Guizhou Academy of Tobacco Science

Guiyang, China

Guizhou Academy of Tobacco Science

Guiyang, China
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Lin S.,Guizhou Academy of Tobacco Science | Yu J.,Guizhou Academy of Tobacco Science | Wang Z.,Guizhou Academy of Tobacco Science | Ren X.,Guizhou Academy of Tobacco Science | Wang R.,Guizhou Academy of Tobacco Science
Tobacco Science and Technology | Year: 2016

To identify the genes related to the regulation of early flowering at low temperature in tobacco, two full-length cDNAs encoding different E3 ubiquitin ligase genes (NtHOS1a and NtHOS1b) were cloned from Nicotiana tabacum by bioinformatics, RT-PCR and SMART RACE technologies. GenBank accession numbers of NtHOS1a and NtHOS1b were KU558689 and KU558690, respectively. The putative full-length cDNA sequences of NtHOS1a and NtHOS1b were 3 599 bp and 3 578 bp, and these two genes encoded the predicted polypeptide of 963 and 954 amino acids, respectively. Amino acids identity between NtHOS1a and NtHOS1b was 95%. Putative polypeptide in the two genes matched Arabidopsis thaliana HOS1(NP_181511)polypeptide sequence with the identity of 51% and 50%, respectively. The two proteins shared highly conserved N-terminal RING domains of E3 ubiquitin ligases. qRT-PCR showed that under normal growing condition, NtHOS1a and NtHOS1b transcripts accumulated to a high level in root, stem and leaves. After 10 days of 12℃ treatment, the expression levels of the two genes were decreased to different degrees, this suggested that NtHOS1a and NtHOS1b expressions in tobacco root, stem and leaves were negatively regulated by low temperature. © 2016, Editorial Office of Tobacco Science & Technology. All right reserved.

Li Z.,China Agricultural University | Li Z.,Guizhou Academy of Tobacco Science | Zhang J.,Guizhou Academy of Tobacco Science | Liu Y.,Guizhou University | And 5 more authors.
BMC plant biology | Year: 2016

BACKGROUND: Auxin was recognized as a secondary dormancy phytohormone, controlling seed dormancy and germination. However, the exogenous auxin-controlled seed dormancy and germination remain unclear in physiological process and gene network.RESULTS: Tobacco seeds soaked in 1000 mg/l auxin solution showed markedly decreased germination compared with that in low concentration of auxin solutions and ddH2O. Using an electron microscope, observations were made on the seeds which did not unfold properly in comparison to those submerged in ddH2O. The radicle traits measured by WinRHIZO, were found to be also weaker than the other treatment groups. Quantified by ELISA, there was no significant difference found in β-1,3glucanase activity and abscisic acid (ABA) content between the seeds imbibed in gradient concentration of auxin solution and those soaked in ddH2O. However, gibberellic acid (GA) and auxin contents were significantly higher at the time of exogenous auxin imbibition and were gradually reduced at germination. RNA sequencing (RNA-seq), revealed that the transcriptome of auxin-responsive dormancy seeds were more similar to that of the imbibed seeds when compared with primary dormancy seeds by principal component analysis. The results of gene differential expression analysis revealed that auxin-controlled seed secondary dormancy was associated with flavonol biosynthetic process, gibberellin metabolic process, adenylyl-sulfate reductase activity, thioredoxin activity, glutamate synthase (NADH) activity and chromatin regulation. In addition, auxin-responsive germination responded to ABA, auxin, jasmonic acid (JA) and salicylic acid (SA) mediated signaling pathway (red, far red and blue light), glutathione and methionine (Met) metabolism.CONCLUSIONS: In this study, exogenous auxin-mediated seed secondary dormancy is an environmental model that prevents seed germination in an unfavorable condition. Seeds of which could not imbibe normally, and radicles of which also could not develop normally and emerge. To complete the germination, seeds of which would stimulate more GA synthesis to antagonize the stimulation of exogenous auxin. Exogenous auxin regulates multi-metabolic networks controlling seed secondary dormancy and germination, of which the most important thing was that we found the auxin-responsive seed secondary dormancy refers to epigenetic regulation and germination to enhance Met pathway. Therefore, this study uncovers a previously unrecognized transcriptional regulatory networks and physiological development process of seed dormancy and germination with superfluous auxin signal activate.

