Guizhou Tobacco Research Institute

Guiyang, China

Guizhou Tobacco Research Institute

Guiyang, China
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Ma Y.,Tongren Tobacco Company | Luo H.,Tongren Tobacco Company | Song J.,Tongren Tobacco Company | Mo J.,Tongren Tobacco Company | And 4 more authors.
Acta Tabacaria Sinica | Year: 2013

Relationships between humus chemical composition and soil factors in typical tobacco-growing regions were analyzed. Results showed that: (1) carbon content (TC) of soil humus ranged from10 g/kg to 20 g/kg, with average of 15.25 g/kg. Proportions of humic acid (HA), fulvic acid (FA) and humin (HM) in humus carbon content were distributed in 11.05%-32.15%, 12.00%-36.28% and 38.43%-68.23%, respectively. Most of HA/FA ratios ranged from 0.7 to 1.3; (2) TC in tobacco growing regions in southeast China was significantly higher than in other regions, with Huanghuai region was the lowest. The HA/FA ratio of tobacco growing regions in southeast China was significantly higher than in others, with an average value of 1.14, while in other regions it was less than 1; (3) Carbon levels of TC and humus composition in paddy soil were the highest followed by purple, red, and brown soils. The humification degree of paddy soil was also much higher than others; (4) soil pH values were significantly negatively correlated with humus composition. Alkali-hydrolyzable nitrogen content increased with decreasing soil FA/TC, or increasing HA/FA.


Gu W.,Yunnan University | Yang J.,Yunnan University | Lou Z.,Tsinghua University | Liang L.,Yunnan University | And 8 more authors.
PLoS ONE | Year: 2011

Microbial ferulic acid decarboxylase (FADase) catalyzes the transformation of ferulic acid to 4-hydroxy-3-methoxystyrene (4- vinylguaiacol) via non-oxidative decarboxylation. Here we report the crystal structures of the Enterobacter sp. Px6-4 FADase and the enzyme in complex with substrate analogues. Our analyses revealed that FADase possessed a half-opened bottom β-barrel with the catalytic pocket located between the middle of the core β-barrel and the helical bottom. Its structure shared a high degree of similarity with members of the phenolic acid decarboxylase (PAD) superfamily. Structural analysis revealed that FADase catalyzed reactions by an "open-closed" mechanism involving a pocket of 8×8×15 Å dimension on the surface of the enzyme. The active pocket could directly contact the solvent and allow the substrate to enter when induced by substrate analogues. Site-directed mutagenesis showed that the E134A mutation decreased the enzyme activity by more than 60%, and Y21A and Y27A mutations abolished the enzyme activity completely. The combined structural and mutagenesis results suggest that during decarboxylation of ferulic acid by FADase, Trp25 and Tyr27 are required for the entering and proper orientation of the substrate while Glu134 and Asn23 participate in proton transfer. © 2011 Gu et al.


Zou Y.,Guizhou Tobacco Research Institute | Shi J.,Guizhou Tobacco Research Institute | Jiang W.,Guizhou Zunyi Tobacco Company | Meng L.,Guizhou Tobacco Research Institute | And 2 more authors.
Acta Tabacaria Sinica | Year: 2015

Effects of six different proportions of red and blue light of LED (light emitting diode) on seedling quality in multilayer system were studied. Two out of six proportions were selected to determine their impact on tobacco plant growth and tobacco leaf yield and quality. Results showed that light intensity had an enhanced trend with increase of proportion of blue light. The seedling rate under four proportions of red and blue light (1:1, 1:2, 1:3 and 1:4) was significantly higher than that under natural light. Dry matter accumulation was high, but seedling quality was similar to natural light. Chlorophyll content under LED light was significantly higher than that under natural light, but root activity under LED light was significantly lower. In addition, root activity under red and blue light with proportions of 1:1 and 1:4 was significantly stronger than that with other proportions. Tobacco plants with LED light treatment grew slow in root extending stage, and grew faster afterwards, and showed no significant difference with those under natural light treatment at maturing stage. The economic characters and chemical compositions of tobacco leaves under LED light treatments showed no significant difference with those under natural light. It is concluded that red and blue light with proportions of 1:1 and 1:4 could increase seedling rate and chlorophyll content of seedling leaves, and promote accumulation of dry matter. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


