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Hong N.-N.,Guiyang Medical University | Tao L.,Guizhou Animal Disease Control Center | Deng-Xiang Z.,Guizhou Animal Disease Control Center | Long-Zhong R.,Guizhou Animal Disease Control Center | Xiu-Gang Y.,Guizhou Animal Disease Control Center
Journal of Animal and Veterinary Advances | Year: 2012

Preparing biological tissue samples is an extremely tedious and time-consuming laboratory task, albeit one critical for rapid diagnoses of infectious diseases. Here, researchers reported a novel, low-cost sampling technique for rapid and accurate removal of minute tissue portions from various animal organs. Each sampled tissue portion could be easily dispersed and homogenized in storage solution in 2.0 mL microcentrifuge tube and was sufficient for the molecular diagnosis of the presence or absence of infectious disease using the Polymerase Chain Reaction (PCR) technique. This new sampling technique is beneficial as it provides both uniformity for high-throughput sampling and minimization of cross-contamination. © Medwell Journals, 2012.


Luo X.-F.,Guizhou Animal Disease Control Center | Hong N.-N.,Guiyang Medical University
Journal of Animal and Veterinary Advances | Year: 2014

The 946 serum samples were tested for antibodies against three porcine infectious agents in this study. The results showed the presence of antibodies to Salmonella in 53.70% of samples (508/946) to Trichinella in 3.81% of samples (36/946) to Yersinia in 65.33% of samples (618/946) and relevant herd positive rates were 54.55% (30/55), 7.27% (4/55) and 67.27% (37/55) correspondingly. Scaled farms or total farms (scaled and individual) displayed lower seroprevalence for Trichinella of 1.88% (10/532) or 2.44% (16/656) when compared to 6.90% (20/290) for abattoirs (p≤0.01) and individual farms showed lower seroprevalence for Yersinia of 37.10% (46/124) when compared to 71.80% (382/532) for scaled farms and 65.52% (190/290) for abattoirs (p<0.01). These demonstrated specific circulation of porcine Salmonella, Trichinella and Yersinia among pigs in some regions of Guizhou Province. © Medwell Journals, 2014.


Hong N.,Guizhou Animal Disease Control Center | De-Xing Q.,Guizhou Animal Disease Control Center | Deng-Xiang Z.,Guizhou Animal Disease Control Center | Long-Zhong R.,Guizhou Animal Disease Control Center
Journal of Animal and Veterinary Advances | Year: 2012

Simple method for the detection of Mycoplasma bovis in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO 2 incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, M. bovis grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay. © 2012 Medwell Journals.

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