Guizhou Tobacco Science Research Institute

Guiyang, China

Guizhou Tobacco Science Research Institute

Guiyang, China
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Zhao J.,Guizhou Tobacco Science Research Institute | Xie S.,Guizhou Tobacco Science Research Institute | Wang Y.,Guizhou Tobacco Science Research Institute | Pu W.,China Tobacco Hunan Industrial Co. | And 4 more authors.
Acta Tabacaria Sinica | Year: 2013

GGE biplot was used to analyze tobacco leaf characteristics of 11 flue-cured tobacco cultivars or lines from 7 growing areas. Characteristics and their correlation with sensory quality, chemical components and appearance quality were evaluated. Results showed that characteristics were significantly correlated with the ratio of reducing sugar to total sugar (-0.873**), the ratio of total nitrogen to nicotine (-0.822*), and nicotine (0.704*). Tobacco leaves showed different characteristics from different planting areas. All these were useful for determining best suitable growing areas as well as variety development.


Shao X.,Hohai University | Wang Y.,Hohai University | Bi L.,Hohai University | Dai L.,Hohai University | And 3 more authors.
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2010

For promoting the insufficiency in evaluating soil water availability (SWA) through soil water characteristic curve, soil water characteristic curves were partitioned by means of optimum partitioning clustering theory and clustering centre values were selected as evaluation indices. A projection pursuit evaluation model was constructed to evaluate soil water validity of 33 soil samples taken from 11 main tobacco-growing areas in Guizhou Province, China. The results were compared with those of conventional method by using soil physical properties as evaluation indices. The results showed that the selected indices could reflect soil water regime of tobacco field, and the results of the evaluation according to the indices were more comprehensive than those from conventional method.


Meng J.,Guizhou Tobacco Science Research Institute | Zhang C.,Guizhou University | Chen X.,Guizhou Tobacco Science Research Institute | Cao Y.,Guizhou Tobacco Science Research Institute | Shang S.,Guizhou Tobacco Science Research Institute
Pesticide Biochemistry and Physiology | Year: 2014

Myzus persicae, a serious economic agricultural pest, has developed resistance to imidacloprid (IMI), which was widely used to control this aphid worldwide. To gain a better understanding of the mechanisms of IMI resistance in M. persicae, we carried out a comparative proteomic analysis. Total proteins of the IMI-susceptible and resistant strains were extracted and separated by two-dimensional gel electrophoresis. More than 1300 protein spots were reproducibly detected, including 14 that were more abundant and 14 less abundant. Mass spectrometry analysis and database searching helped us to identify 25 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, transport processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in IMI-susceptible and resistant M. Persicae, and gives new insights into the mechanisms of IMI resistance in M. persicae. © 2014 Elsevier Inc.


PubMed | Guizhou University and Guizhou Tobacco Science Research Institute
Type: | Journal: Pesticide biochemistry and physiology | Year: 2014

Myzus persicae, a serious economic agricultural pest, has developed resistance to imidacloprid (IMI), which was widely used to control this aphid worldwide. To gain a better understanding of the mechanisms of IMI resistance in M. persicae, we carried out a comparative proteomic analysis. Total proteins of the IMI-susceptible and resistant strains were extracted and separated by two-dimensional gel electrophoresis. More than 1300 protein spots were reproducibly detected, including 14 that were more abundant and 14 less abundant. Mass spectrometry analysis and database searching helped us to identify 25 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, transport processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in IMI-susceptible and resistant M. Persicae, and gives new insights into the mechanisms of IMI resistance in M. persicae.


Zhang Y.,Chinese Academy of Agricultural Sciences | Liu Q.,Chinese Academy of Agricultural Sciences | Li Z.,Chinese Academy of Agricultural Sciences | Jiang W.,Zunyi Branch of Guizhou Provincial Tobacco Company | And 7 more authors.
Tobacco Science and Technology | Year: 2015

Topography influences the density of soil sampling points for precise fertilization. In the case of high phosphorus and potassium fertilization for flue-cured tobacco, the soils in areas of plain, broad valley, narrow valley and gentle slope were sampled by 50 m×50 m grids and the available nutrients in soil samples were tested. The results showed that by Cochran formula, individual sample for plain, valley and gentle slope represented an area of 1.92, 1.01-1.56 and 0.73 ha, respectively at a confidence level of 90% and a relative error of 15%; by geostatistical method, the maximum sampling space for precise nutrient management were 234.0, 134.4-177.5 and 58.2 m for plain, valley and gentle slope, respectively. It indicated that sampling density should be increased gradually from flat to slope. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Zhang J.,Guizhou Tobacco Science Research Institute | Ge Y.,Guizhou Tobacco Science Research Institute | Xiang Z.,Guizhou Tobacco Science Research Institute | Zhou S.,Guizhou Tobacco Science Research Institute | And 2 more authors.
Tobacco Science and Technology | Year: 2015

