Guizhou Tea Research Institute

Guiyang, China

Guizhou Tea Research Institute

Guiyang, China
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Yang M.-F.,Guizhou University | Meng Z.-H.,Guizhou University | Meng Z.-H.,Guizhou Tea Research Institute | He Q.,Guizhou University | Dietrich C.H.,University of Illinois at Urbana - Champaign
Zootaxa | Year: 2014

Four new species of Mileewini, Mileewa houhensis Yang, Meng & He, sp. nov., M. xiaofeiae Yang, Meng & He, sp. nov., M. yangi Yang, Meng & He, sp. nov. and Ujna liangae Yang, Meng & He, sp. nov. are described and illustrated. Mileewa nigricauda Yang & Li, 1999 is placed as a junior synonym of M. dorsimaculata (Melichar, 1902); three of the previously described Chinese Mileewa are transferred to the genus Ujna. These are Ujna harpa (Yang & Li, 2004) comb. nov., U. nigrimaculata (Yang & Li, 2004) comb. nov., and U. puerana (Yang & Meng, 2010) comb. nov. M. lynchi (Distant, 1918) is recorded as new for China. A checklist of Mileewini leafhoppers of China along with photograph of their dorsal habitus is provided. Copyright © 2014 Magnolia Press.


Yang M.-F.,Guizhou University | Meng Z.-H.,Guizhou University | Meng Z.-H.,Guizhou Tea Research Institute | Yu X.-F.,Guizhou University
Zootaxa | Year: 2015

Three new species of the genus Anatkina Young, 1986 from China are described and illustrated: A. leishanensis sp. nov., A. alata sp. nov. and A. zhangi sp. nov. The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University, Guiyang, China. In this paper, A. reticulosa Kuoh, 1993 and A. basimacula Kuoh, 1993 are placed as junior synonyms of A. infecta (Distant, 1908) and A. vespertinula (Breddin, 1903), respectively; A. inflammata (Distant, 1908) is recorded as new for China. A key and checklist of all Chinese species of the genus are provided. Copyright © 2015 Magnolia Press.


Yang M.-F.,Guizhou University | Meng Z.-H.,Guizhou University | Meng Z.-H.,Guizhou Tea Research Institute | Li Z.-Z.,Guizhou University
Zootaxa | Year: 2015

A review of the genus Cofana Melichar from China is presented. Ten species are recorded, of which two species are new: C. cheni sp. nov. and C. nii sp. nov. The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (GUGC). In this paper, C. bidentata Krishnankutty & Viraktamath, 2008 is placed as junior synonym of C. yasumatsui Young, 1979. A key of all Chinese species of the genus is provided. Copyright © 2015 Magnolia Press.


Lyu Z.-Y.,Guizhou University | Lyu Z.-Y.,Guizhou Tea Research Institute | Zhi J.-R.,Guizhou University | Zhou Y.-F.,Guizhou Tea Research Institute | And 2 more authors.
Journal of Asia-Pacific Entomology | Year: 2016

In this study, we explored the population genetics of Dendrothrips minowai Priesner, one of the most destructive horticultural pests, in Guizhou Province of China. We have analyzed the data of mitochondrial cytochrome c oxidase subunit I (COI) gene sequences of D. minowai from 27 populations sampled over two years in Guizhou. The results confirmed that the thrips retained moderate levels of genetic diversity (including number of haplotypes, haplotype diversity and nucleotide diversity). Three population groups could be identified according to spatial analysis of molecular variance (SAMOVA). A similar tendency was found according to phylogenetic trees. The AMOVA revealed that > 52% and 38% of variation was attributed to groups and individuals within populations, respectively. Gene flow among populations, estimated using the coalescent method, was relatively high (M = 196.8–825.4). Star-like networks and the demographic analysis (including neutrality testing and mismatch distribution) indicated that thrips underwent a recent population expansion in Guizhou and began to diverge in the Late Pleistocene. The results provided evidence that the eastern region of Guizhou was the source area for the species. © 2016


Meng Z.-H.,Guizhou University | Meng Z.-H.,Guizhou Tea Research Institute | Yang M.-F.,Guizhou University | Webb M.,Natural History Museum in London
ZooKeys | Year: 2014

Newly collected male and female specimens of the leafhopper Kolla albescens Jacobi, 1943 from the type-locality (Northeast China), are identified as Pagaronia albescens (Jacobi), comb. n. (Evacanthinae: Pagaroniini). A redescription of the species is provided together with habitus photographs of the male and female and illustrations of the male and female genitalia. P. continentalis Anufriev, 1970 is placed as a junior synonym of P. albescens syn. n. © Ze-hong Meng et al.


