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Lv Y.-S.,Fujian Medical University | Zhou H.-L.,Fujian Medical University | Mao H.-P.,Fujian Medical University | Gao R.,Fujian Medical University | And 2 more authors.
International Urogynecology Journal and Pelvic Floor Dysfunction | Year: 2012

Introduction and hypothesis: Intravesical instillation of hyaluronic acid (HA) may restore the integrity of glycosaminoglycan layer in patients with painful bladder syndrome/interstitial cystitis (PBS/IC), and the benefit may be improved with addition of alkalinized lidocaine (AL). Methods: 48 women with severe PBS/IC who failed oral medications were enrolled and divided into one trial and two control groups. The trial group received intravesical 40 mg HA, 10 ml of 2 % lidocaine and 5 ml of 8.4 % sodium bicarbonate on a weekly basis for 8 weeks and then monthly for 4 months with a subsequent follow-up of 24 weeks, while the two control groups received 40 mg HA and mixture of 10 ml of 2 % lidocaine and 5 ml of 8.4%sodium bicarbonate respectively following the same procedure. Response to therapy was evaluated by Global Response Assessment, voids per day, Visual Analogue Scale for pain, frequency and urgency, O'leary-Sant Interstitial Cystitis Symptom Index and Problem Index, cystoscopy and bladder capacity. Results: Overall 45 patients finished this study protocol. The HA + AL group and the AL group showed significant improvement at week 2 (P < 0.01), while the HA group began to show effect at week 4 (P < 0.01). There was no improvement in the AL group at week 24 and these patients quitted the study without follow up. Contrarily, the HA + AL and HA group kept on improving till the end of the study without significant difference between the two groups. Conclusions: Intravesical instillation of HA and AL may provide both immediate and sustained relief of symptoms in severe PBS/IC in this preliminary study. © 2012 The International Urogynecological Association.

Jie D.,Guizhou Provincial Peoples Hospital | Zhongmin Z.,Guizhou Provincial Peoples Hospital | Guoqing L.,Central South University | Sheng L.,Central South University | And 3 more authors.
Digestive Diseases and Sciences | Year: 2013

Background: The first identified lysine-specific demethylase, LSD1, plays an important role in the metastatic progression of several types of cancer. Aims: The aim of this study was to investigate LSD1, E-cadherin, and N-cadherin expression in colon cancer specimens and their clinical significance. Methods: The expression of LSD1, E-cadherin, and N-cadherin in colon cancer specimens was determined by immunohistochemistry, and the relationship between the expression of the respective molecules and clinicopathological characteristics was analyzed. Results: The positive expression rates of LSD1, E-cadherin, and N-cadherin in colon cancer specimens were 66.7 % (72/108), 85.2 % (92/108), and 41.7 % (45/108), respectively. LSD1 was significantly more highly expressed in colon cancer specimens classified as high TNM stage lesions and with distant metastasis (P < 0.05). Further analysis demonstrated that LSD1 expression was positively correlated with lymph node and distant metastases (P < 0.05). However, E-cadherin expression was significantly downregulated in colon cancer specimens classified as high TNM stage lesions and with distant metastasis (P < 0.05), whereas the expression of N-cadherin did not differ significantly according to clinical and pathological characteristics (P > 0.05). Correlation analysis revealed that LSD1 expression was negatively correlated with E-cadherin expression (r s = -0.318, P = 0.001), but not evidently correlated with N-cadherin expression (r s = 0.182, P = 0.06). Colon cancer specimens with positive LSD1 expression and negative E-cadherin expression were correlated with significantly lower overall survival. Conclusions: LSD1 showed a significantly higher expression, in contrast to the significantly lower expression of E-cadherin, in colon cancer specimens classified as high TNM stage lesions and with distant metastasis. Positive expression of LSD1 and negative expression of E-cadherin may be predictors of a worse colon cancer prognosis. © 2013 Springer Science+Business Media New York.

