Li B.,Guizhou University |
Wei C.,Guizhou University |
Wen T.,Guizhou University |
Shi J.,Tobacco Science Institute of Guizhou |
And 2 more authors.
Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae | Year: 2012
In the study, some microbial strains were isolated from soil and manure samples. 3 highly efficient straw-degrading strains, SA2, MA6 and HA4, were then obtained by the experiments of filter collapse and transparent circle method. MA6 and SA2 are Trichoderma viride, HA4 is PeniciUium decumbens in appraisal. Through the dual culture method, we found that SA2 and HA4 were in good compatibility. Experiments were also carried out for optimizing the conditions of enzyme production. The results showed that the optimal conditions of enzyme generation for SA2+HA4 strains are the pH value of 5, the culture temperature of 30°C, the inoculum concentration of 3% and culture time of 72 h. Cellulase, protease and amylase activity are 193.5, 25.3 and 55.7 U·mL-1, respectively.