Guizhou Institute of Tobacco Science

Guiyang, China

Guizhou Institute of Tobacco Science

Guiyang, China
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Zhang Y.,Chinese Academy of Agricultural Sciences | Liu Q.,Chinese Academy of Agricultural Sciences | Wang J.,CAS Zhengzhou Research Institute | Yin Q.,CAS Zhengzhou Research Institute | And 4 more authors.
Tobacco Science and Technology | Year: 2015

In order to reveal the relationship between flavor type of flue-cured tobacco and climate, a meteorological factor-based evaluation model for flue-cured tobacco flavor types was established with stepwise discriminant analysis method involving the meteorological data of 33 typical regions of different flue-cured tobacco flavor types. The model was verified with 480 flue-cured tobacco samples planted across China in 2012. The results showed that the discriminating accuracy of the model was 86.2%. Using the meteorological factors of major flue-cured tobacco growing areas in China and the flavor type of tobacco leaves from each area discriminated by the model, a flavor type map of flue-cured tobacco was obtained, it clarified the distribution areas of flue-cured tobacco of robust, medium and fresh flavor styles. © 2015, Editorial Office of Tobacco Science & Technology. All right reserved.


Guo Y.,Guizhou Institute of Tobacco Science | Liu H.,CAS Institute of Process Engineering | Yang Z.,Guizhou Institute of Tobacco Science | Chen J.,Guizhou Institute of Tobacco Science | And 2 more authors.
Gene | Year: 2012

Tobacco is one of the most important economic and agricultural crops worldwide. miRNAs have been increasingly acknowledged for their important roles in different biological processes of tobacco. However, few miRNAs have been identified so far in tobacco impeding the development of new tobacco strains with better properties. In this study, high-throughput sequencing technology was employed to identify novel tobacco miRNAs. A total of 84 potential miRNAs were obtained in tobacco, including 33 conserved and 51 novel miRNAs. Tissue-specific and topping-related miRNAs were identified. A tobacco miRNA microarray was also constructed to investigate miRNA expression patterns in different tissues, and their expression patterns were further validated by qRT-PCR and Northern Blot. Finally, the potential targets of these miRNAs were predicted based on a sequence homology search. Thus, in the current study, we have performed the comprehensive analysis of tobacco miRNAs, including their identification, expression pattern and target prediction. Our study opens a new avenue for further elucidation for their roles underlying the regulation of diversity of physiological processes. © 2012 Elsevier B.V..


Xiang Z.,Guizhou Institute of Tobacco Science | Cai K.,Guizhou Institute of Tobacco Science | Geng Z.,Guizhou Institute of Tobacco Science | Zhang J.,Guizhou Institute of Tobacco Science | Zhou S.,Guizhou Institute of Tobacco Science
Analytical Letters | Year: 2013

Simultaneous extraction and derivatization followed by head-space solid-phase microextraction (HS-SPME) coupled with gas chromatography/mass spectrometry (GC-MS) was employed to determine volatile organic acids (VOAs) in flue-cured tobacco samples. Several experimental parameters, such as the type of SPME fibers, sampling temperature, pre-extraction and derivatization equilibration time, and anhydrous sodium sulfate amount, were optimized to improve sampling efficiency. Validation was carried out in terms of limits of detection (LOD), precision, recovery, and linearity. The results indicated that the optimized technique was easy, highly efficient, and sensitive. Finally, the performance of the proposed method was tested for the extraction and determination of the VOAs in flue-cured tobacco and satisfactory results were achieved. © 2013, Taylor & Francis Group, LLC.


Ma Y.-N.,Zhejiang University | Wang R.-G.,Guizhou Institute of Tobacco Science | Wu C.,Guizhou Institute of Tobacco Science | Sh Y.-W.,Guizhou Institute of Tobacco Science | And 5 more authors.
International Journal of Agriculture and Biology | Year: 2012

Analysis of genetic main effects and genotype × environment interaction effects for total sugar and reducing sugar of flue-cured tobacco were conducted at two locations and diallel cross by using the additive, dominance and additive × additive model. Unconditional and conditional analyses were employed to investigate the developing behavior of quantitative traits and uncover the genetic mechanisms of flue-cured tobacco. Total and reducing sugars were greatly affected by genotype × environment interaction effect rather than genetic main effect, and gene intermittently expressed and exhibited different genetic mode in different growth stages. It was suggested that genetic improvement for total sugars of flue-cured tobacco could be achieved by selection of pure lines in the period of Gs1118 to Gs1121 in early generations and by selection of heterosis in the period before Gs1109 in advanced generations under multiple environments. As for RS, it would be better to select hybrid in the period of Gs1112 to Gs1121 in advanced generations under specific environments. © 2012 Friends Science Publishers.


Zhao J.-H.,Guizhou Institute of Tobacco Science | Zhang J.-S.,Guizhou Institute of Tobacco Science | Wang Y.,Guizhou Institute of Tobacco Science | Wang R.-G.,Guizhou Institute of Tobacco Science | And 3 more authors.
Journal of Zhejiang University: Science B | Year: 2011

DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic traits. © 2011 Zhejiang University and Springer-Verlag Berlin Heidelberg.


PubMed | Guizhou Institute of Tobacco Science
Type: Journal Article | Journal: Gene | Year: 2012

Tobacco is one of the most important economic and agricultural crops worldwide. miRNAs have been increasingly acknowledged for their important roles in different biological processes of tobacco. However, few miRNAs have been identified so far in tobacco impeding the development of new tobacco strains with better properties. In this study, high-throughput sequencing technology was employed to identify novel tobacco miRNAs. A total of 84 potential miRNAs were obtained in tobacco, including 33 conserved and 51 novel miRNAs. Tissue-specific and topping-related miRNAs were identified. A tobacco miRNA microarray was also constructed to investigate miRNA expression patterns in different tissues, and their expression patterns were further validated by qRT-PCR and Northern Blot. Finally, the potential targets of these miRNAs were predicted based on a sequence homology search. Thus, in the current study, we have performed the comprehensive analysis of tobacco miRNAs, including their identification, expression pattern and target prediction. Our study opens a new avenue for further elucidation for their roles underlying the regulation of diversity of physiological processes.


PubMed | Guizhou Institute of Tobacco Science
Type: Journal Article | Journal: Journal of Zhejiang University. Science. B | Year: 2011

DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic traits.

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