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Zeng Q.,Guizhou Institute of Prataculture | Huang H.,Chinese Academy of Sciences | Zhu J.,Chinese Academy of Sciences | Fang Z.,Chinese Academy of Sciences | And 2 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Strain HA10002 was isolated from mangrove sediment collected from Dongzhaigang Mangrove Reserve in Hainan, China. It was selected with potent nematicidal activity and was identified as Streptomyces albogriseolus. By bioassay-guided fractionation, a new active component A22-1(S1) against root-knot nematodes was separated from its fermentation broth. On the basis of spectroscopic analyses and comparison with the data from correlative literature, the structure of S1 was established to be 6′-methyl-fungichromin, named as fungichromin B in this paper. The LD50 values of fungichromin B to the 2-stage juveniles of Meloidogyne incognita and Meloidogyne javanica were 7.64 and 7.83 μg/ml, respectively. Further examination demonstrated fungichromin B still showed a wide antifungal spectrum, as with fungichromin. © 2013 The Author(s).

Zhang Y.,Sichuan Agricultural University | Yan H.,Sichuan Agricultural University | Jiang X.,Sichuan Agricultural University | Wang X.,Guizhou Institute of Prataculture | And 4 more authors.
Hereditas | Year: 2016

Background: To evaluate genetic variation, population structure, and the extent of linkage disequilibrium (LD), 134 switchgrass (Panicum virgatum L.) samples were analyzed with 51 markers, including 16 ISSRs, 20 SCoTs, and 15 EST-SSRs. Results: In this study, a high level of genetic variation was observed in the switchgrass samples and they had an average Nei's gene diversity index (H) of 0.311. A total of 793 bands were obtained, of which 708 (89.28 %) were polymorphic. Using a parameter marker index (MI), the efficiency of the three types of markers (ISSR, SCoT, and EST-SSR) in the study were compared and we found that SCoT had a higher marker efficiency than the other two markers. The 134 switchgrass samples could be divided into two sub-populations based on STRUCTURE, UPGMA clustering, and principal coordinate analyses (PCA), and upland and lowland ecotypes could be separated by UPGMA clustering and PCA analyses. Linkage disequilibrium analysis revealed an average r2 of 0.035 across all 51 markers, indicating a trend of higher LD in sub-population 2 than that in sub-population 1 (P < 0.01). Conclusions: The population structure revealed in this study will guide the design of future association studies using these switchgrass samples. © 2016 Zhang et al.

Yun S.X.,Chongqing University of Science and Technology | Ning X.K.,Guizhou Normal University | Li W.X.,Guizhou Institute of Prataculture | Bi C.Y.,Guizhou Normal University | Meng Z.,Guizhou Normal University
Journal of Animal and Veterinary Advances | Year: 2014

The Oula sheep was affected by high level of sulfur in forage in the Huangheshouqu Pasture in Gansu Province in the South of the Qinghai-Tibetan Plateau of China. The related blood indices were measured. The mineral composition of forages, blood and liver in Oula sheep from ranches of high sulfur were compared with those of samples from normal areas. The concentration of copper in soil and forage was 16.8±7.1 and 6.39±1.26 μg g-1 (dry matter), respectively compared with 16.7±5.6 and 7.12±2.86 μg g-1. The mean concentration of Cu in blood and liver from the affected Oula sheep was 0.28±0.05 and 13.9±3.7 μg g-1, respectively compared with 0.86±0.16 and 107.6±11.2 μ g g-1 for non-affected Oula sheep. The concentration of sulfur in soil and forage was 1.97±0.26 and 1.36±0.17%, respectively compared with 10.8±0.31 and 0.57±0.16% (dry matter) for normal animals. The content of S in blood and liver was 6.37±1.7 and 1,58±3.6%, respectively compared with 4.12±0.86 and 1.32±0.35% (in fresh and soft tissues), respectively. The 12 Cu-deficient sheep were obtained from the same location allocated to one of two treatments, consisting of supplement providing 42 mg dayg-1 of Cu sulfate or tribasic copper chloride. Treatment were delivered for 90 days. Liver Cu increased over time in all sheep regardless of treatment, however, sheep supplemented with tribasic copper chloride tended (p<0.01) to have higher mean liver Cu concentrations than those receiving Cu sulfate. © Medwell Journals, 2014.