PubMed | Guizhou Academy of Tobacco Science, China Institute of Technology and Fujian Normal University
Type: | Journal: Antonie van Leeuwenhoek | Year: 2016

Pseudomonas sp., which occupy a variety of ecological niches, have been widely studied for their versatile metabolic capacity to promote plant growth, suppress microbial pathogens, and induce systemic resistance in plants. In this study, a Pseudomonas sp. strain p21, which was isolated from tomato root endophytes, was identified as having antagonism against Aspergillus niger. Further analysis showed that this strain had the ability to biosynthesise siderophores and was less effective in inhibiting the growth of A. niger with the supplementation of Fe

Zhao P.,Wuhan University | Zhou X.-M.,Wuhan University | Zou J.,Guizhou Academy of Tobacco Science | Wang W.,Wuhan University | And 3 more authors.
Journal of Experimental Botany | Year: 2014

Cystatins are tightly bound and reversible inhibitors of cysteine proteases in C1A and C13 peptidase families, which have been identified in several species and shown to function in vegetative development and response to biotic/abiotic stresses in plants. Recent work revealed their critical role in regulating programmed cell death during embryogenesis in tobacco and suggested their more comprehensive roles in the process of sexual plant reproduction, although little is known about cystatin family genes in the processes. Here, 10 cystatin family genes in Nicotiana tabacum were identified using an expressed sequence tag (EST)-based gene clone strategy. Analysis of their biochemical properties showed that nine of them have the potency to inhibit the activities of both commercial cathepsin L-like proteases and extracted cysteine proteases from seeds, but with different Ki values depending on the types of proteases and the developmental stages of the seed tested. This suggests that cystatin-dependent cathepsin L-like proteolytic pathways are probably important for early seed development. Comprehensive expression profile analysis revealed that cystatin family genes showed manifold variations in their transcription levels in different plant cell types, including the sperm, egg, and zygote, especially in the embryo and seed at different developmental stages. More interestingly, intracellular localization analysis of each cystatin revealed that most members of cystatin families are recognized as secretory proteins with signal peptides that direct them to the endoplasmic reticulum. These results suggest their widespread roles in cell fate determination and cell-cell communication in the process of sexual reproduction, especially in gamete and embryo development, as well as in seed formation. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Zhou X.-M.,Wuhan University | Zhao P.,Wuhan University | Wang W.,Wuhan University | Zou J.,Guizhou Academy of Tobacco Science | And 3 more authors.
DNA Research | Year: 2015

Autophagy is an evolutionarily conserved mechanism in both animals and plants, which has been shown to be involved in various essential developmental processes in plants. Nicotiana tabacum is considered to be an ideal model plant and has been widely used for the study of the roles of autophagy in the processes of plant development and in the response to various stresses. However, only a few autophagy-related genes (ATGs) have been identified in tobacco up to now. Here, we identified 30 ATGs belonging to 16 different groups in tobacco through a genome-wide survey. Comprehensive expression profile analysis reveals an abroad expression pattern of these ATGs, which could be detected in all tissues tested under normal growth conditions. Our series tests further reveal that majority of ATGs are sensitive and responsive to different stresses including nutrient starvation, plant hormones, heavy metal and other abiotic stresses, suggesting a central role of autophagy, likely as an effector, in plant response to various environmental cues. This work offers a detailed survey of all ATGs in tobacco and also suggests manifold functions of autophagy in both normal plant growth and plant response to environmental stresses. © 2015 The Author 2015. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

Hai-Ming T.,Hunan Soil and Fertilizer Institute | Xiao-Ping X.,Hunan Soil and Fertilizer Institute | Wen-Guang T.,Hunan Soil and Fertilizer Institute | Ye-Chun L.,Guizhou Academy of Tobacco Science | And 2 more authors.
PloS one | Year: 2014

