Guizhou Engineering Research Center for Fruit Processing

Guiyang, China

Guizhou Engineering Research Center for Fruit Processing

Guiyang, China
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Luo D.-L.,South China Agricultural University | Luo D.-L.,Guizhou Engineering Research Center for Fruit Processing | Ba L.-J.,South China Agricultural University | Ba L.-J.,Guizhou Engineering Research Center for Fruit Processing | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2017

Phytohormone abscisic acid (ABA) and plant-specific WRKY transcription factors (TFs) have been implicated to play important roles in various stress responses. The involvement of WRKY TFs in ABA-mediated cold tolerance of economical fruits, such as banana fruit, however remains largely unknown. Here, we reported that ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents, and thereby enhance cold tolerance in banana fruit. Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized. All four of these MaWRKYs were nuclear-localized and displayed transactivation activities. Their expressions were induced by ABA treatment during cold storage. More importantly, the gel mobility shift assay and transient expression analysis revealed that MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71 directly bound to the W-box elements in MaNCED1 and MaNCED2 promoters and activated their expressions. Taken together, our findings demonstrate that banana fruit WRKY TFs are involved in ABA-induced cold tolerance by, at least in part, increasing ABA levels via directly activating NECD expressions. © 2017 American Chemical Society.


Wang R.,Guizhou Engineering Research Center for Fruit Processing | Chen S.-Y.,Guizhou Engineering Research Center for Fruit Processing | Xie G.-F.,Guizhou Engineering Research Center for Fruit Processing | Ma L.-Z.,Guizhou Engineering Research Center for Fruit Processing | And 4 more authors.
Modern Food Science and Technology | Year: 2014

In the present study, the respiration rate, ethylene production rate, rot rate, weight loss rate, color difference, soluble solid, total acid, total alcohol, total phenols and anthocyanin content of five different late-maturing blueberry varieties (Gardenblue, Baldwin, Powderblue, Tifblue and Brightwell) from Majiang (Guizhou) were determined, which were stored under 1±0.3°C for 75 days. And then the changes of storage quality were compared and analyzed. The result showed that the soluble solids, total phenols and anthocyanin content of five kinds of blueberry declined in the earlier days then rose with prolonging storage time. No obvious fluctuation was observed in total acid. The rot rate, weight loss rate and colour difference showed a rising trend. Among them, Tifblue showed the highest rot rate because of high respiration rate, water content, and soluble solid decline tendency; anthocyanin content of Gardenblue kept the highest level; the rot rate of Powderblue was the lowest. The sequence of storability was as follows: Powderblue>Brightwell>Baldwin>Gardenblue>Tifblue.


Wang R.,Guizhou Engineering Research Center for Fruit Processing | Hu X.-L.,Guizhou Engineering Research Center for Fruit Processing | Xie G.-F.,Guizhou Engineering Research Center for Fruit Processing | Ji N.,Guizhou Engineering Research Center for Fruit Processing | And 4 more authors.
Modern Food Science and Technology | Year: 2015

Organic calcium spray was applied to blueberry fruits of two cultivars, 'Gardenblue' and 'Powderblue', during the growth period to improve the preservation effect. The results showed that the calcium content of blueberry fruits was effectively increased with the organic calcium spray. The increases through the growth period in respiratory rate, ethylene production rate, malondialdehyde content, and soluble pectin content in the blueberry fruits of two cultivars were inhibited, while the activities of polyphenoloxidase (PPO), peroxidase (POD), and polygalacturonase (PG) were decreased. As a result, fruit senescence was delayed, and the rates of rotting and soft fruit were decreased. Compared with the controls, the rates of rotting and soft fruit of 'Powderblue' after treatment with the organic calcium spray were decreased by 7.41% and 5.62%, respectively, and those of 'Gardenblue' after treatment with organic calcium spray were decreased by 11.62% and 9.57%, respectively. With regard to fruit quality, the changes in the soluble solid content, total acids, soluble pectin content, total phenols, anthocyanin content, and weight loss rate of the treated fruits were inhibited to varying degrees. Apart from this, the peel color and flavor index of the fruits treated with organic calcium spray were also effectively maintained. In summary, application of the organic calcium spray during the growth period can improve the preservation of blueberry fruits of the two cultivars 'Gardenblue' and 'Powderblue'. ©, 2015, South China University of Technology. All right reserved.

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