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PubMed | Guizhou Institute of Prataculture, Sichuan University, Nanjing Agricultural University and Sichuan Agricultural University
Type: | Journal: Hereditas | Year: 2017

To evaluate genetic variation, population structure, and the extent of linkage disequilibrium (LD), 134 switchgrass (In this study, a high level of genetic variation was observed in the switchgrass samples and they had an average Neis gene diversity index (H) of 0.311. A total of 793 bands were obtained, of which 708 (89.28%) were polymorphic. Using a parameter marker index (MI), the efficiency of the three types of markers (ISSR, SCoT, and EST-SSR) in the study were compared and we found that SCoT had a higher marker efficiency than the other two markers. The 134 switchgrass samples could be divided into two sub-populations based on STRUCTURE, UPGMA clustering, and principal coordinate analyses (PCA), and upland and lowland ecotypes could be separated by UPGMA clustering and PCA analyses. Linkage disequilibrium analysis revealed an average rThe population structure revealed in this study will guide the design of future association studies using these switchgrass samples.


PubMed | Animal Husbandry and Veterinary Research Institute of Guizhou Province, Guizhou Institute of Prataculture, Southwest University and Sichuan Agricultural University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L


Yun S.X.,Chongqing University of Science and Technology | Ning X.K.,Guizhou Normal University | Li W.X.,Guizhou Institute of Prataculture | Bi C.Y.,Guizhou Normal University | Meng Z.,Guizhou Normal University
Journal of Animal and Veterinary Advances | Year: 2014

The Oula sheep was affected by high level of sulfur in forage in the Huangheshouqu Pasture in Gansu Province in the South of the Qinghai-Tibetan Plateau of China. The related blood indices were measured. The mineral composition of forages, blood and liver in Oula sheep from ranches of high sulfur were compared with those of samples from normal areas. The concentration of copper in soil and forage was 16.8±7.1 and 6.39±1.26 μg g-1 (dry matter), respectively compared with 16.7±5.6 and 7.12±2.86 μg g-1. The mean concentration of Cu in blood and liver from the affected Oula sheep was 0.28±0.05 and 13.9±3.7 μg g-1, respectively compared with 0.86±0.16 and 107.6±11.2 μ g g-1 for non-affected Oula sheep. The concentration of sulfur in soil and forage was 1.97±0.26 and 1.36±0.17%, respectively compared with 10.8±0.31 and 0.57±0.16% (dry matter) for normal animals. The content of S in blood and liver was 6.37±1.7 and 1,58±3.6%, respectively compared with 4.12±0.86 and 1.32±0.35% (in fresh and soft tissues), respectively. The 12 Cu-deficient sheep were obtained from the same location allocated to one of two treatments, consisting of supplement providing 42 mg dayg-1 of Cu sulfate or tribasic copper chloride. Treatment were delivered for 90 days. Liver Cu increased over time in all sheep regardless of treatment, however, sheep supplemented with tribasic copper chloride tended (p<0.01) to have higher mean liver Cu concentrations than those receiving Cu sulfate. © Medwell Journals, 2014.


Zhao L.-L.,Guizhou University | Mo B.-T.,Guizhou Institute of Prataculture | Wang P.-C.,Guizhou Institute of Prataculture | Zhang Y.,Guizhou Institute of Prataculture | Long Z.-F.,Guizhou Institute of Prataculture
South African Journal of Botany | Year: 2015

