Time filter

Source Type

Zhou X.,Guizhou Normal University | Zhou X.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | Zhao Y.,Guizhou Normal University | Zhao Y.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | And 5 more authors.
Journal of Separation Science | Year: 2010

The LC-ESI-MS n method was developed for the analysis and characterization of alkaloids in the extract of E. rutaecarpa (called Wuzhuyu in Chinese). Thirty-six batches of Wuzhuyu from different locations of China were investigated and the common fingerprinting profile was established with a professional analytical software recommended by the State Food and Drug Administration. Fifteen chemical components of the common peaks were identified by multi-stage MS. The effects on the chromatographic profile resulting from different collecting locations, harvesting times or storage times were studied. Hierarchical clustering analysis and principal components analysis were also performed to classify and differentiate the 36 batches of the samples. As a result, those which had same chemical properties were sorted into one cluster, which was very useful in evaluating and controlling the quality of Wuzhuyu. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Ma F.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | Ma F.,Guizhou Normal University | Gong X.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | Gong X.,Guizhou Normal University | And 5 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Polygonum capitatum Buch.-Ham. ex D. Don has been traditionally used by Hmong for the treatments of urinary tract infections and pyelonephritis. Gallic acid (GA) and protocatechuic acid (PCA) are regarded as two of the main bioactive compounds in the herb. Materials and methods A rapid, selective and sensitive UHPLC-ESI-MS/MS method was established and validated for the quantification of GA and PCA in rat plasma after oral administration of P. capitatum extract. Concentrations of GA and PCA were determined at different time points after dosing 20 mg/kg (equivalent to 4 mg/kg of GA and 0.3 mg/kg of PCA), 60 mg/kg and 120 mg/kg of P. capitatum extract. The main pharmacokinetic parameters of GA and PCA were obtained based on the analysis of the plasma sample by non-compartmental analysis. Results After oral administration of P. capit]atum extract, GA and PCA were quickly absorbed and showed a dose-dependent profile. Pharmacokinetic parameters for GA and PCA following oral administration of the extract were respectively: Cmax 246.24-806.27 and 15.73-30.72 ng/mL; Tmax 40-100 and 20-40 min. In the rats treated with P. capitatum t1/2 and Tmax of GA were prolonged by comparing with that of its pure form. Conclusion Other compounds in P. capitatum extract may be metabolized to GA, which affected the pharmacokinetic profiles of GA. This pharmacokinetic study seems to be useful for a further clinical study of P. capitatum extract. © 2015 Published by Elsevier Ireland Ltd.


Zhao Y.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | Zhao Y.,Guizhou Normal University | Gong X.-J.,Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection | Gong X.-J.,Guizhou Normal University | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2014

A rapid, sensitive and reliable UHPLC-ESI-MS/MS method was developed for simultaneous determination of gastrodin and parishin in rat plasma. The LLOQ of the two analytes were 1.00×10-1 and 8.30×10-5μg/mL, respectively. The intra-day and inter-day precision were all less than 10% of the relative standard deviation (RSD), whilst the accuracy were all within ±15% of the relative error (RE). The proposed method was successfully applied for pharmacokinetics study on the two analytes in rats after oral administration of Gastrodiae rhizoma (GR) extract and powder at low, medium and high dosages. Blood samples were collected from the suborbital vein at predetermined time points and were precipitated using methanol. Chromatographic separations were carried out on a Kinetex XB-C18 column (2.1mm×150mm, 1.7μm) with a gradient mobile phase of acetonitrile-water with 0.1% formic acid as a modifier. The pharmacokinetic parameters of the two analytes in rats were obtained and the relative bioavailability of gastrodin and parishin in two formulations were calculated. The results indicated that higher bioavailability was obtained when low dosage of GR powder was used, whereas, higher bioavailability values were obtained when medium and high dosages of GR extract were used. © 2014.

Loading Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection collaborators
Loading Guizhou Key Laboratory for Mountainous Environmental Information and Ecological Protection collaborators