolated lateral release is not supported by the recent literature. A comprehensive treatment algorithm will be here discussed.