Sophora davidii is an important leguminous shrub that is widely used for revegetation in areas experiencing karst rocky desertification in southwest China. Seed size is a prominent life history trait affecting seed fate. Laboratory experiments were conducted to evaluate the effect of osmotic potential and seed size on germination, dormancy, and mortality. The osmotic potentials required for 50% inhibition of maximum germination of large, medium, and small size seeds were - 0.836, - 0.786, and - 0.812. MPa, respectively. The highest seed dormancy (70%) was observed in small seeds at - 1.0. MPa and the lowest seed mortality (6%) in large seeds at 0. MPa. Increasing osmotic potential caused a decrease in the germination of large and small seeds, but increased large seeds mortality. Under mild water stress, medium seed germination, large seed dormancy, and small seed mortality were higher, whereas medium and small seed dormancy was lower. Moreover, osmotic potential and seed size significantly affected seed germination, dormancy, and mortality, as well as the interactions between them. Our findings suggest that seed size plays an ecologically important role in the fate of S. davidii seeds by improving medium or small seed germination and reducing medium or small seed mortality in severe habitats of the karst rocky desertification region. © 2015 South African Association of Botanists.


Wang P.C.,Guizhou Institute of Prataculture | Zhao L.L.,Guizhou University | Mo B.T.,Guizhou Institute of Prataculture | Zhang Y.,Guizhou Institute of Prataculture | And 2 more authors.
Genetics and Molecular Research | Year: 2015

In order to investigate genetic diversity and population structure of Ceratoides arborescens, six populations were selected from different steppe types in Inner Mongolia grasslands of China. Intersimple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among natural populations of C. arborescens. Thirteen ISSR primers generated 154 discernible DNA bands, of which 151 (98.05%) were polymorphic. High genetic diversity was detected at the species level [percentage of polymorphic loci (PPB) = 98.05%; H = 0.2984; I = 0.4557], whereas, relatively low genetic diversity existed within populations (PPB = 80.62%; H = 0.2675; I = 0.4031). Analysis of molecular variance showed that variation existed mainly within populations (73.25%) rather than among populations (26.75%), which was in line with the high level of gene flow (Nm = 4.3332). The Mantel test found no significant correlation between genetic distance and geographic distance (r = 0.7522, P < 0.05). Six populations were clustered into two main groups, a desert steppe group and a typical steppe group. ©FUNPEC-RP.


Zeng Q.,Guizhou Institute of Prataculture | Huang H.,Chinese Academy of Sciences | Zhu J.,Chinese Academy of Sciences | Fang Z.,Chinese Academy of Sciences | And 2 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Strain HA10002 was isolated from mangrove sediment collected from Dongzhaigang Mangrove Reserve in Hainan, China. It was selected with potent nematicidal activity and was identified as Streptomyces albogriseolus. By bioassay-guided fractionation, a new active component A22-1(S1) against root-knot nematodes was separated from its fermentation broth. On the basis of spectroscopic analyses and comparison with the data from correlative literature, the structure of S1 was established to be 6′-methyl-fungichromin, named as fungichromin B in this paper. The LD50 values of fungichromin B to the 2-stage juveniles of Meloidogyne incognita and Meloidogyne javanica were 7.64 and 7.83 μg/ml, respectively. Further examination demonstrated fungichromin B still showed a wide antifungal spectrum, as with fungichromin. © 2013 The Author(s).


Wang P.C.,Guizhou Institute of Prataculture | Mo B.T.,Guizhou Institute of Prataculture | Chen Y.,Guizhou Institute of Prataculture | Zeng Q.F.,Guizhou Institute of Prataculture | Wang L.B.,Chinese Academy of Forestry
Genetics and Molecular Research | Year: 2016

Karst mountainous ecosystems are associated with karst rocky desertification (KRD), which can greatly impact soil structure and function. Despite the importance of soil microbes as a major factor maintaining ecosystem stability, we know little about the effect on soil fungal communities of KRD in karst regions. We investigated this relationship across a gradient of KRD soils from Guizhou, China by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE). Fungal diversity indices (Shannon-Wiener, richness, and evenness) significantly differed (P < 0.05) based on KRD severity, being lowest in moderately affected areas. Cluster analysis showed that the five sites examined clustered into two main groups according to KRD grade (high and low). Moreover, a homology search using sequences recovered from PCR-DGGE bands showed that the dominant fungi in each community varied remarkably, and included Aspergillus, Aphanoascus, Blastomyces, Fusarium, Glomus, Geomyces, Gibberella, Mortiere lla, Tetracladium, and Tumularia species, and an unclassified group. In conclusion, these findings demonstrate that KRD has a significant impact on soil fungal communities. © 2016 The Authors.