Shen Q.,Guizhou Tea Research Institute | Pan K.,Guizhou Tea Research Institute | Zhang J.,Guizhou Product Quality Supervision and Inspection Institute | Zheng W.-J.,Guizhou Tea Research Institute
Modern Food Science and Technology | Year: 2013

Fresh leaves of bead tea were processed bydifferent spreading method and their leaf appearance, color and main chemical compositions were investigated by sensory evaluation, Hunte-Lab color space method, high performance liquid chromatography and chemical analysis. SPSS (Statistical Product and Service Solutions) statistical software was used for significant difference analysis and correlation analysis. The results showed that during spreading processing of the tea leaf, their moisture content decreased significantly and color was gradually deepened. The leaf became soft and showed delicate fragrance. The most suitable moisture contents and -b/a value for bead tea processing were 74% and 3.66, respectively. Moisture content, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), total amount of catechins and ester type catechins showed a decreasing trend. Tea polyphenols, phenol ammonia ratio, (-)-epicatechin (EC), total amount of catechins presented a downward trend at first then went up with prolonging the processing time. (-)-Epigallocatechin (EGC) decreased first then increased, but decreased again at last period of the spreading processing, while the catechin (C) content increased first, decreased then and increased again lastly. Change of moisture content had a positive correlation with EC, but a negative correlation with soluble sugar content, neither of which was significant. Significant positive correlations of moisture content with phenol ammonia ratio, EGC, EGCG, GCG, ECG, total amount of catechins and ester type catechins, however were found. In particular, the correlation coefficient with EGCG could reach 0.99. In addition, moisture content showed extremely significant negative correlation with contents of amino acids and C.


Shen Q.,Guizhou Tea Research Institute | Pan K.,Guizhou Tea Research Institute | Zheng W.,Guizhou Tea Research Institute | Luo X.,Guizhou Tea Research Institute | Zhang J.,Institute of Products Detection and Determination of Guizhou Province
Journal of Chinese Institute of Food Science and Technology | Year: 2013

Gammaaminobutyric acid and main chemical components of tea samples, which were throughnaerobic and aerobic treatment, were determined by high performance liquid chromatography and physical-chemical compositions detec-tion with SPSS statistical software for analysis of significant difference. The purpose of this article provided a scientific basis for decreasing blood pressure of Gabaron tea. The results showed that GABA had low levels of tea leaf in between 0.041 mg/g and 0.061 mg/g, while GABA had significantly increased after anaerobic and aerobic treatment in between 1.37 mg/g and 1.81 mg/g. There were microwave fixation, roller fixation, water fixation, pan fixation and steam fixation to compare different fixation effects for tea samples. GABA and main chemical compositions of tea samples were different through five kinds of fixation methods. GABA of the microwave fixation tea samples, which increased by 28.7 times on control tea samples, retained the maximum and amounted for 1.81 mg/g. Followed by froller fixation and steam fixation; water fixation and pan fixation were the worst. Amino acid and tea polyphenols retention through microwave fixation and steam fixationwere than higher the other three fixation methods. The amount of poor retention of amino acid and tea polyphenols was water fixation. Microwave fixation was conducive to the retention tea quality, but various combinations of fixation methods should be further studied.


