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Nathani N.M.,Anand Agricultural University | Nathani N.M.,Saurashtra University | Duggirala S.M.,Gujarat Vidyapeeth | M. C.S.,Anand Agricultural University | And 2 more authors.
Genomics Data | Year: 2015

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number JQHY00000000. The genome comprises of a 3.62-Mb draft genome with a G. +. C content of 28.10%, which encodes a total of 3126 proteins. Functional analysis provides information about the microbe's role in maintaining host homeostasis and its fiber degradation potential. © 2015 . Source


Patel V.R.,Gujarat Vidyapeeth | Bhatt N.S.,Gujarat Vidyapeeth | ByBhatt H.,Hemchandracharya North Gujarat University
Chemical Engineering Journal | Year: 2013

Myceliophthora vellerea HQ871747 was isolated from decayed wood sample and was subjected to phylogenetic analysis by using basidiomycete-specific ITS primers. Preferable physicochemical parameters were identified to achieve maximum enzyme and decolorization efficiency. M. vellerea HQ871747 tolerated 20gL-1 NaCl concentration without decrease in average rate of decolorization and showed more than 50% decolorization up to 7th cycle of repetitive dye addition. It is also able to degrade mixture of various reactive, acidic, direct and disperse dyes. In immobilized packed-bed reactor 29-65mgL-1h-1 decolorization rate was achieved with 80% COD removal, 154 and 75UL-1MnP and laccase activity respectively. Purified MnP and laccase were monomeric protein with corresponding 37 and 25kDa size on the SDS-PAGE. In this study it is proposed that the asymmetric cleavage of RB220 and HPLC, NMR and GC-MS confirmed formation of 2-diazo benzene sulfonic acid, naphthalene-2-sulfonic acid and 4-(4-diazenyl-5-oxo-4, 5-dihydro-1H-pyrazol-1-yl) benzene sulfonic acid. Treated wastewater was found to stimulate the growth of selected soil bacteria as well as plant growth. © 2013 Elsevier B.V. Source


Shah M.C.,Gujarat Vidyapeeth | Patel H.P.,Gujarat Vidyapeeth | Shilpkar P.G.,Biogas Research and Extension Center
Pollution Research | Year: 2015

Now a days many synthetic neutralization indicators are available and their number are continuously increasing. Industrial synthesis of indicators causes environmental pollution directly as well as indirectly. The present study deals with small effort to give alternate of synthetic neutralization indicators through principles of green chemistry. Lavender to pink coloured Argyreia nervosa flowers are available during July-December and March-April. The water extract 10% w/v shows different colours at different pH: Pink (0 < pH < 2.87); Light pink (2.87 ≤ pH < 3.09); Coolourless (3.09 ≤ pH < 6.00%) Green (6.9 ≤ pH) in pH metric titration of 0.1M HC1 → 0.1 M NaOH. Indicator properties for this extract may be due to change in structure of anthocyanine derivatives, present in flower with pH change. Water extract of Argyreia nervosa flowers is more suitable as an acid-base indicator in HCl → NaOH. It is not suitable for CH3COOH → NaOH and NH4OH → HCl titrations. Copyright © EM International. Source


Khan R.,Gujarat Vidyapeeth | Khan Z.,Sardar Patel University | Nikhil B.,Gujarat Vidyapeeth | Jyoti D.,Sardar Patel University | Datta M.,Sardar Patel University
Bioremediation Journal | Year: 2014

Soil samples isolated from dye-contaminated sites were exploited for isolation of dye decolorizing microorganisms. A novel bacterial mixture, RkNb1, was selected based on its efficiency, showing maximum and faster decolorization of textile dyes. Seven bacterial strains were isolated and identified from the bacterial mixture as Ochrobactrum intermedium (HM480365), Ochrobactrum intermedium strain M16-10-4 (HM030758), Enterococcus faecalis (HM480367), Arthrobacter crystallopoietes (HM480368), Kocuria flavus (HM480369), Bacillus beijingensis (HM480370), and Citrobacter freundii (HM480371) by 16S rRNA gene sequence analysis. This bacterial mixture showed 98.17% decolorization of Reactive Violet 5 (400 mg L-1) within 8 h. The culture exhibited good decolorization ability at pH 8 and at a temperature of 37°C. Malt extract and peptone was found to enhance the decolorization rate of Reactive Violet 5. Plackett-Burman experimental design was used for elucidation of medium components affecting Reactive Violet 5 decolorization. Dye degradation products obtained during the course of decolorization were analyzed by high-performance thin-layer chromatography (HPTLC), Fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR). The potential of this bacterial mixture to decolorize Reactive Violet 5 dye from manufacturing industry effluent is to be carried out using appropriate bioreactors. © 2014 Taylor and Francis Group, LLC. Source


The objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. The 16S rRNA gene analysis of developed halophilic consortium VN.1 showed that the bacterial consortium contained six bacterial strains, which were identified as Pseudomonas fluorescens HM480360, Enterobacter aerogenes HM480361, Shewanella sp. HM589853, Arthrobacter nicotianae HM480363, Bacillus beijingensis HM480362 and Pseudomonas aeruginosa JQ659549. Halophilic consortium VN.1 was able to decolorize up to 2,500 mg/L RB220 with >85% chemical oxygen demand (COD) reduction under static condition at 30°C and pH 8.0 in the presence of 7% NaCl. VN.1 also exhibited more than 85% COD reduction with >25 mg/(L h) rate of decolorization in the case of different reactive dye mixtures. We propose the symmetric cleavage of RB220 using Fourier transform infrared, high-performance liquid chromatography (HPLC), nuclear magnetic resonance and gas chromatography-mass spectrometry analysis, and confirmed the formation of sodium-4-aminobenzenesulfonate, sodium-6-aminonepthalenesulfonate, and sodiumbenzene/nepthalenesulfonate. Toxicity studies confirm that the biodegraded products of RB220 effluent stimulate the growth of plants as well as the bacterial community responsible for soil fertility. © IWA Publishing 2015. Source

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