Du F.-W.,Guizhou Provincial Peoples Hospital
Journal of Interventional Radiology (China) | Year: 2016

Objective: To analyze the risk factors and prognosis of patients with coronary stent thrombosis (ST) after receiving percutaneous coronary drug - eluting stent implantation. Methods: Clinical data of 39 patients with ST, who were encountered at author's hospital during the period from July 2006 to March 2012, were retrospectively analyzed. With 1:2 pairing selection, 78 patients with no stent restenosis on coronary angiography encountered during the same period were collected and used as the control group. The risk factors of postoperative stent thrombosis were analyzed. Results: Acute myocardial infarction (AMI) as the initial onset, the smaller stent diameter (≤2.94 mm), the longer length (>29.5 mm) and the lower (>50%) preoperative left ventricular ejection fraction (EF) were related to the occurrence of stent thrombosis. Emergency coronary intervention was carried out once more in 33 patients, of whom one patient died during hospitalization period and the remaining 32 patients recovered at the time of discharge. Conclusion AMI as initial onset, smaller stent diameter, longer stent length and lower preoperative EF (>50%) are independent predictors of coronary stent thrombosis. Stent length ≥29.5 mm and stent diameter ≤2.94 mm are associated with higher occurrence of coronary stent thrombosis. Clinically, most patients with coronary stent thrombosis after receiving percutaneous coronary drug-eluting stent implantation are manifested as AMI. The mortality is higher when stent thrombosis occurs. For such patients emergency coronary intervention is safe and effective.

Tian M.-L.,Guizhou Provincial Peoples Hospital | Shen Y.,Guizhou Provincial Peoples Hospital | Sun Z.-L.,Guizhou Provincial Peoples Hospital | Zha Y.,Guizhou Provincial Peoples Hospital
International Urology and Nephrology | Year: 2015

Objective: Pentoxifylline (PTF) has anti-inflammatory properties, which may be beneficial for diabetic nephropathy (DN). A meta-analysis was conducted to assess the additive effect of pentoxifylline and its safety among patients with type 2 DN under blockade of angiotensin system. Data sources: Relevant studies were searched from PubMed, CBM, EMBASE, CENTRAL and Cochrane renal group specialized register. Selection criteria: All RCTs that compared the benefits and harms of pentoxifylline and ACEI/ARB with ACEI/ARB alone for DN were included. Data extraction and analysis: Pertinent data were extracted independently by two authors. Meta-analyses were performed when more than one study provided data on a comparable outcome. Standard mean differences (SMDs) for proteinuria and albuminuria, mean differences (MDs) for systolic blood pressure (SBP), diastolic blood pressure (DBP), HbA1c, serum creatinine (Scr), creatinine clearance (CrCl) and urine tumor necrosis factor-alpha (UTNF-α), 95 % confidence intervals (CIs) were calculated, and heterogeneity was assessed with the I2 test. Adverse effects were assessed using descriptive techniques. Results: Eight studies including 587 patients with a median duration of 5 months were identified. Compared with ACEI/ARB alone, the combination of PTF and ACEI/ARB significantly reduced proteinuria (SMD 0.76, 95 % CI 0.52–0.99), albuminuria (SMD 0.36, 95 % CI 0.12–0.59) and UTNF-α (MD 1.56 ng/g, 95 % CI 0.09–3.03). However, no statistically significant changes were observed for SBP, DBP, HbA1c, Scr and CrCl. The most frequent adverse effects in patients treated with PTF were gastrointestinal symptoms (28/298) and dizziness (7/298), but in most cases, these symptoms were mild, only six participants withdrew due to intractable nausea and vomiting. Conclusions: Pentoxifylline can significantly provide additive antiproteinuric effect independent from the decrease in BP or improvement in glycemic control in DN patients under blockade of angiotensin system. Further large, multicenter, high-quality studies with long duration are necessary to prove whether it really has renoprotective effects in this patient population. © 2015, Springer Science+Business Media Dordrecht.

