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Wen Y.,Guizhou Normal University | Wen Y.,Guizhou Engineering Laboratory for Quality Control and Evaluation Technology of Medicine | Chen H.,Guizhou Normal University | Chen H.,Guizhou Engineering Laboratory for Quality Control and Evaluation Technology of Medicine | And 10 more authors.
RSC Advances | Year: 2015

Blackberry contains high amounts of anthocyanins, whose extraction method is closely related with anthocyanin content and antioxidant activity. The extraction yield and antioxidant capacity acted as the comprehensive evaluation indexes, and a Box-Behnken design (BBD) of response surface methodology (RSM) was employed to further optimize the microwave-assisted extraction (MAE) conditions for blackberry anthocyanins (BBAC). A significant correlation was found between the double indexes extraction yield and the antioxidant capacity (P < 0.01). The results showed that the optimized extraction conditions included a microwave power of 469 W, a solvent concentration of 52%, a liquid-solid ratio of 25 g mL-1, and a microwave time of 4 min. Under these conditions, the mean experimental value of the extraction yield (2.18 ± 0.06 mg g-1), ABTS assay (32.18 ± 1.54 μM TEAC per g) and DPPH assay (27.18 ± 1.33 μM TEAC per g) corresponded well with the predicted values. Moreover, these mean experimental values were 120% higher than those obtained during ethanol leaching extraction. This journal is © The Royal Society of Chemistry. Source


Chen H.,Guizhou Engineering Laboratory for Quality Control and Evaluation Technology of Medicine | Chen H.,Guizhou Normal University | Zhou X.,Guizhou Engineering Laboratory for Quality Control and Evaluation Technology of Medicine | Zhou X.,Guizhou Normal University | And 14 more authors.
PLoS ONE | Year: 2015

Objective: To more scientifically and reasonably control the quality of Huangqi Granules, preliminary studies on the pharmacodynamics and serum pharmacochemistry of this medicine were performed. DPPH and MTT experiments showed that water extracts of Huangqi Granules had good antioxidant activity and increased immunity. Timed blood samples collected 5 min, 15 min, and 30 min after oral administration of a set amount of Huangqi Granules were collected and tested using UPLC-ESI-MS/MS. As a result, calycosin-7-O-β-D-glucoside, ononin, calycosin, astragaloside IV, and formononetin were found to exist in rat blood after dosing, indicating that the five chemical compounds might have pharmacological activity, and based on this result, they were designated biomarkers for quality control of Huangqi Granules. Consequently, a simple, rapid and efficient method was developed in the present study for the simultaneous determination of the five characteristic compounds in Huangqi Granules using HPLC-DAD-ELSD. Materials and Methods: The separation was performed using an Agilent Hypersil ODS column (4.6 x 250 mm, 5 μm) at 30°C. The mobile phase was composed of water (solvent A) and acetonitrile (solvent B) with a flow rate of 1 mL/min. The drift tube temperature of the ELSD system was set to 85°C, and the nitrogen pressure was 3.5 bar. Results: All five characteristic compounds had good linear behavior with r2 values greater than 0.9972. The recoveries varied from 96.31% to 101.22%. Subsequently, the developed method was applied to evaluate the quality of Huangqi Granules from different batches, and hierarchical clustering analysis (HCA) was used to analyze the classification of the samples based on the values of the five compounds. Conclusion: The established HPLC method combined with HCA proved to be effective to evaluate the quality of Huangqi Granules. © 2015 Chen et al. Source

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