Gujarat Vidyapeeth

Ghandinagar, India

Gujarat Vidyapeeth

Ghandinagar, India
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Patel V.R.,Gujarat Vidyapeeth | Bhatt N.S.,Gujarat Vidyapeeth | ByBhatt H.,Hemchandracharya North Gujarat University
Chemical Engineering Journal | Year: 2013

Myceliophthora vellerea HQ871747 was isolated from decayed wood sample and was subjected to phylogenetic analysis by using basidiomycete-specific ITS primers. Preferable physicochemical parameters were identified to achieve maximum enzyme and decolorization efficiency. M. vellerea HQ871747 tolerated 20gL-1 NaCl concentration without decrease in average rate of decolorization and showed more than 50% decolorization up to 7th cycle of repetitive dye addition. It is also able to degrade mixture of various reactive, acidic, direct and disperse dyes. In immobilized packed-bed reactor 29-65mgL-1h-1 decolorization rate was achieved with 80% COD removal, 154 and 75UL-1MnP and laccase activity respectively. Purified MnP and laccase were monomeric protein with corresponding 37 and 25kDa size on the SDS-PAGE. In this study it is proposed that the asymmetric cleavage of RB220 and HPLC, NMR and GC-MS confirmed formation of 2-diazo benzene sulfonic acid, naphthalene-2-sulfonic acid and 4-(4-diazenyl-5-oxo-4, 5-dihydro-1H-pyrazol-1-yl) benzene sulfonic acid. Treated wastewater was found to stimulate the growth of selected soil bacteria as well as plant growth. © 2013 Elsevier B.V.


Dave A.M.,Gujarat Vidyapeeth | Mevada V.A.,Hemchandracharya North Gujarat University | Bhatt N.S.,Gujarat Vidyapeeth | Acharaya P.B.,Gujarat Vidyapeeth | Patel R.K.,Hemchandracharya North Gujarat University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Incorporating receptor flexibility is considered crucial for improvement of docking based virtual screening with an abundance of crystallographic structure are freely available, docking with multiple crystal structure is believed to be a practical approach to cope with protein flexibility. 2SRC structures were first compared in a single structure docking by predicting the binding mode and recovering known ligands. Combination of different protein structures were compared by recovery of known ligands and an optional ensemble of 2SRC structure were selected. The chosen structure was used in virtual screening of over 2700 diverse compounds for 2SRC inhibitors. Six novel drugs ranked at the top of the hits list were tested experimentally proved. Further study indicated that achieving a better enrichment and identifying more diverse compounds was more likely using multiple structures than using only a single structure ever when protein structure were randomly selected. Taking into account conformational energy difference did not help to improve enrichment in the top ranked list.


Adhyaru D.N.,Gujarat Vidyapeeth | Bhatt N.S.,Gujarat Vidyapeeth | Modi H.A.,Gujarat University | Divecha J.,Sardar Patel University
Biocatalysis and Agricultural Biotechnology | Year: 2016

The present study focused on xylanase production from Aspergillus tubingensis FDHN1 under solid state fermentation at different flask levels and its high yield recovery process. The maximum xylanase production, 4061.32 U/g, was achieved in different sized flasks. The study further aimed at higher yield recovery in a single step from fermented sorghum straw through statistical methodology. For optimizing recovery conditions with respect to extractant volume (8-16 mL/g), extraction time (30-150 min), extraction speed (50-250 rpm) and extraction temperature (30-50 °C), central composite design (CCD) in 27 runs was employed. Maximum xylanase yield under validated recovery conditions was 5177.23 U/g with 90% recovery efficiency. Next, the crude xylanase, commercial cellulase and combination of both were subjected for saccharification of various untreated and pretreated agro-residues. The studied enzymes yielded much higher sugar content in combination, rather than their utilization as alone. Overall, xylanase from Aspergillus tubingensis FDHN1 can be produced and recovered at high level under solid state fermentation, exhibiting potential applications. © 2016 Elsevier Ltd.


Nathani N.M.,Anand Agricultural University | Nathani N.M.,Saurashtra University | Duggirala S.M.,Gujarat Vidyapeeth | M. C.S.,Anand Agricultural University | And 2 more authors.
Genomics Data | Year: 2015

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number JQHY00000000. The genome comprises of a 3.62-Mb draft genome with a G. +. C content of 28.10%, which encodes a total of 3126 proteins. Functional analysis provides information about the microbe's role in maintaining host homeostasis and its fiber degradation potential. © 2015 .


PubMed | Gujarat Vidyapeeth, Anand Agricultural University and Saurashtra University
Type: | Journal: Genomics data | Year: 2015

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number JQHY00000000. The genome comprises of a 3.62-Mb draft genome with a G+C content of 28.10%, which encodes a total of 3126 proteins. Functional analysis provides information about the microbes role in maintaining host homeostasis and its fiber degradation potential.


