Guizhou Key Laboratory of Agricultural Biotechnology

Guiyang, China

Guizhou Key Laboratory of Agricultural Biotechnology

Guiyang, China
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Gui Y.,Huazhong Agricultural University | Gui Y.,Guizhou Provincial Institute of Crop Germplasm Resources | Gui Y.,Guizhou Key Laboratory of Agricultural Biotechnology | Zhu G.-S.,Guizhou Provincial Institute of Crop Germplasm Resources | And 10 more authors.
Fungal Biology | Year: 2015

Agaricus is a genus of saprobic basidiomycetes with more than 400 species recognized worldwide, with about 50 species known in China. Our objective was to investigate three new species of section Arvenses in highland subtropical Southwest China. Agaricus guizhouensis is a new species characterized by a white pileus with yellowish squamules, small ellipsoid spores and cheilocystidia with yellowish-brown pigments; another new species, Agaricus longistipes is recognized by its slender stipe, and its elongate-ellipsoid basidiospores; the third new one, Agaricus megalocarpus is remarkable by its large size and its pileus surface covered with fine brown squamules. It is firstly reported for Guizhou Province that Agaricus abruptibulbus, Agaricus flocculosipes, and Agaricus subrufescens are illustrated. Two probable new species require further studying. A phylogenetic analyses of rDNA-ITS sequence data belonging to section Arvenses showed that the section Arvenses is monophyletic and can be subdivided in five branches, the branch of A. subrufescens and four clades (A-D). The eight species from highland subtropical Southwest China were distributed in all five branches, indicating that this highland is at the climatic crossroads. The white pileus trait and the potential interest are discussed. These data suggest a potential species richness that remains to be discovered. © 2014 The British Mycological Society.

Chen Y.M.,Kunming University of Science and Technology | Liu Y.X.,Guizhou Key Laboratory of Agricultural Biotechnology | Liu Y.X.,Guizhou Academy of Agricultural Science | Liu Z.Y.,Guizhou Key Laboratory of Agricultural Biotechnology | And 2 more authors.
Applied Mechanics and Materials | Year: 2014

Shiraia bambusicola is a medicinal fungus growing on the twigs of bamboo plants, with the higher value in medicinal use. The natural resource of S. bambusicola has rapidly reduced recently because of the large-scale over-collection and the habitat destruction. The resource would be in extinction if there are no any protective measures and availability of sustainable use strategies. In this paper it is summarized that the application and development of S. bambusicola. A comprehensive analysis on the progress of research as well as the current problems in application and the future outlook are also proposed in the paper. © (2014) Trans Tech Publications, Switzerland.

Peng L.,Guizhou University | Yang Y.,Liupanshui Teachers College | Hyde K.D.,Mae Fah Luang University | Bahkali A.H.,King Saud University | And 2 more authors.
Cryptogamie, Mycologie | Year: 2012

Thirty-eight strains of Colletotrichum were isolated from diseased Citrus leaves in Guizhou and Yunnan provinces in China. Based on morphological characters and multilocus sequence data, the strains were identified as C. boninense (1 strain), C. brevispora (1), C. fructicola (2), C. gloeosporioides (29), C. karstii (2), C. simmondsii (1) and Colletotrichum murrayae; the latter represents a new species which is described in this paper. Colletotrichum gloeosporioides was originally isolated from Citrus sinensis in southern Italy and was the most common species associated with diseased leaves of Citrus in China. © 2012 Adac. Tous droits réservés.

Tao G.,CAS Institute of Microbiology | Tao G.,Guizhou Institute of Plant Protection | Tao G.,Guizhou Key Laboratory of Agricultural Biotechnology | Hyde K.D.,Mae Fah Luang University | Cai L.,CAS Institute of Microbiology
Journal of Applied Microbiology | Year: 2013

Colletotrichum kahawae is a strongly aggressive pathogen causing coffee berry disease and is specific to Arabica coffee (Coffea arabica) in Africa. In this article, we developed a real-time PCR assay for the species-specific diagnosis of C. kahawae by designing the primers and a TaqMan probe derived from the single nucleotide polymorphism-rich region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Methods and Results: DNA markers from rDNA internal transcribed spacer, actin, β-tubulin and GAPDH genes of the ex-type culture of C. kahawae and 10 reference strains of Colletotrichum species were analysed for intra- and interspecific variations. The GAPDH gene was selected to develop a species-specific DNA marker. A TaqMan real-time PCR assay for species-specific detection of C. kahawae was developed, and its accuracy was tested against type strains of other phylogenetically closely related species in the C. gloeosporioides species complex, with the detection sensitivity of 80fgμl-1 of genomic DNA. Conclusions: This real-time PCR assay is highly specific and sensitive for the diagnosis of C. kahawae and can be applied in qualitative and quantitative tests. Significance and Impact of the Study: This protocol allows for a rapid and sensitive detection of C. kahawae and will be useful in disease management and pest detection to prevent further spread of this pathogen. © 2012 The Society for Applied Microbiology.