Wang P.,Guizhou Institute of Prataculture | Mo B.,Guizhou Institute of Prataculture | Long Z.,Guizhou Institute of Prataculture | Fan S.,Chinese Academy of Forestry | And 2 more authors.
Industrial Crops and Products | Year: 2016

Sophora davidii is a popular ecological and feeding plant, but it also is a potential medicinal plant. Its root has high levels of lupin alkaloids, which make a kind of valued matrine-type alkaloids as one of the best alternatives to pentazocine. Very limited agronomic information exists regarding the cultivation of S. davidii as a medicinal and industrial crop. The objective of this study was to investigate seed germination and emergence of S. davidii in response to temperature, osmotic potential, salinity and depth of seed burial. The effect of temperature, osmotic potential and salinity on seed germination were examined at different temperatures (5-40 °C), while using different polyethylene glycol (PEG)-6000 (0--1.0 MPa) and different CaCl2 (0-200 mM) solutions. Seedling emergence was also examined for seeds sown at different depths (0-9 cm). Temperature affected germination rates of S. davidii; maximum germination (30.67%) was reached at 25 days a constant temperature of 20 °C. Maximum germination rates were obtained under low osmotic potential (0--0.4 MPa) and low salinity concentrations (20-50 mM CaCl2); increasing osmotic potential or salinity progressively inhibited seed germination. Deep burial significantly decreased seedling emergence rates, seedlings emerged well at depths of 0-3 cm, but no seedlings emerged from seeds buried at depths of ≥9 cm. As a general recommendation to growers S. davidii should be seeded no deeper than 3-cm depth, and the suitable environmental conditions (temperatures of 20-30 °C, water stress of 0--0.4 MPa and salt stress of 20-50 mM CaCl2) may be encountered. © 2016 Elsevier B.V.


PubMed | Guizhou Institute of Prataculture
Type: Journal Article | Journal: Antonie van Leeuwenhoek | Year: 2013

Strain HA10002 was isolated from mangrove sediment collected from Dongzhaigang Mangrove Reserve in Hainan, China. It was selected with potent nematicidal activity and was identified as Streptomyces albogriseolus. By bioassay-guided fractionation, a new active component A22-1(S1) against root-knot nematodes was separated from its fermentation broth. On the basis of spectroscopic analyses and comparison with the data from correlative literature, the structure of S1 was established to be 6-methyl-fungichromin, named as fungichromin B in this paper. The LD50 values of fungichromin B to the 2-stage juveniles of Meloidogyne incognita and Meloidogyne javanica were 7.64 and 7.83 g/ml, respectively. Further examination demonstrated fungichromin B still showed a wide antifungal spectrum, as with fungichromin.


PubMed | Chinese Academy of Forestry and Guizhou Institute of Prataculture
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Karst mountainous ecosystems are associated with karst rocky desertification (KRD), which can greatly impact soil structure and function. Despite the importance of soil microbes as a major factor maintaining ecosystem stability, we know little about the effect on soil fungal communities of KRD in karst regions. We investigated this relationship across a gradient of KRD soils from Guizhou, China by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE). Fungal diversity indices (Shannon-Wiener, richness, and evenness) significantly differed (P < 0.05) based on KRD severity, being lowest in moderately affected areas. Cluster analysis showed that the five sites examined clustered into two main groups according to KRD grade (high and low). Moreover, a homology search using sequences recovered from PCR-DGGE bands showed that the dominant fungi in each community varied remarkably, and included Aspergillus, Aphanoascus, Blastomyces, Fusarium, Glomus, Geomyces, Gibberella, Mortierella, Tetracladium, and Tumularia species, and an unclassified group. In conclusion, these findings demonstrate that KRD has a significant impact on soil fungal communities.

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