Residue management in cropping systems is useful to improve soil quality. However, the studies on the effects of residue management on the enzyme activities and microbial community of soils in South China are few. Therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (Oryza sativa L.) system on soil enzyme activities and microbial community in Southern China fields were studied. The experiment has conducted at the experimental station of the Institute of Soil and Fertilizer Research, Hunan Academy of Agricultural Science, China since winter 2004. Four winter cropping systems were used: rice-rice-ryegrass (Lolium multiflorum L.) (R-R-Ry), rice-rice-Chinese milk vetch (Astragalus sinicus L.) (R-R-Mv), rice-rice-rape (Brassica napus L.) (R-R-Ra) and rice-rice with winter fallow (R-R-Fa). The result indicated that the enzyme activities in the R-R-Ry, R-R-Mv and R-R-Ra systems were significantly higher (P<0.05) than in the R-R-Fa system during the early and late rice season. The β-glucosidase activities reached peak values at the tillering stage after residue application, and alkaline phosphatase activities reached peak values at the booting stage after residue application, respectively, the activities of β-glucosidase and alkaline phosphatase gradually decreased after this. Arylsulfatase activities reached peak values at the maturity stage. Arylamidase activities reached peak values at the maturity stage. The numbers of aerobic bacteria, actinomycete and fungus of residue treatments were significantly higher (P<0.05) than that the R-R-Ra system. However, the number of anaerobic bacteria under the R-R-Ry and R-R-Mv systems was significantly lower (P<0.05) than that under the R-R-Fa system during early rice and late rice growth stage. Thus, incorporation of winter cover crops into rotations may increase enzyme activities and microbial community in soil and therefore improve soil quality.

Cai L.,Guizhou Academy of Tobacco Science | Lu N.,Guizhou Academy of Tobacco Science | Jia M.,Guizhou Academy of Tobacco Science | Shi J.,Guizhou Academy of Tobacco Science
Tobacco Science and Technology | Year: 2015

Bacterial wilt caused by Ralstonia solanacearum is one of major diseases for flue-cured tobacco production in Southern China. Ralstonia solanacearum is a complex species that infects more than 200 plant species belonging to more than 50 botanical families, including important crops such as potato, tomato, pepper, tobacco and banana. It is difficult to prevent and control the bacterial wilt of crops because the pathogen Ralstonia solanacearum contains many virulence factors and pathogenesis pathways. The purpose of this study was to investigate the potential of using phages to infect Ralstonia solanacearum, and thus, control the pathogenesis of Ralstonia solancearum. The phage φPB2 was further isolated from the rhizosphere of flue-cured tobacco roots from the soils of tobacco-planting field located in Guizhou province, which exhibited a broad range of host for Ralstonia solanacearum strains isolated from flue-cured tobacco in different counties in Guizhou province. Phage-host interaction experiments showed that phage φPB2 was infectious and lytic against Ralstonia solanacearum strains Rs1.70 and Rs1.74, which were isolated from peanuts and potatoes respectively. Plaque assay indicated that the titer of phage ϕ PB2 reached 108 pfu (plaque-forming unit)/mL, and phage φPB2 was stable in a wide range of temperatures between 4℃ and 45℃, and could survive up to 50 days in water or tobacco plants after inoculation at environmental temperature. These results suggested that phage φPB2 might be used as a potential agent in controlling the bacterial wilt of flue-cured tobacco. © 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

PubMed | Guizhou Academy of Tobacco Science, University Of Maiduguri, Zhejiang Academy of Agricultural Sciences and Zhejiang University
Type: Journal Article | Journal: Plant biology (Stuttgart, Germany) | Year: 2016

In the present study, a consortium of two rhizobacteria Bacillus amyloliquefaciens Bk7 and Brevibacillus laterosporus B4, termed BB, biochemical elicitors salicylic acid and -aminobutyric acid (SB) and their mixture (BBSB) were investigated for cold and drought stress tolerance in rice plants. After withholding water for 16days, rice plants treated with BBSB showed 100% survival, improved seedling height (35.4cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting (4%), necrosis (6%) and rolling of leaves. Similarly, BB inoculation enhanced plant growth and reduced overall symptoms in rice seedlings subjected to 05C for 24h. Our results imply several mechanisms underlying BB- and BBSB-elicited stress tolerance. In contrast to the control, both treatments significantly decreased leaf monodehydroascorbate (MDA) content and electrolyte leakage, and increased leaf proline and cholorophyll content. Moreover, activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) increased 3.0- and 3.6-fold, respectively. Moreover, expression of OsMYB3R-2, OsDIL, OsDREB1A and OsCDPK13 genes was significantly up-regulated, suggesting that these genes play important roles in abiotic stress tolerance of rice. In addition, bacterial strains Bk7 and B4 were able to produce high amounts of IAA and siderophores, and colonise the plant roots, while only strain Bk7 exhibited the capability to form biofilms and solubilise inorganic phosphate. This study indicates that the BB and BBSB bio-formulations can be used to confer induced systematic tolerance and improve the health of rice plants subject to chilling and drought stress.