Xiong Y.,Guizhou Provincial Peoples Hospital
Zhonghua yi xue za zhi | Year: 2014

OBJECTIVE: To explore the surgical efficacy of lesions in mesencephalic aqueduct and around it with neuroendoscope.METHODS: A total of 14 cases with lesion underwent neuroendoscope. Endoscopic third ventriculostomy (ETV), excision or biopsy and mesencephalic aqueduct plasty were performed.RESULTS: There were primary obstruction (n = 4), glioma (n = 4), intraventricular cysticercosis (n = 2), pineal cell tumor (n = 1), lymphoma (n = 1), brain abscess (n = 1) and germinoma (n = 1). Postoperative cranial computed tomography (CT) and/or magnetic resonance imaging (MRI) showed narrowed ventricle. The outcomes were symptomatic improvement (n = 5), no change (n = 5), worsening (n = 4) and mortality (n = 0).CONCLUSION: ETV, lesion biopsy or partial lesion resection and aqueductal plasty are efficacious for the patients with lesions in mesencephalic aqueduct and around it.

Ding J.,Guizhou Provincial Peoples Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2011

To evaluate the necessity of splenectomy in radical resection of gastric cancer. Twelve studies comparing outcomes after radical resection of gastric cancer with or without splenectomy were identified. Both fixed effect model and random effect model were used. There were 2628 patients in total. There were significant differences in complications between splenectomy group and spleen-preserving group(OR=1.91, 95% CI:1.28-2.87, P<0.05), while no significant difference in 5-year survival rate was noticed(HR=0.90, 95% CI:0.73-1.11, P>0.05). Radical resection of gastric cancer combined with splenectomy is not associated with improved survival but increased postoperative complications.

Min L.,Guizhou Provincial Peoples Hospital | Ying C.,Guizhou Provincial Peoples Hospital | Anzhi W.,Guizhou Provincial Peoples Hospital
Cancer Research and Clinic | Year: 2014

Objective: To investigate the correlation between patient's age and expression of Ki-67 or microvessel density in primary hepatocellular carcinoma. Methods: The expression level of Ki-67 and the microvessel density in the hepatocellular carcinoma tissues were detected by immunohistochemical method, and the correlation between age and expression of Ki-67 or microvessel density were analyzed. Results: In 48 cases of hepatocellular carcinoma tissues, Ki-67 expressed to varying degrees, but the positive rate was 83.3% (40/45), and there was no expression in corresponding noncancerous tissues (P < 0.01). Microvessel density marked by CD34 in cancer tissues and noncancerous tissues were 32.17±8.06 and 10.83±2.85 respectively. Microvessel density of cancer tissues was higher than noncancerous tissues (P < 0.01). Both levels of Ki-67 expression and microvessel density in hepatocellular carcinoma were negatively correlated with the age (r = -0.418, P = 0.003 and r = -0.443, P = 0.002 respectively). Conclusion: Both levels of Ki-67 expression and microvessel density in hepatocellular carcinoma are negatively correlated with patient's age, which may suggest worse prognosis for the younger patients.

Ding J.,Guizhou Provincial Peoples Hospital | Ding J.,Central South University | Zhang Z.-M.,Guizhou Provincial Peoples Hospital | Xia Y.,Guizhou Provincial Peoples Hospital | And 5 more authors.
British Journal of Cancer | Year: 2013

Background: Emerging evidence has demonstrated that lysine-specific demethylase 1 (LSD1) has an important role in many pathological processes of cancer cells, such as carcinogenesis, proliferation and metastasis. In this study, we characterised the role and molecular mechanisms of LSD1 in proliferation and metastasis of colon cancer. Methods: We evaluated the correlation of LSD1, CDH-1 and CDH-2 with invasiveness of colon cancer cells, and investigated the roles of LSD1 in proliferation, invasion and apoptosis of colon cancer cells. We further investigated the mechanisms of LSD1-mediated metastasis of colon cancer. Results: Lysine-specific demethylase 1 was upregulated in colon cancer tissues, and the high LSD1 expression was significantly associated with tumour-node-metastasis (TNM) stages and distant metastasis. Functionally, inhibition of LSD1 impaired proliferation and invasiveness, and induced apoptosis of colon cancer cells in vitro. The LSD1 physically interacted with the promoter of CDH-1 and decreased dimethyl histone H3 lysine 4 (H3K4) at this region, downregulated CDH-1 expression, and consequently contributed to colon cancer metastasis. Conclusion: Lysine-specific demethylase 1 downregulates the expression of CDH-1 by epigenetic modification, and consequently promotes metastasis of colon cancer cells. The LSD1 antagonists might be a useful strategy to suppress metastasis of colon cancer. © 2013 Cancer Research UK. All rights reserved.