Adhyaru D.N.,Gujarat Vidyapeeth | Bhatt N.S.,Gujarat Vidyapeeth | Modi H.A.,Gujarat University
Biocatalysis and Agricultural Biotechnology | Year: 2014

A newly isolated Bacillus altitudinis DHN8 was assessed for xylanase production by utilizing sorghum straw. The highest xylanase production was recorded at sorghum straw 3% w/v, inoculum size 1% v/v, inoculum age 18h, incubation time 42h, pH 7.0, temperature 35°C and agitation speed 250rpm. Moreover, xylose 0.5%, gelatine 0.5% and KNO3 0.3% (w/v) further enhanced the production. The detailed optimization study resulted in a 3.74-fold increase in xylanase production as compared to that of the initial conditions. The partially purified xylanase showed ~70% pH stability after 18h at pH range of 6-10. Thermostability study revealed more than 60% xylanase activity at temperature range 45-65°C after 60min. The presence of metal ions (10mM CaCl2, MnCl2 and FeCl3) and solvents (10% v/v isopropanol, methanol, ethanol and acetone) were increased xylanase activities remarkably. During saccharification study, 3% alkaline hydrogen peroxide treatment was found to be beneficial for the maximum enzymatic hydrolysis of sorghum straw (34.94mg/g reducing sugar) after 36h. As such, this xylanase could be considered as a cellulase-free, thermo-alkali-solvent stable biocatalyst being important tool for many biotechnological industries.To the best of our knowledge, this is the first report on the production of xylanase by this Bacillus species. © 2013 Elsevier Ltd.


Bhatt N.S.,Gujarat Vidyapeeth | Patel D.S.,Gujarat Vidyapeeth
Indian Journal of Environmental Protection | Year: 2012

Synthetic dyes are extensively used in textile dyeing, paper, printing, colour, photography, pharmaceutics, cosmetics and other industries. Among these, azo dyes represent the largest and most versatile class of synthetic dyes. Approximately 10-15% of the dyes are released into the environment during manufacture and usage. Traditional methods of treatment are found to be expensive and have operational problems. In the present studies bacterial cultures were isolated from Environmental Treatment Plant, Naroda, G.I.D.C, Ahmedabad, Gujarat were subjected for acclimatization to Cl. reactive blue 250 (RB 250), an azo dye in the Bushneil and Haas Broth (BHB). The most promising bacterial isolate was used for further dye degradation studies. The strain showed complete decolourization of the selected dye (RB 250-100 mg/L) within 8 hr in static anoxic condition. The optimum pH, temperature, inoculum size and carbon and nitrogen sources for the decolourization was 7.0, 37°C, 4.0% and glucose (0.2%) and nitrogen (0.5%), respectively. The biodegradation was monitored by UV-Vis, TLC-HPTLC and FTIR analysis. Down flow fixed film batch bioreactor was studied, gave good response upto 200 mg/L RB 250 dye concentration. The results suggest that the isolated organism PSNB6 as a useful tool to treat wastewater containing reactive dyes. © 2012 - Kalpana Corporation.


Najrana K.,Gujarat Vidyapeeth | Prateek S.,Gujarat Vidyapeeth | Mayur S.,Gujarat Vidyapeeth
Pollution Research | Year: 2015

Today many farmers in India and other countries have shifted from inorganic to organic farming. Present study compares soil properties in different farms managed for a long time organically and inorganically. Farm soils were collected at two stages of Coriandrum sativum crop growth, i.e. pre-harvest and post-harvest. Physico-chemical properties, microbial count and enzyme activities were found out in these soil samples. All parameters were analyzed in duplicate to minimize the experimental error and average values are presented here. Results show that organically managed soil has higher microbial counts and enzyme activities compared to inorganically managed soil. Further, inorganic soil contains higher nitrogen, phosphorus and potassium content than that of organic soil. Values for all the parameters, except pH, decreased at post-harvest stage compared to pre-harvest. Copyright © EM International.


Shah M.C.,Gujarat Vidyapeeth | Patel H.P.,Gujarat Vidyapeeth | Shilpkar P.G.,Biogas Research and Extension Center
Pollution Research | Year: 2015

Now a days many synthetic neutralization indicators are available and their number are continuously increasing. Industrial synthesis of indicators causes environmental pollution directly as well as indirectly. The present study deals with small effort to give alternate of synthetic neutralization indicators through principles of green chemistry. Lavender to pink coloured Argyreia nervosa flowers are available during July-December and March-April. The water extract 10% w/v shows different colours at different pH: Pink (0 < pH < 2.87); Light pink (2.87 ≤ pH < 3.09); Coolourless (3.09 ≤ pH < 6.00%) Green (6.9 ≤ pH) in pH metric titration of 0.1M HC1 → 0.1 M NaOH. Indicator properties for this extract may be due to change in structure of anthocyanine derivatives, present in flower with pH change. Water extract of Argyreia nervosa flowers is more suitable as an acid-base indicator in HCl → NaOH. It is not suitable for CH3COOH → NaOH and NH4OH → HCl titrations. Copyright © EM International.


The objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. The 16S rRNA gene analysis of developed halophilic consortium VN.1 showed that the bacterial consortium contained six bacterial strains, which were identified as Pseudomonas fluorescens HM480360, Enterobacter aerogenes HM480361, Shewanella sp. HM589853, Arthrobacter nicotianae HM480363, Bacillus beijingensis HM480362 and Pseudomonas aeruginosa JQ659549. Halophilic consortium VN.1 was able to decolorize up to 2,500 mg/L RB220 with >85% chemical oxygen demand (COD) reduction under static condition at 30°C and pH 8.0 in the presence of 7% NaCl. VN.1 also exhibited more than 85% COD reduction with >25 mg/(L h) rate of decolorization in the case of different reactive dye mixtures. We propose the symmetric cleavage of RB220 using Fourier transform infrared, high-performance liquid chromatography (HPLC), nuclear magnetic resonance and gas chromatography-mass spectrometry analysis, and confirmed the formation of sodium-4-aminobenzenesulfonate, sodium-6-aminonepthalenesulfonate, and sodiumbenzene/nepthalenesulfonate. Toxicity studies confirm that the biodegraded products of RB220 effluent stimulate the growth of plants as well as the bacterial community responsible for soil fertility. © IWA Publishing 2015.

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