Peng L.-J.,Guizhou University | Sun T.,Chongqing Entry Exit Inspection and Quarantine Bureau | Yang Y.-L.,Liupanshui Normal University | Cai L.,CAS Institute of Microbiology | And 4 more authors.
Mycoscience | Year: 2013

Twenty-six strains representing three species of Colletotrichum were isolated from leaf and fruit lesions of vitaceous plants in Guizhou and Yunnan provinces, China. The strains were characterized by morphology and phylogenetic analyses of actin, β-tubulin, calmodulin, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase and rDNA internal transcribed spacer gene sequences. The combined dataset showed that 20 of 26 strains represented a novel species, the rest being Colletotrichum fructicola (four strains) and Colletotrichum gloeosporioides (two strains). The new species is described herein as Colletotrichum viniferum. Its conidia, compared with similar Colletotrichum species are cylindrical and 12e 16 mm long. Based on pathogenicity tests, C. viniferum caused leaf spots and anthracnose of table grape but was not host-specific. © 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.

PubMed | Big Genome Institute, Guizhou University, Mae Fah Luang University, Guizhou Key Laboratory of Agricultural Biotechnology and CAS Institute of Microbiology
Type: | Journal: BMC genomics | Year: 2016

Aspergillus cristatus is the dominant fungus involved in the fermentation of Chinese Fuzhuan brick tea. Aspergillus cristatus is a homothallic fungus that undergoes a sexual stage without asexual conidiation when cultured in hypotonic medium. The asexual stage is induced by a high salt concentration, which completely inhibits sexual development. The taxon is therefore appropriate for investigating the mechanisms of asexual and sexual reproduction in fungi. In this study, de novo genome sequencing and analysis of transcriptomes during culture under high- and low-osmolarity conditions were performed. These analyses facilitated investigation of the evolution of mating-type genes, which determine the mode of sexual reproduction, in A. cristatus, the response of the high-osmolarity glycerol (HOG) pathway to osmotic stimulation, and the detection of mycotoxins and evaluation of the relationship with the location of the encoding genes.The A. cristatus genome comprised 27.9 Mb and included 68 scaffolds, from which 10,136 protein-coding gene models were predicted. A phylogenetic analysis suggested a considerable phylogenetic distance between A. cristatus and A. nidulans. Comparison of the mating-type gene loci among Aspergillus species indicated that the mode in A. cristatus differs from those in other Aspergillus species. The components of the HOG pathway were conserved in the genome of A. cristatus. Differential gene expression analysis in A. cristatus using RNA-Seq demonstrated that the expression of most genes in the HOG pathway was unaffected by osmotic pressure. No gene clusters associated with the production of carcinogens were detected.A model of the mating-type locus in A. cristatus is reported for the first time. Aspergillus cristatus has evolved various mechanisms to cope with high osmotic stress. As a fungus associated with Fuzhuan tea, it is considered to be safe under low- and high-osmolarity conditions.

Tao G.,CAS Institute of Microbiology | Tao G.,Guizhou Institute of Plant Protection | Tao G.,Guizhou Key Laboratory of Agricultural Biotechnology | Cai L.,CAS Institute of Microbiology
Journal of Plant Pathology | Year: 2013

Colletotrichum kahawae, a very aggressive pathogen causing coffee berry disease (CBD), is specific to Arabica coffee (Coffea arabica) in Africa. This species is a significant quarantine pathogen in many countries which are still free from CBD. C. kahawae is morphologically similar to C. gloeosporioides but is phylogenetically and phenotypically distinct. Loop-mediated isothermal amplification (LAMP) is a simple, cost-effective, and rapid method for specific DNA-based detection. In this paper, the Apn2/ MAT locus was selected as candidate marker, on whose basis species-specific primers were designed for loopmediated isothermal amplification (LAMP) diagnosis of C. kahawae. The accuracy of this assay was tested using the type strains of other closely related species in the C. gloeosporioides species complex. The sensitivity of LAMP is high, with 8×10-2 pg μl-1 genomic DNA as the lowest detectable concentration. It offers a new and effective way for the rapid, specific and cost-effective diagnosis of severe fungal pathogens, thus representing a favourable option to conventional PCR and real-time PCR assays. LAMP diagnosis of C. kahawae is likely to provide a useful technological option for quarantine and disease management and control to prevent further spreading of this pathogen.

Tao G.,CAS Institute of Microbiology | Tao G.,Guizhou Institute of Plant Protection | Tao G.,Guizhou Key Laboratory of Agricultural Biotechnology | Liu Z.-Y.,Guizhou Key Laboratory of Agricultural Biotechnology | And 3 more authors.
Fungal Diversity | Year: 2013

Thirty-six strains of endophytic Colletotrichum species were isolated from leaves of Bletilla ochracea Schltr. (Orchidaceae) collected from 5 sites in Guizhou, China. Seventeen different species, including 7 new species (namely C. bletillum, C. caudasporum, C. duyunensis, C. endophytum, C. excelsum-altitudum and C. guizhouensis and C. ochracea), 8 previously described species (C. boninense, C. cereale, C. destructivum, C. karstii, C. liriopes, C. miscanthi, C. parsonsiae and C. tofieldiae) and 2 sterile mycelia were identified. All of the taxa were identified based on morphology and phylogeny inferred from multi-locus sequences, including the nuclear ribosomal internal transcribed spacer (ITS) region, partial genes of β-tubulin (TUB2), actin (ACT) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Comprehensive morphological descriptions and illustrations are provided for new species. Our investigation indicates a high diversity of Colletotrichum species in B. ochracea. © 2013 Mushroom Research Foundation.

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