Zha Y.,Guizhou Provincial Peoples Hospital
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2013

To investigate the effect of continuous renal replacement therapy (CRRT) on the outcome of severe pneumonia in patients receiving long-term immunosuppressants. Thirty-four patients, who had been treated with long-term immunosuppressants, were admitted for severe pneumonia. After admission, the dose of immunosuppressants including glucocorticoids was decreased, and the patients were divided into 2 groups: antibiotic treatment group (n = 16) and antibiotic + CRRT treatment group (n = 18). Before and after treatment, the changes of the patients' condition, chest CT and blood gas analysis were monitored. Biomarkers including C-reactive protein (CRP), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8(IL-8), white blood cell and neutrophil counts were determined. After treatment, the levels of CRP[(109 ± 24) vs (40 ± 13) mg/L], IFN-γ [(151 ± 28) vs (42 ± 12) ng/L], TNF-α [(301 ± 45) vs (118 ± 28) pg/L], IL-6 [(214 ± 45) vs (76 ± 23) pg/L], IL-8[(590 ± 121) vs (159 ± 60) pg/L], white blood cell count [(14.3 ± 5.7)×109/L vs (8.5 ± 2.7)×109/L], and neutrophil percentage [(91.3 ± 3.1)% vs (75.3 ± 2.6)%] decreased significantly in the antibiotic + CRRT group (P < 0.05) as compared to the antibiotic treatment group. In the antibiotic + CRRT group, blood gas showed significant improvement in pH [(7.30 ± 0.12) to (7.37 ± 0.18)], SaO2 [(80.6 ± 7.6)% to (91.9 ± 7.3)%] and PaO2 (41 ± 6) mm Hg (1 mm Hg = 0.133 kPa) to (71 ± 9) mm Hg. The patients' condition and chest CT abnormalities also improved more rapidly in the antibiotic + CRRT group. The 6-month survival was increased by 12.9% in the antibiotic + CRRT group as compared to the antibiotic group (P < 0.05). CRRT is effective in clearance of inflammatory mediators and may increase survival of severe pneumonia patients receiving long-term treatment of immunosuppressants.

Deng Y.K.,Guizhou Provincial Peoples Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To investigate the erythrocyte protective effects of Ginaton, a ginkgo biloba extract, in patients undergoing hypothermic cardiopulmonary bypass (CPB). Sixty patients, who suffered from rheumatic heart disease of ASA grade II-III and scheduled for mitral valve replacement with intravenous anesthesia, were randomly assigned to two groups equally, the Ginaton group and the control group. They were administered with Ginaton 1 mg/kg and saline respectively via intravenous dripping before open heart surgery before beginning CPB. Blood samples were taken from radial artery at different time points, i.e., before CPB (T1), nasopharyngeal temperature (30-31 degrees C) stabilized stage (T2), nasopharyngeal temperature restoration (36 degrees C) stage (T3), 30 min after CPB (T4) and 3 h after CPB (T5), for determination of malondialdehyde (MDA) and superoxide dismutase (SOD) levels in plasma and erythrocyte (P-MDA, E-MDA, P-SOD and E-SOD), as well as the Na+ -K+ -ATPase and Ca+ -Mg2+ -ATPase activities in erythrocytes. As compared with those at T1, in the control group, P-MDA, E-MDA, and E-SOD at T2-T5 and E-SOD at T2 were higher, but E-SOD at T3-T5 were lower (P < 0.01); while in the Ginaton group P-MDA, E-MDA, and E-SOD at T3-T4 were higher (P < 0.05 or P < 0.01). As compared with those in the control group, the levels of P-MDA and E-MDA at T2-T5 were significantly lower, and E-SOD at T3-T5 were higher (P < 0.05 or P < 0.01). Activities of Na+ -K+ -ATPase and Ca+ -Mg2+ -ATPase significantly increased at T2 and gradually decreased after then in both groups (P < 0.05 or P < 0.01), but those at T2-T5 were significantly higher in Ginaton group than in control group (P < 0.05 or P < 0.01). Ginaton displays an erythrocyte protecting effect by way of alleviating the lipid peroxidation in erythrocytes' membrane.

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