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Zhang R.,Guizhou University | Zhang R.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | He L.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | He L.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | And 5 more authors.
Korean Journal for Food Science of Animal Resources | Year: 2016

Cardiovascular and cerebrovascular diseases seriously harm human health, and Bifidobacterium is the most beneficial probiotic in the gastrointestinal tract of humans. This work aimed to screen cholesterol-lowering Bifidobacterium from Guizhou Xiang Pig and evaluate its tolerance to oxygen, acid, and bile. Twenty-seven aerotolerant strains with similar colony to Bifidobacterium were isolated through incubation at 37°C in 20% (v/v) CO2-80% (v/v) atmospheric air by using Mupirocin lithium modified MRS agar medium, modified PTYG with added CaCO3, and modified PTYG supplemented with X-gal. Ten strains with cholesterol-lowering rates above 20% (w/w) were used for further screening. The selected strains' tolerance to acid and bile was then determined. A combination of colony and cell morphology, physiological, and biochemical experiments, as well as 16S rRNA gene-sequence analysis, was performed. Results suggested that BZ25 with excellent characteristics of high cholesterol-removal rate of 36.32% (w/w), as well as tolerance to acid and bile, was identified as Bifidobacterium animalis subsp. lactis. To further evaluate Bifidobacterium BZ25's growth characteristic and tolerance to oxygen, culture experiments were performed in liquid medium and an agar plate. Findings suggested that BZ25 grew well both in environmental 20% (v/v) CO2-80% (v/v) atmospheric air and in 100% atmospheric air because BZ25 reached an absorbance of 1.185 at 600 nm in 100% atmospheric air. Moreover, BZ25 was aerotolerant and can grow in an agar medium under the environmental condition of 100% atmospheric air. This study can lay a preliminary foundation for the potential industrial applications of BZ25. © 2016 Korean Society for Food Science of Animal Recources. Source

Wu Y.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy
Microchimica Acta | Year: 2015

We report on an “off-on” type of fluorescent assay for silver(I) ion determination that is based on the use of silver-specific DNA, gold nanoparticles, and Rhodamine B (RhoB). The random coil cytosine-rich silver-specific DNA undergoes structural transformation on capturing Ag(I), and this results in a change in the aggregation status of the gold nanoparticles and simultaneously affects the fluorescence intensity of RhoB that was added to the solution. The fluorescence intensity of RhoB is related to the concentration of Ag(I) in the 2.73 to 1500 ppb range, and the detection limit is 2.73 ppb. The method was applied to the determination of Ag(I) in (spiked) water samples. Conceivably, it also may be applied to fluorescence imaging silver ions and, by using other ion-specific DNAs, to detect other metal ions. [Figure not available: see fulltext.] © 2015, Springer-Verlag Wien. Source

Zhou H.-J.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | Zhou H.-J.,Guizhou University | He L.-P.,Guizhou University | He L.-P.,Key Laboratory of Agricultural and Animal Products | And 6 more authors.
Journal of Pure and Applied Microbiology | Year: 2014

The main purpose of the work was to screen intracellular lipase-producing microbial with high selective synthesis of 1,3-diolein, a healthy lipid. The screening model is a combination of Rhodamine B-olive oil agar plat, para-nitrophenyl palmitate (ρ-NPP) for ester synthetase activity and HPLC analysis of products of glycerolysis of triolein. The results show that the strain GZUF36 (CCTCC No. M2012538) can be used to catalyze the selective synthesis of 1,3-diolein. 1,3-Diolein yield is 11.12% (w/w) by whole-cell of GZUF36 catalyzed glycerolysis and it is accounted for 72% of total diolein (including 1,3- and 1,2-isomer). Then, GZUF36 was identified as Aspergillus niger by morphology and 18S rDNA sequence analysis. It is also suggested that the model can be applied to screen other lipases with high ester synthetase activities. Source

Li C.-Q.,Guizhou University | Xia C.-S.,Guizhou University | Xia C.-S.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | He L.-P.,Guizhou University | And 6 more authors.
Journal of Pure and Applied Microbiology | Year: 2014

Main medium components that influence whole-cell lipase from A. Niger GZUF36 catalyzing synthesis of 1,3-diglyceride (1,3-DG) by glycerolysis of triglyceride (TG) are identified by Plackett-Burman (PB) design. The response index was yield of DG synthesized by the lipase-catalyzed glycerolysis. The selected factors for PB design included glucose, soybean meal, zinc sulfate, disodium hydrogen phosphate, calcium chloride, potassium chloride and potassium hydrogen phosphate. The results showed that the main medium constituents were glucose, soybean meal and potassium chloride. Other factors were not significant. On the base of PB design, the optimized medium was obtained, with which the yield of diglyceride (DG) was increased 0.78-fold compared with basal medium. The synthesized OG can be used as healthy lipid. This study would improve the further optimization steps on the bioprocess development tracks. Source

Niu X.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | Niu X.,Guizhou University | Qiu S.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | Qiu S.,Guizhou University | And 4 more authors.
Frontiers of Chemical Engineering in China | Year: 2011

The solid-state prefermentation by Aspergillus niger (CICC 2377) and Aspergillus flavus (CICC 40536) was employed to increase the oil extraction yield from soybean. The influence of incubation time on oil yield was investigated. The maximum oil yield extracted from the substrate prefermented by Aspergillus niger for 96 h was 23%, which increased by 47.4% compared with control (15.6%). In the same fermentation conditions, the maximum oil yield extracted from substrate prefermented by Aspergillus flavus was 21.6%, which increased by 38.5% compared with control (15.6%). The quality of soybean oil was not changed obviously by the pretreatment of fermentation with fungi. © Higher Education Press and Springer-Verlag Berlin Heidelberg 2010. Source

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