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Ruo-Yu L.,Guizhou University | Tian T.,Guizhou University | Shi-Hui L.,Guizhou University | Bo H.,Guizhou Animal Husbandry and Veterinary Research Institute | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

To study the bovine mammary gland bioreactor, the 5′ and 3′ flanking sequence of bovine p-casein (CSN2) gene were cloned and connected to the eukaryotic expression vector of pC DNA3.0 which had been modified. The human Lysozyme gene (hLYZ) was inserted into the site between 5′ and 3′ flanking sequence after the vector named pC was identified and formed the vector named as pC-hLYZ subsequently. To determine whether the constructed vector could drive the hLYZ expression, the pC-hLYZ and positive control hLYZ plasmid were wrapped by the PEI and injected into lactation rabbits through mammary gland centre duct. Milk was collected and the expression status was detected after 72 h The plate inhibition assay showed that pC-hL YZ eukaryotic expression vector could be a good driver for hLYZ gene expression in rabbit mammary gland. The results demonstrated that the bovine mammary gland specific expression vector of hLYZ had been constructed successfully. The establishment of the vector laid the foundation for further study of bovine mammary gland expression technology. © Medwell Journals, 2012. Source

Chen H.,Guizhou Animal Husbandry and Veterinary Research Institute | Zhang J.,Guizhou Animal Husbandry and Veterinary Research Institute | Yu B.,Guizhou Animal Husbandry and Veterinary Research Institute | Li L.,Guizhou Animal Husbandry and Veterinary Research Institute | Shang Y.,Guizhou Animal Husbandry and Veterinary Research Institute
Gene | Year: 2015

The vertebrate fast skeletal troponin T (TNNT3) protein is an important regulatory and structural component of thin filaments in skeletal muscle, which improves meat quality traits of livestock and poultry. In this study, the troponin T isoforms from adult goat (skeletal muscle mRNA) were identified. We isolated the full-length coding sequence of the goat TNNT3 gene (GenBank: KM042888), analyzed its structure, and investigated its expression in different tissues from different aged goats (10, 30, 90, 180, and 360 days old). Real-time quantitative reverse transcription-polymerase chain reaction analyses revealed that Guizhou black goat TNNT3 was highly expressed in the biceps femoris muscle, abdominal muscle, and longissimus dorsi muscle (P < 0.01), and lowly expressed in the cardiac muscle, masseter muscle, and rumen tissue (P > 0.05). Western blotting confirmed that the TNNT3 protein was expressed in the muscle tissues listed above, with the highest level found in the longissimus dorsi muscle, and the lowest level in the masseter muscle. In the 10 to 360 day study period the TNNT3 protein expression level was the highest when the goats were 30 days old. A peptide, ASPPPAEVPEVHEEVH that may contribute to improved goat meat tenderness was identified. This study provides an insight into the molecular structure of the vertebrate TNNT3 gene. © 2015 Elsevier B.V. Source

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