PubMed | CAS Kunming Institute of Zoology, Guizhou Tobacco Research Institute and Kunming University of Science and Technology
Type: | Journal: Journal of biological research (Thessalonike, Greece) | Year: 2016

Nicotiana belongs to the Solanaceae family that includes important crops such as tomato, potato, eggplant, and pepper. Nicotiana species are of worldwide economic importance and are important model plants for scientific research. Here we present the comparative analysis of the transcriptomes of six wild diploid Nicotiana species. Wild relatives provide an excellent study system for the analysis of the genetic basis for various traits, especially disease resistance.Whole transcriptome sequencing (RNA-seq) was performed for leaves of six diploid Nicotiana species, i.e. Nicotiana glauca, Nicotiana noctiflora, Nicotiana cordifolia, Nicotiana knightiana, Nicotiana setchellii and Nicotiana tomentosiformis. For each species, 9.0-22.3Gb high-quality clean data were generated, and 67,073-182,046 transcripts were assembled with lengths greater than 100bp. Over 90% of the ORFs in each species had significant similarity with proteins in the NCBInon-redundantprotein sequence (NR) database. A total of 2491 homologs were identified and used to construct a phylogenetic tree from the respective transcriptomes in Nicotiana. Bioinformatic analysis identified resistance gene analogs, major transcription factor families, and alkaloid transporter genes linked to plant defense.This is the first report on the leaf transcriptomes of six wild Nicotiana species by Illumina paired-end sequencing and de novo assembly without a reference genome. These sequence resources hopefully will provide an opportunity for identifying genes involved in plant defense and several important quality traits in wild Nicotiana and will accelerate functional genomic studies and genetic improvement efforts of Nicotiana or other important Solanaceae crops in the future.


Cai K.,Guizhou Tobacco Research Institute | Xiang Z.,Guizhou Tobacco Research Institute | Zhang J.,Guizhou Tobacco Research Institute | Zhou S.,Guizhou Tobacco Research Institute | And 2 more authors.
Analytical Methods | Year: 2012

A reliable, sensitive and rapid method for determination of eight alkaloids in flue-cured tobacco by dual-column-dual-detector gas chromatography with nitrogen chemiluminescence detection (GC-NCD) has been established. At first, the extraction effects of several factors such as solid to liquid ratio, extraction time, sodium hydroxide solution volume and sodium hydroxide solution mass fraction on the extraction efficiency were simultaneously investigated using an orthogonal array design (OAD). Then, the NCD was compared to other detectors and showed some unique advantages. The analytical method met the strict quantitative requirement by optimizing chromatographic conditions and using dual-column confirmation. Finally, the performance of the proposed method was tested for the extraction and determination of the eight alkaloids in flue-cured tobacco and satisfactory results were achieved. © 2012 The Royal Society of Chemistry.


Chen Y.,Guizhou Tobacco Research Institute | Yin P.-D.,Guizhou Tobacco Research Institute | Chen W.,Guizhou Tobacco Research Institute | Pan W.-J.,Guizhou Tobacco Research Institute
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2013

Plant photosynthate allocation has extremely complex control process, photosynthate allocation mechanism model is one of the main methods to study this complex process. Photosynthate allocation mechanism include: functional equilibrium hypothesis, source-sink relationship hypothesis and allometric relationship hypothesis. This review introduced domestic and international scholar's core thoughts and key formulasabout photosynthate allocation mechanism model based on three hypothesesduring recent years, at the same time, analysed relation of three hypotheses. Three hypotheses describe photosynthate allocation regulation and mechanism from different point of view, so there are not exist in isolation, but complementary, plant through adjust photosynthate allocation to sink to achieve optimal utilization of resources when environmental changes, sinks intensity represents distribution coefficient in the allometric relationship, at the same time sinks contend photosynthate is also different under different environmental conditions and growth stages. This review pointed out the further five research directions.