Liu S.-C.,Chinese Academy of Agricultural Sciences | Liu S.-C.,Guizhou Tea Research Institute | Jin J.-Q.,Chinese Academy of Agricultural Sciences | Ma J.-Q.,Chinese Academy of Agricultural Sciences | And 5 more authors.
PLoS ONE | Year: 2016

Tea plant (Camellia sinensis) is an economically important beverage crop. Drought stress (DS) seriously limits the growth and development of tea plant, thus affecting crop yield and quality. To elucidate the molecular mechanisms of tea plant responding to DS, we performed transcriptomic analysis of tea plant during the three stages [control (CK) and during DS, and recovery (RC) after DS] using RNA sequencing (RNA-Seq). Totally 378.08 million high-quality trimmed reads were obtained and assembled into 59,674 unigenes, which were extensively annotated. There were 5,955 differentially expressed genes (DEGs) among the three stages. Among them, 3,948 and 1,673 DEGs were up-regulated under DS and RC, respectively. RNA-Seq data were further confirmed by qRT-PCR analysis. Genes involved in abscisic acid (ABA), ethylene, and jasmonic acid biosynthesis and signaling were generally up-regulated under DS and down-regulated during RC. Tea plant potentially used an exchange pathway for biosynthesis of indole-3-acetic acid (IAA) and salicylic acid under DS. IAA signaling was possibly decreased under DS but increased after RC. Genes encoding enzymes involved in cytokinin synthesis were up-regulated under DS, but downregulated during RC. It seemed probable that cytokinin signaling was slightly enhanced under DS. In total, 762 and 950 protein kinases belonging to 26 families were differentially expressed during DS and RC, respectively. Overall, 547 and 604 transcription factor (TF) genes belonging to 58 families were induced in the DS vs. CK and RC vs. DS libraries, respectively. Most members of the 12 TF families were up-regulated under DS. Under DS, genes related to starch synthesis were down-regulated, while those related to starch decomposition were up-regulated. Mannitol, trehalose and sucrose synthesis-related genes were up-regulated under DS. Proline was probably mainly biosynthesized from glutamate under DS and RC. The mechanism by which ABA regulated stomatal movement under DS and RC was partly clarified. These results document the global and novel responses of tea plant during DS and RC. These data will serve as a valuable resource for drought-tolerance research and will be useful for breeding drought-resistant tea cultivars. © 2016 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Liu S.-C.,Chinese Academy of Agricultural Sciences | Liu S.-C.,Guizhou Tea Research Institute | Yao M.-Z.,Chinese Academy of Agricultural Sciences | Ma C.-L.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Scientia Horticulturae | Year: 2015

Drought is one of the major constraints for crop growth and productivity worldwide. Here, responses to soil drying and rewatering were measured at morphological, physiological and molecular levels in two adult field-grown tea plant [Camellia sinensis (L.) O. Kuntze] cultivars including drought-susceptible 'Zhuyeqi' (T1) and drought-tolerant 'Ningzhou 2' (T2). After eight days drought stress (DS), most of leaves in T1 were reddish brown, curled and withered, whereas only a few needle spots and yellow patches were observed in T2. Based on the morphological symptoms, T2 recovered more quickly than T1. The malondialdehyde (MDA), soluble sugars (SS) and proline (Pro) contents as well as superoxide dismutase (SOD) and catalase (CAT) activities in two cultivars increased significantly as DS progressed and then rapidly decreased following rehydration. In contrast, the abscisic acid (ABA) and salicylic acid (SA) content peaked in the early stage of DS and then decreased rapidly, while these changes were more apparent in T2 than in T1. T1 had a higher concentration of MDA, SS and Pro than T2 throughout dehydration and rehydration. T2 was characterized by lower ABA and higher SA accumulation, but the opposite results were observed in T1. Furthermore, T2 had stable SOD and higher CAT activities during the stress and recovery. Under recovery rewatering, two cultivars still maintained high CAT activities. SS level in T1 was 1.2 times higher than control value on the fourth day after rehydration, while in T2 it was nearly equal to control level. In general, T2 showed more drastic changes in the expression of five selected genes during and after DS, these changes were positively correlated with corresponding physiological indicators. Nevertheless, expression levels of δ1-pyrroline-5-carboxylate synthetase (P5CS) gene and phenylalanine ammonia lyase (PAL) gene in T1 were subjected to feedback inhibition. Overall, these findings were consistent with the results from the controlled indoor test and duplicate field test, providing new insights into the drought-tolerant mechanisms in tea plants. © 2015 Elsevier B.V.

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