PubMed | Yangtze University, Guizhou Academy of Tobacco science and University of Guelph
Type: | Journal: Pesticide biochemistry and physiology | Year: 2016

Azoxystrobin and kresoxim-methyl are strobilurin fungicides, and are effective in controlling many plant diseases, including Fusarium wilt. The mode of action of this kind of chemical is inhibition of respiration. This research investigated the sensitivities of Fusarium kyushuense to azoxystrobin and kresoxim-methyl, and to the alternative oxidase inhibitor salicylhydroxamic acid (SHAM). The Biolog FF MicroPlate is designed to examine substrate utilization and metabolic profiling of micro-organisms, and was used here to study the activity of azoxystrobin, kresoxim-methyl and SHAM against F. kyushuense. Results presented that azoxystrobin and kresoxim-methyl strongly inhibited conidial germination and mycelial growth of F. kyushuense, with EC50 values of 1.60 and 1.79gml(-1), and 6.25 and 11.43gml(-1), respectively; while not for SHAM. In the absence of fungicide, F. kyushuense was able to metabolize 91.6% of the tested carbon substrates, including 69 effectively and 18 moderately. SHAM did not inhibit carbon substrate utilization. Under the selective pressure of azoxystrobin and kresoxim-methyl during mycelial growth (up to 100gml(-1)) and conidial germination (up to 10gml(-1)), F. kyushuense was unable to metabolize many substrates in the Biolog FF MicroPlate; while especially for carbon substrates in glycolysis and tricarboxylic acid cycle, with notable exceptions such as -hydroxybutyric acid, y-hydroxybutyric acid, -ketoglutaric acid, -d-glucose-1-phosphate, d-saccharic acid and succinic acid in the mycelial growth stage, and -hydroxybutyric acid, y-hydroxybutyric acid, -ketoglutaric acid, tween-80, arbutin, dextrin, glycerol and glycogen in the conidial germination stage. This is a new finding for some effect of azoxystrobin and kresoxim-methyl on carbon substrate utilization related to glycolysis and tricarboxylic acid cycle and other carbons, and may lead to future applications of Biolog FF MicroPlate for metabolic effects of other fungicides and other fungi, as well as providing a carbon metabolic fingerprint of F. kyushuense that could be useful for identification.

PubMed | Yangtze University, Guizhou Academy of Tobacco Science and University of Guelph
Type: | Journal: Scientific reports | Year: 2016

Tobacco grey mold caused by Botrytis cinerea is an important fungal disease worldwide. Boscalid, carbendazim, iprodione, pyrimethanil and propiconazole are representative botryticides for grey mold management. This research investigated the sensitivities of B. cinerea from tobacco to these chemicals using the Biolog FF Microplate. All five chemicals showed inhibitory activity, with average EC50 values of 0.94, 0.05, 0.50, 0.61 and 0.31g ml(-1), respectively. B. cinerea metabolized 96.8% of tested carbon sources, including 29 effectively and 33 moderately, but the metabolic fingerprints differed under pressures imposed by these botryticides. For boscalid, B. cinerea was unable to metabolize many substrates related to tricarboxylic acid cycle. For carbendazim, carbon sources related to glycolysis were not metabolized. For iprodione, use of most carbon substrates was weakly inhibited, and the metabolic profile was similar to that of the control. For propiconazole, no carbon substrates were metabolized and the physiological and biochemical functions of the pathogen were totally inhibited. These findings provide useful information on metabolic activities of these botryticides, and may lead to future applications of the Biolog FF Microplate for examining metabolic effects of other fungicides on other fungi, as well as providing a metabolic fingerprint of B. cinerea that could be useful for identification.

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