In order to determine the acid detergent fiber (ADF) and acid detergent lignin (ADL) in tobacco simultaneously, and taking account of that the ADF value inclusive of ash could be obtained during the ADL analyzing process of Van Soest method, the influences of ash correction on the measurement of ADF in tobacco samples of different types were investigated and the relationship between the residual silicate contents in ADF and ADL was studied. The results showed that: 1) The silicate content in ADF extremely significantly positively correlated to the ash content in samples and the silicate content in ADL. 2) For tobacco samples of high ash content, excluding flue-cured tobacco, the residual silicate would create bigger positive deviations in ADF determination. However, a relative deviation of less than 5% could be achieved via correcting the original measurement in said ADL analyzing. The analysis efficiency was improved remarkably by achieving the simultaneous determination of ADF and ADL dispensing with ADF ashing. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Qiu X.,Guizhou Tobacco Science Research Institute | Gao W.,Guizhou Tobacco Science Research Institute | Chen W.,Guizhou Tobacco Science Research Institute | Liang G.,Guizhou Tobacco Science Research Institute | Tu Y.,Guizhou Tobacco Science Research Institute
Tobacco Science and Technology | Year: 2015

In order to investigate the effects of soil temperature and humidity and ambient air temperature on the early stage growth and development of tobacco plants by well-cellar transplanting, the field experiments on transplanting means were conducted at Longgang base of Guizhou Tobacco Science Research Institute. The results showed that well-cellar transplanting aided by film mulching could effectively raise the soil and air temperatures in fields, narrow the variation ranges of soil temperature and humidity, and promote the early stage growth and development of tobacco plants. The tobacco plants showed the best agronomic traits, and highest biomass and root vigor 35 days after transplanting. The results of grey incidence analysis indicated that for well-cellar transplanting aided by film mulching, the effect of soil temperature on root development was greater than that of soil humidity, the effects of soil temperature at different soil depths differed, and the effect of soil temperature at 15 cm depth was greater. In contrast, root vigor was affected more obviously by soil humidity than did soil temperature, and the effect of soil humidity at 5 cm depth was greater. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Wei K.S.,Guizhou Tobacco Science Research Institute | Yang W.L.,Zhejiang University | Jilani G.,Pmas Arid Agriculture University | Zhou W.J.,Zhejiang University | And 4 more authors.
Journal of Animal and Plant Sciences | Year: 2012

This study investigated the influence of high temperature on the expression patterns of multiple DBE isoform genes (PUL, ISA1, ISA2 and ISA3) at RNA transcriptional level in developing endosperm, as well as its relation to the varying total activity of DBE and starch fine structure. Two rice genotypes with different amylose content and palatability were used for research under two temperature treatments (32°C and 22°C for the mean daily temperature, respectively) at filling stage. The temperature treatments were laid out in a completely randomized design. The data were analyzed using SPSS into one-way ANOVA, and means separation was carried out with the LSD method The result showed that high temperature resulted in the decrease of DP5-9 and DP 15-22, and increase of DP 10-13 and DP>42 of amylopectin in rice endosperm. For enzyme activity, PUL and ISA showed higher activities at initial stage, but substantially lower at later filling stage relative to low temperature. For different DBE isoform genes, ISA1 and ISA2 presented up-regulation pattern at high temperature over the whole sampling stage, while ISA3 and PUL showed the opposite case at the later filling stage in comparison of their corresponding low temperature. The DBEs isozyme activities and their transcriptional expression indicated that ISA2 was one of the dominant DBE isoforms highly expressed at later filling stage. The contribution of ISA3 expression to total ISA activity was mostly at earlier filling stage when rice plants were subjected to high temperature. Further investigations are required to elucidate the coordinated action of multiple key enzymes in starch synthesis at high temperature and their relations to amylopectin fine structure and rice quality.


Zhu H.,Anhui University of Science and Technology | Feng Y.,Guizhou Tobacco Science Research Institute | Yang J.,Anhui University of Science and Technology | Pan W.,Guizhou Tobacco Science Research Institute | And 4 more authors.
Journal of Separation Science | Year: 2013

Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic-lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid-solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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