Cai L.,Guizhou Tobacco Research Institute | Liu Y.,Guizhou Tobacco Research Institute | Meng L.,Guizhou Tobacco Research Institute | Luo Z.,Guizhou Tobacco Research Institute | Shi J.,Guizhou Tobacco Research Institute
Acta Tabacaria Sinica | Year: 2015

Prophages in chromosome of FQY-4 was analyzed on the basis of its complete genomic sequence. Results showed that there was one prophage which contained typical characteristics of phage, and three incomplete prophage elements. Compared with the Ralstonia Solanacearum strain GMI1000, FQY_4 shared similarity in prophage, and homology between the prophage-2 in FQY_4 and prophage φRSA1 was 82%. There were 40 coding sequences with highly significant homology level of more than 99%, which laid solid foundation for diversity analysis of prophage of Ralstonia Solanacearum, and for co-evolution of Ralstonia solanacearum and phages. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


Wenjie P.,Guizhou Tobacco Research Institute | Chaoying J.,Guizhou Tobacco Research Institute | Yuanju T.,Guizhou Tobacco Research Institute | Yang S.X.,University of Guelph
Intelligent Automation and Soft Computing | Year: 2011

Artificial neural network is a powerful tool for modelling and prediction in various fields. Tobacco management specialists need simple and accurate techniques to evaluate tobacco growth and cultivation in the growth period. The objective in this study is to develop a back-propagation neural network (NN) model to accurately predict tobacco growth under various climatic and soil conditions in China. The proposed model used the field test data at 11 locations in main tobacco areas in China. As the output of the NN, the dry weight of tobacco plants was significantly influenced by dry weigh preceding stage, time for sampling, average temperature, relative humidity, soil organic matter and available P value, so these 6 environment factors were taken as the NN input. In the study, an optimal NN model was developed with 6 inputs, 1 output and 19 neurons in the hidden layer. With the same test data, the proposed NN model could perform better than the conventional regression model in terms of the relative error ranges and relative coefficient. © 2011, TSI® Press.


Geng Z.-L.,CAS Kunming Institute of Botany | Geng Z.-L.,Guizhou Tobacco Research Institute | Fang X.,CAS Kunming Institute of Botany | Di Y.-T.,CAS Kunming Institute of Botany | And 3 more authors.
Zeitschrift fur Naturforschung - Section B Journal of Chemical Sciences | Year: 2010

A new limonoid, trichiliton B (1), was isolated from the twigs and leaves of Trichilia connaroides. Its structure was elucidated on the basis of spectroscopic analyses, including application of ESI-MS, HR-ESI-MS, UV, IR, 1D and 2D NMR techniques. © 2010 Verlag der Zeitschrift für Naturforschung, Tübingen.


PubMed | Guizhou Tobacco Research Institute
Type: Journal Article | Journal: Molecular biology reports | Year: 2012

To identify genes that are differentially expressed in tobacco in response to environmental changes and to decipher the mechanisms by which aromatic carotenoids are formed in tobacco, an Agilent Tobacco Gene Expression microarray was adapted for transcriptome comparison of tobacco leaves derived from three cultivated regions of China, Kaiyang (KY), Weining (WN) and Tianzhu (TZ). A total of 1,005 genes were differentially expressed between leaves derived from KY and TZ, 733 between KY and WN, and 517 between TZ and WN. Genes that were upregulated in leaves from WN and TZ tended to be involved in secondary metabolism pathways, and included several carotenoid pathway genes, e.g., NtPYS, NtPDS, and NtLCYE, whereas those that were down-regulated tended to be involved in the response to temperature and light. The expression of 10 differentially expressed genes (DEGs) was evaluated by real-time quantitative polymerase chain reaction (qRT-PCR) and found to be consistent with the microarray data. Gene Ontology and MapMan analyses indicate that the genes that were differentially expressed among the three cultivated regions were associated with the light reaction of photosystem II, response to stimuli, and secondary metabolism. High-performance liquid chromatography (HPLC) analysis showed that leaves derived from KY had the lowest levels of lutein, -carotene, and neoxanthin, whereas the total carotenoid content in leaves from TZ was greatest, a finding that could well be explained by the expression patterns of DEGs in the carotenoid pathway. These results may help elucidate the molecular mechanisms underlying environmental adaptation and accumulation of aroma compounds in tobacco.

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