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Zhang Y.,Chinese Academy of Agricultural Sciences | Liu Q.,Chinese Academy of Agricultural Sciences | Li Z.,Chinese Academy of Agricultural Sciences | Jiang W.,Zunyi Branch of Guizhou Provincial Tobacco Company | And 7 more authors.
Tobacco Science and Technology | Year: 2015

Topography influences the density of soil sampling points for precise fertilization. In the case of high phosphorus and potassium fertilization for flue-cured tobacco, the soils in areas of plain, broad valley, narrow valley and gentle slope were sampled by 50 m×50 m grids and the available nutrients in soil samples were tested. The results showed that by Cochran formula, individual sample for plain, valley and gentle slope represented an area of 1.92, 1.01-1.56 and 0.73 ha, respectively at a confidence level of 90% and a relative error of 15%; by geostatistical method, the maximum sampling space for precise nutrient management were 234.0, 134.4-177.5 and 58.2 m for plain, valley and gentle slope, respectively. It indicated that sampling density should be increased gradually from flat to slope. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

Shao X.,Hohai University | Wang Y.,Hohai University | Bi L.,Hohai University | Dai L.,Hohai University | And 3 more authors.
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2010

For promoting the insufficiency in evaluating soil water availability (SWA) through soil water characteristic curve, soil water characteristic curves were partitioned by means of optimum partitioning clustering theory and clustering centre values were selected as evaluation indices. A projection pursuit evaluation model was constructed to evaluate soil water validity of 33 soil samples taken from 11 main tobacco-growing areas in Guizhou Province, China. The results were compared with those of conventional method by using soil physical properties as evaluation indices. The results showed that the selected indices could reflect soil water regime of tobacco field, and the results of the evaluation according to the indices were more comprehensive than those from conventional method.

Zhao J.,Guizhou Tobacco Science Research Institute | Xie S.,Guizhou Tobacco Science Research Institute | Wang Y.,Guizhou Tobacco Science Research Institute | Pu W.,China Tobacco Hunan Industrial Co. | And 4 more authors.
Acta Tabacaria Sinica | Year: 2013

GGE biplot was used to analyze tobacco leaf characteristics of 11 flue-cured tobacco cultivars or lines from 7 growing areas. Characteristics and their correlation with sensory quality, chemical components and appearance quality were evaluated. Results showed that characteristics were significantly correlated with the ratio of reducing sugar to total sugar (-0.873**), the ratio of total nitrogen to nicotine (-0.822*), and nicotine (0.704*). Tobacco leaves showed different characteristics from different planting areas. All these were useful for determining best suitable growing areas as well as variety development.

Meng J.,Guizhou Tobacco Science Research Institute | Zhang C.,Guizhou University | Chen X.,Guizhou Tobacco Science Research Institute | Cao Y.,Guizhou Tobacco Science Research Institute | Shang S.,Guizhou Tobacco Science Research Institute
Pesticide Biochemistry and Physiology | Year: 2014

Myzus persicae, a serious economic agricultural pest, has developed resistance to imidacloprid (IMI), which was widely used to control this aphid worldwide. To gain a better understanding of the mechanisms of IMI resistance in M. persicae, we carried out a comparative proteomic analysis. Total proteins of the IMI-susceptible and resistant strains were extracted and separated by two-dimensional gel electrophoresis. More than 1300 protein spots were reproducibly detected, including 14 that were more abundant and 14 less abundant. Mass spectrometry analysis and database searching helped us to identify 25 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, transport processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in IMI-susceptible and resistant M. Persicae, and gives new insights into the mechanisms of IMI resistance in M. persicae. © 2014 Elsevier Inc.

Zhu H.,Anhui University of Science and Technology | Feng Y.,Guizhou Tobacco Science Research Institute | Yang J.,Anhui University of Science and Technology | Pan W.,Guizhou Tobacco Science Research Institute | And 4 more authors.
Journal of Separation Science | Year: 2013

Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic-lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid-solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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