Wang P.,Guizhou Institute of Prataculture | Mo B.,Guizhou Institute of Prataculture | Long Z.,Guizhou Institute of Prataculture | Fan S.,Chinese Academy of Forestry | And 2 more authors.
Industrial Crops and Products | Year: 2016

Sophora davidii is a popular ecological and feeding plant, but it also is a potential medicinal plant. Its root has high levels of lupin alkaloids, which make a kind of valued matrine-type alkaloids as one of the best alternatives to pentazocine. Very limited agronomic information exists regarding the cultivation of S. davidii as a medicinal and industrial crop. The objective of this study was to investigate seed germination and emergence of S. davidii in response to temperature, osmotic potential, salinity and depth of seed burial. The effect of temperature, osmotic potential and salinity on seed germination were examined at different temperatures (5-40 °C), while using different polyethylene glycol (PEG)-6000 (0--1.0 MPa) and different CaCl2 (0-200 mM) solutions. Seedling emergence was also examined for seeds sown at different depths (0-9 cm). Temperature affected germination rates of S. davidii; maximum germination (30.67%) was reached at 25 days a constant temperature of 20 °C. Maximum germination rates were obtained under low osmotic potential (0--0.4 MPa) and low salinity concentrations (20-50 mM CaCl2); increasing osmotic potential or salinity progressively inhibited seed germination. Deep burial significantly decreased seedling emergence rates, seedlings emerged well at depths of 0-3 cm, but no seedlings emerged from seeds buried at depths of ≥9 cm. As a general recommendation to growers S. davidii should be seeded no deeper than 3-cm depth, and the suitable environmental conditions (temperatures of 20-30 °C, water stress of 0--0.4 MPa and salt stress of 20-50 mM CaCl2) may be encountered. © 2016 Elsevier B.V.

Wang X.-L.,Sichuan Agricultural University | Wang X.-L.,Guizhou Institute of Prataculture | Liu X.-X.,Guizhou Institute of Prataculture | Chen W.,Guizhou Institute of Prataculture | And 5 more authors.
Plant Biosystems | Year: 2012

Delay of floral transition is a valuable trait in commercially important turfgrass species. It has been reported that short vegetative phase (SVP)-like proteins, potential repressors of the floral inductive pathway, control the transition from vegetative to reproductive phase. A homolog of SVP-like genes was isolated from tall fescue (Festuca arundinacea) by 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE and designated as F. arundinacea VEGETATIVE TO REPRODUCTIVE TRANSITION gene-2 (FaVRT-2). It encodes a putative protein of 226 amino acids with a conserved MADS (M) domain, a Keratin-like (K) domain, and a C-terminal (C) domain. Phylogenetic analysis showed that FaVRT-2 is most closely related to VRT-2 proteins from ryegrass, barley, and wheat and belongs to the StMADS11 subfamily of MADS-box transcription factors. Subcellular localization showed that the FaVRT-2 protein accumulates in the nucleus suggesting a role as trans acting factor. FaVRT-2 transcripts accumulate abundantly in roots and stems and is moderately present in leaves under normal conditions (without non-vernalization treatment). Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed similar transcriptional patterns of FaVRT-2 under both vernalization and non-vernalization conditions. However, the peak of FaVRT-2 transcripts under vernalization condition occurred 14 days earlier than that found under non-vernalization condition. These results support the hypothesis that FaVRT-2, a putative repressor of floral transition, is regulated by vernalization in tall fescue. © 2012 Copyright Taylor and Francis Group, LLC.

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